Reviewed, UniProtKB/Swiss-Prot P04451 (RL23_YEAST)

Names and origin

Protein names

Recommended name:
    60S ribosomal protein L23
Alternative name(s):
    L17a
    YL32

Gene names

Name:	RPL23A
Synonyms:	RPL17A, RPL17AA
Ordered Locus Names:	YBL087C
ORF Names:	YBL0713
AND
Name:	RPL23B
Synonyms:	RPL17AB, RPL17B
Ordered Locus Names:	YER117W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	137 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subunit structure	Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.7
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Methylated by RKM1 at 2 different sites, but it is unclear which are the 2 methylated residues among Lys-40, Lys-106 and/or Lys-110. Ref.8
Miscellaneous	There are 2 genes for L23 in yeast.
Sequence similarities	Belongs to the ribosomal protein L14P family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation Methylation Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	response to drug Inferred from mutant phenotype. Source: SGD translation Ref.7 Traceable author statement. Source: SGD
Cellular component	cytosolic large ribosomal subunit Ref.7 Traceable author statement. Source: SGD
Molecular function	structural constituent of ribosome Ref.7 Traceable author statement. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 137	136	60S ribosomal protein L23		PRO_0000128630
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.8
	Modified residue	40	1	N6,N6-dimethyllysine; by RKM1 Probable
	Modified residue	106	1	N6,N6-dimethyllysine; by RKM1 Probable
	Modified residue	110	1	N6,N6-dimethyllysine; by RKM1 Probable
	Modified residue	112	1	Phosphoserine Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P04451-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: DEB983B3CB1DFAB1	FASTA	137	14,473
	10 20 30 40 50 60 MSGNGAQGTK FRISLGLPVG AIMNCADNSG ARNLYIIAVK GSGSRLNRLP AASLGDMVMA 70 80 90 100 110 120 TVKKGKPELR KKVMPAIVVR QAKSWRRRDG VFLYFEDNAG VIANPKGEMK GSAITGPVGK 130 ECADLWPRVA SNSGVVV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural comparison of yeast ribosomal protein genes." Leer R.J., van Raamsdonk-Duin M.M.C., Hagendoorn M.J.M., Mager W.H., Planta R.J. Nucleic Acids Res. 12:6685-6700(1984) [PubMed: 6091033] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (RPL23A).
[2]	"Sequence, map position and genome organization of the RPL17B gene, encoding ribosomal protein L17b in Saccharomyces cerevisiae." Berroteran R.W., Hampsey M. Yeast 11:761-766(1995) [PubMed: 7668045] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (RPL23B).
[3]	Erratum Berroteran R.W., Hampsey M. Yeast 12:91-91(1996)
[4]	"Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II." Obermaier B., Gassenhuber J., Piravandi E., Domdey H. Yeast 11:1103-1112(1995) [PubMed: 7502586] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (RPL23A). Strain: ATCC 204508 / S288c.
[5]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (RPL23A). Strain: ATCC 204508 / S288c.
[6]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (RPL23B). Strain: ATCC 204511 / S288c / AB972.
[7]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed: 9559554] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[8]	"A novel SET domain methyltransferase modifies ribosomal protein Rpl23ab in yeast." Porras-Yakushi T.R., Whitelegge J.P., Miranda T.B., Clarke S. J. Biol. Chem. 280:34590-34598(2005) [PubMed: 16096273] [Abstract] Cited for: ACETYLATION AT SER-2, METHYLATION AT LYS-40; LYS-106 AND LYS-110.
[9]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-112, MASS SPECTROMETRY.
[10]	"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions." Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J. Cell 107:373-386(2001) [PubMed: 11701127] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 7-137, ELECTRON MICROSCOPY.
[11]	"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation." Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J. EMBO J. 23:1008-1019(2004) [PubMed: 14976550] [Abstract] Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X01694 Genomic DNA. Translation: CAA25841.1.
U15653 Genomic DNA. Translation: AAA61906.1.
X79489 Genomic DNA. Translation: CAA56018.1.
Z35848 Genomic DNA. Translation: CAA84908.1.
U18916 Genomic DNA. Translation: AAC03215.1.

PIR

R5BY17. A02792.

RefSeq

NP_009466.1.
NP_011042.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K5Y	model	-	R	7-137	[»]
1S1I	electron microscopy	11.70	R	1-137	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-5601N.

IntAct

P04451. 48 interactions.

STRING

P04451.

Proteomic databases

PRIDE

P04451.

Genome annotation databases

Ensembl

YBL087C; YBL087C; YBL087C; Saccharomyces cerevisiae. [Genome view]
YER117W; YER117W; YER117W; Saccharomyces cerevisiae. [Genome view]

GeneID

852191.
856853.

KEGG

sce:YBL087C.
sce:YER117W.

Organism-specific databases

CYGD

YBL087c.
YER117w.

SGD

S000000183. RPL23A.
S000000919. RPL23B.

Phylogenomic databases

HOGENOM

HBG594899.

OMA

TPEMRKQ.

OrthoDB

EOG9MPK6S.

Gene expression databases

ArrayExpress

P04451.

Genevestigator

P04451.

GermOnline

YBL087C. Saccharomyces cerevisiae.
YER117W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR019972. Ribosomal_L14_CS.
IPR000218. Ribosomal_L14b/L23e.
[Graphical view]

Gene3D

G3DSA:2.40.150.20. Ribosomal_L14. 1 hit.

PANTHER

PTHR11761. Ribosomal_L14. 1 hit.

Pfam

PF00238. Ribosomal_L14. 1 hit.
[Graphical view]

PROSITE

PS00049. RIBOSOMAL_L14. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

970670.

Entry information

Entry name

RL23_YEAST

Accession

Primary (citable) accession number: P04451

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	January 23, 2007
Last modified:	December 15, 2009
This is version 92 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)