P04507 (SIGM1_REOVJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Outer capsid protein sigma-1
Short name=Sigma1

Alternative name(s):
Cell attachment protein
Hemagglutinin

Gene names

Name:

S1

Organism

Reovirus type 2 (strain D5/Jones) (T2J) (Mammalian orthoreovirus 2) [Complete proteome]

Taxonomic identifier

10885 [NCBI]

Taxonomic lineage

Viruses › dsRNA viruses › Reoviridae › Spinareovirinae › Orthoreovirus › Mammalian orthoreovirus › Mammalian orthoreovirus 2

Virus host

Mammalia [TaxID: 40674]

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Fiber-like molecule that attaches the virion to the host cell membrane by binding to the primary receptor F11R/JAM-A and to sialic acid containing proteins (coreceptor). The interaction of sigma-1 with F11R is required for NF-kB activation and apoptosis. Binding to both sialic acid and F11R is required to induce maximal levels of apoptosis By similarity.
Subunit structure	Homotrimer. Interacts (via the head region) with human F11R By similarity.
Subcellular location	Virion By similarity. Note: Found in the outer capsid (36 copies) By similarity.
Post-translational modification	Undergoes dramatic conformational rearrangements during viral disassembly in the endocytic pathway By similarity.
Sequence similarities	Belongs to the orthoreovirus sigma-1 protein family.
Sequence caution	The sequence AAA66879.1 differs from that shown. Reason: Frameshift at positions 370 and 415.

Ontologies

Keywords
Biological process	Host-virus interaction Viral attachment to host cell Virus entry into host cell
Cellular component	Virion
Domain	Coiled coil
Molecular function	Capsid protein Hemagglutinin
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from electronic annotation. Source: InterPro viral attachment to host cell Inferred from electronic annotation. Source: UniProtKB-KW viral entry into host cell Inferred from electronic annotation. Source: UniProtKB-KW viral infectious cycle Inferred from electronic annotation. Source: InterPro
Cellular_component	viral capsid Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 462	462	Outer capsid protein sigma-1		PRO_0000040666
Regions
	Region	1 – 317	317	Tail
	Region	318 – 462	145	Head
	Coiled coil	18 – 49	32	Potential
Amino acid modifications
	Glycosylation	237	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	243	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	297	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	413	1	N-linked (GlcNAc...); by host Potential
Experimental info
	Sequence conflict	147	1	V → L in AAA47268. Ref.2
	Sequence conflict	207	1	L → V in AAA47251. Ref.3
	Sequence conflict	439	1	H → Y in AAA47251. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P04507 [UniParc]. Last modified July 22, 2008. Version 3. Checksum: 87383752835EEA8F	FASTA	462	50,466
	10 20 30 40 50 60 MSDLVQLIRR EILLLTGNGE SANSKHEIEE IKKQIKDISA DVNRISNIVD SIQGQLGGLS 70 80 90 100 110 120 VRVSAIESGV SENGNRIDRL ERDVSGISAS VSGIDSRLSE LGDRVNVAEQ RIGQLDTVTD 130 140 150 160 170 180 NLLERASRLE TEVSAITNDL GSLNTRVTTE LNDVRQTIAA IDTRLTTLET DAVTSVGQGL 190 200 210 220 230 240 QKTGNSIKVI VGTGMWFDRN NVLQLFLSNQ QKGLGFIDNG MVVKIDTQYF SFDSNGNITL 250 260 270 280 290 300 NNNISGLPAR TGSLEASRID VVAPPLVIQS TGSTRLLRLM YEAVDFVVTN NVLTLRNRSV 310 320 330 340 350 360 TPTFKFPLEL NSADNSVSIH RNYRIRLGQW SGQLEYHTPS LRWNAPVTVN LMRVDDWLIL 370 380 390 400 410 420 SFTRFSTSGI LASGKFVLNF VTGLSPGWAT GSTEPSTTTN PLSTTFAAIQ FINGSSRVDA 430 440 450 460 FRILGVAEWN AGELEITNHG GTYTAHTNVD WAPMTIMYPC LG

References

[1]	"Sequences of the S1 genes of the three serotypes of reovirus." Cashdollar L.W., Chmelo R.A., Wiener J.R., Joklik W.K. Proc. Natl. Acad. Sci. U.S.A. 82:24-28(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Identification of conserved domains in the cell attachment proteins of the three serotypes of reovirus." Duncan R., Horne D., Cashdollar L.W., Joklik W.K., Lee P.W.K. Virology 174:399-409(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]	"Structure of the reovirus cell-attachment protein: a model for the domain organization of sigma 1." Nibert M.L., Dermody T.S., Fields B.N. J. Virol. 64:2976-2989(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M10261 Genomic RNA. Translation: AAA66879.1. Frameshift. M32861 Genomic RNA. Translation: AAA47268.1. M35964 Genomic RNA. Translation: AAA47251.1.
PIR	HMXRH2. A04123. C34829.
3D structure databases
ProteinModelPortal	P04507.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.60.90.20. 1 hit.
InterPro	IPR008982. Adenovirus_pIV-rel_att. IPR027314. Sigma1_globular_dom. IPR002592. Vir_attach_sigma1_reovir. [Graphical view]
Pfam	PF01664. Reo_sigma1. 1 hit. [Graphical view]
SUPFAM	SSF49835. Viral_att. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SIGM1_REOVJ

Accession

Primary (citable) accession number: P04507
Secondary accession number(s): Q85663

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	July 22, 2008
Last modified:	May 1, 2013
This is version 80 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program