P04483 (TETR2_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tetracycline repressor protein class B from transposon Tn10

Gene names

Name:

tetR

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to tetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.
Sequence similarities	Contains 1 HTH tetR-type DNA-binding domain.

Ontologies

Keywords
Biological process	Antibiotic resistance Transcription Transcription regulation
Ligand	DNA-binding Magnesium Metal-binding
Molecular function	Repressor
Technical term	3D-structure Transposable element
Gene Ontology (GO)
Biological process	negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 207

207

Tetracycline repressor protein class B from transposon Tn10

PRO_0000070613

Regions

Domain

3 – 63

61

HTH tetR-type

DNA binding

26 – 45

20

H-T-H motif Potential

Sites

Metal binding

100

1

Magnesium [Mg-tetracycline] By similarity

Site

64

1

Involved in binding magnesium-tetracycline complex By similarity

Experimental info

Mutagenesis

64

1

H → Y: 1000-fold reduction of affinity for tetracycline.

Mutagenesis

82

1

N → H: 100-fold reduction of affinity for tetracycline.

Mutagenesis

103

1

T → I: <10-fold reduction of affinity for tetracycline.

Secondary structure

1

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.

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207

Helix Strand Turn

Details...

Helix

4 – 6

3

Helix

9 – 24

16

Helix

27 – 34

8

Helix

38 – 44

7

Helix

48 – 63

16

Helix

75 – 91

17

Helix

96 – 100

5

Helix

107 – 122

16

Helix

127 – 154

28

Helix

168 – 180

13

Helix

183 – 191

9

Sequences

	Sequence		Length	Mass (Da)
	P04483 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: A9FD75972352CC80	FASTA	207	23,355
	10 20 30 40 50 60 MSRLDKSKVI NSALELLNEV GIEGLTTRKL AQKLGVEQPT LYWHVKNKRA LLDALAIEML 70 80 90 100 110 120 DRHHTHFCPL EGESWQDFLR NNAKSFRCAL LSHRDGAKVH LGTRPTEKQY ETLENQLAFL 130 140 150 160 170 180 CQQGFSLENA LYALSAVGHF TLGCVLEDQE HQVAKEERET PTTDSMPPLL RQAIELFDHQ 190 200 GAEPAFLFGL ELIICGLEKQ LKCESGS

References

[1]	"Nucleotide sequence of the repressor gene of the TN10 tetracycline resistance determinant." Postle K., Nguyen T.T., Bertrand K.P. Nucleic Acids Res. 12:4849-4863(1984) [PubMed: 6330687] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A threonine to alanine exchange at position 40 of Tet repressor alters the recognition of the sixth base pair of tet operator from GC to AT." Altschmied L., Baumeister R., Pfleiderer K., Hillen W. EMBO J. 7:4011-4017(1988) [PubMed: 3208760] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-100.
[3]	"Overlapping divergent promoters control expression of Tn10 tetracycline resistance." Bertrand K.P., Postle K., Wray L.V. Jr., Reznikoff W.S. Gene 23:149-156(1983) [PubMed: 6311683] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-22.
[4]	"Mutations in the Tn10 tet repressor that interfere with induction. Location of the tetracycline-binding domain." Smith L.D., Bertrand K.P. J. Mol. Biol. 203:949-959(1988) [PubMed: 3062183] [Abstract] Cited for: MUTAGENESIS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X00694 Genomic DNA. Translation: CAA25291.1.
J01830 Genomic DNA. Translation: AAB59093.1.

PIR

RPECTN. A03576.

RefSeq

YP_001096449.1. NC_009133.1.
YP_002456171.1. NC_011812.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2NS7	X-ray	2.40	A/B/C/D	1-187	[»]
2NS8	X-ray	2.55	A/B/C/D	1-187	[»]
2VKV	X-ray	1.74	A	1-50	[»]
2XGC	X-ray	2.15	A/B	1-50	[»]
2XGD	X-ray	2.25	A	1-50	[»]
2XGE	X-ray	2.14	A/B	1-50	[»]
3FK6	X-ray	2.10	A/B	1-12	[»]
3FK7	X-ray	2.06	A/B	1-12	[»]

ProteinModelPortal

P04483.

SMR

P04483. Positions 2-201.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

4924774.
7256840.

Phylogenomic databases

ProtClustDB

PRK13756.

Family and domain databases

InterPro

IPR001647. DNA-bd_HTH_TetR-type.
IPR023772. DNA-bd_HTH_TetR-type_CS.
IPR009057. Homeodomain-like.
IPR012287. Homeodomain-rel.
IPR003012. Tet_transcr_reg_TetR.
IPR015893. Tet_transcr_reg_TetR-like_C.
IPR011075. Tet_transcr_reg_TetR-rel_C.
IPR004111. Tet_transcr_reg_TetR_C.
IPR023773. Tscrpt_reg_HTH_TetR-type.
[Graphical view]

Gene3D

G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.
G3DSA:1.10.357.10. TetR_C. 1 hit.

Pfam

PF02909. TetR_C. 1 hit.
PF00440. TetR_N. 1 hit.
[Graphical view]

PRINTS

PR00455. HTHTETR.
PR00400. TETREPRESSOR.

SUPFAM

SSF46689. Homeodomain_like. 1 hit.
SSF48498. TetR_like_C. 1 hit.

PROSITE

PS01081. HTH_TETR_1. 1 hit.
PS50977. HTH_TETR_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

TETR2_ECOLX

Accession

Primary (citable) accession number: P04483

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	June 28, 2011
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program