P04464 (CALM_WHEAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Calmodulin Short name=CaM
Organism	Triticum aestivum (Wheat)
Taxonomic identifier	4565 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Triticum

Protein attributes

Sequence length	149 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Calmodulin mediates the control of a large number of enzymes, ion channels and other proteins by Ca2+. Among the enzymes to be stimulated by the calmodulin-Ca2+ complex are a number of protein kinases and phosphatases.
Miscellaneous	This protein has four functional calcium-binding sites.
Sequence similarities	Belongs to the calmodulin family. Contains 4 EF-hand domains.

Ontologies

Keywords
Domain	Repeat
Ligand	Calcium
PTM	Acetylation Methylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 149	148	Calmodulin		PRO_0000198308
Regions
	Domain	8 – 43	36	EF-hand 1
	Domain	44 – 79	36	EF-hand 2
	Domain	81 – 116	36	EF-hand 3
	Domain	117 – 149	33	EF-hand 4
	Calcium binding	21 – 32	12	1
	Calcium binding	57 – 68	12	2
	Calcium binding	94 – 105	12	3
	Calcium binding	130 – 141	12	4
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.1
	Modified residue	116	1	N6,N6,N6-trimethyllysine Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P04464 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 9E5AF05D066A759C	FASTA	149	16,847
	10 20 30 40 50 60 MADQLTDEQI AEFKEAFSLF DKDGDGCITT KELGTVMRSL GQNPTEAELQ DMINEVDADG 70 80 90 100 110 120 NGTIDFPEFL NLMARKMKDT DSEEELKEAF RVFDKDQDGF ISAAELRHVM TNLGEKLTDE 130 140 EVDEMIREAD VDGDGQINYE EFVKVMMAK

References

[1]	"Amino acid sequence of calmodulin from wheat germ." Toda H., Yazawa M., Sakiyama F., Yagi K. J. Biochem. 98:833-842(1985) [PubMed: 4086472] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-149. Tissue: Germ.
[2]	Erratum Toda H., Yazawa M., Sakiyama F., Yagi K. J. Biochem. 115:367-367(1994) [PubMed: 8206888] [Abstract] Cited for: SEQUENCE REVISION.

Cross-references

Sequence databases
PIR	MCWT. A03025.
3D structure databases
ProteinModelPortal	P04464.
SMR	P04464. Positions 1-148.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	P04464.
Family and domain databases
InterPro	IPR018248. EF-hand. IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca-bd. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 2 hits.
Pfam	PF00036. efhand. 4 hits. [Graphical view]
SMART	SM00054. EFh. 4 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 4 hits. PS50222. EF_HAND_2. 4 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CALM_WHEAT

Accession

Primary (citable) accession number: P04464

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	January 23, 2007
Last modified:	September 21, 2011
This is version 69 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program