P04482 (IMM2_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Colicin-E2 immunity protein

Alternative name(s):
ImmE2
Microcin-E2 immunity protein

Gene names

Name:	imm
Synonyms:	ceiB

Encoded on

Plasmid ColE2-P9

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	86 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protein is able to protect a cell, which harbors the plasmid ColE2 encoding colicin E2, against colicin E2.
Sequence similarities	Belongs to the colicins ColE2/ColE8/ColE9 and pyocins S1/S2 family.

Ontologies

Keywords
Biological process	Bacteriocin immunity
Technical term	3D-structure Plasmid
Gene Ontology (GO)
Biological_process	bacteriocin immunity Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	toxic substance binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 86

86

Colicin-E2 immunity protein

PRO_0000218703

Secondary structure

1

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86

Helix Strand Turn

Details...

Helix

7 – 9

3

Helix

12 – 24

13

Beta strand

27 – 29

3

Helix

30 – 44

15

Turn

47 – 50

4

Helix

51 – 54

4

Beta strand

58 – 60

3

Helix

64 – 77

14

Sequences

	Sequence		Length	Mass (Da)
	P04482 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: 1C5C89ED7E87BC39	FASTA	86	9,995
	10 20 30 40 50 60 MELKHSISDY TEAEFLEFVK KICRAEGATE EDDNKLVREF ERLTEHPDGS DLIYYPRDDR 70 80 EDSPEGIVKE IKEWRAANGK SGFKQG

References

[1]	"Comparative nucleotide sequences encoding the immunity proteins and the carboxyl-terminal peptides of colicins E2 and E3." Lau P.C.K., Rowsome R.W., Zuker M., Visentin L.P. Nucleic Acids Res. 12:8733-8745(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Structure and expression of the ColE2-P9 immunity gene." Masaki H., Toba M., Ohta T. Nucleic Acids Res. 13:1623-1635(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Molecular characterisation of the colicin E2 operon and identification of its products." Cole S.T., Saint-Joanis B., Pugsley A.P. Mol. Gen. Genet. 198:465-472(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X02227 Genomic DNA. Translation: CAA26146.1.
X01163 Genomic DNA. Translation: CAA25610.1.
M29885 Genomic DNA. Translation: AAA23069.1.

PIR

IMECE2. A03512.

RefSeq

YP_001966153.1. NC_010883.1.
YP_002221665.1. NC_011228.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2NO8	NMR	-	A	1-86	[»]
2WPT	X-ray	1.78	A	1-86	[»]
3U43	X-ray	1.72	A	1-86	[»]

ProteinModelPortal

P04482.

SMR

P04482. Positions 2-86.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-59336N.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

6383670.
6921858.

Phylogenomic databases

ProtClustDB

CLSK2311388.

Family and domain databases

Gene3D

1.10.1200.20. 1 hit.

InterPro

IPR023802. Colicin/pyocin_immunity_dom.
IPR000290. Colicin_pyocin.
[Graphical view]

Pfam

PF01320. Colicin_Pyocin. 1 hit.
[Graphical view]

PRINTS

PR01299. PYOCIN.

ProDom

PD007225. Colicin_pyocin. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF47345. Colicin_pyocin. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P04482.

Entry information

Entry name

IMM2_ECOLX

Accession

Primary (citable) accession number: P04482

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	May 29, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program