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P04516 (NSP5_ROTHW) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Non-structural protein 5

Short name=NSP5
Alternative name(s):
NS26
OrganismRotavirus A (strain Human/United States/Wa/1974 G1-P1A[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A) [Complete proteome]
Taxonomic identifier10962 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length197 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in genome replication. Plays a crucial role, together with NSP2, in the formation of virus factories (viroplasms) which are large inclusions in the cytoplasm where core-like replication intermediates are assembled and RNA replication takes place. May regulate NSP2-RNA interactions during genome replication, since NSP5 competes with RNA for the same binding site on the NSP2 octamer. Binds to either ssRNA or dsRNA with similar affinities. Displays ATPase and autokinase activities By similarity.

Cofactor

Magnesium for ATPase activity By similarity.

Subunit structure

Homodimer. Interacts with VP1. Interacts with VP2. Interacts with NSP2; this interaction leads to up-regulation of NSP5 hyperphosphorylation and formation of virus factories. Interacts with NSP6 By similarity.

Subcellular location

Host cytoplasm. Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging By similarity.

Post-translational modification

O-glycosylated By similarity.

Hyperphosphorylated on serine residues, when in dimeric form. Ser-67 phosphorylation by CK1 is required for the hyperphosphorylation of NSP5 dimer. Impaired phosphorylation is associated with a profound morphological change in virus factories and a moderate decrease in virus replication By similarity.

Sequence similarities

Belongs to the rotavirus A NSP5 family.

Sequence caution

The sequence CAA24511.1 differs from that shown. Reason: Frameshift at position 123.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 197197Non-structural protein 5
PRO_0000149637

Regions

Region1 – 4848Interaction with VP1 By similarity
Region188 – 19710Homodimerization and interaction with NSP6 By similarity
Compositional bias2 – 101100Ser-rich

Sites

Metal binding921Magnesium Potential

Amino acid modifications

Modified residue671Phosphoserine; by host CK1 By similarity
Modified residue1531Phosphoserine; by host By similarity
Modified residue1631Phosphoserine; by host By similarity
Modified residue1651Phosphoserine; by host By similarity

Experimental info

Sequence conflict821K → E in CAA24511. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P04516 [UniParc].

Last modified April 14, 2009. Version 2.
Checksum: 5B0C1F736614E864

FASTA19721,670
        10         20         30         40         50         60 
MSLSIDVTSL PSISSSIFKN ESSSTTSTLS GKSIGRNEQY VSSDIEAFNK YMLSKSPEDI 

        70         80         90        100        110        120 
GPSDSASNNP LTSFSIRSNA VKTNADAGVS MDSSTQSRPS SNVGCDQMDF SLTKGINVSA 

       130        140        150        160        170        180 
SLDSCVSIST NHKKEKSKKD KSRKHYPRIE ADSDYEDYVL DDSDSDDGKC KNCKYKKKYF 

       190 
ALRMRMKQVA MQLIEDL 

« Hide

References

[1]"Molecular cloning of double-stranded RNA virus genomes."
Imai M., Richardson M.A., Ikegami N., Shatkin A.J., Furuichi Y.
Proc. Natl. Acad. Sci. U.S.A. 80:373-377(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Nucleotide sequence analysis of rotavirus gene 11 from two tissue culture-adapted ATCC strains, RRV and Wa."
Mohan K.V.K., Atreya C.D.
Virus Genes 23:321-329(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]"Isolation and molecular characterization of a naturally occurring non-structural protein 5 (NSP5) gene reassortant of group A rotavirus of serotype G2P[4] with a long RNA pattern."
Ahmed K., Nakagomi T., Nakagomi O.
J. Med. Virol. 77:323-330(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 2-197.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
V01191 Genomic RNA. Translation: CAA24511.1. Frameshift.
AF306494 Genomic RNA. Translation: AAK15269.1.
AB091726 Genomic RNA. Translation: BAC65999.1.
PIRVHXREH. A04147.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002512. Rotavirus_A/C_NSP5.
[Graphical view]
PfamPF01525. Rota_NS26. 1 hit.
[Graphical view]
PIRSFPIRSF004006. Rota_NS26. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNSP5_ROTHW
AccessionPrimary (citable) accession number: P04516
Secondary accession number(s): Q80IQ6, Q993T2
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: April 14, 2009
Last modified: April 3, 2013
This is version 54 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families