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Protein

Non-structural protein 3

Gene
N/A
Organism
Rotavirus A (strain RVA/Cow/United Kingdom/UK/1975/G6P7[5]) (RV-A)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Plays an important role in stimulating the translation of viral mRNAs. These mRNAs are capped but not polyadenylated, instead terminating in a conserved sequence 'GACC' at the 3' that is recognized by NSP3, which competes with host PABPC1 for EIF4G1 binding. The interaction between NSP3 and host EIF4G1 stabilizes the EIF4E-EIF4G1 interaction, thereby facilitating the initiation of capped mRNA translation.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processHost-virus interaction, Translation regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 3UniRule annotation
Short name:
NSP3UniRule annotation
Alternative name(s):
NCVP4UniRule annotation
Non-structural RNA-binding protein 34UniRule annotation
Short name:
NS34UniRule annotation
OrganismiRotavirus A (strain RVA/Cow/United Kingdom/UK/1975/G6P7[5]) (RV-A)
Taxonomic identifieri10934 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiBos taurus (Bovine) [TaxID: 9913]
Proteomesi
  • UP000008657 Componenti: Genome

Subcellular locationi

  • Host cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001495401 – 313Non-structural protein 3Add BLAST313

Interactioni

Subunit structurei

Homodimer. Interacts (via the coiled-coil region) with host ZC3H7B (via LD motif). Interacts with host EIF4G1.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP04514
SMRiP04514
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 149RNA-bindingUniRule annotationAdd BLAST149
Regioni150 – 206DimerizationUniRule annotationAdd BLAST57
Regioni170 – 234Interaction with host ZC3H7BUniRule annotationAdd BLAST65
Regioni208 – 313Interaction with host EIF4G1UniRule annotationAdd BLAST106

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili166 – 237UniRule annotationAdd BLAST72

Sequence similaritiesi

Belongs to the rotavirus NSP3 family.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

OrthoDBiVOG090000G7

Family and domain databases

HAMAPiMF_04094 ROTA_A_NSP3, 1 hit
MF_04090 ROTA_NSP3, 1 hit
InterProiView protein in InterPro
IPR036082 NSP3_sf
IPR002873 Rotavirus_NSP3
PfamiView protein in Pfam
PF01665 Rota_NSP3, 1 hit
SUPFAMiSSF69903 SSF69903, 1 hit

Sequencei

Sequence statusi: Complete.

P04514-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKMESTQQM ASSIINTSFE AAVVAATSTL ELMGIQYDYN EIYTRVKSKF
60 70 80 90 100
DYVMDDSGVK NNLLGKAATI DQALNGKFGS VMRNKNWMTD SRTVAKLDED
110 120 130 140 150
VNKLRMMLSS KGIDQKMRVL NACFSVKRIP GKSSSVIKCT RLMKDKIERG
160 170 180 190 200
AVEVDDSFVE EKMEVDTVDW KSRYDQLERR FESLKQRVNE KYTTWVQKAK
210 220 230 240 250
KVNENMYSLQ NVISQQQNQI ADLQNYCSKL EADLQNKVGS LVSSVEWYLK
260 270 280 290 300
SMELPDEVKT DIEQQLNSID TISPINAIDD LEILIRNLIH DYDRTFLMFK
310
GLLRQCNYEY AYE
Length:313
Mass (Da):36,153
Last modified:August 13, 1987 - v1
Checksum:iD6A044D66F27A02E
GO

Sequence cautioni

The sequence AAA47317 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02170 Genomic RNA Translation: AAA47317.1 Different initiation.

Similar proteinsi

Entry informationi

Entry nameiNSP3_ROTBU
AccessioniPrimary (citable) accession number: P04514
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: April 25, 2018
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health