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P04485 (ICP22_HHV11) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcriptional regulator ICP22
Alternative name(s):
Immediate-early protein IE68
Infected cell protein 22
Short name=ICP22
Gene names
ORF Names:US1
OrganismHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) [Reference proteome]
Taxonomic identifier10299 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length420 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as a general transcriptional regulator of cellular and viral mRNAs mainly by mediating changes on the host RNA polymerase II. One change, which is UL13 independent, is the rapid loss of Pol II forms bearing Ser-2 phosphorylation. A second change, which is UL13 dependent, is the appearance of an intermediate form of Pol II that differs from the normal hypo- and hyperphosphorylated forms. These Pol II modifications immediately inhibit host genome transcription, leading to cell cycle deregulation and loss of efficient antiviral response. Ref.5 Ref.6

Subcellular location

Host nucleus. Note: Localizes in small nuclear bodies early in infection then moves to a more diffuse distribution in viral compartments as infecion progresses.

Post-translational modification

Tyrosine phosphorylated. Ref.3

Miscellaneous

ICP22 and protein US1.5 mRNAs are transcribed from two different promoters on the US1 gene.

Sequence similarities

Belongs to the herpesviridae ICP22 family.

Alternative products

This entry describes 2 isoforms produced by alternative promoter usage. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform ICP22 (identifier: P04485-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform US1.5 (identifier: P04485-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-146: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 420420Transcriptional regulator ICP22
PRO_0000115837

Amino acid modifications

Modified residue1931Phosphotyrosine Probable

Natural variations

Alternative sequence1 – 146146Missing in isoform US1.5.
VSP_025673

Experimental info

Mutagenesis1931Y → A: Alteration of post-translational modifications. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform ICP22 [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: 94D56376545B1192

FASTA42046,525
        10         20         30         40         50         60 
MADISPGAFA PCVKARRPAL RSPPLGTRKR KRPSRPLSSE SEVESDTALE SEVESETASD 

        70         80         90        100        110        120 
STESGDQDEA PRIGGRRAPR RLGGRFFLDM SAESTTGTET DASVSDDPDD TSDWSYDDIP 

       130        140        150        160        170        180 
PRPKRARVNL RLTSSPDRRD GVIFPKMGRV RSTRETQPRA PTPSAPSPNA MLRRSVRQAQ 

       190        200        210        220        230        240 
RRSSARWTPD LGYMRQCINQ LFRVLRVARD PHGSANRLRH LIRDCYLMGY CRARLAPRTW 

       250        260        270        280        290        300 
CRLLQVSGGT WGMHLRNTIR EVEARFDATA EPVCKLPCLE TRRYGPECDL SNLEIHLSAT 

       310        320        330        340        350        360 
SDDEISDATD LEAAGSDHTL ASQSDTEDAP SPVTLETPEP RGSLAVRLED EFGEFDWTPQ 

       370        380        390        400        410        420 
EGSQPWLSAV VADTSSVERP GPSDSGAGRA AEDRKCLDGC RKMRFSTACP YPCSDTFLRP 

« Hide

Isoform US1.5 [UniParc].

Checksum: 0C6802604B01100E
Show »

FASTA27430,580

References

« Hide 'large scale' references
[1]"The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1."
McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.
J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequence determination and genetic content of the short unique region in the genome of herpes simplex virus type 1."
McGeoch D.J., Dolan A., Donald S., Rixon F.J.
J. Mol. Biol. 181:1-13(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Mutation of the protein tyrosine kinase consensus site in the herpes simplex virus 1 alpha22 gene alters ICP22 posttranslational modification."
O'Toole J.M., Aubert M., Kotsakis A., Blaho J.A.
Virology 305:153-167(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT TYR-193, MUTAGENESIS OF TYR-193.
[4]"Herpes simplex virus 1 ICP22 regulates the accumulation of a shorter mRNA and of a truncated US3 protein kinase that exhibits altered functions."
Poon A.P., Roizman B.
J. Virol. 79:8470-8479(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: ISOFORM US1.5.
[5]"The products of the herpes simplex virus type 1 immediate-early US1/US1.5 genes downregulate levels of S-phase-specific cyclins and facilitate virus replication in S-phase Vero cells."
Orlando J.S., Astor T.L., Rundle S.A., Schaffer P.A.
J. Virol. 80:4005-4016(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Herpes simplex virus immediate-early protein ICP22 triggers loss of serine 2-phosphorylated RNA polymerase II."
Fraser K.A., Rice S.A.
J. Virol. 81:5091-5101(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X14112 Genomic DNA. Translation: CAA32287.1.
X02138 Genomic DNA. Translation: CAA26055.1.
L00036 Genomic DNA. Translation: AAA96687.1.
PIREDBE17. A03723.
RefSeqNP_044663.1. NC_001806.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP04485. 1 interaction.
MINTMINT-6732597.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2703435.

Family and domain databases

InterProIPR003403. IE68.
[Graphical view]
PfamPF02479. Herpes_IE68. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameICP22_HHV11
AccessionPrimary (citable) accession number: P04485
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: July 9, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families