ID VP16_HHV1F Reviewed; 490 AA. AC P04486; DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot. DT 22-SEP-2009, sequence version 2. DT 31-MAY-2011, entry version 59. DE RecName: Full=Tegument protein VP16; DE AltName: Full=Alpha trans-inducing protein; DE AltName: Full=Alpha-TIF; DE AltName: Full=ICP25; DE AltName: Full=Vmw65; GN ORFNames=UL48; OS Human herpesvirus 1 (strain F) (HHV-1) (Human herpes simplex virus 1). OC Viruses; dsDNA viruses, no RNA stage; Herpesvirales; Herpesviridae; OC Alphaherpesvirinae; Simplexvirus. OX NCBI_TaxID=10304; OH NCBI_TaxID=9606; Homo sapiens (Human). RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX MEDLINE=85298259; PubMed=2994050; DOI=10.1073/pnas.82.17.5870; RA Pellett P.E., McKnight J.L.C., Jenkins F.J., Roizman B.; RT "Nucleotide sequence and predicted amino acid sequence of a protein RT encoded in a small herpes simplex virus DNA fragment capable of trans- RT inducing alpha genes."; RL Proc. Natl. Acad. Sci. U.S.A. 82:5870-5874(1985). RN [2] RP FUNCTION, AND INTERACTION WITH HUMAN HCFC1. RX PubMed=15522876; DOI=10.1074/jbc.M410178200; RA Narayanan A., Nogueira M.L., Ruyechan W.T., Kristie T.M.; RT "Combinatorial transcription of herpes simplex virus and varicella RT zoster virus immediate early genes is strictly determined by the RT cellular coactivator HCF-1."; RL J. Biol. Chem. 280:1369-1375(2005). RN [3] RP SUBCELLULAR LOCATION. RX PubMed=15795259; DOI=10.1128/JVI.79.8.4730-4743.2005; RA Yedowitz J.C., Kotsakis A., Schlegel E.F., Blaho J.A.; RT "Nuclear localizations of the herpes simplex virus type 1 tegument RT proteins VP13/14, vhs, and VP16 precede VP22-dependent microtubule RT reorganization and VP22 nuclear import."; RL J. Virol. 79:4730-4743(2005). RN [4] RP SUBCELLULAR LOCATION. RX PubMed=18596102; DOI=10.1128/JVI.00904-08; RA Loret S., Guay G., Lippe R.; RT "Comprehensive characterization of extracellular herpes simplex virus RT type 1 virions."; RL J. Virol. 82:8605-8618(2008). RN [5] RP STRUCTURE BY NMR OF 445-479 IN COMPLEX WITH THE TFB1 SUBUNIT OF TFIIH. RX PubMed=18630911; DOI=10.1021/ja800975h; RA Langlois C., Mas C., Di Lello P., Jenkins L.M., Legault P., RA Omichinski J.G.; RT "NMR structure of the complex between the Tfb1 subunit of TFIIH and RT the activation domain of VP16: structural similarities between VP16 RT and p53."; RL J. Am. Chem. Soc. 130:10596-10604(2008). CC -!- FUNCTION: Transcriptional activator of immediate-early (IE) gene CC products (alpha genes). Acts as a key activator of lytic infection CC by initiating the lytic program through the assembly of the CC transcriptional regulatory VP16-induced complex composed of VP16 CC and two cellular factors, HCFC1 and POU2F 1. VP16-induced complex CC represents a regulatory switch: when it is on, it promotes IE-gene CC expression and thus lytic infection, and when it is off, it limits CC IE-gene transcription favoring latent infection (By similarity). CC -!- FUNCTION: May play a role in the aggregation of tegument proteins CC around nucleocapsids during virus morphogenesis (Potential). CC -!- SUBUNIT: Interacts with VP22. Interacts with gH (via C-terminus). CC Interacts with the virion host shutoff protein (vhs). Interacts CC with VP11/12. Associates with the VP16-induced complex; binding to CC host HCFC1 activates VP16 for association with the octamer motif- CC binding host protein POU2F1, to form a multiprotein-DNA complex CC responsible for activating transcription of the viral immediate CC early genes (By similarity). CC -!- SUBCELLULAR LOCATION: Virion tegument. Host nucleus. CC -!- DOMAIN: The transcriptional activation region seems to target many CC proteins of the RNA polymerase II transcription machinery (By CC similarity). CC -!- SIMILARITY: Belongs to the herpesviridae tegument protein VP16 CC protein family. CC -!- SEQUENCE CAUTION: CC Sequence=AAA45766.1; Type=Erroneous initiation; CC ----------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution-NoDerivs License CC ----------------------------------------------------------------------- DR EMBL; K03350; AAA45766.1; ALT_INIT; Genomic_DNA. DR PIR; A03727; IXBE1F. DR PDB; 2K2U; NMR; -; B=456-490. DR PDBsum; 2K2U; -. DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell. DR GO; GO:0019033; C:viral tegument; IEA:UniProtKB-SubCell. DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW. DR GO; GO:0044419; P:interspecies interaction between organisms; IEA:UniProtKB-KW. DR GO; GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW. DR GO; GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. DR InterPro; IPR003174; Alpha_TIF. DR InterPro; IPR021051; HSV_VP16_C. DR Gene3D; G3DSA:1.10.1290.10; Alpha_TIF; 1. DR Pfam; PF02232; Alpha_TIF; 1. DR Pfam; PF12149; HSV_VP16_C; 1. DR SMART; SM00814; Alpha_TIF; 1. DR SUPFAM; SSF56548; VP16_like; 1. PE 1: Evidence at protein level; KW 3D-structure; DNA-binding; Host nucleus; Host-virus interaction; KW Phosphoprotein; Transcription; Transcription regulation; Virion; KW Virion tegument. FT CHAIN 1 490 Tegument protein VP16. FT /FTId=PRO_0000115799. FT REGION 411 490 Transcriptional activation (By FT similarity). FT SITE 442 442 Critical role in activation (By FT similarity). FT MOD_RES 18 18 Phosphoserine (By similarity). FT MOD_RES 353 353 Phosphoserine (By similarity). FT MOD_RES 411 411 Phosphoserine (By similarity). FT MOD_RES 452 452 Phosphoserine (By similarity). FT HELIX 460 466 SQ SEQUENCE 490 AA; 54316 MW; 014372DE8FE6C417 CRC64; MDLLVDELFA DMDADGASPP PPRPAGGPKN TPAAPPLYAT GRLSQAQLMP SPPMPVPPAA LFNRLLDDLG FSAGPALCTM LDTWNEDLFS ALPTNADLYR ECKFLSTLPS DVVEWGDAYV PERAQIDIRA HGDVAFPTLP ATRDGLGLYY EALSRFFHAE LRAREESYRT VLANFCSALY RYLRASVRQL HRQAHMRGRD RDLGEMLRAT IADRYYRETA RLARVLFLHL YLFLTREILW AAYAEQMMRP DLFDCLCCDL ESWRQLAGLF QPFMFVNGAL TVRGVPIEAR RLRELNHIRE HLNLPLVRSA ATEEPGAPLT TPPTLHGNQA RASGYFMVLI RAKLDSYSSF TTSPSEAVMR EHAYSRARTK NNYGSTIEGL LDLPDDDAPE EAGLAAPRLS FLPAGHTRRL STAPPTDVSL GDELHLDGED VAMAHADALD DFDLDMLGDG DSPGPGFTPH DSAPYGALDM ADFEFEQMFT DALGIDEYGG //