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P04514 (NSP3_ROTBU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Non-structural protein 3

Short name=NSP3
Alternative name(s):
NCVP4
Non-structural RNA-binding protein 34
Short name=NS34
OrganismRotavirus A (strain Cow/United Kingdom/UK/1975 G6-P7[5]-I2-R2-C2-M2-A3-N2-T7-E2-H3) (RV-A) [Complete proteome]
Taxonomic identifier10934 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostBos taurus (Bovine) [TaxID: 9913]

Protein attributes

Sequence length313 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in translation of viral mRNAs and shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection By similarity.

Subunit structure

Homodimer. Interacts (via the coiled-coil region) with host ZC3H7B (via LD motif). Interacts with host EIF4G1 By similarity.

Sequence similarities

Belongs to the rotavirus A NSP3 family.

Sequence caution

The sequence AAA47317.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 313313Non-structural protein 3
PRO_0000149540

Regions

Region4 – 149146RNA-binding By similarity
Region150 – 20657Dimerization By similarity
Region170 – 23465Interaction with ZC3H7B By similarity
Region208 – 313106Interaction with EIF4G1 By similarity
Coiled coil166 – 23772 Potential

Sequences

Sequence LengthMass (Da)Tools
P04514 [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: D6A044D66F27A02E

FASTA31336,153
        10         20         30         40         50         60 
MLKMESTQQM ASSIINTSFE AAVVAATSTL ELMGIQYDYN EIYTRVKSKF DYVMDDSGVK 

        70         80         90        100        110        120 
NNLLGKAATI DQALNGKFGS VMRNKNWMTD SRTVAKLDED VNKLRMMLSS KGIDQKMRVL 

       130        140        150        160        170        180 
NACFSVKRIP GKSSSVIKCT RLMKDKIERG AVEVDDSFVE EKMEVDTVDW KSRYDQLERR 

       190        200        210        220        230        240 
FESLKQRVNE KYTTWVQKAK KVNENMYSLQ NVISQQQNQI ADLQNYCSKL EADLQNKVGS 

       250        260        270        280        290        300 
LVSSVEWYLK SMELPDEVKT DIEQQLNSID TISPINAIDD LEILIRNLIH DYDRTFLMFK 

       310 
GLLRQCNYEY AYE 

« Hide

References

[1]"Nucleotide sequence of gene segment 9 encoding a nonstructural protein of UK bovine rotavirus."
Ward C.W., Elleman T.C., Azad A.A., Dyall-Smith M.L.
Virology 134:249-253(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
K02170 Genomic RNA. Translation: AAA47317.1. Different initiation.

3D structure databases

ProteinModelPortalP04514.
SMRP04514. Positions 7-163, 206-312.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002873. Rotavirus_NSP3.
[Graphical view]
PfamPF01665. Rota_NSP3. 1 hit.
[Graphical view]
SUPFAMSSF69903. SSF69903. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNSP3_ROTBU
AccessionPrimary (citable) accession number: P04514
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: July 9, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families