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P04505 (GP_BUNYW) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Envelope glycoprotein

Short name=GP
Alternative name(s):
M polyprotein

Cleaved into the following 3 chains:

  1. Glycoprotein G2
    Short name=Gn
  2. Non-structural protein NSm
  3. Glycoprotein G1
    Short name=Gc
Gene names
Name:GP
OrganismBunyamwera virus (BUNV) [Reference proteome]
Taxonomic identifier35304 [NCBI]
Taxonomic lineageVirusesssRNA negative-strand virusesBunyaviridaeOrthobunyavirus
Virus hostAedes aegypti (Yellowfever mosquito) (Culex aegypti) [TaxID: 7159]
Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length1433 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Glycoprotein G1 and glycoprotein G2 interact with each other and are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion By similarity.

Subunit structure

G1 and G2 interacts with each other By similarity.

Subcellular location

Glycoprotein G1: Virion membrane; Single-pass type I membrane protein Potential. Host Golgi apparatus membrane; Single-pass type I membrane protein Potential. Host endoplasmic reticulum membrane; Single-pass type I membrane protein Potential. Note: G1 alone is retained in the membrane of the endoplasmic reticulum, but not transported to the Golgi. Coexpression of G1 and G2 results in efficient transport of G1 to the Golgi complex, indicating that their interaction is essential for proper targeting to this organelle, where virion budding occurs. Ref.2 Ref.3

Glycoprotein G2: Virion membrane; Single-pass type I membrane protein Potential. Host Golgi apparatus membrane; Single-pass type I membrane protein Potential. Note: G2 is retained in the Golgi complex through a Golgi retention signal, which resides in the G2 transmembrane region. Ref.2 Ref.3

Non-structural protein NSm: Host Golgi apparatus membrane; Multi-pass membrane protein Potential Ref.2 Ref.3.

Post-translational modification

Specific enzymatic cleavages in vivo yield mature proteins including nonstructural protein NSm, glycoprotein G1, and glycoprotein G2.

Sequence similarities

Belongs to the orthobunyavirus envelope glycoprotein family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1616
Chain17 – 14331417Envelope glycoprotein
PRO_0000036798
Chain17 – 302286Glycoprotein G2 By similarity
PRO_0000036799
Chain303 – 477175Non-structural protein NSm By similarity
PRO_0000036800
Chain478 – 1433956Glycoprotein G1 By similarity
PRO_0000036801

Regions

Topological domain17 – 203187Lumenal Potential
Transmembrane204 – 22421Helical; Potential
Topological domain225 – 30884Cytoplasmic Potential
Transmembrane309 – 32921Helical; Potential
Topological domain330 – 36940Lumenal Potential
Transmembrane370 – 39021Helical; Potential
Topological domain391 – 45666Cytoplasmic Potential
Transmembrane457 – 47721Helical; Potential
Topological domain478 – 1387910Lumenal Potential
Transmembrane1388 – 140821Helical; Potential
Topological domain1409 – 143325Cytoplasmic Potential

Sites

Site477 – 4782Cleavage; by host signal peptidase By similarity

Amino acid modifications

Glycosylation601N-linked (GlcNAc...); by host Potential
Glycosylation6241N-linked (GlcNAc...); by host Potential
Glycosylation11691N-linked (GlcNAc...); by host Potential

Sequences

Sequence LengthMass (Da)Tools
P04505 [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: CD61ABDE782018E0

FASTA1,433162,078
        10         20         30         40         50         60 
MRILILLLAV TQLAVSSPVI TRCFHGGQLI AERKSQTSIS EFCIKDDVSM LKSEIVYTKN 

        70         80         90        100        110        120 
DTGIFGHSKV FRHWTITDWK ACNPVVTAGG SINVIEVDKN LNLVTRNYVC TGDCTITVDR 

       130        140        150        160        170        180 
KNAQIIFQTD KLNHFEVTGT TISTGWFKSK ASVTLDRTCE HIKVSCGKKT LQFHACFKQH 

       190        200        210        220        230        240 
MSCVRFLHRS ILPGSMAISI CQNIELIIIT ILALCIFIIM IILTKTYICY VLIPVFMPIA 

       250        260        270        280        290        300 
FAYGWAYNRS CKKCTCCGLA YHPFTNCGSY CVCGSKFETS DRMRMHRESG LCQGFKSLRV 

       310        320        330        340        350        360 
ARRLCKSKGS SLIISILLSV LILSFVTPIE GTLTNYPTDQ KYTLDEIADV LQAKTHEDST 

       370        380        390        400        410        420 
KYYIILYTSL FGAGLTIIFA GVALGLTIIL EVLTKINVIF CNECNMYHSK KSIKYVGDFT 

       430        440        450        460        470        480 
NKCGFCTCGL LEDPEGVVVH KAKKSCTYSY QINWVRGIMI FVAFLFVIQN TIIMVAAEED 

       490        500        510        520        530        540 
CWKNEELKED CVGPLIAPKD CTDKDHKTYL SEASLLATAK KITQVDAENV EILGKTMESA 

       550        560        570        580        590        600 
IRVIERQKTY HRMHLLEAVF LNKHCDYYKM FEHNSGYSQV KWRMMIKTQH FDICALQANS 

       610        620        630        640        650        660 
PFCAQCIADN SCAQGSWEFD THMNSTYSSK VDNFKHDFSL FLRIFEAAFP GTAYVHLLTN 

       670        680        690        700        710        720 
IKEKKPYQAV SMIEKIKKKF PNNKLLIGYL DFGKYLLGLS HASTYELQQR QLDKLYQPTE 

       730        740        750        760        770        780 
LTRSGGQQTS LANSVVGQAT KECKKYKDVS CLSPRFGIPL EDLISCCDQP NYNIYKKPKK 

       790        800        810        820        830        840 
VYKAHDKEET WCINDQHCLV DFVPAEADTV EKLKPMKCWL VDPGKNDDVY SIAIKTCRVV 

       850        860        870        880        890        900 
DKGVCTVNSQ KWNIIKCDSG PLYYSDHIPG EDTGNDIGHY CVSAGCKTDR YPINPDVVTD 

       910        920        930        940        950        960 
CVWEFTSRKS QYIGKISMQS LEDYEKALTD RLTHTLETYS FAPLENLPHI KPVYKYITAQ 

       970        980        990       1000       1010       1020 
GVENSDGIEG AFITASIPAA GGTSIGYNVR SKDGFPLLDL IVFVKSAVIK STYNHIYDTG 

      1030       1040       1050       1060       1070       1080 
PTISINTKHD EHCTGQCPSN IEHEANWLTF SQERTSRWGC EEFGCLAVNT GCVFGSCQDV 

      1090       1100       1110       1120       1130       1140 
IRPETKVYRK AVDEVVILTV CITYPGHTFC TEINAIEPKI TEEIELQFKT VDTKTLPYIV 

      1150       1160       1170       1180       1190       1200 
AVNNHKLYSG QINDLGTFGQ MCGNVQKTNS SILGTGTPKF DYTCHGASRK DIIVRRCYNN 

      1210       1220       1230       1240       1250       1260 
NFDSCKLLKE ETQLIFNDDH DTITVYNTNH LIGELAIKLI LGDIQYKLFT ETLDLQIDAK 

      1270       1280       1290       1300       1310       1320 
CVGCPDCFES YSCNFQIVSN IDTICSLEGP CDTFHNRISI KAMQQNYAVK LSCQKDPRPS 

      1330       1340       1350       1360       1370       1380 
GTFKICNREY TVVFHTVAKD DKIEINVGDQ TSFIKEKDDR CKTWLCRVRD EGISVIFEPI 

      1390       1400       1410       1420       1430 
KAFFGSYFSI FFYIIVVVVV GFLIIYIFMP MFMKLKEVLK ANEKLYLQEI KQK 

« Hide

References

[1]"Nucleotide sequence of the Bunyamwera virus M RNA segment: conservation of structural features in the Bunyavirus glycoprotein gene product."
Lees J.F., Pringle C.R., Elliott R.M.
Virology 148:1-14(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Localization of Bunyamwera bunyavirus G1 glycoprotein to the Golgi requires association with G2 but not with NSm."
Lappin D.F., Nakitare G.W., Palfreyman J.W., Elliott R.M.
J. Gen. Virol. 75:3441-3451(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[3]"Mapping the Golgi targeting and retention signal of Bunyamwera virus glycoproteins."
Shi X., Lappin D.F., Elliott R.M.
J. Virol. 78:10793-10802(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[4]"Requirement of the N-terminal region of orthobunyavirus nonstructural protein NSm for virus assembly and morphogenesis."
Shi X., Kohl A., Leonard V.H., Li P., McLees A., Elliott R.M.
J. Virol. 80:8089-8099(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M11852 Genomic RNA. Translation: AAA42777.1.
PIRGNVUBW. A04101.
RefSeqNP_047212.1. NC_001926.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-404N.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2648216.

Family and domain databases

InterProIPR005167. Bunya_G1.
IPR005168. Bunya_G2.
IPR026400. Bunya_nonstruc_pro_NSm.
IPR014413. M_poly_OrthobunV.
[Graphical view]
PfamPF03557. Bunya_G1. 1 hit.
PF03563. Bunya_G2. 1 hit.
[Graphical view]
PIRSFPIRSF003944. M_poly_OrthobunV. 1 hit.
TIGRFAMsTIGR04210. bunya_NSm. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGP_BUNYW
AccessionPrimary (citable) accession number: P04505
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: February 19, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families