P04485 (ICP22_HHV11) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional regulator ICP22

Alternative name(s):
Immediate-early protein IE68
Infected cell protein 22
Short name=ICP22

Gene names

ORF Names:

US1

Organism

Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) [Reference proteome]

Taxonomic identifier

10299 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Simplexvirus › Human herpesvirus 1

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions as a general transcriptional regulator of cellular and viral mRNAs mainly by mediating changes on the host RNA polymerase II. One change, which is UL13 independent, is the rapid loss of Pol II forms bearing Ser-2 phosphorylation. A second change, which is UL13 dependent, is the appearance of an intermediate form of Pol II that differs from the normal hypo- and hyperphosphorylated forms. These Pol II modifications immediately inhibit host genome transcription, leading to cell cycle deregulation and loss of efficient antiviral response. Ref.5 Ref.6
Subcellular location	Host nucleus. Note: Localizes in small nuclear bodies early in infection then moves to a more diffuse distribution in viral compartments as infecion progresses.
Post-translational modification	Tyrosine phosphorylated. Ref.3
Miscellaneous	ICP22 and protein US1.5 mRNAs are transcribed from two different promoters on the US1 gene.
Sequence similarities	Belongs to the herpesviridae IE68 family.

Ontologies

Keywords
Biological process	Host gene expression shutoff by virus Host transcription shutoff by virus Host-virus interaction Inhibition of host RNA polymerase II by virus Transcription Transcription regulation
Cellular component	Host nucleus
Coding sequence diversity	Alternative promoter usage
Developmental stage	Early protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host RNA polymerase II activity Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative promoter usage. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform ICP22 (identifier: P04485-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform US1.5 (identifier: P04485-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-146: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	Transcriptional regulator ICP22		PRO_0000115837
Amino acid modifications
	Modified residue	193	1	Phosphotyrosine Probable
Natural variations
	Alternative sequence	1 – 146	146	Missing in isoform US1.5.		VSP_025673
Experimental info
	Mutagenesis	193	1	Y → A: Alteration of post-translational modifications. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform ICP22 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: 94D56376545B1192	FASTA	420	46,525
	10 20 30 40 50 60 MADISPGAFA PCVKARRPAL RSPPLGTRKR KRPSRPLSSE SEVESDTALE SEVESETASD 70 80 90 100 110 120 STESGDQDEA PRIGGRRAPR RLGGRFFLDM SAESTTGTET DASVSDDPDD TSDWSYDDIP 130 140 150 160 170 180 PRPKRARVNL RLTSSPDRRD GVIFPKMGRV RSTRETQPRA PTPSAPSPNA MLRRSVRQAQ 190 200 210 220 230 240 RRSSARWTPD LGYMRQCINQ LFRVLRVARD PHGSANRLRH LIRDCYLMGY CRARLAPRTW 250 260 270 280 290 300 CRLLQVSGGT WGMHLRNTIR EVEARFDATA EPVCKLPCLE TRRYGPECDL SNLEIHLSAT 310 320 330 340 350 360 SDDEISDATD LEAAGSDHTL ASQSDTEDAP SPVTLETPEP RGSLAVRLED EFGEFDWTPQ 370 380 390 400 410 420 EGSQPWLSAV VADTSSVERP GPSDSGAGRA AEDRKCLDGC RKMRFSTACP YPCSDTFLRP
	Isoform US1.5 [UniParc]. Checksum: 0C6802604B01100E	FASTA	274	30,580
	10 20 30 40 50 60 MGRVRSTRET QPRAPTPSAP SPNAMLRRSV RQAQRRSSAR WTPDLGYMRQ CINQLFRVLR 70 80 90 100 110 120 VARDPHGSAN RLRHLIRDCY LMGYCRARLA PRTWCRLLQV SGGTWGMHLR NTIREVEARF 130 140 150 160 170 180 DATAEPVCKL PCLETRRYGP ECDLSNLEIH LSATSDDEIS DATDLEAAGS DHTLASQSDT 190 200 210 220 230 240 EDAPSPVTLE TPEPRGSLAV RLEDEFGEFD WTPQEGSQPW LSAVVADTSS VERPGPSDSG 250 260 270 AGRAAEDRKC LDGCRKMRFS TACPYPCSDT FLRP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1." McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P. J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence determination and genetic content of the short unique region in the genome of herpes simplex virus type 1." McGeoch D.J., Dolan A., Donald S., Rixon F.J. J. Mol. Biol. 181:1-13(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Mutation of the protein tyrosine kinase consensus site in the herpes simplex virus 1 alpha22 gene alters ICP22 posttranslational modification." O'Toole J.M., Aubert M., Kotsakis A., Blaho J.A. Virology 305:153-167(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT TYR-193, MUTAGENESIS OF TYR-193.
[4]	"Herpes simplex virus 1 ICP22 regulates the accumulation of a shorter mRNA and of a truncated US3 protein kinase that exhibits altered functions." Poon A.P., Roizman B. J. Virol. 79:8470-8479(2005) [PubMed] [Europe PMC] [Abstract] Cited for: ISOFORM US1.5.
[5]	"The products of the herpes simplex virus type 1 immediate-early US1/US1.5 genes downregulate levels of S-phase-specific cyclins and facilitate virus replication in S-phase Vero cells." Orlando J.S., Astor T.L., Rundle S.A., Schaffer P.A. J. Virol. 80:4005-4016(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Herpes simplex virus immediate-early protein ICP22 triggers loss of serine 2-phosphorylated RNA polymerase II." Fraser K.A., Rice S.A. J. Virol. 81:5091-5101(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X14112 Genomic DNA. Translation: CAA32287.1. X02138 Genomic DNA. Translation: CAA26055.1. L00036 Genomic DNA. Translation: AAA96687.1.
PIR	EDBE17. A03723.
RefSeq	NP_044663.1. NC_001806.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-6732597.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2703435.
Phylogenomic databases
ProtClustDB	CLSP2510175.
Family and domain databases
InterPro	IPR003403. IE68. [Graphical view]
Pfam	PF02479. Herpes_IE68. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ICP22_HHV11

Accession

Primary (citable) accession number: P04485

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	April 3, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program