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P04486

- VP16_HHV1F

UniProt

P04486 - VP16_HHV1F

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Protein

Tegument protein VP16

Gene

UL48

Organism
Human herpesvirus 1 (strain F) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Transcriptional activator of immediate-early (IE) gene products (alpha genes). Acts as a key activator of lytic infection by initiating the lytic program through the assembly of the transcriptional regulatory VP16-induced complex composed of VP16 and two cellular factors, HCFC1 and POU2F 1. VP16-induced complex represents a regulatory switch: when it is on, it promotes IE-gene expression and thus lytic infection, and when it is off, it limits IE-gene transcription favoring latent infection (By similarity).By similarity
May play a role in the aggregation of tegument proteins around nucleocapsids during virus morphogenesis.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei442 – 4421Critical role in activationBy similarity

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
  3. viral process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tegument protein VP16
Alternative name(s):
Alpha trans-inducing protein
Alpha-TIF
ICP25
Vmw65
Gene namesi
ORF Names:UL48
OrganismiHuman herpesvirus 1 (strain F) (HHV-1) (Human herpes simplex virus 1)
Taxonomic identifieri10304 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]

Subcellular locationi

GO - Cellular componenti

  1. host cell nucleus Source: UniProtKB-KW
  2. viral tegument Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 490490Tegument protein VP16PRO_0000115799Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei18 – 181PhosphoserineBy similarity
Modified residuei353 – 3531PhosphoserineBy similarity
Modified residuei411 – 4111PhosphoserineBy similarity
Modified residuei452 – 4521PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Interacts with VP22. Interacts with gH (via C-terminus). Interacts with the virion host shutoff protein (vhs). Interacts with VP11/12. Associates with the VP16-induced complex; binding to host HCFC1 activates VP16 for association with the octamer motif-binding host protein POU2F1, to form a multiprotein-DNA complex responsible for activating transcription of the viral immediate early genes (By similarity).By similarity

Structurei

Secondary structure

1
490
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi471 – 4777Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2K2UNMR-B456-490[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04486.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni411 – 49080Transcriptional activationBy similarityAdd
BLAST

Domaini

The transcriptional activation region seems to target many proteins of the RNA polymerase II transcription machinery.By similarity

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.1290.10. 1 hit.
InterProiIPR003174. Alpha_TIF.
IPR021051. HSV_VP16_C.
[Graphical view]
PfamiPF02232. Alpha_TIF. 1 hit.
PF12149. HSV_VP16_C. 1 hit.
[Graphical view]
SMARTiSM00814. Alpha_TIF. 1 hit.
[Graphical view]
SUPFAMiSSF56548. SSF56548. 1 hit.

Sequencei

Sequence statusi: Complete.

P04486-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDLLVDELFA DMDADGASPP PPRPAGGPKN TPAAPPLYAT GRLSQAQLMP
60 70 80 90 100
SPPMPVPPAA LFNRLLDDLG FSAGPALCTM LDTWNEDLFS ALPTNADLYR
110 120 130 140 150
ECKFLSTLPS DVVEWGDAYV PERAQIDIRA HGDVAFPTLP ATRDGLGLYY
160 170 180 190 200
EALSRFFHAE LRAREESYRT VLANFCSALY RYLRASVRQL HRQAHMRGRD
210 220 230 240 250
RDLGEMLRAT IADRYYRETA RLARVLFLHL YLFLTREILW AAYAEQMMRP
260 270 280 290 300
DLFDCLCCDL ESWRQLAGLF QPFMFVNGAL TVRGVPIEAR RLRELNHIRE
310 320 330 340 350
HLNLPLVRSA ATEEPGAPLT TPPTLHGNQA RASGYFMVLI RAKLDSYSSF
360 370 380 390 400
TTSPSEAVMR EHAYSRARTK NNYGSTIEGL LDLPDDDAPE EAGLAAPRLS
410 420 430 440 450
FLPAGHTRRL STAPPTDVSL GDELHLDGED VAMAHADALD DFDLDMLGDG
460 470 480 490
DSPGPGFTPH DSAPYGALDM ADFEFEQMFT DALGIDEYGG
Length:490
Mass (Da):54,316
Last modified:September 22, 2009 - v2
Checksum:i014372DE8FE6C417
GO

Sequence cautioni

The sequence AAA45766.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K03350 Genomic DNA. Translation: AAA45766.1. Different initiation.
PIRiA03727. IXBE1F.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K03350 Genomic DNA. Translation: AAA45766.1 . Different initiation.
PIRi A03727. IXBE1F.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2K2U NMR - B 456-490 [» ]
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P04486.

Family and domain databases

Gene3Di 1.10.1290.10. 1 hit.
InterProi IPR003174. Alpha_TIF.
IPR021051. HSV_VP16_C.
[Graphical view ]
Pfami PF02232. Alpha_TIF. 1 hit.
PF12149. HSV_VP16_C. 1 hit.
[Graphical view ]
SMARTi SM00814. Alpha_TIF. 1 hit.
[Graphical view ]
SUPFAMi SSF56548. SSF56548. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence and predicted amino acid sequence of a protein encoded in a small herpes simplex virus DNA fragment capable of trans-inducing alpha genes."
    Pellett P.E., McKnight J.L.C., Jenkins F.J., Roizman B.
    Proc. Natl. Acad. Sci. U.S.A. 82:5870-5874(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Combinatorial transcription of herpes simplex virus and varicella zoster virus immediate early genes is strictly determined by the cellular coactivator HCF-1."
    Narayanan A., Nogueira M.L., Ruyechan W.T., Kristie T.M.
    J. Biol. Chem. 280:1369-1375(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HUMAN HCFC1.
  3. "Nuclear localizations of the herpes simplex virus type 1 tegument proteins VP13/14, vhs, and VP16 precede VP22-dependent microtubule reorganization and VP22 nuclear import."
    Yedowitz J.C., Kotsakis A., Schlegel E.F., Blaho J.A.
    J. Virol. 79:4730-4743(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  4. "Comprehensive characterization of extracellular herpes simplex virus type 1 virions."
    Loret S., Guay G., Lippe R.
    J. Virol. 82:8605-8618(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  5. "NMR structure of the complex between the Tfb1 subunit of TFIIH and the activation domain of VP16: structural similarities between VP16 and p53."
    Langlois C., Mas C., Di Lello P., Jenkins L.M., Legault P., Omichinski J.G.
    J. Am. Chem. Soc. 130:10596-10604(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 445-479 IN COMPLEX WITH THE TFB1 SUBUNIT OF TFIIH.

Entry informationi

Entry nameiVP16_HHV1F
AccessioniPrimary (citable) accession number: P04486
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: September 22, 2009
Last modified: November 26, 2014
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3