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Protein

50S ribosomal protein L14

Gene

rplN

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Forms part of two intersubunit bridges in the 70S ribosome (By similarity). Binds to 23S rRNA.By similarity

GO - Molecular functioni

  1. rRNA binding Source: UniProtKB-HAMAP
  2. structural constituent of ribosome Source: InterPro

GO - Biological processi

  1. translation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Ligandi

RNA-binding, rRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
50S ribosomal protein L14UniRule annotation
Gene namesi
Name:rplNUniRule annotation
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Subcellular locationi

GO - Cellular componenti

  1. large ribosomal subunit Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12212250S ribosomal protein L14PRO_0000128529Add
BLAST

Proteomic databases

PRIDEiP04450.

Interactioni

Subunit structurei

Forms a cluster with proteins L3 and L19. In the 70S ribosome, L14 and L19 interact and together make contacts with the 16S rRNA in bridges B5 and B8 (By similarity). Part of the 50S ribosomal subunit.By similarity

Structurei

Secondary structure

1
122
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 104Combined sources
Beta strandi12 – 2413Combined sources
Beta strandi38 – 469Combined sources
Beta strandi50 – 523Combined sources
Beta strandi57 – 648Combined sources
Beta strandi83 – 875Combined sources
Beta strandi93 – 964Combined sources
Helixi105 – 1084Combined sources
Helixi112 – 1176Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WHIX-ray1.50A1-122[»]
4V4TX-ray6.46O1-122[»]
SMRiP04450. Positions 1-122.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04450.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L14P family.UniRule annotation

Family and domain databases

Gene3Di2.40.150.20. 1 hit.
HAMAPiMF_01367. Ribosomal_L14.
InterProiIPR005745. Ribosomal_L14_bac-type.
IPR019972. Ribosomal_L14_CS.
IPR023571. Ribosomal_L14_dom.
IPR000218. Ribosomal_L14b/L23e.
[Graphical view]
PANTHERiPTHR11761. PTHR11761. 1 hit.
PfamiPF00238. Ribosomal_L14. 1 hit.
[Graphical view]
SUPFAMiSSF50193. SSF50193. 1 hit.
TIGRFAMsiTIGR01067. rplN_bact. 1 hit.
PROSITEiPS00049. RIBOSOMAL_L14. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04450-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIQQESRLKV ADNSGAREVL VIKVLGGSGR RYANIGDVVV ATVKDATPGG
60 70 80 90 100
VVKKGQVVKA VVVRTKRGVR RPDGSYIRFD ENACVIIRDD KSPRGTRIFG
110 120
PVARELRDKD FMKIISLAPE VI
Length:122
Mass (Da):13,346
Last modified:August 13, 1987 - v1
Checksum:iEF0BA6CD5927D324
GO

Sequence databases

PIRiA02789. R5BS14.

Cross-referencesi

Sequence databases

PIRiA02789. R5BS14.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WHIX-ray1.50A1-122[»]
4V4TX-ray6.46O1-122[»]
SMRiP04450. Positions 1-122.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP04450.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP04450.

Family and domain databases

Gene3Di2.40.150.20. 1 hit.
HAMAPiMF_01367. Ribosomal_L14.
InterProiIPR005745. Ribosomal_L14_bac-type.
IPR019972. Ribosomal_L14_CS.
IPR023571. Ribosomal_L14_dom.
IPR000218. Ribosomal_L14b/L23e.
[Graphical view]
PANTHERiPTHR11761. PTHR11761. 1 hit.
PfamiPF00238. Ribosomal_L14. 1 hit.
[Graphical view]
SUPFAMiSSF50193. SSF50193. 1 hit.
TIGRFAMsiTIGR01067. rplN_bact. 1 hit.
PROSITEiPS00049. RIBOSOMAL_L14. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The complete primary structure of ribosomal proteins L1, L14, L15, L23, L24 and L29 from Bacillus stearothermophilus."
    Kimura M., Kimura J., Ashman K.
    Eur. J. Biochem. 150:491-497(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
  2. "Protein-rRNA binding features and their structural and functional implications in ribosomes as determined by cross-linking studies."
    Urlaub H., Kruft V., Bischof O., Mueller E.-C., Wittmann-Liebold B.
    EMBO J. 14:4578-4588(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-35, CROSS-LINKING TO RRNA.
    Strain: 799.
  3. "The crystal structure of ribosomal protein L14 reveals an important organizational component of the translational apparatus."
    Davies C., White S.W., Ramakrishnan V.
    Structure 4:55-66(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).
  4. "Placement of protein and RNA structures into a 5 A-resolution map of the 50S ribosomal subunit."
    Ban N., Nissen P., Hansen J., Capel M., Moore P.B., Steitz T.A.
    Nature 400:841-847(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING ONTO THE H.MARISMORTUI 50S RIBOSOME.

Entry informationi

Entry nameiRL14_GEOSE
AccessioniPrimary (citable) accession number: P04450
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: April 1, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.