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Protein

DNA alpha-glucosyltransferase

Gene

agt

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of glucose from uridine diphosphoglucose to 5-hydroxymethyl cytosine of T4 DNA to yield glucosyl 5-hydroxymethyl cytosine (glc-HMC) (PubMed:15381072). This DNA process seems to occur immediately after DNA synthesis since the DNA alpha-glucosyltransferase interacts with the clamp protein gp45 (PubMed:15381072). The glc-HMC modification protects the phage genome against its own nucleases and the host restriction endonuclease system (PubMed:15381072). The glc-HMC modification also protects against the host CRISPR-Cas9 defense system (PubMed:26081634).2 Publications

Catalytic activityi

Transfers an alpha-D-glucosyl residue from UDP-glucose to a hydroxymethylcytosine residue in DNA.1 Publication

Pathwayi: DNA modification

This protein is involved in the pathway DNA modification, which is part of Genetic information processing.
View all proteins of this organism that are known to be involved in the pathway DNA modification and in Genetic information processing.

GO - Molecular functioni

  • DNA alpha-glucosyltransferase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

CRISPR-cas system evasion by virus, Host-virus interaction, Restriction-modification system evasion by virus

Enzyme and pathway databases

UniPathwayiUPA00198.

Protein family/group databases

CAZyiGT72. Glycosyltransferase Family 72.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA alpha-glucosyltransferase (EC:2.4.1.261 Publication)
Short name:
AGT
Short name:
Alpha-GT
Gene namesi
Name:agt
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000009087 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001649411 – 400DNA alpha-glucosyltransferaseAdd BLAST400

Proteomic databases

PRIDEiP04519.

Interactioni

Subunit structurei

Interacts with clamp protein gp45.2 Publications

Structurei

Secondary structure

1400
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 7Combined sources6
Helixi15 – 29Combined sources15
Beta strandi33 – 39Combined sources7
Turni45 – 48Combined sources4
Beta strandi49 – 51Combined sources3
Turni53 – 55Combined sources3
Beta strandi57 – 59Combined sources3
Turni61 – 63Combined sources3
Helixi65 – 72Combined sources8
Beta strandi76 – 82Combined sources7
Helixi90 – 102Combined sources13
Beta strandi107 – 113Combined sources7
Helixi118 – 121Combined sources4
Beta strandi124 – 126Combined sources3
Helixi127 – 133Combined sources7
Beta strandi135 – 140Combined sources6
Beta strandi142 – 144Combined sources3
Helixi145 – 148Combined sources4
Helixi150 – 154Combined sources5
Beta strandi161 – 163Combined sources3
Beta strandi171 – 173Combined sources3
Helixi180 – 187Combined sources8
Helixi191 – 193Combined sources3
Beta strandi195 – 202Combined sources8
Helixi207 – 209Combined sources3
Helixi211 – 220Combined sources10
Turni221 – 226Combined sources6
Beta strandi228 – 232Combined sources5
Helixi238 – 245Combined sources8
Beta strandi250 – 253Combined sources4
Helixi255 – 260Combined sources6
Beta strandi264 – 266Combined sources3
Beta strandi269 – 273Combined sources5
Helixi277 – 285Combined sources9
Beta strandi287 – 292Combined sources6
Helixi298 – 300Combined sources3
Helixi307 – 315Combined sources9
Beta strandi317 – 322Combined sources6
Helixi323 – 328Combined sources6
Turni332 – 334Combined sources3
Helixi338 – 340Combined sources3
Beta strandi346 – 348Combined sources3
Helixi350 – 352Combined sources3
Helixi353 – 364Combined sources12
Helixi367 – 385Combined sources19
Helixi387 – 398Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XV5X-ray1.73A1-400[»]
1Y6FX-ray2.40A/B1-400[»]
1Y6GX-ray2.80A/B1-400[»]
1Y8ZX-ray1.90A/B1-400[»]
1YA6X-ray2.40A/B1-400[»]
ProteinModelPortaliP04519.
SMRiP04519.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04519.

Family & Domainsi

Family and domain databases

InterProiIPR016223. DNA_alpha-glucosyltransferase.
[Graphical view]
PfamiPF11440. AGT. 1 hit.
[Graphical view]
PIRSFiPIRSF000471. DNA_alpha-glucosyltransferase. 1 hit.

Sequencei

Sequence statusi: Complete.

P04519-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRICIFMARG LEGCGVTKFS LEQRDWFIKN GHEVTLVYAK DKSFTRTSSH
60 70 80 90 100
DHKSFSIPVI LAKEYDKALK LVNDCDILII NSVPATSVQE ATINNYKKLL
110 120 130 140 150
DNIKPSIRVV VYQHDHSVLS LRRNLGLEET VRRADVIFSH SDNGDFNKVL
160 170 180 190 200
MKEWYPETVS LFDDIEEAPT VYNFQPPMDI VKVRSTYWKD VSEINMNINR
210 220 230 240 250
WIGRTTTWKG FYQMFDFHEK FLKPAGKSTV MEGLERSPAF IAIKEKGIPY
260 270 280 290 300
EYYGNREIDK MNLAPNQPAQ ILDCYINSEM LERMSKSGFG YQLSKLNQKY
310 320 330 340 350
LQRSLEYTHL ELGACGTIPV FWKSTGENLK FRVDNTPLTS HDSGIIWFDE
360 370 380 390 400
NDMESTFERI KELSSDRALY DREREKAYEF LYQHQDSSFC FKEQFDIITK
Length:400
Mass (Da):46,704
Last modified:August 13, 1987 - v1
Checksum:i09CC1D4800F5AD2E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01804 Genomic DNA. Translation: CAA25940.1.
M10160 Genomic DNA. Translation: AAC05390.1.
AF158101 Genomic DNA. Translation: AAD42527.1.
PIRiA00577. XUBPA4.
RefSeqiNP_049673.1. NC_000866.4.

Genome annotation databases

GeneIDi1258823.
KEGGivg:1258823.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01804 Genomic DNA. Translation: CAA25940.1.
M10160 Genomic DNA. Translation: AAC05390.1.
AF158101 Genomic DNA. Translation: AAD42527.1.
PIRiA00577. XUBPA4.
RefSeqiNP_049673.1. NC_000866.4.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XV5X-ray1.73A1-400[»]
1Y6FX-ray2.40A/B1-400[»]
1Y6GX-ray2.80A/B1-400[»]
1Y8ZX-ray1.90A/B1-400[»]
1YA6X-ray2.40A/B1-400[»]
ProteinModelPortaliP04519.
SMRiP04519.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT72. Glycosyltransferase Family 72.

Proteomic databases

PRIDEiP04519.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1258823.
KEGGivg:1258823.

Enzyme and pathway databases

UniPathwayiUPA00198.

Miscellaneous databases

EvolutionaryTraceiP04519.

Family and domain databases

InterProiIPR016223. DNA_alpha-glucosyltransferase.
[Graphical view]
PfamiPF11440. AGT. 1 hit.
[Graphical view]
PIRSFiPIRSF000471. DNA_alpha-glucosyltransferase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGSTA_BPT4
AccessioniPrimary (citable) accession number: P04519
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: November 2, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.