Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

H-2 class II histocompatibility antigen gamma chain

Gene

Cd74

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to compartments where peptide loading of class II takes place.

GO - Molecular functioni

GO - Biological processi

  • activation of MAPK activity Source: Ensembl
  • antigen processing and presentation Source: MGI
  • antigen processing and presentation of exogenous peptide antigen via MHC class II Source: MGI
  • cell proliferation Source: UniProtKB
  • chaperone mediated protein folding requiring cofactor Source: MGI
  • defense response Source: MGI
  • immunoglobulin mediated immune response Source: MGI
  • intracellular protein transport Source: MGI
  • macrophage migration inhibitory factor signaling pathway Source: BHF-UCL
  • negative regulation of apoptotic process Source: UniProtKB
  • negative regulation of DNA damage response, signal transduction by p53 class mediator Source: BHF-UCL
  • negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: BHF-UCL
  • negative regulation of mature B cell apoptotic process Source: BHF-UCL
  • negative regulation of peptide secretion Source: MGI
  • negative regulation of T cell differentiation Source: MGI
  • negative thymic T cell selection Source: MGI
  • positive regulation of adaptive immune response Source: BHF-UCL
  • positive regulation of B cell proliferation Source: MGI
  • positive regulation of chemokine (C-X-C motif) ligand 2 production Source: BHF-UCL
  • positive regulation of cytokine-mediated signaling pathway Source: BHF-UCL
  • positive regulation of dendritic cell antigen processing and presentation Source: BHF-UCL
  • positive regulation of ERK1 and ERK2 cascade Source: BHF-UCL
  • positive regulation of fibroblast proliferation Source: MGI
  • positive regulation of innate immune response Source: BHF-UCL
  • positive regulation of macrophage cytokine production Source: BHF-UCL
  • positive regulation of neutrophil chemotaxis Source: BHF-UCL
  • positive regulation of peptidyl-tyrosine phosphorylation Source: BHF-UCL
  • positive regulation of T cell differentiation Source: MGI
  • positive regulation of type 2 immune response Source: BHF-UCL
  • positive thymic T cell selection Source: MGI
  • prostaglandin biosynthetic process Source: UniProtKB
  • protein complex assembly Source: MGI
  • signal transduction Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Adaptive immunity, Immunity

Enzyme and pathway databases

ReactomeiR-MMU-202733. Cell surface interactions at the vascular wall.
R-MMU-2132295. MHC class II antigen presentation.

Protein family/group databases

MEROPSiI31.002.

Names & Taxonomyi

Protein namesi
Recommended name:
H-2 class II histocompatibility antigen gamma chain
Alternative name(s):
Ia antigen-associated invariant chain
Short name:
Ii
MHC class II-associated invariant chain
CD_antigen: CD74
Gene namesi
Name:Cd74
Synonyms:Ii
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:96534. Cd74.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 29CytoplasmicSequence analysisAdd BLAST29
Transmembranei30 – 55Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST26
Topological domaini56 – 279ExtracellularSequence analysisAdd BLAST224

GO - Cellular componenti

  • cell surface Source: MGI
  • endoplasmic reticulum Source: MGI
  • external side of plasma membrane Source: MGI
  • extracellular exosome Source: MGI
  • Golgi apparatus Source: MGI
  • integral component of membrane Source: MGI
  • integral component of plasma membrane Source: MGI
  • late endosome Source: MGI
  • lysosome Source: MGI
  • macrophage migration inhibitory factor receptor complex Source: BHF-UCL
  • membrane Source: MGI
  • MHC class II protein complex Source: Ensembl
  • multivesicular body Source: MGI
  • NOS2-CD74 complex Source: BHF-UCL
  • plasma membrane Source: MGI
  • vacuole Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi265S → A: No addition of glycosaminoglycan; no affect the synthesis of protein. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000679551 – 279H-2 class II histocompatibility antigen gamma chainAdd BLAST279

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9PhosphoserineCombined sources1
Glycosylationi113N-linked (GlcNAc...)Sequence analysis1
Glycosylationi119N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi196 ↔ 215PROSITE-ProRule annotation
Disulfide bondi226 ↔ 233PROSITE-ProRule annotation
Disulfide bondi235 ↔ 254PROSITE-ProRule annotation
Glycosylationi265O-linked (Xyl...) (chondroitin sulfate)1 Publication1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Proteoglycan

Proteomic databases

PaxDbiP04441.
PeptideAtlasiP04441.
PRIDEiP04441.

PTM databases

iPTMnetiP04441.
PhosphoSitePlusiP04441.
SwissPalmiP04441.

Expressioni

Gene expression databases

BgeeiENSMUSG00000024610.
ExpressionAtlasiP04441. baseline and differential.
GenevisibleiP04441. MM.

Interactioni

Subunit structurei

Nonamer composed of three alpha/beta/gamma heterotrimers.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiP04441. 1 interactor.
MINTiMINT-1488725.
STRINGi10090.ENSMUSP00000095171.

Structurei

3D structure databases

ProteinModelPortaliP04441.
SMRiP04441.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini193 – 254Thyroglobulin type-1PROSITE-ProRule annotationAdd BLAST62

Sequence similaritiesi

Contains 1 thyroglobulin type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IY5J. Eukaryota.
ENOG410Z4PR. LUCA.
GeneTreeiENSGT00390000008961.
HOGENOMiHOG000013145.
HOVERGENiHBG004444.
InParanoidiP04441.
KOiK06505.
OMAiGTEVPNT.
OrthoDBiEOG091G0FT7.
PhylomeDBiP04441.
TreeFamiTF317779.

Family and domain databases

Gene3Di1.10.870.10. 1 hit.
4.10.800.10. 1 hit.
InterProiIPR015386. MHC_II-assoc_invar/CLIP_MHC-bd.
IPR022339. MHC_II-assoc_invar_chain.
IPR011988. MHC_II-assoc_invariant_trimer.
IPR000716. Thyroglobulin_1.
[Graphical view]
PfamiPF09307. MHC2-interact. 1 hit.
PF08831. MHCassoc_trimer. 1 hit.
PF00086. Thyroglobulin_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001992. CD74_antigen. 1 hit.
PRINTSiPR01990. CD74ANTIGEN.
SMARTiSM00211. TY. 1 hit.
[Graphical view]
SUPFAMiSSF48305. SSF48305. 1 hit.
SSF57610. SSF57610. 1 hit.
PROSITEiPS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P04441-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDDQRDLISN HEQLPILGNR PREPERCSRG ALYTGVSVLV ALLLAGQATT
60 70 80 90 100
AYFLYQQQGR LDKLTITSQN LQLESLRMKL PKSAKPVSQM RMATPLLMRP
110 120 130 140 150
MSMDNMLLGP VKNVTKYGNM TQDHVMHLLT RSGPLEYPQL KGTFPENLKH
160 170 180 190 200
LKNSMDGVNW KIFESWMKQW LLFEMSKNSL EEKKPTEAPP KVLTKCQEEV
210 220 230 240 250
SHIPAVYPGA FRPKCDENGN YLPLQCHGST GYCWCVFPNG TEVPHTKSRG
260 270
RHNCSEPLDM EDLSSGLGVT RQELGQVTL
Length:279
Mass (Da):31,557
Last modified:November 1, 1991 - v3
Checksum:i300A85014F170792
GO
Isoform Short (identifier: P04441-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     192-255: Missing.

Show »
Length:215
Mass (Da):24,454
Checksum:i090928B6B0F4200C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti10N → M (PubMed:2492095).Curated1
Sequence conflicti113N → Q (PubMed:2492095).Curated1
Sequence conflicti229 – 231STG → RHC in CAB37297 (PubMed:3038530).Curated3

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005332192 – 255Missing in isoform Short. 1 PublicationAdd BLAST64

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05428 Genomic DNA. Translation: CAA29010.1.
X05429 Genomic DNA. Translation: CAA29012.2.
X05430 Genomic DNA. Translation: CAB37297.1.
X13414 Genomic DNA. No translation available.
BC003476 mRNA. Translation: AAH03476.1.
X07129 mRNA. Translation: CAA30141.1.
M35872 Genomic DNA. Translation: AAA37897.1.
X00496 mRNA. Translation: CAA25191.1.
CCDSiCCDS37835.1. [P04441-1]
CCDS50299.1. [P04441-2]
PIRiB27866. HLHMSG.
RefSeqiNP_001036070.1. NM_001042605.1. [P04441-1]
UniGeneiMm.439737.

Genome annotation databases

EnsembliENSMUST00000050487; ENSMUSP00000057836; ENSMUSG00000024610. [P04441-2]
ENSMUST00000097563; ENSMUSP00000095171; ENSMUSG00000024610. [P04441-1]
ENSMUST00000167610; ENSMUSP00000126688; ENSMUSG00000024610. [P04441-1]
GeneIDi16149.
KEGGimmu:16149.
UCSCiuc008faz.1. mouse. [P04441-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05428 Genomic DNA. Translation: CAA29010.1.
X05429 Genomic DNA. Translation: CAA29012.2.
X05430 Genomic DNA. Translation: CAB37297.1.
X13414 Genomic DNA. No translation available.
BC003476 mRNA. Translation: AAH03476.1.
X07129 mRNA. Translation: CAA30141.1.
M35872 Genomic DNA. Translation: AAA37897.1.
X00496 mRNA. Translation: CAA25191.1.
CCDSiCCDS37835.1. [P04441-1]
CCDS50299.1. [P04441-2]
PIRiB27866. HLHMSG.
RefSeqiNP_001036070.1. NM_001042605.1. [P04441-1]
UniGeneiMm.439737.

3D structure databases

ProteinModelPortaliP04441.
SMRiP04441.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP04441. 1 interactor.
MINTiMINT-1488725.
STRINGi10090.ENSMUSP00000095171.

Protein family/group databases

MEROPSiI31.002.

PTM databases

iPTMnetiP04441.
PhosphoSitePlusiP04441.
SwissPalmiP04441.

Proteomic databases

PaxDbiP04441.
PeptideAtlasiP04441.
PRIDEiP04441.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000050487; ENSMUSP00000057836; ENSMUSG00000024610. [P04441-2]
ENSMUST00000097563; ENSMUSP00000095171; ENSMUSG00000024610. [P04441-1]
ENSMUST00000167610; ENSMUSP00000126688; ENSMUSG00000024610. [P04441-1]
GeneIDi16149.
KEGGimmu:16149.
UCSCiuc008faz.1. mouse. [P04441-1]

Organism-specific databases

CTDi972.
MGIiMGI:96534. Cd74.

Phylogenomic databases

eggNOGiENOG410IY5J. Eukaryota.
ENOG410Z4PR. LUCA.
GeneTreeiENSGT00390000008961.
HOGENOMiHOG000013145.
HOVERGENiHBG004444.
InParanoidiP04441.
KOiK06505.
OMAiGTEVPNT.
OrthoDBiEOG091G0FT7.
PhylomeDBiP04441.
TreeFamiTF317779.

Enzyme and pathway databases

ReactomeiR-MMU-202733. Cell surface interactions at the vascular wall.
R-MMU-2132295. MHC class II antigen presentation.

Miscellaneous databases

ChiTaRSiCd74. mouse.
PROiP04441.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024610.
ExpressionAtlasiP04441. baseline and differential.
GenevisibleiP04441. MM.

Family and domain databases

Gene3Di1.10.870.10. 1 hit.
4.10.800.10. 1 hit.
InterProiIPR015386. MHC_II-assoc_invar/CLIP_MHC-bd.
IPR022339. MHC_II-assoc_invar_chain.
IPR011988. MHC_II-assoc_invariant_trimer.
IPR000716. Thyroglobulin_1.
[Graphical view]
PfamiPF09307. MHC2-interact. 1 hit.
PF08831. MHCassoc_trimer. 1 hit.
PF00086. Thyroglobulin_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001992. CD74_antigen. 1 hit.
PRINTSiPR01990. CD74ANTIGEN.
SMARTiSM00211. TY. 1 hit.
[Graphical view]
SUPFAMiSSF48305. SSF48305. 1 hit.
SSF57610. SSF57610. 1 hit.
PROSITEiPS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHG2A_MOUSE
AccessioniPrimary (citable) accession number: P04441
Secondary accession number(s): O19452
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: November 1, 1991
Last modified: November 30, 2016
This is version 167 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.