Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P04486 (VP16_HHV1F) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tegument protein VP16
Alternative name(s):
Alpha trans-inducing protein
Alpha-TIF
ICP25
Vmw65
Gene names
ORF Names:UL48
OrganismHuman herpesvirus 1 (strain F) (HHV-1) (Human herpes simplex virus 1)
Taxonomic identifier10304 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length490 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional activator of immediate-early (IE) gene products (alpha genes). Acts as a key activator of lytic infection by initiating the lytic program through the assembly of the transcriptional regulatory VP16-induced complex composed of VP16 and two cellular factors, HCFC1 and POU2F 1. VP16-induced complex represents a regulatory switch: when it is on, it promotes IE-gene expression and thus lytic infection, and when it is off, it limits IE-gene transcription favoring latent infection By similarity. Ref.2

May play a role in the aggregation of tegument proteins around nucleocapsids during virus morphogenesis Potential. Ref.2

Subunit structure

Interacts with VP22. Interacts with gH (via C-terminus). Interacts with the virion host shutoff protein (vhs). Interacts with VP11/12. Associates with the VP16-induced complex; binding to host HCFC1 activates VP16 for association with the octamer motif-binding host protein POU2F1, to form a multiprotein-DNA complex responsible for activating transcription of the viral immediate early genes By similarity. Ref.2 Ref.5

Subcellular location

Virion tegument. Host nucleus Ref.3 Ref.4.

Domain

The transcriptional activation region seems to target many proteins of the RNA polymerase II transcription machinery By similarity.

Sequence similarities

Belongs to the herpesviridae tegument protein VP16 protein family.

Sequence caution

The sequence AAA45766.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processHost-virus interaction
Transcription
Transcription regulation
   Cellular componentHost nucleus
Virion
Virion tegument
   LigandDNA-binding
   PTMPhosphoprotein
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processregulation of transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

viral process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componenthost cell nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

viral tegument

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 490490Tegument protein VP16
PRO_0000115799

Regions

Region411 – 49080Transcriptional activation By similarity

Sites

Site4421Critical role in activation By similarity

Amino acid modifications

Modified residue181Phosphoserine By similarity
Modified residue3531Phosphoserine By similarity
Modified residue4111Phosphoserine By similarity
Modified residue4521Phosphoserine By similarity

Secondary structure

... 490
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P04486 [UniParc].

Last modified September 22, 2009. Version 2.
Checksum: 014372DE8FE6C417

FASTA49054,316
        10         20         30         40         50         60 
MDLLVDELFA DMDADGASPP PPRPAGGPKN TPAAPPLYAT GRLSQAQLMP SPPMPVPPAA 

        70         80         90        100        110        120 
LFNRLLDDLG FSAGPALCTM LDTWNEDLFS ALPTNADLYR ECKFLSTLPS DVVEWGDAYV 

       130        140        150        160        170        180 
PERAQIDIRA HGDVAFPTLP ATRDGLGLYY EALSRFFHAE LRAREESYRT VLANFCSALY 

       190        200        210        220        230        240 
RYLRASVRQL HRQAHMRGRD RDLGEMLRAT IADRYYRETA RLARVLFLHL YLFLTREILW 

       250        260        270        280        290        300 
AAYAEQMMRP DLFDCLCCDL ESWRQLAGLF QPFMFVNGAL TVRGVPIEAR RLRELNHIRE 

       310        320        330        340        350        360 
HLNLPLVRSA ATEEPGAPLT TPPTLHGNQA RASGYFMVLI RAKLDSYSSF TTSPSEAVMR 

       370        380        390        400        410        420 
EHAYSRARTK NNYGSTIEGL LDLPDDDAPE EAGLAAPRLS FLPAGHTRRL STAPPTDVSL 

       430        440        450        460        470        480 
GDELHLDGED VAMAHADALD DFDLDMLGDG DSPGPGFTPH DSAPYGALDM ADFEFEQMFT 

       490 
DALGIDEYGG 

« Hide

References

[1]"Nucleotide sequence and predicted amino acid sequence of a protein encoded in a small herpes simplex virus DNA fragment capable of trans-inducing alpha genes."
Pellett P.E., McKnight J.L.C., Jenkins F.J., Roizman B.
Proc. Natl. Acad. Sci. U.S.A. 82:5870-5874(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Combinatorial transcription of herpes simplex virus and varicella zoster virus immediate early genes is strictly determined by the cellular coactivator HCF-1."
Narayanan A., Nogueira M.L., Ruyechan W.T., Kristie T.M.
J. Biol. Chem. 280:1369-1375(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH HUMAN HCFC1.
[3]"Nuclear localizations of the herpes simplex virus type 1 tegument proteins VP13/14, vhs, and VP16 precede VP22-dependent microtubule reorganization and VP22 nuclear import."
Yedowitz J.C., Kotsakis A., Schlegel E.F., Blaho J.A.
J. Virol. 79:4730-4743(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[4]"Comprehensive characterization of extracellular herpes simplex virus type 1 virions."
Loret S., Guay G., Lippe R.
J. Virol. 82:8605-8618(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[5]"NMR structure of the complex between the Tfb1 subunit of TFIIH and the activation domain of VP16: structural similarities between VP16 and p53."
Langlois C., Mas C., Di Lello P., Jenkins L.M., Legault P., Omichinski J.G.
J. Am. Chem. Soc. 130:10596-10604(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 445-479 IN COMPLEX WITH THE TFB1 SUBUNIT OF TFIIH.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
K03350 Genomic DNA. Translation: AAA45766.1. Different initiation.
PIRIXBE1F. A03727.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2K2UNMR-B456-490[»]
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.1290.10. 1 hit.
InterProIPR003174. Alpha_TIF.
IPR021051. HSV_VP16_C.
[Graphical view]
PfamPF02232. Alpha_TIF. 1 hit.
PF12149. HSV_VP16_C. 1 hit.
[Graphical view]
SMARTSM00814. Alpha_TIF. 1 hit.
[Graphical view]
SUPFAMSSF56548. SSF56548. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP04486.

Entry information

Entry nameVP16_HHV1F
AccessionPrimary (citable) accession number: P04486
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: September 22, 2009
Last modified: February 19, 2014
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references