Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P04441

- HG2A_MOUSE

UniProt

P04441 - HG2A_MOUSE

Protein

H-2 class II histocompatibility antigen gamma chain

Gene

Cd74

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 146 (01 Oct 2014)
      Sequence version 3 (01 Nov 1991)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to compartments where peptide loading of class II takes place.

    GO - Molecular functioni

    1. beta-amyloid binding Source: Ensembl
    2. cytokine binding Source: UniProtKB
    3. cytokine receptor activity Source: BHF-UCL
    4. MHC class II protein binding Source: BHF-UCL
    5. nitric-oxide synthase binding Source: BHF-UCL
    6. protein binding Source: BHF-UCL

    GO - Biological processi

    1. activation of MAPK activity Source: Ensembl
    2. antigen processing and presentation Source: MGI
    3. antigen processing and presentation of exogenous peptide antigen via MHC class II Source: MGI
    4. cell proliferation Source: UniProtKB
    5. chaperone mediated protein folding requiring cofactor Source: MGI
    6. defense response Source: MGI
    7. immunoglobulin mediated immune response Source: MGI
    8. intracellular protein transport Source: MGI
    9. macrophage migration inhibitory factor signaling pathway Source: BHF-UCL
    10. negative regulation of apoptotic process Source: UniProtKB
    11. negative regulation of DNA damage response, signal transduction by p53 class mediator Source: BHF-UCL
    12. negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: BHF-UCL
    13. negative regulation of mature B cell apoptotic process Source: BHF-UCL
    14. negative regulation of peptide secretion Source: Ensembl
    15. negative regulation of T cell differentiation Source: MGI
    16. negative thymic T cell selection Source: MGI
    17. positive regulation of adaptive immune response Source: BHF-UCL
    18. positive regulation of B cell proliferation Source: Ensembl
    19. positive regulation of chemokine (C-X-C motif) ligand 2 production Source: BHF-UCL
    20. positive regulation of cytokine-mediated signaling pathway Source: BHF-UCL
    21. positive regulation of dendritic cell antigen processing and presentation Source: BHF-UCL
    22. positive regulation of ERK1 and ERK2 cascade Source: BHF-UCL
    23. positive regulation of fibroblast proliferation Source: Ensembl
    24. positive regulation of innate immune response Source: BHF-UCL
    25. positive regulation of macrophage cytokine production Source: BHF-UCL
    26. positive regulation of neutrophil chemotaxis Source: BHF-UCL
    27. positive regulation of peptidyl-tyrosine phosphorylation Source: BHF-UCL
    28. positive regulation of T cell differentiation Source: MGI
    29. positive regulation of type 2 immune response Source: BHF-UCL
    30. positive thymic T cell selection Source: MGI
    31. prostaglandin biosynthetic process Source: UniProtKB
    32. protein complex assembly Source: MGI

    Keywords - Molecular functioni

    Chaperone

    Keywords - Biological processi

    Adaptive immunity, Immunity

    Enzyme and pathway databases

    ReactomeiREACT_196550. MHC class II antigen presentation.

    Protein family/group databases

    MEROPSiI31.002.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    H-2 class II histocompatibility antigen gamma chain
    Alternative name(s):
    Ia antigen-associated invariant chain
    Short name:
    Ii
    MHC class II-associated invariant chain
    CD_antigen: CD74
    Gene namesi
    Name:Cd74
    Synonyms:Ii
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 18

    Organism-specific databases

    MGIiMGI:96534. Cd74.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: MGI
    2. external side of plasma membrane Source: MGI
    3. Golgi apparatus Source: MGI
    4. integral component of plasma membrane Source: MGI
    5. late endosome Source: MGI
    6. lysosome Source: MGI
    7. macrophage migration inhibitory factor receptor complex Source: BHF-UCL
    8. multivesicular body Source: MGI
    9. NOS2-CD74 complex Source: BHF-UCL
    10. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi265 – 2651S → A: No addition of glycosaminoglycan; no affect the synthesis of protein. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 279279H-2 class II histocompatibility antigen gamma chainPRO_0000067955Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei9 – 91Phosphoserine1 Publication
    Glycosylationi113 – 1131N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi119 – 1191N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi196 ↔ 215PROSITE-ProRule annotation
    Disulfide bondi226 ↔ 233PROSITE-ProRule annotation
    Disulfide bondi235 ↔ 254PROSITE-ProRule annotation
    Glycosylationi265 – 2651O-linked (Xyl...) (chondroitin sulfate)1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein, Proteoglycan

    Proteomic databases

    MaxQBiP04441.
    PaxDbiP04441.
    PRIDEiP04441.

    PTM databases

    PhosphoSiteiP04441.

    Expressioni

    Gene expression databases

    ArrayExpressiP04441.
    BgeeiP04441.
    GenevestigatoriP04441.

    Interactioni

    Subunit structurei

    Nonamer composed of three alpha/beta/gamma heterotrimers.By similarity

    Protein-protein interaction databases

    IntActiP04441. 1 interaction.
    MINTiMINT-1488725.
    STRINGi10090.ENSMUSP00000095171.

    Structurei

    3D structure databases

    ProteinModelPortaliP04441.
    SMRiP04441. Positions 117-191, 193-256.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2929CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini56 – 279224ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei30 – 5526Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini193 – 25462Thyroglobulin type-1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 thyroglobulin type-1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG248215.
    HOGENOMiHOG000013145.
    HOVERGENiHBG004444.
    InParanoidiP04441.
    KOiK06505.
    OMAiQCDENGN.
    OrthoDBiEOG793B8J.
    PhylomeDBiP04441.
    TreeFamiTF317779.

    Family and domain databases

    Gene3Di1.10.870.10. 1 hit.
    4.10.800.10. 1 hit.
    InterProiIPR015386. MHC_II-assoc_invar/CLIP_MHC-bd.
    IPR022339. MHC_II-assoc_invar_chain.
    IPR011988. MHC_II-assoc_invariant_trimer.
    IPR000716. Thyroglobulin_1.
    [Graphical view]
    PANTHERiPTHR14093. PTHR14093. 1 hit.
    PfamiPF09307. MHC2-interact. 1 hit.
    PF08831. MHCassoc_trimer. 1 hit.
    PF00086. Thyroglobulin_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001992. CD74_antigen. 1 hit.
    PRINTSiPR01990. CD74ANTIGEN.
    SMARTiSM00211. TY. 1 hit.
    [Graphical view]
    SUPFAMiSSF48305. SSF48305. 1 hit.
    SSF57610. SSF57610. 1 hit.
    PROSITEiPS00484. THYROGLOBULIN_1_1. 1 hit.
    PS51162. THYROGLOBULIN_1_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Long (identifier: P04441-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDDQRDLISN HEQLPILGNR PREPERCSRG ALYTGVSVLV ALLLAGQATT    50
    AYFLYQQQGR LDKLTITSQN LQLESLRMKL PKSAKPVSQM RMATPLLMRP 100
    MSMDNMLLGP VKNVTKYGNM TQDHVMHLLT RSGPLEYPQL KGTFPENLKH 150
    LKNSMDGVNW KIFESWMKQW LLFEMSKNSL EEKKPTEAPP KVLTKCQEEV 200
    SHIPAVYPGA FRPKCDENGN YLPLQCHGST GYCWCVFPNG TEVPHTKSRG 250
    RHNCSEPLDM EDLSSGLGVT RQELGQVTL 279
    Length:279
    Mass (Da):31,557
    Last modified:November 1, 1991 - v3
    Checksum:i300A85014F170792
    GO
    Isoform Short (identifier: P04441-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         192-255: Missing.

    Show »
    Length:215
    Mass (Da):24,454
    Checksum:i090928B6B0F4200C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti10 – 101N → M(PubMed:2492095)Curated
    Sequence conflicti113 – 1131N → Q(PubMed:2492095)Curated
    Sequence conflicti229 – 2313STG → RHC in CAB37297. (PubMed:3038530)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei192 – 25564Missing in isoform Short. 1 PublicationVSP_005332Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X05428 Genomic DNA. Translation: CAA29010.1.
    X05429 Genomic DNA. Translation: CAA29012.2.
    X05430 Genomic DNA. Translation: CAB37297.1.
    X13414 Genomic DNA. No translation available.
    BC003476 mRNA. Translation: AAH03476.1.
    X07129 mRNA. Translation: CAA30141.1.
    M35872 Genomic DNA. Translation: AAA37897.1.
    X00496 mRNA. Translation: CAA25191.1.
    CCDSiCCDS37835.1. [P04441-1]
    CCDS50299.1. [P04441-2]
    PIRiB27866. HLHMSG.
    RefSeqiNP_001036070.1. NM_001042605.1. [P04441-1]
    UniGeneiMm.439737.

    Genome annotation databases

    EnsembliENSMUST00000050487; ENSMUSP00000057836; ENSMUSG00000024610. [P04441-2]
    ENSMUST00000097563; ENSMUSP00000095171; ENSMUSG00000024610. [P04441-1]
    ENSMUST00000167610; ENSMUSP00000126688; ENSMUSG00000024610. [P04441-1]
    GeneIDi16149.
    KEGGimmu:16149.
    UCSCiuc008faz.1. mouse. [P04441-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X05428 Genomic DNA. Translation: CAA29010.1 .
    X05429 Genomic DNA. Translation: CAA29012.2 .
    X05430 Genomic DNA. Translation: CAB37297.1 .
    X13414 Genomic DNA. No translation available.
    BC003476 mRNA. Translation: AAH03476.1 .
    X07129 mRNA. Translation: CAA30141.1 .
    M35872 Genomic DNA. Translation: AAA37897.1 .
    X00496 mRNA. Translation: CAA25191.1 .
    CCDSi CCDS37835.1. [P04441-1 ]
    CCDS50299.1. [P04441-2 ]
    PIRi B27866. HLHMSG.
    RefSeqi NP_001036070.1. NM_001042605.1. [P04441-1 ]
    UniGenei Mm.439737.

    3D structure databases

    ProteinModelPortali P04441.
    SMRi P04441. Positions 117-191, 193-256.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P04441. 1 interaction.
    MINTi MINT-1488725.
    STRINGi 10090.ENSMUSP00000095171.

    Protein family/group databases

    MEROPSi I31.002.

    PTM databases

    PhosphoSitei P04441.

    Proteomic databases

    MaxQBi P04441.
    PaxDbi P04441.
    PRIDEi P04441.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000050487 ; ENSMUSP00000057836 ; ENSMUSG00000024610 . [P04441-2 ]
    ENSMUST00000097563 ; ENSMUSP00000095171 ; ENSMUSG00000024610 . [P04441-1 ]
    ENSMUST00000167610 ; ENSMUSP00000126688 ; ENSMUSG00000024610 . [P04441-1 ]
    GeneIDi 16149.
    KEGGi mmu:16149.
    UCSCi uc008faz.1. mouse. [P04441-1 ]

    Organism-specific databases

    CTDi 972.
    MGIi MGI:96534. Cd74.

    Phylogenomic databases

    eggNOGi NOG248215.
    HOGENOMi HOG000013145.
    HOVERGENi HBG004444.
    InParanoidi P04441.
    KOi K06505.
    OMAi QCDENGN.
    OrthoDBi EOG793B8J.
    PhylomeDBi P04441.
    TreeFami TF317779.

    Enzyme and pathway databases

    Reactomei REACT_196550. MHC class II antigen presentation.

    Miscellaneous databases

    ChiTaRSi CD74. mouse.
    NextBioi 288935.
    PROi P04441.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P04441.
    Bgeei P04441.
    Genevestigatori P04441.

    Family and domain databases

    Gene3Di 1.10.870.10. 1 hit.
    4.10.800.10. 1 hit.
    InterProi IPR015386. MHC_II-assoc_invar/CLIP_MHC-bd.
    IPR022339. MHC_II-assoc_invar_chain.
    IPR011988. MHC_II-assoc_invariant_trimer.
    IPR000716. Thyroglobulin_1.
    [Graphical view ]
    PANTHERi PTHR14093. PTHR14093. 1 hit.
    Pfami PF09307. MHC2-interact. 1 hit.
    PF08831. MHCassoc_trimer. 1 hit.
    PF00086. Thyroglobulin_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001992. CD74_antigen. 1 hit.
    PRINTSi PR01990. CD74ANTIGEN.
    SMARTi SM00211. TY. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48305. SSF48305. 1 hit.
    SSF57610. SSF57610. 1 hit.
    PROSITEi PS00484. THYROGLOBULIN_1_1. 1 hit.
    PS51162. THYROGLOBULIN_1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of the gene for the murine Ia antigen-associated invariant chains (Ii). An alternatively spliced exon encodes a cysteine-rich domain highly homologous to a repetitive sequence of thyroglobulin."
      Koch N., Lauer W., Habicht J., Dobberstein B.
      EMBO J. 6:1677-1683(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM LONG), ALTERNATIVE SPLICING.
      Strain: AKR.
    2. "Complete sequence of the murine invariant chain (Ii) gene."
      Zhu L., Jones P.P.
      Nucleic Acids Res. 17:447-448(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: AKR.
      Tissue: Liver.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
      Tissue: Mammary gland.
    4. "Nucleotide sequences of the murine Ia-associated invariant chain (Ii) and I-E (H-2S, Beta) chain expressible cDNA clones."
      Stone J., Perry R., Todd J.A., McDevitt H.O.
      Submitted (MAR-1988) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-58.
      Tissue: Spleen.
    5. "The IFN-gamma response of the murine invariant chain gene is mediated by a complex enhancer that includes several MHC class II consensus elements."
      Eades A.-M., Litfin M., Rahmsdorf H.J.
      J. Immunol. 144:4399-4409(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-25.
    6. "Structure of the murine Ia-associated invariant (Ii) chain as deduced from a cDNA clone."
      Singer P.A., Lauer W., Dembic Z., Mayer W.E., Lipp J., Koch N., Hammerling G., Klein J., Dobberstein B.
      EMBO J. 3:873-877(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 47-279 (ISOFORM SHORT).
    7. "Identification of the glycosaminoglycan-attachment site of mouse invariant-chain proteoglycan core protein by site-directed mutagenesis."
      Miller J., Hatch J.A., Simonis S., Cullen S.E.
      Proc. Natl. Acad. Sci. U.S.A. 85:1359-1363(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION AT SER-265, MUTAGENESIS OF SER-265.
    8. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiHG2A_MOUSE
    AccessioniPrimary (citable) accession number: P04441
    Secondary accession number(s): O19452
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: November 1, 1991
    Last modified: October 1, 2014
    This is version 146 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3