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Protein

Envelope glycoprotein E

Gene

gE

Organism
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

In epithelial cells, the heterodimer gE/gI is required for the cell-to-cell spread of the virus, by sorting nascent virions to cell junctions. Once the virus reaches the cell junctions, virus particles can spread to adjacent cells extremely rapidly through interactions with cellular receptors that accumulate at these junctions. Implicated in basolateral spread in polarized cells (By similarity). In neuronal cells, gE/gI is essential for the anterograde spread of the infection throughout the host nervous system. Together with US9, the heterodimer gE/gI is involved in the sorting and transport of viral structural components toward axon tips.By similarity2 Publications
The heterodimer gE/gI serves as a receptor for the Fc part of host IgG. Dissociation of gE/gI from IgG occurs at acidic pH. May thus be involved in anti-HSV antibodies bipolar bridging, followed by intracellular endocytosis and degradation, thereby interfering with host IgG-mediated immune responses.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Viral immunoevasion

Names & Taxonomyi

Protein namesi
Recommended name:
Envelope glycoprotein E
Short name:
gE
Alternative name(s):
gE-1
Gene namesi
Name:gE
ORF Names:US8
OrganismiHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Taxonomic identifieri10299 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000009294 Componenti: Genome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 419Virion surfaceSequence analysisAdd BLAST399
Transmembranei420 – 440HelicalSequence analysisAdd BLAST21
Topological domaini441 – 550IntravirionSequence analysisAdd BLAST110

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cell junction, Host cell membrane, Host endosome, Host Golgi apparatus, Host membrane, Membrane, Viral envelope protein, Virion

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2364696.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000003822521 – 550Envelope glycoprotein EAdd BLAST530

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi124N-linked (GlcNAc...); by hostSequence analysis1
Modified residuei176SulfotyrosineSequence analysis1
Glycosylationi243N-linked (GlcNAc...); by hostSequence analysis1
Disulfide bondi271 ↔ 2971 Publication
Disulfide bondi280 ↔ 2891 Publication
Disulfide bondi314 ↔ 3231 Publication
Modified residuei476Phosphoserine; by host CK21 Publication1
Modified residuei477Phosphoserine; by host CK21 Publication1
Modified residuei503PhosphoserineCurated1

Post-translational modificationi

Phosphorylated on serines within the acidic cluster. Phosphorylation determines whether endocytosed viral gE traffics to the trans-Golgi network or recycles to the cell membrane.Curated
N-glycosylated, and sulfated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Sulfation

Proteomic databases

PRIDEiP04488.

PTM databases

iPTMnetiP04488.

Interactioni

Subunit structurei

Interacts with gI; this interaction enhances the Fc receptor function of gE (By similarity). The heterodimer gE/gI interacts with the Fc part of host IgG. Interacts (via C-terminus) with VP22 tegument protein; this interaction is necessary for the recruitment of VP22 to the Golgi and its packaging into virions. Interacts (via C-terminus) with UL11 tegument protein.By similarity5 Publications

Protein-protein interaction databases

DIPiDIP-29186N.

Structurei

Secondary structure

1550
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi221 – 227Combined sources7
Beta strandi230 – 233Combined sources4
Beta strandi245 – 253Combined sources9
Beta strandi256 – 265Combined sources10
Beta strandi272 – 277Combined sources6
Helixi278 – 282Combined sources5
Helixi287 – 290Combined sources4
Helixi295 – 297Combined sources3
Beta strandi306 – 315Combined sources10
Turni320 – 323Combined sources4
Beta strandi324 – 329Combined sources6
Beta strandi345 – 348Combined sources4
Helixi351 – 353Combined sources3
Beta strandi355 – 363Combined sources9
Beta strandi366 – 376Combined sources11
Turni378 – 380Combined sources3
Beta strandi383 – 387Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GIYX-ray1.78A/B213-390[»]
2GJ7X-ray5.00E/F21-419[»]
SMRiP04488.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04488.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni63 – 88Interaction with gIBy similarityAdd BLAST26
Regioni235 – 380Fc-bindingAdd BLAST146
Regioni470 – 495Interaction with VP22 and UL111 PublicationAdd BLAST26
Regioni476 – 484Acidic9

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi463 – 466Internalization motifSequence analysis4
Motifi472 – 475Internalization motifSequence analysis4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi163 – 214Pro-richAdd BLAST52

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR003404. Herpes_glycopE.
IPR007110. Ig-like_dom.
[Graphical view]
PfamiPF02480. Herpes_gE. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04488-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRGAVVGFL LGVCVVSCLA GTPKTSWRRV SVGEDVSLLP APGPTGRGPT
60 70 80 90 100
QKLLWAVEPL DGCGPLHPSW VSLMPPKQVP ETVVDAACMR APVPLAMAYA
110 120 130 140 150
PPAPSATGGL RTDFVWQERA AVVNRSLVIH GVRETDSGLY TLSVGDIKDP
160 170 180 190 200
ARQVASVVLV VQPAPVPTPP PTPADYDEDD NDEGEDESLA GTPASGTPRL
210 220 230 240 250
PPPPAPPRSW PSAPEVSHVR GVTVRMETPE AILFSPGETF STNVSIHAIA
260 270 280 290 300
HDDQTYSMDV VWLRFDVPTS CAEMRIYESC LYHPQLPECL SPADAPCAAS
310 320 330 340 350
TWTSRLAVRS YAGCSRTNPP PRCSAEAHME PVPGLAWQAA SVNLEFRDAS
360 370 380 390 400
PQHSGLYLCV VYVNDHIHAW GHITISTAAQ YRNAVVEQPL PQRGADLAEP
410 420 430 440 450
THPHVGAPPH APPTHGALRL GAVMGAALLL SALGLSVWAC MTCWRRRAWR
460 470 480 490 500
AVKSRASGKG PTYIRVADSE LYADWSSDSE GERDQVPWLA PPERPDSPST
510 520 530 540 550
NGSGFEILSP TAPSVYPRSD GHQSRRQLTT FGSGRPDRRY SQASDSSVFW
Length:550
Mass (Da):59,094
Last modified:August 13, 1987 - v1
Checksum:iBE8271E5B7E34776
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti120A → T in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti130H → Y in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti143S → F in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti239T → A in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti257S → A in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti432A → V in strain: Nonneuroinvasive mutant HF10. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14112 Genomic DNA. Translation: CAA32272.1.
X02138 Genomic DNA. Translation: CAA26062.1.
L00036 Genomic DNA. Translation: AAA96680.1.
DQ889502 Genomic DNA. Translation: ABI63526.1.
FJ593289 Genomic DNA. Translation: ACM62297.1.
PIRiA03733. VGBE18.
RefSeqiYP_009137143.1. NC_001806.2.

Genome annotation databases

GeneIDi2703448.
KEGGivg:2703448.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14112 Genomic DNA. Translation: CAA32272.1.
X02138 Genomic DNA. Translation: CAA26062.1.
L00036 Genomic DNA. Translation: AAA96680.1.
DQ889502 Genomic DNA. Translation: ABI63526.1.
FJ593289 Genomic DNA. Translation: ACM62297.1.
PIRiA03733. VGBE18.
RefSeqiYP_009137143.1. NC_001806.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GIYX-ray1.78A/B213-390[»]
2GJ7X-ray5.00E/F21-419[»]
SMRiP04488.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29186N.

Chemistry databases

ChEMBLiCHEMBL2364696.

PTM databases

iPTMnetiP04488.

Proteomic databases

PRIDEiP04488.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2703448.
KEGGivg:2703448.

Miscellaneous databases

EvolutionaryTraceiP04488.
PROiP04488.

Family and domain databases

InterProiIPR003404. Herpes_glycopE.
IPR007110. Ig-like_dom.
[Graphical view]
PfamiPF02480. Herpes_gE. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiGE_HHV11
AccessioniPrimary (citable) accession number: P04488
Secondary accession number(s): B9VQK2, Q09I69
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: November 2, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.