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Protein

Transcription factor 1

Gene

TF1

Organism
Bacillus phage SP01 (Bacteriophage SP01)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Selectively binds to and inhibits the transcription of hydroxymethyluracil-(hmUra)-containing DNA, such as SP01 DNA, by RNA polymerase in vitro.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi61 – 611
DNA bindingi93 – 942Sequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor 1
Gene namesi
Name:TF1
OrganismiBacillus phage SP01 (Bacteriophage SP01)
Taxonomic identifieri10685 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeSpounavirinaeSpounalikevirus
Virus hostiBacillus subtilis [TaxID: 1423]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 9999Transcription factor 1PRO_0000105082Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

1
99
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 1410Combined sources
Helixi21 – 3818Combined sources
Turni45 – 473Combined sources
Beta strandi49 – 535Combined sources
Beta strandi63 – 675Combined sources
Beta strandi76 – 805Combined sources
Helixi83 – 9210Combined sources
Helixi94 – 985Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EXENMR-A/B1-99[»]
1WTUNMR-A/B1-99[»]
ProteinModelPortaliP04445.
SMRiP04445. Positions 1-99.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04445.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni52 – 7726May be involved in preference for HM-URA DNAAdd
BLAST
Regioni90 – 9910May be involved in preference for HM-URA DNA

Sequence similaritiesi

Phylogenomic databases

KOiK03530.

Family and domain databases

Gene3Di4.10.520.10. 1 hit.
InterProiIPR000119. Hist_DNA-bd.
IPR020816. Histone-like_DNA-bd_CS.
IPR010992. IHF-like_DNA-bd_dom.
[Graphical view]
PfamiPF00216. Bac_DNA_binding. 1 hit.
[Graphical view]
PRINTSiPR01727. DNABINDINGHU.
SMARTiSM00411. BHL. 1 hit.
[Graphical view]
SUPFAMiSSF47729. SSF47729. 1 hit.
PROSITEiPS00045. HISTONE_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04445-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKTELIKAI AQDTELTQVS VSKMLASFEK ITTETVAKGD KVQLTGFLNI
60 70 80 90
KPVARQARKG FNPQTQEALE IAPSVGVSVK PGESLKKAAE GLKYEDFAK
Length:99
Mass (Da):10,737
Last modified:August 13, 1987 - v1
Checksum:i3F8CD237A46EFC37
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02381 Genomic DNA. Translation: AAA32599.1.
PIRiA02693. DNBP11.
RefSeqiYP_002300416.1. NC_011421.1.

Genome annotation databases

GeneIDi7009134.
KEGGivg:7009134.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02381 Genomic DNA. Translation: AAA32599.1.
PIRiA02693. DNBP11.
RefSeqiYP_002300416.1. NC_011421.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EXENMR-A/B1-99[»]
1WTUNMR-A/B1-99[»]
ProteinModelPortaliP04445.
SMRiP04445. Positions 1-99.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi7009134.
KEGGivg:7009134.

Phylogenomic databases

KOiK03530.

Miscellaneous databases

EvolutionaryTraceiP04445.

Family and domain databases

Gene3Di4.10.520.10. 1 hit.
InterProiIPR000119. Hist_DNA-bd.
IPR020816. Histone-like_DNA-bd_CS.
IPR010992. IHF-like_DNA-bd_dom.
[Graphical view]
PfamiPF00216. Bac_DNA_binding. 1 hit.
[Graphical view]
PRINTSiPR01727. DNABINDINGHU.
SMARTiSM00411. BHL. 1 hit.
[Graphical view]
SUPFAMiSSF47729. SSF47729. 1 hit.
PROSITEiPS00045. HISTONE_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence of the bacteriophage SP01 gene coding for transcription factor 1, a viral homologue of the bacterial type II DNA-binding proteins."
    Greene J.R., Brennan S.M., Andrew D.J., Thompson C.C., Richards S.H., Heinrikson R.L., Geiduschek E.P.
    Proc. Natl. Acad. Sci. U.S.A. 81:7031-7035(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
  2. "Effects of mutations at amino acid 61 in the arm of TF1 on its DNA-binding properties."
    Sayre M.H., Geiduschek E.P.
    J. Mol. Biol. 216:819-833(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS.
  3. "A 1H-NMR study of the transcription factor 1 from Bacillus subtilis phage SPO1 by selective 2H-labeling. Complete assignment and structural analysis of the aromatic resonances for a 22-kDa homodimer."
    Reisman J.M., Hsu V.L., Jariel-Encontre I., Lecou C., Sayre M.H., Kearns D.R., Parello J.
    Eur. J. Biochem. 213:865-873(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.
  4. "Structure of the Bacillus subtilis phage SPO1-encoded type II DNA-binding protein TF1 in solution."
    Jia X., Grove A., Ivancic M., Hsu V.L., Geiduschek E.P., Kearns D.R.
    J. Mol. Biol. 263:259-268(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.

Entry informationi

Entry nameiTF1_BPSP1
AccessioniPrimary (citable) accession number: P04445
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: October 14, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.