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P04450

- RL14_GEOSE

UniProt

P04450 - RL14_GEOSE

Protein

50S ribosomal protein L14

Gene

rplN

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Forms part of two intersubunit bridges in the 70S ribosome By similarity. Binds to 23S rRNA.By similarity

    GO - Molecular functioni

    1. rRNA binding Source: UniProtKB-HAMAP
    2. structural constituent of ribosome Source: InterPro

    GO - Biological processi

    1. translation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Keywords - Ligandi

    RNA-binding, rRNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    50S ribosomal protein L14UniRule annotation
    Gene namesi
    Name:rplNUniRule annotation
    OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
    Taxonomic identifieri1422 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

    Subcellular locationi

    GO - Cellular componenti

    1. large ribosomal subunit Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12212250S ribosomal protein L14PRO_0000128529Add
    BLAST

    Proteomic databases

    PRIDEiP04450.

    Interactioni

    Subunit structurei

    Forms a cluster with proteins L3 and L19. In the 70S ribosome, L14 and L19 interact and together make contacts with the 16S rRNA in bridges B5 and B8 By similarity. Part of the 50S ribosomal subunit.By similarity

    Structurei

    Secondary structure

    1
    122
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi7 – 104
    Beta strandi12 – 2413
    Beta strandi38 – 469
    Beta strandi50 – 523
    Beta strandi57 – 648
    Beta strandi83 – 875
    Beta strandi93 – 964
    Helixi105 – 1084
    Helixi112 – 1176

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1WHIX-ray1.50A1-122[»]
    2B9PX-ray6.46O1-122[»]
    ProteinModelPortaliP04450.
    SMRiP04450. Positions 1-122.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP04450.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L14P family.UniRule annotation

    Family and domain databases

    Gene3Di2.40.150.20. 1 hit.
    HAMAPiMF_01367. Ribosomal_L14.
    InterProiIPR005745. Ribosomal_L14_bac-type.
    IPR019972. Ribosomal_L14_CS.
    IPR023571. Ribosomal_L14_dom.
    IPR000218. Ribosomal_L14b/L23e.
    [Graphical view]
    PANTHERiPTHR11761. PTHR11761. 1 hit.
    PfamiPF00238. Ribosomal_L14. 1 hit.
    [Graphical view]
    SUPFAMiSSF50193. SSF50193. 1 hit.
    TIGRFAMsiTIGR01067. rplN_bact. 1 hit.
    PROSITEiPS00049. RIBOSOMAL_L14. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P04450-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIQQESRLKV ADNSGAREVL VIKVLGGSGR RYANIGDVVV ATVKDATPGG    50
    VVKKGQVVKA VVVRTKRGVR RPDGSYIRFD ENACVIIRDD KSPRGTRIFG 100
    PVARELRDKD FMKIISLAPE VI 122
    Length:122
    Mass (Da):13,346
    Last modified:August 13, 1987 - v1
    Checksum:iEF0BA6CD5927D324
    GO

    Sequence databases

    PIRiA02789. R5BS14.

    Cross-referencesi

    Sequence databases

    PIRi A02789. R5BS14.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1WHI X-ray 1.50 A 1-122 [» ]
    2B9P X-ray 6.46 O 1-122 [» ]
    ProteinModelPortali P04450.
    SMRi P04450. Positions 1-122.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P04450.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P04450.

    Family and domain databases

    Gene3Di 2.40.150.20. 1 hit.
    HAMAPi MF_01367. Ribosomal_L14.
    InterProi IPR005745. Ribosomal_L14_bac-type.
    IPR019972. Ribosomal_L14_CS.
    IPR023571. Ribosomal_L14_dom.
    IPR000218. Ribosomal_L14b/L23e.
    [Graphical view ]
    PANTHERi PTHR11761. PTHR11761. 1 hit.
    Pfami PF00238. Ribosomal_L14. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50193. SSF50193. 1 hit.
    TIGRFAMsi TIGR01067. rplN_bact. 1 hit.
    PROSITEi PS00049. RIBOSOMAL_L14. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete primary structure of ribosomal proteins L1, L14, L15, L23, L24 and L29 from Bacillus stearothermophilus."
      Kimura M., Kimura J., Ashman K.
      Eur. J. Biochem. 150:491-497(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE.
    2. "Protein-rRNA binding features and their structural and functional implications in ribosomes as determined by cross-linking studies."
      Urlaub H., Kruft V., Bischof O., Mueller E.-C., Wittmann-Liebold B.
      EMBO J. 14:4578-4588(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 24-35, CROSS-LINKING TO RRNA.
      Strain: 799.
    3. "The crystal structure of ribosomal protein L14 reveals an important organizational component of the translational apparatus."
      Davies C., White S.W., Ramakrishnan V.
      Structure 4:55-66(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).
    4. "Placement of protein and RNA structures into a 5 A-resolution map of the 50S ribosomal subunit."
      Ban N., Nissen P., Hansen J., Capel M., Moore P.B., Steitz T.A.
      Nature 400:841-847(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING ONTO THE H.MARISMORTUI 50S RIBOSOME.

    Entry informationi

    Entry nameiRL14_GEOSE
    AccessioniPrimary (citable) accession number: P04450
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: August 13, 1987
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3