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P04516

- NSP5_ROTHW

UniProt

P04516 - NSP5_ROTHW

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Protein

Non-structural protein 5

Gene
N/A
Organism
Rotavirus A (strain Human/United States/Wa/1974 G1-P1A[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in genome replication. Plays a crucial role, together with NSP2, in the formation of virus factories (viroplasms) which are large inclusions in the cytoplasm where core-like replication intermediates are assembled and RNA replication takes place. May regulate NSP2-RNA interactions during genome replication, since NSP5 competes with RNA for the same binding site on the NSP2 octamer. Binds to either ssRNA or dsRNA with similar affinities. Displays ATPase and autokinase activities (By similarity).By similarity

Cofactori

Mg2+By similarityNote: Magnesium is required for ATPase activity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi92 – 921MagnesiumSequence Analysis

GO - Molecular functioni

  1. ATPase activity Source: InterPro
  2. magnesium ion binding Source: InterPro
  3. nucleotide binding Source: UniProtKB-KW
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. viral genome replication Source: InterPro
Complete GO annotation...

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 5
Short name:
NSP5
Alternative name(s):
NS26
OrganismiRotavirus A (strain Human/United States/Wa/1974 G1-P1A[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A)
Taxonomic identifieri10962 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000006581: Genome

Subcellular locationi

Host cytoplasm
Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 197197Non-structural protein 5PRO_0000149637Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei67 – 671Phosphoserine; by host CK1By similarity
Modified residuei153 – 1531Phosphoserine; by hostBy similarity
Modified residuei163 – 1631Phosphoserine; by hostBy similarity
Modified residuei165 – 1651Phosphoserine; by hostBy similarity

Post-translational modificationi

O-glycosylated.By similarity
Hyperphosphorylated on serine residues, when in dimeric form. Ser-67 phosphorylation by CK1 is required for the hyperphosphorylation of NSP5 dimer. Impaired phosphorylation is associated with a profound morphological change in virus factories and a moderate decrease in virus replication (By similarity).By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Interactioni

Subunit structurei

Homodimer. Interacts with VP1. Interacts with VP2. Interacts with NSP2; this interaction leads to up-regulation of NSP5 hyperphosphorylation and formation of virus factories. Interacts with NSP6 (By similarity).By similarity

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 4848Interaction with VP1By similarityAdd
BLAST
Regioni188 – 19710Homodimerization and interaction with NSP6By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi2 – 101100Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the rotavirus A NSP5 family.Curated

Family and domain databases

InterProiIPR002512. Rotavirus_A/C_NSP5.
[Graphical view]
PfamiPF01525. Rota_NS26. 1 hit.
[Graphical view]
PIRSFiPIRSF004006. Rota_NS26. 1 hit.

Sequencei

Sequence statusi: Complete.

P04516-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLSIDVTSL PSISSSIFKN ESSSTTSTLS GKSIGRNEQY VSSDIEAFNK
60 70 80 90 100
YMLSKSPEDI GPSDSASNNP LTSFSIRSNA VKTNADAGVS MDSSTQSRPS
110 120 130 140 150
SNVGCDQMDF SLTKGINVSA SLDSCVSIST NHKKEKSKKD KSRKHYPRIE
160 170 180 190
ADSDYEDYVL DDSDSDDGKC KNCKYKKKYF ALRMRMKQVA MQLIEDL
Length:197
Mass (Da):21,670
Last modified:April 14, 2009 - v2
Checksum:i5B0C1F736614E864
GO

Sequence cautioni

The sequence CAA24511.1 differs from that shown. Reason: Frameshift at position 123. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti82 – 821K → E in CAA24511. (PubMed:6300836)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01191 Genomic RNA. Translation: CAA24511.1. Frameshift.
AF306494 Genomic RNA. Translation: AAK15269.1.
AB091726 Genomic RNA. Translation: BAC65999.1.
PIRiA04147. VHXREH.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01191 Genomic RNA. Translation: CAA24511.1 . Frameshift.
AF306494 Genomic RNA. Translation: AAK15269.1 .
AB091726 Genomic RNA. Translation: BAC65999.1 .
PIRi A04147. VHXREH.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR002512. Rotavirus_A/C_NSP5.
[Graphical view ]
Pfami PF01525. Rota_NS26. 1 hit.
[Graphical view ]
PIRSFi PIRSF004006. Rota_NS26. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Nucleotide sequence analysis of rotavirus gene 11 from two tissue culture-adapted ATCC strains, RRV and Wa."
    Mohan K.V.K., Atreya C.D.
    Virus Genes 23:321-329(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "Isolation and molecular characterization of a naturally occurring non-structural protein 5 (NSP5) gene reassortant of group A rotavirus of serotype G2P[4] with a long RNA pattern."
    Ahmed K., Nakagomi T., Nakagomi O.
    J. Med. Virol. 77:323-330(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 2-197.

Entry informationi

Entry nameiNSP5_ROTHW
AccessioniPrimary (citable) accession number: P04516
Secondary accession number(s): Q80IQ6, Q993T2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: April 14, 2009
Last modified: November 26, 2014
This is version 57 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3