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Protein

Colicin-A

Gene

caa

Organism
Citrobacter freundii
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This colicin is a channel-forming colicin. This class of transmembrane toxins depolarize the cytoplasmic membrane, leading to dissipation of cellular energy.
Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Bacteriocin

Protein family/group databases

TCDBi1.C.1.3.1. the channel-forming colicin (colicin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Colicin-A
Gene namesi
Name:caa
Encoded oniPlasmid ColA-CA310 Publication
OrganismiCitrobacter freundii
Taxonomic identifieri546 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCitrobacterCitrobacter freundii complex

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei528 – 54821HelicalSequence analysisAdd
BLAST
Transmembranei555 – 57521HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 592592Colicin-APRO_0000218666Add
BLAST

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
tolAP199346EBI-6559916,EBI-1120026From a different organism.

Protein-protein interaction databases

IntActiP04480. 2 interactions.
MINTiMINT-7888500.

Structurei

Secondary structure

1
592
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi14 – 163Combined sources
Beta strandi58 – 603Combined sources
Beta strandi63 – 664Combined sources
Turni82 – 843Combined sources
Beta strandi86 – 905Combined sources
Beta strandi93 – 975Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3IAXX-ray2.60B1-107[»]
3QDRX-ray2.65B53-107[»]
ProteinModelPortaliP04480.
SMRiP04480. Positions 393-589.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04480.

Family & Domainsi

Sequence similaritiesi

Belongs to the channel forming colicin family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.10.490.30. 1 hit.
InterProiIPR000293. Channel_colicin_C.
[Graphical view]
PfamiPF01024. Colicin. 1 hit.
[Graphical view]
PRINTSiPR00280. CHANLCOLICIN.
ProDomiPD002657. Channel_colicin_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS00276. CHANNEL_COLICIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04480-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGFNYGGKG DGTGWSSERG SGPEPGGGSH GNSGGHDRGD SSNVGNESVT
60 70 80 90 100
VMKPGDSYNT PWGKVIINAA GQPTMNGTVM TADNSSMVPY GRGFTRVLNS
110 120 130 140 150
LVNNPVSPAG QNGGKSPVQT AVENYLMVQS GNLPPGYWLS NGKVMTEVRE
160 170 180 190 200
ERTSGGGGKN GNERTWTVKV PREVPQLTAS YNEGMRIRQE AADRARAEAN
210 220 230 240 250
ARALAEEEAR AIASGKSKAE FDAGKRVEAA QAAINTAQLN VNNLSGAVSA
260 270 280 290 300
ANQVITQKQA EMTPLKNELA AANQRVQETL KFINDPIRSR IHFNMRSGLI
310 320 330 340 350
RAQHNVDTKQ NEINAAVANR DALNSQLSQA NNILQNARNE KSAADAALSA
360 370 380 390 400
ATAQRLQAEA ALRAAAEAAE KARQRQAEEA ERQRQAMEVA EKAKDERELL
410 420 430 440 450
EKTSELIAGM GDKIGEHLGD KYKAIAKDIA DNIKNFQGKT IRSFDDAMAS
460 470 480 490 500
LNKITANPAM KINKADRDAL VNAWKHVDAQ DMANKLGNLS KAFKVADVVM
510 520 530 540 550
KVEKVREKSI EGYETGNWGP LMLEVESWVL SGIASSVALG IFSATLGAYA
560 570 580 590
LSLGVPAIAV GIAGILLAAV VGALIDDKFA DALNNEIIRP AH
Length:592
Mass (Da):62,993
Last modified:August 13, 1987 - v1
Checksum:iB80FA1F52A8CFC5D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01008 Genomic DNA. Translation: CAA25503.1.
M37402 Genomic DNA. Translation: AAA72879.1.
M26369 Genomic DNA. Translation: AAA98057.1.
PIRiI40784. IKEBCA.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01008 Genomic DNA. Translation: CAA25503.1.
M37402 Genomic DNA. Translation: AAA72879.1.
M26369 Genomic DNA. Translation: AAA98057.1.
PIRiI40784. IKEBCA.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3IAXX-ray2.60B1-107[»]
3QDRX-ray2.65B53-107[»]
ProteinModelPortaliP04480.
SMRiP04480. Positions 393-589.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP04480. 2 interactions.
MINTiMINT-7888500.

Protein family/group databases

TCDBi1.C.1.3.1. the channel-forming colicin (colicin) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP04480.

Family and domain databases

Gene3Di1.10.490.30. 1 hit.
InterProiIPR000293. Channel_colicin_C.
[Graphical view]
PfamiPF01024. Colicin. 1 hit.
[Graphical view]
PRINTSiPR00280. CHANLCOLICIN.
ProDomiPD002657. Channel_colicin_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS00276. CHANNEL_COLICIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCEA_CITFR
AccessioniPrimary (citable) accession number: P04480
Secondary accession number(s): Q51599
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: November 11, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.