60S ribosomal protein L24-A - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P04449 (RL24A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 120. History...

Clusters with 100%, 90%, 50% identity |

Documents (5) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
60S ribosomal protein L24-A

Alternative name(s):
L30
RP29
YL21

Gene names

Name:	RPL24A
Synonyms:	RP29, RPL30A
Ordered Locus Names:	YGL031C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	155 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.5
Subcellular location	Cytoplasm Ref.7.
Miscellaneous	Present with 247000 molecules/cell in log phase SD medium. There are 2 genes for L24 in yeast.
Sequence similarities	Belongs to the ribosomal protein L24e family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Traceable author statement Ref.5. Source: SGD
Cellular_component	cytosolic large ribosomal subunit Traceable author statement Ref.5. Source: SGD
Molecular_function	RNA binding Inferred from direct assay PubMed 6337137. Source: SGD structural constituent of ribosome Traceable author statement Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 155

155

60S ribosomal protein L24-A

PRO_0000136894

Amino acid modifications

Modified residue

Phosphoserine Ref.13

Modified residue

Phosphoserine Ref.11

Modified residue

Phosphothreonine Ref.13

Modified residue

Phosphoserine Ref.9 Ref.10 Ref.11 Ref.12 Ref.13

Modified residue

Phosphoserine Ref.11

Modified residue

154

Phosphoserine Ref.6

Sequences

Sequence

Length

Mass (Da)

Tools

P04449 [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: 9A79C4E409515716
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FASTA

155

17,614

        10         20         30         40         50         60 
MKVEIDSFSG AKIYPGRGTL FVRGDSKIFR FQNSKSASLF KQRKNPRRIA WTVLFRKHHK 

        70         80         90        100        110        120 
KGITEEVAKK RSRKTVKAQR PITGASLDLI KERRSLKPEV RKANREEKLK ANKEKKKAEK 

       130        140        150 
AARKAEKAKS AGTQSSKFSK QQAKGAFQKV AATSR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A yeast ribosomal protein gene whose intron is in the 5' leader." Mitra G., Warner J.R. J. Biol. Chem. 259:9218-9224(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing." Juneau K., Palm C., Miranda M., Davis R.W. Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-73. Strain: ATCC 201390 / BY4743.
[5]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[6]	"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-154, MASS SPECTROMETRY. Strain: 2124.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-86, MASS SPECTROMETRY. Strain: YAL6B.
[10]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-86, MASS SPECTROMETRY. Strain: ADR376.
[11]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26; SER-86 AND SER-95, MASS SPECTROMETRY.
[12]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-86, MASS SPECTROMETRY.
[13]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9; THR-83 AND SER-86, MASS SPECTROMETRY.
[14]	"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions." Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J. Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 4-56, ELECTRON MICROSCOPY.
[15]	"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation." Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J. EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 1-56, ELECTRON MICROSCOPY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

K02650 Genomic DNA. Translation: AAA35004.1.
Z72553 Genomic DNA. Translation: CAA96732.1.
EF123140 mRNA. Translation: ABM97484.1.
BK006941 Genomic DNA. Translation: DAA08068.1.

PIR

R6BYT9. A02783.

RefSeq

NP_011484.1. NM_001180896.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K5Y	model	-	S	4-56	[»]
1S1I	electron microscopy	11.70	S	1-56	[»]
2X7N	electron microscopy	11.80	D	1-56	[»]
3IZS	electron microscopy	-	Z	1-155	[»]
3O58	X-ray	4.00	V	1-155	[»]
3O5H	X-ray	4.00	V	1-155	[»]
3U5E	X-ray	3.00	W	1-155	[»]
3U5I	X-ray	3.00	W	1-155	[»]
4B6A	electron microscopy	8.10	W	1-155	[»]

ProteinModelPortal

P04449.

SMR

P04449. Positions 1-135.

ModBase

Search...

Protein-protein interaction databases

IntAct

P04449. 15 interactions.

MINT

MINT-1325572.

Proteomic databases

PeptideAtlas

P04449.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YGL031C; YGL031C; YGL031C.

GeneID

852852.

KEGG

sce:YGL031C.

Organism-specific databases

CYGD

YGL031c.

SGD

S000002999. RPL24A.

Phylogenomic databases

GeneTree

ENSGT00550000074809.

HOGENOM

HOG000184343.

K02896.

OMA

MRTYDDS.

OrthoDB

EOG4W11PR.

Gene expression databases

Genevestigator

P04449.

GermOnline

YGL031C. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

2.30.170.20. 1 hit.

InterPro

IPR023442. Ribosomal_L24e_CS.
IPR023441. Ribosomal_L24e_dom.
IPR000988. Ribosomal_L24e_rel.
[Graphical view]

PANTHER

PTHR10792. PTHR10792. 1 hit.

Pfam

PF01246. Ribosomal_L24e. 1 hit.
[Graphical view]

PROSITE

PS01073. RIBOSOMAL_L24E. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P04449.

NextBio

972446.

Entry information

Entry name

RL24A_YEAST

Accession

Primary (citable) accession number: P04449
Secondary accession number(s): A2TBN7, D6VUA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	April 3, 2013
This is version 120 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program