P04485 (ICP22_HHV11) Reviewed, UniProtKB/Swiss-Prot
Last modified October 16, 2013. Version 64. History...
Names and origin
|Protein names||Recommended name:|
Transcriptional regulator ICP22
Immediate-early protein IE68
Infected cell protein 22
|Organism||Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) [Reference proteome]|
|Taxonomic identifier||10299 [NCBI]|
|Taxonomic lineage||Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Simplexvirus ›|
|Virus host||Homo sapiens (Human) [TaxID: 9606]|
|Sequence length||420 AA.|
|Protein existence||Evidence at protein level|
General annotation (Comments)
Functions as a general transcriptional regulator of cellular and viral mRNAs mainly by mediating changes on the host RNA polymerase II. One change, which is UL13 independent, is the rapid loss of Pol II forms bearing Ser-2 phosphorylation. A second change, which is UL13 dependent, is the appearance of an intermediate form of Pol II that differs from the normal hypo- and hyperphosphorylated forms. These Pol II modifications immediately inhibit host genome transcription, leading to cell cycle deregulation and loss of efficient antiviral response. Ref.5 Ref.6
Host nucleus. Note: Localizes in small nuclear bodies early in infection then moves to a more diffuse distribution in viral compartments as infecion progresses.
Tyrosine phosphorylated. Ref.3
ICP22 and protein US1.5 mRNAs are transcribed from two different promoters on the US1 gene.
Belongs to the herpesviridae ICP22 family.
|Biological process||Host gene expression shutoff by virus|
Host transcription shutoff by virus
Inhibition of host RNA polymerase II by virus
|Cellular component||Host nucleus|
|Coding sequence diversity||Alternative promoter usage|
|Developmental stage||Early protein|
|Technical term||Complete proteome|
|Gene Ontology (GO)|
|Biological_process||suppression by virus of host RNA polymerase II activity|
Inferred from electronic annotation. Source: UniProtKB-KWtranscription, DNA-dependent
Inferred from electronic annotation. Source: UniProtKB-KW
|Cellular_component||host cell nucleus|
Inferred from electronic annotation. Source: UniProtKB-SubCell
|Complete GO annotation...|
|This entry describes 2 isoforms produced by alternative promoter usage. [Align] [Select]|
Note: Additional isoforms seem to exist.
|Isoform ICP22 (identifier: P04485-1) |
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
|Isoform US1.5 (identifier: P04485-2) |
The sequence of this isoform differs from the canonical sequence as follows:
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||1 – 420||420||Transcriptional regulator ICP22||PRO_0000115837|
Amino acid modifications
|Modified residue||193||1||Phosphotyrosine Probable|
|Alternative sequence||1 – 146||146||Missing in isoform US1.5.||VSP_025673|
|Mutagenesis||193||1||Y → A: Alteration of post-translational modifications. Ref.3|
|||"The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1."|
McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.
J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
|||"Sequence determination and genetic content of the short unique region in the genome of herpes simplex virus type 1."|
McGeoch D.J., Dolan A., Donald S., Rixon F.J.
J. Mol. Biol. 181:1-13(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
|||"Mutation of the protein tyrosine kinase consensus site in the herpes simplex virus 1 alpha22 gene alters ICP22 posttranslational modification."|
O'Toole J.M., Aubert M., Kotsakis A., Blaho J.A.
Virology 305:153-167(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT TYR-193, MUTAGENESIS OF TYR-193.
|||"Herpes simplex virus 1 ICP22 regulates the accumulation of a shorter mRNA and of a truncated US3 protein kinase that exhibits altered functions."|
Poon A.P., Roizman B.
J. Virol. 79:8470-8479(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: ISOFORM US1.5.
|||"The products of the herpes simplex virus type 1 immediate-early US1/US1.5 genes downregulate levels of S-phase-specific cyclins and facilitate virus replication in S-phase Vero cells."|
Orlando J.S., Astor T.L., Rundle S.A., Schaffer P.A.
J. Virol. 80:4005-4016(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
|||"Herpes simplex virus immediate-early protein ICP22 triggers loss of serine 2-phosphorylated RNA polymerase II."|
Fraser K.A., Rice S.A.
J. Virol. 81:5091-5101(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
|+||Additional computationally mapped references.|
|X14112 Genomic DNA. Translation: CAA32287.1.|
X02138 Genomic DNA. Translation: CAA26055.1.
L00036 Genomic DNA. Translation: AAA96687.1.
|PIR||EDBE17. A03723. |
|RefSeq||NP_044663.1. NC_001806.1. |
3D structure databases
Protein-protein interaction databases
|IntAct||P04485. 1 interaction.|
Protocols and materials databases
Genome annotation databases
Family and domain databases
|InterPro||IPR003403. IE68. |
|Pfam||PF02479. Herpes_IE68. 1 hit. |
|Accession||Primary (citable) accession number: P04485|
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Viral Protein Annotation Program|
Index of protein domains and families