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P04505

- GP_BUNYW

UniProt

P04505 - GP_BUNYW

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Protein

Envelope glycoprotein

Gene

GP

Organism
Bunyamwera virus (BUNV)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Glycoprotein G1 and glycoprotein G2 interact with each other and are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei477 – 4782Cleavage; by host signal peptidaseBy similarity

GO - Biological processi

  1. fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
  2. modulation by virus of host morphology or physiology Source: InterPro
  3. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Envelope glycoprotein
Short name:
GP
Alternative name(s):
M polyprotein
Cleaved into the following 3 chains:
Gene namesi
Name:GP
OrganismiBunyamwera virus (BUNV)
Taxonomic identifieri35304 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesBunyaviridaeOrthobunyavirus
Virus hostiAedes aegypti (Yellowfever mosquito) (Culex aegypti) [TaxID: 7159]
Homo sapiens (Human) [TaxID: 9606]
ProteomesiUP000002476: Genome

Subcellular locationi

Chain Glycoprotein G1 : Virion membrane Curated; Single-pass type I membrane protein Curated. Host Golgi apparatus membrane Curated; Single-pass type I membrane protein Curated. Host endoplasmic reticulum membrane Curated; Single-pass type I membrane protein Curated
Note: G1 alone is retained in the membrane of the endoplasmic reticulum, but not transported to the Golgi. Coexpression of G1 and G2 results in efficient transport of G1 to the Golgi complex, indicating that their interaction is essential for proper targeting to this organelle, where virion budding occurs.
Chain Glycoprotein G2 : Virion membrane Curated; Single-pass type I membrane protein Curated. Host Golgi apparatus membrane Curated; Single-pass type I membrane protein Curated
Note: G2 is retained in the Golgi complex through a Golgi retention signal, which resides in the G2 transmembrane region.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini17 – 203187LumenalSequence AnalysisAdd
BLAST
Transmembranei204 – 22421HelicalSequence AnalysisAdd
BLAST
Topological domaini225 – 30884CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei309 – 32921HelicalSequence AnalysisAdd
BLAST
Topological domaini330 – 36940LumenalSequence AnalysisAdd
BLAST
Transmembranei370 – 39021HelicalSequence AnalysisAdd
BLAST
Topological domaini391 – 45666CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei457 – 47721HelicalSequence AnalysisAdd
BLAST
Topological domaini478 – 1387910LumenalSequence AnalysisAdd
BLAST
Transmembranei1388 – 140821HelicalSequence AnalysisAdd
BLAST
Topological domaini1409 – 143325CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. host cell endoplasmic reticulum Source: UniProtKB-KW
  2. host cell Golgi apparatus Source: UniProtKB-KW
  3. host cell membrane Source: UniProtKB-KW
  4. integral component of membrane Source: UniProtKB-KW
  5. virion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host endoplasmic reticulum, Host Golgi apparatus, Host membrane, Membrane, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616Add
BLAST
Chaini17 – 14331417Envelope glycoproteinPRO_0000036798Add
BLAST
Chaini17 – 302286Glycoprotein G2By similarityPRO_0000036799Add
BLAST
Chaini303 – 477175Non-structural protein NSmBy similarityPRO_0000036800Add
BLAST
Chaini478 – 1433956Glycoprotein G1By similarityPRO_0000036801Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi60 – 601N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi624 – 6241N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi1169 – 11691N-linked (GlcNAc...); by hostSequence Analysis

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins including nonstructural protein NSm, glycoprotein G1, and glycoprotein G2.

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

G1 and G2 interacts with each other.By similarity

Protein-protein interaction databases

DIPiDIP-404N.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR005167. Bunya_G1.
IPR005168. Bunya_G2.
IPR026400. Bunya_nonstruc_pro_NSm.
IPR014413. M_poly_OrthobunV.
[Graphical view]
PfamiPF03557. Bunya_G1. 1 hit.
PF03563. Bunya_G2. 1 hit.
[Graphical view]
PIRSFiPIRSF003944. M_poly_OrthobunV. 1 hit.
TIGRFAMsiTIGR04210. bunya_NSm. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04505-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MRILILLLAV TQLAVSSPVI TRCFHGGQLI AERKSQTSIS EFCIKDDVSM
60 70 80 90 100
LKSEIVYTKN DTGIFGHSKV FRHWTITDWK ACNPVVTAGG SINVIEVDKN
110 120 130 140 150
LNLVTRNYVC TGDCTITVDR KNAQIIFQTD KLNHFEVTGT TISTGWFKSK
160 170 180 190 200
ASVTLDRTCE HIKVSCGKKT LQFHACFKQH MSCVRFLHRS ILPGSMAISI
210 220 230 240 250
CQNIELIIIT ILALCIFIIM IILTKTYICY VLIPVFMPIA FAYGWAYNRS
260 270 280 290 300
CKKCTCCGLA YHPFTNCGSY CVCGSKFETS DRMRMHRESG LCQGFKSLRV
310 320 330 340 350
ARRLCKSKGS SLIISILLSV LILSFVTPIE GTLTNYPTDQ KYTLDEIADV
360 370 380 390 400
LQAKTHEDST KYYIILYTSL FGAGLTIIFA GVALGLTIIL EVLTKINVIF
410 420 430 440 450
CNECNMYHSK KSIKYVGDFT NKCGFCTCGL LEDPEGVVVH KAKKSCTYSY
460 470 480 490 500
QINWVRGIMI FVAFLFVIQN TIIMVAAEED CWKNEELKED CVGPLIAPKD
510 520 530 540 550
CTDKDHKTYL SEASLLATAK KITQVDAENV EILGKTMESA IRVIERQKTY
560 570 580 590 600
HRMHLLEAVF LNKHCDYYKM FEHNSGYSQV KWRMMIKTQH FDICALQANS
610 620 630 640 650
PFCAQCIADN SCAQGSWEFD THMNSTYSSK VDNFKHDFSL FLRIFEAAFP
660 670 680 690 700
GTAYVHLLTN IKEKKPYQAV SMIEKIKKKF PNNKLLIGYL DFGKYLLGLS
710 720 730 740 750
HASTYELQQR QLDKLYQPTE LTRSGGQQTS LANSVVGQAT KECKKYKDVS
760 770 780 790 800
CLSPRFGIPL EDLISCCDQP NYNIYKKPKK VYKAHDKEET WCINDQHCLV
810 820 830 840 850
DFVPAEADTV EKLKPMKCWL VDPGKNDDVY SIAIKTCRVV DKGVCTVNSQ
860 870 880 890 900
KWNIIKCDSG PLYYSDHIPG EDTGNDIGHY CVSAGCKTDR YPINPDVVTD
910 920 930 940 950
CVWEFTSRKS QYIGKISMQS LEDYEKALTD RLTHTLETYS FAPLENLPHI
960 970 980 990 1000
KPVYKYITAQ GVENSDGIEG AFITASIPAA GGTSIGYNVR SKDGFPLLDL
1010 1020 1030 1040 1050
IVFVKSAVIK STYNHIYDTG PTISINTKHD EHCTGQCPSN IEHEANWLTF
1060 1070 1080 1090 1100
SQERTSRWGC EEFGCLAVNT GCVFGSCQDV IRPETKVYRK AVDEVVILTV
1110 1120 1130 1140 1150
CITYPGHTFC TEINAIEPKI TEEIELQFKT VDTKTLPYIV AVNNHKLYSG
1160 1170 1180 1190 1200
QINDLGTFGQ MCGNVQKTNS SILGTGTPKF DYTCHGASRK DIIVRRCYNN
1210 1220 1230 1240 1250
NFDSCKLLKE ETQLIFNDDH DTITVYNTNH LIGELAIKLI LGDIQYKLFT
1260 1270 1280 1290 1300
ETLDLQIDAK CVGCPDCFES YSCNFQIVSN IDTICSLEGP CDTFHNRISI
1310 1320 1330 1340 1350
KAMQQNYAVK LSCQKDPRPS GTFKICNREY TVVFHTVAKD DKIEINVGDQ
1360 1370 1380 1390 1400
TSFIKEKDDR CKTWLCRVRD EGISVIFEPI KAFFGSYFSI FFYIIVVVVV
1410 1420 1430
GFLIIYIFMP MFMKLKEVLK ANEKLYLQEI KQK
Length:1,433
Mass (Da):162,078
Last modified:August 13, 1987 - v1
Checksum:iCD61ABDE782018E0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11852 Genomic RNA. Translation: AAA42777.1.
PIRiA04101. GNVUBW.
RefSeqiNP_047212.1. NC_001926.1.

Genome annotation databases

GeneIDi2648216.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11852 Genomic RNA. Translation: AAA42777.1 .
PIRi A04101. GNVUBW.
RefSeqi NP_047212.1. NC_001926.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-404N.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2648216.

Family and domain databases

InterProi IPR005167. Bunya_G1.
IPR005168. Bunya_G2.
IPR026400. Bunya_nonstruc_pro_NSm.
IPR014413. M_poly_OrthobunV.
[Graphical view ]
Pfami PF03557. Bunya_G1. 1 hit.
PF03563. Bunya_G2. 1 hit.
[Graphical view ]
PIRSFi PIRSF003944. M_poly_OrthobunV. 1 hit.
TIGRFAMsi TIGR04210. bunya_NSm. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the Bunyamwera virus M RNA segment: conservation of structural features in the Bunyavirus glycoprotein gene product."
    Lees J.F., Pringle C.R., Elliott R.M.
    Virology 148:1-14(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Localization of Bunyamwera bunyavirus G1 glycoprotein to the Golgi requires association with G2 but not with NSm."
    Lappin D.F., Nakitare G.W., Palfreyman J.W., Elliott R.M.
    J. Gen. Virol. 75:3441-3451(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  3. "Mapping the Golgi targeting and retention signal of Bunyamwera virus glycoproteins."
    Shi X., Lappin D.F., Elliott R.M.
    J. Virol. 78:10793-10802(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  4. "Requirement of the N-terminal region of orthobunyavirus nonstructural protein NSm for virus assembly and morphogenesis."
    Shi X., Kohl A., Leonard V.H., Li P., McLees A., Elliott R.M.
    J. Virol. 80:8089-8099(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.

Entry informationi

Entry nameiGP_BUNYW
AccessioniPrimary (citable) accession number: P04505
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: October 29, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3