Reviewed, UniProtKB/Swiss-Prot P04519 (GSTA_BPT4)

Names and origin

Protein names

Recommended name:
    DNA alpha-glucosyltransferase
      Short name=Alpha-GT
      Short name=AGT
    EC=2.4.1.26

Gene names

Name:

agt

Organism

Enterobacteria phage T4 (Bacteriophage T4)

Taxonomic identifier

10665 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Myoviridae › T4-like viruses

Virus host

Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Is involved in a DNA modification process to protect the phage genome against its own nucleases and the host restriction endonuclease system.
Catalytic activity	Transfers an alpha-D-glucosyl residue from UDP-glucose to an hydroxymethylcytosine residue in DNA.
Pathway	Genetic information processing; DNA modification.

Ontologies

Keywords
Molecular function	Glycosyltransferase Transferase
Technical term	3D-structure
Gene Ontology (GO)
Molecular function	DNA alpha-glucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 400

400

DNA alpha-glucosyltransferase

PRO_0000164941

Secondary structure

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400

Helix Strand Turn

Details...

Beta strand

2 – 7

6

Helix

15 – 29

15

Beta strand

33 – 39

7

Turn

45 – 48

4

Beta strand

49 – 51

3

Turn

53 – 55

3

Beta strand

57 – 59

3

Turn

61 – 63

3

Helix

65 – 72

8

Beta strand

76 – 82

7

Helix

90 – 102

13

Beta strand

107 – 113

7

Helix

118 – 121

4

Beta strand

124 – 126

3

Helix

127 – 133

7

Beta strand

135 – 140

6

Helix

145 – 148

4

Helix

150 – 154

5

Beta strand

161 – 163

3

Beta strand

171 – 173

3

Helix

180 – 187

8

Helix

191 – 193

3

Beta strand

195 – 202

8

Helix

207 – 209

3

Helix

211 – 220

10

Turn

221 – 226

6

Beta strand

228 – 232

5

Helix

238 – 245

8

Beta strand

250 – 253

4

Helix

255 – 260

6

Beta strand

264 – 266

3

Beta strand

269 – 273

5

Helix

277 – 285

9

Beta strand

287 – 292

6

Helix

298 – 300

3

Helix

307 – 315

9

Beta strand

317 – 322

6

Helix

323 – 328

6

Turn

332 – 334

3

Helix

338 – 340

3

Beta strand

346 – 348

3

Helix

353 – 364

12

Helix

367 – 385

19

Helix

387 – 398

12

Sequences

	Sequence		Length	Mass (Da)
	P04519-1 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: 09CC1D4800F5AD2E	FASTA	400	46,704
	10 20 30 40 50 60 MRICIFMARG LEGCGVTKFS LEQRDWFIKN GHEVTLVYAK DKSFTRTSSH DHKSFSIPVI 70 80 90 100 110 120 LAKEYDKALK LVNDCDILII NSVPATSVQE ATINNYKKLL DNIKPSIRVV VYQHDHSVLS 130 140 150 160 170 180 LRRNLGLEET VRRADVIFSH SDNGDFNKVL MKEWYPETVS LFDDIEEAPT VYNFQPPMDI 190 200 210 220 230 240 VKVRSTYWKD VSEINMNINR WIGRTTTWKG FYQMFDFHEK FLKPAGKSTV MEGLERSPAF 250 260 270 280 290 300 IAIKEKGIPY EYYGNREIDK MNLAPNQPAQ ILDCYINSEM LERMSKSGFG YQLSKLNQKY 310 320 330 340 350 360 LQRSLEYTHL ELGACGTIPV FWKSTGENLK FRVDNTPLTS HDSGIIWFDE NDMESTFERI 370 380 390 400 KELSSDRALY DREREKAYEF LYQHQDSSFC FKEQFDIITK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genes 55, alpha gt, 47 and 46 of bacteriophage T4: the genomic organization as deduced by sequence analysis." Gram H., Rueger W. EMBO J. 4:257-264(1985) [PubMed: 4018026] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Bacteriophage T4 genome." Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W. Microbiol. Mol. Biol. Rev. 67:86-156(2003) [PubMed: 12626685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Structural evidence of a passive base-flipping mechanism for AGT, an unusual GT-B glycosyltransferase." Lariviere L., Sommer N., Morera S. J. Mol. Biol. 352:139-150(2005) [PubMed: 16081100] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.73 ANGSTROMS) IN COMPLEX WITH SUBSTRATES.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X01804 Genomic DNA. Translation: CAA25940.1.
M10160 Genomic DNA. Translation: AAC05390.1.
AF158101 Genomic DNA. Translation: AAD42527.1.

PIR

XUBPA4. A00577.

RefSeq

NP_049673.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1XV5	X-ray	1.73	A	1-400	[»]
1Y6F	X-ray	2.40	A/B	1-400	[»]
1Y6G	X-ray	2.80	A/B	1-400	[»]
1Y8Z	X-ray	1.90	A/B	1-400	[»]
1YA6	X-ray	2.40	A/B	1-400	[»]

ModBase

Search...

Protein family/group databases

CAZy

GT72. Glycosyltransferase Family 72.

Genome annotation databases

GeneID

1258823.

GenomeReviews

Gene locus T4p060 in contig AF158101_GR.

Enzyme and pathway databases

BRENDA

2.4.1.26. 1108.

Family and domain databases

InterPro

IPR016223. DNA_alpha-glucosyltransferase.
[Graphical view]

PIRSF

PIRSF000471. DNA_alpha-glucosyltransferase. 1 hit.

ProtoNet

Search...

Entry information

Entry name

GSTA_BPT4

Accession

Primary (citable) accession number: P04519

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	April 14, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)