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P04506

- SIGM1_REOVL

UniProt

P04506 - SIGM1_REOVL

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Protein

Outer capsid protein sigma-1

Gene

S1

Organism
Reovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Fiber-like molecule that attaches the virion to the host cell membrane by binding to the primary receptor F11R/JAM-A and to sialic acid containing proteins (coreceptor). The interaction of sigma-1 with F11R is required for NF-kB activation and apoptosis. Binding to both sialic acid and F11R is required to induce maximal levels of apoptosis (By similarity).By similarity

GO - Biological processi

  1. cell adhesion Source: InterPro
  2. viral entry into host cell Source: UniProtKB-KW
  3. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin

Keywords - Biological processi

Host-virus interaction, Viral attachment to host cell, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Outer capsid protein sigma-1
Short name:
Sigma1
Alternative name(s):
Cell attachment protein
Hemagglutinin
Gene namesi
Name:S1
OrganismiReovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1)
Taxonomic identifieri10884 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
Virus hostiMammalia [TaxID: 40674]
ProteomesiUP000007253: Genome

Subcellular locationi

Virion By similarity
Note: Found in the outer capsid (36 copies).By similarity

GO - Cellular componenti

  1. viral capsid Source: CACAO
  2. viral outer capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Outer capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 470470Outer capsid protein sigma-1PRO_0000040667Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi21 – 211N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi121 – 1211N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi205 – 2051N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi353 – 3531N-linked (GlcNAc...); by hostSequence Analysis

Post-translational modificationi

Undergoes dramatic conformational rearrangements during viral disassembly in the endocytic pathway.By similarity

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Homotrimer. Interacts (via the head region) with human F11R (By similarity).By similarity

Structurei

Secondary structure

1
470
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi274 – 2785Combined sources
Beta strandi281 – 2855Combined sources
Turni290 – 2923Combined sources
Beta strandi293 – 2964Combined sources
Beta strandi299 – 3046Combined sources
Beta strandi315 – 3184Combined sources
Turni319 – 3224Combined sources
Beta strandi323 – 3264Combined sources
Helixi328 – 3314Combined sources
Beta strandi333 – 34513Combined sources
Beta strandi348 – 36114Combined sources
Beta strandi364 – 3696Combined sources
Beta strandi372 – 3754Combined sources
Beta strandi379 – 3868Combined sources
Helixi397 – 4004Combined sources
Beta strandi409 – 41911Combined sources
Beta strandi421 – 43717Combined sources
Beta strandi440 – 4478Combined sources
Beta strandi454 – 4585Combined sources
Beta strandi461 – 4666Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GU3X-ray3.60A/B/C261-470[»]
4GU4X-ray3.50A/B/C261-470[»]
ProteinModelPortaliP04506.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 324324TailAdd
BLAST
Regioni325 – 470146HeadAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili26 – 4621Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Family and domain databases

Gene3Di2.10.25.20. 1 hit.
2.60.90.20. 1 hit.
InterProiIPR008982. Adenovirus_pIV-rel_att.
IPR009013. Attachment_protein_shaft_dom.
IPR027314. Sigma1_globular_dom.
IPR002592. Vir_attach_sigma1_reovir.
[Graphical view]
PfamiPF01664. Reo_sigma1. 1 hit.
[Graphical view]
SUPFAMiSSF49835. SSF49835. 1 hit.

Sequencei

Sequence statusi: Complete.

P04506-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDASLITEIR KIVLQLSVSS NGSQSKEIEE IKKQVQVNVD DIRAANIKLD
60 70 80 90 100
GLGRQIADIS NSISTIESRL GEMDNRLVGI SSQVTQLSNS VSQNTQSISS
110 120 130 140 150
LGDRINAVEP RVDSLDTVTS NLTGRTSTLE ADVGSLRTEL AALTTRVTTE
160 170 180 190 200
VTRLDGLINS GQNSIGELST RLSNVETSMV TTAGRGLQKN GNTLNVIVGN
210 220 230 240 250
GMWFNSSNQL QLDLSGQSKG VGFVGTGMVV KIDTNYFAYN SNGEITLVSQ
260 270 280 290 300
INELPSRVST LESAKIDSVL PPLTVREASG VRTLSFGYDT SDFTIINSVL
310 320 330 340 350
SLRSRLTLPT YRYPLELDTA NNRVQVADRF GMRTGTWTGQ LQYQHPQLSW
360 370 380 390 400
RANVTLNLMK VDDWLVLSFS QMTTNSIMAD GKFVINFVSG LSSGWQTGDT
410 420 430 440 450
EPSSTIDPLS TTFAAVQFLN NGQRIDAFRI MGVSEWTDGE LEIKNYGGTY
460 470
TGHTQVYWAP WTIMYPCNVR
Length:470
Mass (Da):51,404
Last modified:November 1, 1990 - v2
Checksum:iF4D18989AD54491C
GO

Sequence cautioni

The sequence AAA47276.1 differs from that shown. Reason: Frameshift at position 410. Curated
The sequence AAA66877.1 differs from that shown. Reason: Frameshift at positions 410 and 434. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti91 – 911V → G in AAA66877. (PubMed:3855545)Curated
Sequence conflicti91 – 911V → G in AAA47276. (PubMed:2430568)Curated
Sequence conflicti169 – 1691S → F in AAA66877. (PubMed:3855545)Curated
Sequence conflicti384 – 3841V → L in AAA47276. (PubMed:2430568)Curated
Sequence conflicti409 – 4091L → W in AAA47242. (PubMed:2335823)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10260 Genomic RNA. Translation: AAA66877.1. Frameshift.
M14779 Genomic RNA. Translation: AAA47276.1. Frameshift.
M32860 Genomic RNA. Translation: AAA47267.1.
M35963 Genomic RNA. Translation: AAA47242.1.
EF494445 Genomic RNA. Translation: ABP48923.1.
AH002406 Genomic RNA. Translation: AAA47240.1.
PIRiA04122. HMXRH1.
A34829. HMXRL1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10260 Genomic RNA. Translation: AAA66877.1 . Frameshift.
M14779 Genomic RNA. Translation: AAA47276.1 . Frameshift.
M32860 Genomic RNA. Translation: AAA47267.1 .
M35963 Genomic RNA. Translation: AAA47242.1 .
EF494445 Genomic RNA. Translation: ABP48923.1 .
AH002406 Genomic RNA. Translation: AAA47240.1 .
PIRi A04122. HMXRH1.
A34829. HMXRL1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4GU3 X-ray 3.60 A/B/C 261-470 [» ]
4GU4 X-ray 3.50 A/B/C 261-470 [» ]
ProteinModelPortali P04506.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.10.25.20. 1 hit.
2.60.90.20. 1 hit.
InterProi IPR008982. Adenovirus_pIV-rel_att.
IPR009013. Attachment_protein_shaft_dom.
IPR027314. Sigma1_globular_dom.
IPR002592. Vir_attach_sigma1_reovir.
[Graphical view ]
Pfami PF01664. Reo_sigma1. 1 hit.
[Graphical view ]
SUPFAMi SSF49835. SSF49835. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Sequences of the S1 genes of the three serotypes of reovirus."
    Cashdollar L.W., Chmelo R.A., Wiener J.R., Joklik W.K.
    Proc. Natl. Acad. Sci. U.S.A. 82:24-28(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Biosynthesis of reovirus-specified polypeptides. Molecular cDNA cloning and nucleotide sequence of the reovirus serotype 1 Lang strain bicistronic s1 mRNA which encodes the minor capsid polypeptide sigma 1a and the nonstructural polypeptide sigma 1bNS."
    Munemitsu S.M., Atwater J.A., Samuel C.E.
    Biochem. Biophys. Res. Commun. 140:508-514(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "Identification of conserved domains in the cell attachment proteins of the three serotypes of reovirus."
    Duncan R., Horne D., Cashdollar L.W., Joklik W.K., Lee P.W.K.
    Virology 174:399-409(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  4. "Structure of the reovirus cell-attachment protein: a model for the domain organization of sigma 1."
    Nibert M.L., Dermody T.S., Fields B.N.
    J. Virol. 64:2976-2989(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Infectious clone.
  6. "Nature of the 3'-terminal sequences of the plus and minus strands of the S1 gene of reovirus serotypes 1, 2 and 3."
    Li J.K.-K., Keene J.D., Scheible P.P., Joklik W.K.
    Virology 105:41-51(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-21.

Entry informationi

Entry nameiSIGM1_REOVL
AccessioniPrimary (citable) accession number: P04506
Secondary accession number(s): A4ZY30, P07937
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: November 1, 1990
Last modified: November 26, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3