P04505 (GP_BUNYW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Envelope glycoprotein
Short name=GP

Alternative name(s):
M polyprotein

Cleaved into the following 3 chains:

1. Glycoprotein G2
   Short name=Gn
2. Non-structural protein NSm
3. Glycoprotein G1
   Short name=Gc

Gene names

Name:

GP

Organism

Bunyamwera virus (BUNV) [Reference proteome]

Taxonomic identifier

35304 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Bunyaviridae › Orthobunyavirus

Virus host

Aedes aegypti (Yellowfever mosquito) (Culex aegypti) [TaxID: 7159]
Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	1433 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Glycoprotein G1 and glycoprotein G2 interact with each other and are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion By similarity.
Subunit structure	G1 and G2 interacts with each other By similarity.
Subcellular location	Glycoprotein G1: Virion membrane; Single-pass type I membrane protein Potential. Host Golgi apparatus membrane; Single-pass type I membrane protein Potential. Host endoplasmic reticulum membrane; Single-pass type I membrane protein Potential. Note: G1 alone is retained in the membrane of the endoplasmic reticulum, but not transported to the Golgi. Coexpression of G1 and G2 results in efficient transport of G1 to the Golgi complex, indicating that their interaction is essential for proper targeting to this organelle, where virion budding occurs. Ref.2 Ref.3 Glycoprotein G2: Virion membrane; Single-pass type I membrane protein Potential. Host Golgi apparatus membrane; Single-pass type I membrane protein Potential. Note: G2 is retained in the Golgi complex through a Golgi retention signal, which resides in the G2 transmembrane region. Ref.2 Ref.3 Non-structural protein NSm: Host Golgi apparatus membrane; Multi-pass membrane protein Potential Ref.2 Ref.3.
Post-translational modification	Specific enzymatic cleavages in vivo yield mature proteins including nonstructural protein NSm, glycoprotein G1, and glycoprotein G2.
Sequence similarities	Belongs to the orthobunyavirus envelope glycoprotein family.

Ontologies

Keywords
Biological process	Fusion of virus membrane with host endosomal membrane Fusion of virus membrane with host membrane Host-virus interaction Viral attachment to host cell Viral penetration into host cytoplasm Virus entry into host cell
Cellular component	Host Golgi apparatus Host endoplasmic reticulum Host membrane Membrane Virion
Domain	Signal Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	viral attachment to host cell Inferred from electronic annotation. Source: UniProtKB-KW viral entry into host cell Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell host cell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16
	Chain	17 – 1433	1417	Envelope glycoprotein		PRO_0000036798
	Chain	17 – 302	286	Glycoprotein G2 By similarity		PRO_0000036799
	Chain	303 – 477	175	Non-structural protein NSm By similarity		PRO_0000036800
	Chain	478 – 1433	956	Glycoprotein G1 By similarity		PRO_0000036801
Regions
	Topological domain	17 – 203	187	Lumenal Potential
	Transmembrane	204 – 224	21	Helical; Potential
	Topological domain	225 – 308	84	Cytoplasmic Potential
	Transmembrane	309 – 329	21	Helical; Potential
	Topological domain	330 – 369	40	Lumenal Potential
	Transmembrane	370 – 390	21	Helical; Potential
	Topological domain	391 – 456	66	Cytoplasmic Potential
	Transmembrane	457 – 477	21	Helical; Potential
	Topological domain	478 – 1387	910	Lumenal Potential
	Transmembrane	1388 – 1408	21	Helical; Potential
	Topological domain	1409 – 1433	25	Cytoplasmic Potential
Sites
	Site	477 – 478	2	Cleavage; by host signal peptidase By similarity
Amino acid modifications
	Glycosylation	60	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	624	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	1169	1	N-linked (GlcNAc...); by host Potential

Sequences

	Sequence		Length	Mass (Da)
	P04505 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: CD61ABDE782018E0	FASTA	1,433	162,078
	10 20 30 40 50 60 MRILILLLAV TQLAVSSPVI TRCFHGGQLI AERKSQTSIS EFCIKDDVSM LKSEIVYTKN 70 80 90 100 110 120 DTGIFGHSKV FRHWTITDWK ACNPVVTAGG SINVIEVDKN LNLVTRNYVC TGDCTITVDR 130 140 150 160 170 180 KNAQIIFQTD KLNHFEVTGT TISTGWFKSK ASVTLDRTCE HIKVSCGKKT LQFHACFKQH 190 200 210 220 230 240 MSCVRFLHRS ILPGSMAISI CQNIELIIIT ILALCIFIIM IILTKTYICY VLIPVFMPIA 250 260 270 280 290 300 FAYGWAYNRS CKKCTCCGLA YHPFTNCGSY CVCGSKFETS DRMRMHRESG LCQGFKSLRV 310 320 330 340 350 360 ARRLCKSKGS SLIISILLSV LILSFVTPIE GTLTNYPTDQ KYTLDEIADV LQAKTHEDST 370 380 390 400 410 420 KYYIILYTSL FGAGLTIIFA GVALGLTIIL EVLTKINVIF CNECNMYHSK KSIKYVGDFT 430 440 450 460 470 480 NKCGFCTCGL LEDPEGVVVH KAKKSCTYSY QINWVRGIMI FVAFLFVIQN TIIMVAAEED 490 500 510 520 530 540 CWKNEELKED CVGPLIAPKD CTDKDHKTYL SEASLLATAK KITQVDAENV EILGKTMESA 550 560 570 580 590 600 IRVIERQKTY HRMHLLEAVF LNKHCDYYKM FEHNSGYSQV KWRMMIKTQH FDICALQANS 610 620 630 640 650 660 PFCAQCIADN SCAQGSWEFD THMNSTYSSK VDNFKHDFSL FLRIFEAAFP GTAYVHLLTN 670 680 690 700 710 720 IKEKKPYQAV SMIEKIKKKF PNNKLLIGYL DFGKYLLGLS HASTYELQQR QLDKLYQPTE 730 740 750 760 770 780 LTRSGGQQTS LANSVVGQAT KECKKYKDVS CLSPRFGIPL EDLISCCDQP NYNIYKKPKK 790 800 810 820 830 840 VYKAHDKEET WCINDQHCLV DFVPAEADTV EKLKPMKCWL VDPGKNDDVY SIAIKTCRVV 850 860 870 880 890 900 DKGVCTVNSQ KWNIIKCDSG PLYYSDHIPG EDTGNDIGHY CVSAGCKTDR YPINPDVVTD 910 920 930 940 950 960 CVWEFTSRKS QYIGKISMQS LEDYEKALTD RLTHTLETYS FAPLENLPHI KPVYKYITAQ 970 980 990 1000 1010 1020 GVENSDGIEG AFITASIPAA GGTSIGYNVR SKDGFPLLDL IVFVKSAVIK STYNHIYDTG 1030 1040 1050 1060 1070 1080 PTISINTKHD EHCTGQCPSN IEHEANWLTF SQERTSRWGC EEFGCLAVNT GCVFGSCQDV 1090 1100 1110 1120 1130 1140 IRPETKVYRK AVDEVVILTV CITYPGHTFC TEINAIEPKI TEEIELQFKT VDTKTLPYIV 1150 1160 1170 1180 1190 1200 AVNNHKLYSG QINDLGTFGQ MCGNVQKTNS SILGTGTPKF DYTCHGASRK DIIVRRCYNN 1210 1220 1230 1240 1250 1260 NFDSCKLLKE ETQLIFNDDH DTITVYNTNH LIGELAIKLI LGDIQYKLFT ETLDLQIDAK 1270 1280 1290 1300 1310 1320 CVGCPDCFES YSCNFQIVSN IDTICSLEGP CDTFHNRISI KAMQQNYAVK LSCQKDPRPS 1330 1340 1350 1360 1370 1380 GTFKICNREY TVVFHTVAKD DKIEINVGDQ TSFIKEKDDR CKTWLCRVRD EGISVIFEPI 1390 1400 1410 1420 1430 KAFFGSYFSI FFYIIVVVVV GFLIIYIFMP MFMKLKEVLK ANEKLYLQEI KQK

References

[1]	"Nucleotide sequence of the Bunyamwera virus M RNA segment: conservation of structural features in the Bunyavirus glycoprotein gene product." Lees J.F., Pringle C.R., Elliott R.M. Virology 148:1-14(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Localization of Bunyamwera bunyavirus G1 glycoprotein to the Golgi requires association with G2 but not with NSm." Lappin D.F., Nakitare G.W., Palfreyman J.W., Elliott R.M. J. Gen. Virol. 75:3441-3451(1994) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[3]	"Mapping the Golgi targeting and retention signal of Bunyamwera virus glycoproteins." Shi X., Lappin D.F., Elliott R.M. J. Virol. 78:10793-10802(2004) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[4]	"Requirement of the N-terminal region of orthobunyavirus nonstructural protein NSm for virus assembly and morphogenesis." Shi X., Kohl A., Leonard V.H., Li P., McLees A., Elliott R.M. J. Virol. 80:8089-8099(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M11852 Genomic RNA. Translation: AAA42777.1.
PIR	GNVUBW. A04101.
RefSeq	NP_047212.1. NC_001926.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-404N.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2648216.
Family and domain databases
InterPro	IPR005167. Bunya_G1. IPR005168. Bunya_G2. IPR026400. Bunya_nonstruc_pro_NSm. IPR014413. M_poly_OrthobunV. [Graphical view]
Pfam	PF03557. Bunya_G1. 1 hit. PF03563. Bunya_G2. 1 hit. [Graphical view]
PIRSF	PIRSF003944. M_poly_OrthobunV. 1 hit.
TIGRFAMs	TIGR04210. bunya_NSm. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GP_BUNYW

Accession

Primary (citable) accession number: P04505

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	April 3, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program