P04516 (NSP5_ROTHW) Reviewed, UniProtKB/Swiss-Prot
Last modified April 3, 2013. Version 54. History...
Names and origin
|Protein names||Recommended name:|
Non-structural protein 5
|Organism||Rotavirus A (strain Human/United States/Wa/1974 G1-P1A-I1-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A) [Complete proteome]|
|Taxonomic identifier||10962 [NCBI]|
|Taxonomic lineage||Viruses › dsRNA viruses › Reoviridae › Sedoreovirinae › Rotavirus › Rotavirus A ›|
|Virus host||Homo sapiens (Human) [TaxID: 9606]|
|Sequence length||197 AA.|
|Protein existence||Inferred from homology|
General annotation (Comments)
Involved in genome replication. Plays a crucial role, together with NSP2, in the formation of virus factories (viroplasms) which are large inclusions in the cytoplasm where core-like replication intermediates are assembled and RNA replication takes place. May regulate NSP2-RNA interactions during genome replication, since NSP5 competes with RNA for the same binding site on the NSP2 octamer. Binds to either ssRNA or dsRNA with similar affinities. Displays ATPase and autokinase activities By similarity.
Magnesium for ATPase activity By similarity.
Homodimer. Interacts with VP1. Interacts with VP2. Interacts with NSP2; this interaction leads to up-regulation of NSP5 hyperphosphorylation and formation of virus factories. Interacts with NSP6 By similarity.
Host cytoplasm. Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging By similarity.
O-glycosylated By similarity.
Hyperphosphorylated on serine residues, when in dimeric form. Ser-67 phosphorylation by CK1 is required for the hyperphosphorylation of NSP5 dimer. Impaired phosphorylation is associated with a profound morphological change in virus factories and a moderate decrease in virus replication By similarity.
Belongs to the rotavirus A NSP5 family.
|Cellular component||Host cytoplasm|
|Technical term||Complete proteome|
|Gene Ontology (GO)|
|Biological_process||viral genome replication|
Inferred from electronic annotation. Source: InterPro
|Cellular_component||host cell cytoplasm|
Inferred from electronic annotation. Source: UniProtKB-SubCell
Inferred from electronic annotation. Source: InterProRNA binding
Inferred from electronic annotation. Source: UniProtKB-KWmagnesium ion binding
Inferred from electronic annotation. Source: InterPronucleotide binding
Inferred from electronic annotation. Source: UniProtKB-KW
|Complete GO annotation...|
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||1 – 197||197||Non-structural protein 5||PRO_0000149637|
|Region||1 – 48||48||Interaction with VP1 By similarity|
|Region||188 – 197||10||Homodimerization and interaction with NSP6 By similarity|
|Compositional bias||2 – 101||100||Ser-rich|
|Metal binding||92||1||Magnesium Potential|
Amino acid modifications
|Modified residue||67||1||Phosphoserine; by host CK1 By similarity|
|Modified residue||153||1||Phosphoserine; by host By similarity|
|Modified residue||163||1||Phosphoserine; by host By similarity|
|Modified residue||165||1||Phosphoserine; by host By similarity|
|Sequence conflict||82||1||K → E in CAA24511. Ref.1|
|||"Molecular cloning of double-stranded RNA virus genomes."|
Imai M., Richardson M.A., Ikegami N., Shatkin A.J., Furuichi Y.
Proc. Natl. Acad. Sci. U.S.A. 80:373-377(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
|||"Nucleotide sequence analysis of rotavirus gene 11 from two tissue culture-adapted ATCC strains, RRV and Wa."|
Mohan K.V.K., Atreya C.D.
Virus Genes 23:321-329(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
|||"Isolation and molecular characterization of a naturally occurring non-structural protein 5 (NSP5) gene reassortant of group A rotavirus of serotype G2P with a long RNA pattern."|
Ahmed K., Nakagomi T., Nakagomi O.
J. Med. Virol. 77:323-330(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 2-197.
|V01191 Genomic RNA. Translation: CAA24511.1. Frameshift.|
AF306494 Genomic RNA. Translation: AAK15269.1.
AB091726 Genomic RNA. Translation: BAC65999.1.
|PIR||VHXREH. A04147. |
3D structure databases
Protocols and materials databases
Family and domain databases
|InterPro||IPR002512. Rotavirus_A/C_NSP5. |
|Pfam||PF01525. Rota_NS26. 1 hit. |
|PIRSF||PIRSF004006. Rota_NS26. 1 hit. |
|Accession||Primary (citable) accession number: P04516|
Secondary accession number(s): Q80IQ6, Q993T2
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Viral Protein Annotation Program|
Index of protein domains and families