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P04486

- VP16_HHV1F

UniProt

P04486 - VP16_HHV1F

Protein

Tegument protein VP16

Gene

UL48

Organism
Human herpesvirus 1 (strain F) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 2 (22 Sep 2009)
      Previous versions | rss
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    Functioni

    Transcriptional activator of immediate-early (IE) gene products (alpha genes). Acts as a key activator of lytic infection by initiating the lytic program through the assembly of the transcriptional regulatory VP16-induced complex composed of VP16 and two cellular factors, HCFC1 and POU2F 1. VP16-induced complex represents a regulatory switch: when it is on, it promotes IE-gene expression and thus lytic infection, and when it is off, it limits IE-gene transcription favoring latent infection By similarity.By similarity
    May play a role in the aggregation of tegument proteins around nucleocapsids during virus morphogenesis.Curated

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei442 – 4421Critical role in activationBy similarity

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. regulation of transcription, DNA-templated Source: UniProtKB-KW
    2. transcription, DNA-templated Source: UniProtKB-KW
    3. viral process Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tegument protein VP16
    Alternative name(s):
    Alpha trans-inducing protein
    Alpha-TIF
    ICP25
    Vmw65
    Gene namesi
    ORF Names:UL48
    OrganismiHuman herpesvirus 1 (strain F) (HHV-1) (Human herpes simplex virus 1)
    Taxonomic identifieri10304 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]

    Subcellular locationi

    GO - Cellular componenti

    1. host cell nucleus Source: UniProtKB-SubCell
    2. viral tegument Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host nucleus, Virion, Virion tegument

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 490490Tegument protein VP16PRO_0000115799Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei18 – 181PhosphoserineBy similarity
    Modified residuei353 – 3531PhosphoserineBy similarity
    Modified residuei411 – 4111PhosphoserineBy similarity
    Modified residuei452 – 4521PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Subunit structurei

    Interacts with VP22. Interacts with gH (via C-terminus). Interacts with the virion host shutoff protein (vhs). Interacts with VP11/12. Associates with the VP16-induced complex; binding to host HCFC1 activates VP16 for association with the octamer motif-binding host protein POU2F1, to form a multiprotein-DNA complex responsible for activating transcription of the viral immediate early genes By similarity.By similarity

    Structurei

    Secondary structure

    1
    490
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi471 – 4777

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2K2UNMR-B456-490[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP04486.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni411 – 49080Transcriptional activationBy similarityAdd
    BLAST

    Domaini

    The transcriptional activation region seems to target many proteins of the RNA polymerase II transcription machinery.By similarity

    Sequence similaritiesi

    Family and domain databases

    Gene3Di1.10.1290.10. 1 hit.
    InterProiIPR003174. Alpha_TIF.
    IPR021051. HSV_VP16_C.
    [Graphical view]
    PfamiPF02232. Alpha_TIF. 1 hit.
    PF12149. HSV_VP16_C. 1 hit.
    [Graphical view]
    SMARTiSM00814. Alpha_TIF. 1 hit.
    [Graphical view]
    SUPFAMiSSF56548. SSF56548. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P04486-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDLLVDELFA DMDADGASPP PPRPAGGPKN TPAAPPLYAT GRLSQAQLMP    50
    SPPMPVPPAA LFNRLLDDLG FSAGPALCTM LDTWNEDLFS ALPTNADLYR 100
    ECKFLSTLPS DVVEWGDAYV PERAQIDIRA HGDVAFPTLP ATRDGLGLYY 150
    EALSRFFHAE LRAREESYRT VLANFCSALY RYLRASVRQL HRQAHMRGRD 200
    RDLGEMLRAT IADRYYRETA RLARVLFLHL YLFLTREILW AAYAEQMMRP 250
    DLFDCLCCDL ESWRQLAGLF QPFMFVNGAL TVRGVPIEAR RLRELNHIRE 300
    HLNLPLVRSA ATEEPGAPLT TPPTLHGNQA RASGYFMVLI RAKLDSYSSF 350
    TTSPSEAVMR EHAYSRARTK NNYGSTIEGL LDLPDDDAPE EAGLAAPRLS 400
    FLPAGHTRRL STAPPTDVSL GDELHLDGED VAMAHADALD DFDLDMLGDG 450
    DSPGPGFTPH DSAPYGALDM ADFEFEQMFT DALGIDEYGG 490
    Length:490
    Mass (Da):54,316
    Last modified:September 22, 2009 - v2
    Checksum:i014372DE8FE6C417
    GO

    Sequence cautioni

    The sequence AAA45766.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K03350 Genomic DNA. Translation: AAA45766.1. Different initiation.
    PIRiA03727. IXBE1F.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K03350 Genomic DNA. Translation: AAA45766.1 . Different initiation.
    PIRi A03727. IXBE1F.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2K2U NMR - B 456-490 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P04486.

    Family and domain databases

    Gene3Di 1.10.1290.10. 1 hit.
    InterProi IPR003174. Alpha_TIF.
    IPR021051. HSV_VP16_C.
    [Graphical view ]
    Pfami PF02232. Alpha_TIF. 1 hit.
    PF12149. HSV_VP16_C. 1 hit.
    [Graphical view ]
    SMARTi SM00814. Alpha_TIF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56548. SSF56548. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence and predicted amino acid sequence of a protein encoded in a small herpes simplex virus DNA fragment capable of trans-inducing alpha genes."
      Pellett P.E., McKnight J.L.C., Jenkins F.J., Roizman B.
      Proc. Natl. Acad. Sci. U.S.A. 82:5870-5874(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Combinatorial transcription of herpes simplex virus and varicella zoster virus immediate early genes is strictly determined by the cellular coactivator HCF-1."
      Narayanan A., Nogueira M.L., Ruyechan W.T., Kristie T.M.
      J. Biol. Chem. 280:1369-1375(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH HUMAN HCFC1.
    3. "Nuclear localizations of the herpes simplex virus type 1 tegument proteins VP13/14, vhs, and VP16 precede VP22-dependent microtubule reorganization and VP22 nuclear import."
      Yedowitz J.C., Kotsakis A., Schlegel E.F., Blaho J.A.
      J. Virol. 79:4730-4743(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    4. "Comprehensive characterization of extracellular herpes simplex virus type 1 virions."
      Loret S., Guay G., Lippe R.
      J. Virol. 82:8605-8618(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    5. "NMR structure of the complex between the Tfb1 subunit of TFIIH and the activation domain of VP16: structural similarities between VP16 and p53."
      Langlois C., Mas C., Di Lello P., Jenkins L.M., Legault P., Omichinski J.G.
      J. Am. Chem. Soc. 130:10596-10604(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 445-479 IN COMPLEX WITH THE TFB1 SUBUNIT OF TFIIH.

    Entry informationi

    Entry nameiVP16_HHV1F
    AccessioniPrimary (citable) accession number: P04486
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: September 22, 2009
    Last modified: October 1, 2014
    This is version 68 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3