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P04441

- HG2A_MOUSE

UniProt

P04441 - HG2A_MOUSE

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Protein

H-2 class II histocompatibility antigen gamma chain

Gene

Cd74

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to compartments where peptide loading of class II takes place.

GO - Molecular functioni

  1. beta-amyloid binding Source: Ensembl
  2. cytokine binding Source: UniProtKB
  3. cytokine receptor activity Source: BHF-UCL
  4. MHC class II protein binding Source: BHF-UCL
  5. nitric-oxide synthase binding Source: BHF-UCL

GO - Biological processi

  1. activation of MAPK activity Source: Ensembl
  2. antigen processing and presentation Source: MGI
  3. antigen processing and presentation of exogenous peptide antigen via MHC class II Source: MGI
  4. cell proliferation Source: UniProtKB
  5. chaperone mediated protein folding requiring cofactor Source: MGI
  6. defense response Source: MGI
  7. immunoglobulin mediated immune response Source: MGI
  8. intracellular protein transport Source: MGI
  9. macrophage migration inhibitory factor signaling pathway Source: BHF-UCL
  10. negative regulation of apoptotic process Source: UniProtKB
  11. negative regulation of DNA damage response, signal transduction by p53 class mediator Source: BHF-UCL
  12. negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: BHF-UCL
  13. negative regulation of mature B cell apoptotic process Source: BHF-UCL
  14. negative regulation of peptide secretion Source: Ensembl
  15. negative regulation of T cell differentiation Source: MGI
  16. negative thymic T cell selection Source: MGI
  17. positive regulation of adaptive immune response Source: BHF-UCL
  18. positive regulation of B cell proliferation Source: Ensembl
  19. positive regulation of chemokine (C-X-C motif) ligand 2 production Source: BHF-UCL
  20. positive regulation of cytokine-mediated signaling pathway Source: BHF-UCL
  21. positive regulation of dendritic cell antigen processing and presentation Source: BHF-UCL
  22. positive regulation of ERK1 and ERK2 cascade Source: BHF-UCL
  23. positive regulation of fibroblast proliferation Source: Ensembl
  24. positive regulation of innate immune response Source: BHF-UCL
  25. positive regulation of macrophage cytokine production Source: BHF-UCL
  26. positive regulation of neutrophil chemotaxis Source: BHF-UCL
  27. positive regulation of peptidyl-tyrosine phosphorylation Source: BHF-UCL
  28. positive regulation of T cell differentiation Source: MGI
  29. positive regulation of type 2 immune response Source: BHF-UCL
  30. positive thymic T cell selection Source: MGI
  31. prostaglandin biosynthetic process Source: UniProtKB
  32. protein complex assembly Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Adaptive immunity, Immunity

Enzyme and pathway databases

ReactomeiREACT_196550. MHC class II antigen presentation.

Protein family/group databases

MEROPSiI31.002.

Names & Taxonomyi

Protein namesi
Recommended name:
H-2 class II histocompatibility antigen gamma chain
Alternative name(s):
Ia antigen-associated invariant chain
Short name:
Ii
MHC class II-associated invariant chain
CD_antigen: CD74
Gene namesi
Name:Cd74
Synonyms:Ii
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 18

Organism-specific databases

MGIiMGI:96534. Cd74.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: MGI
  2. external side of plasma membrane Source: MGI
  3. extracellular vesicular exosome Source: Ensembl
  4. Golgi apparatus Source: MGI
  5. integral component of plasma membrane Source: MGI
  6. late endosome Source: MGI
  7. lysosome Source: MGI
  8. macrophage migration inhibitory factor receptor complex Source: BHF-UCL
  9. multivesicular body Source: MGI
  10. NOS2-CD74 complex Source: BHF-UCL
  11. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi265 – 2651S → A: No addition of glycosaminoglycan; no affect the synthesis of protein. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 279279H-2 class II histocompatibility antigen gamma chainPRO_0000067955Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei9 – 91Phosphoserine1 Publication
Glycosylationi113 – 1131N-linked (GlcNAc...)Sequence Analysis
Glycosylationi119 – 1191N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi196 ↔ 215PROSITE-ProRule annotation
Disulfide bondi226 ↔ 233PROSITE-ProRule annotation
Disulfide bondi235 ↔ 254PROSITE-ProRule annotation
Glycosylationi265 – 2651O-linked (Xyl...) (chondroitin sulfate)1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Proteoglycan

Proteomic databases

MaxQBiP04441.
PaxDbiP04441.
PRIDEiP04441.

PTM databases

PhosphoSiteiP04441.

Expressioni

Gene expression databases

BgeeiP04441.
ExpressionAtlasiP04441. baseline and differential.
GenevestigatoriP04441.

Interactioni

Subunit structurei

Nonamer composed of three alpha/beta/gamma heterotrimers.By similarity

Protein-protein interaction databases

IntActiP04441. 1 interaction.
MINTiMINT-1488725.
STRINGi10090.ENSMUSP00000095171.

Structurei

3D structure databases

ProteinModelPortaliP04441.
SMRiP04441. Positions 117-191, 193-256.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2929CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini56 – 279224ExtracellularSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei30 – 5526Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini193 – 25462Thyroglobulin type-1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 thyroglobulin type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG248215.
HOGENOMiHOG000013145.
HOVERGENiHBG004444.
InParanoidiP04441.
KOiK06505.
OMAiQCDENGN.
OrthoDBiEOG793B8J.
PhylomeDBiP04441.
TreeFamiTF317779.

Family and domain databases

Gene3Di1.10.870.10. 1 hit.
4.10.800.10. 1 hit.
InterProiIPR015386. MHC_II-assoc_invar/CLIP_MHC-bd.
IPR022339. MHC_II-assoc_invar_chain.
IPR011988. MHC_II-assoc_invariant_trimer.
IPR000716. Thyroglobulin_1.
[Graphical view]
PANTHERiPTHR14093. PTHR14093. 1 hit.
PfamiPF09307. MHC2-interact. 1 hit.
PF08831. MHCassoc_trimer. 1 hit.
PF00086. Thyroglobulin_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001992. CD74_antigen. 1 hit.
PRINTSiPR01990. CD74ANTIGEN.
SMARTiSM00211. TY. 1 hit.
[Graphical view]
SUPFAMiSSF48305. SSF48305. 1 hit.
SSF57610. SSF57610. 1 hit.
PROSITEiPS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Long (identifier: P04441-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDDQRDLISN HEQLPILGNR PREPERCSRG ALYTGVSVLV ALLLAGQATT
60 70 80 90 100
AYFLYQQQGR LDKLTITSQN LQLESLRMKL PKSAKPVSQM RMATPLLMRP
110 120 130 140 150
MSMDNMLLGP VKNVTKYGNM TQDHVMHLLT RSGPLEYPQL KGTFPENLKH
160 170 180 190 200
LKNSMDGVNW KIFESWMKQW LLFEMSKNSL EEKKPTEAPP KVLTKCQEEV
210 220 230 240 250
SHIPAVYPGA FRPKCDENGN YLPLQCHGST GYCWCVFPNG TEVPHTKSRG
260 270
RHNCSEPLDM EDLSSGLGVT RQELGQVTL
Length:279
Mass (Da):31,557
Last modified:November 1, 1991 - v3
Checksum:i300A85014F170792
GO
Isoform Short (identifier: P04441-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     192-255: Missing.

Show »
Length:215
Mass (Da):24,454
Checksum:i090928B6B0F4200C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti10 – 101N → M(PubMed:2492095)Curated
Sequence conflicti113 – 1131N → Q(PubMed:2492095)Curated
Sequence conflicti229 – 2313STG → RHC in CAB37297. (PubMed:3038530)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei192 – 25564Missing in isoform Short. 1 PublicationVSP_005332Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X05428 Genomic DNA. Translation: CAA29010.1.
X05429 Genomic DNA. Translation: CAA29012.2.
X05430 Genomic DNA. Translation: CAB37297.1.
X13414 Genomic DNA. No translation available.
BC003476 mRNA. Translation: AAH03476.1.
X07129 mRNA. Translation: CAA30141.1.
M35872 Genomic DNA. Translation: AAA37897.1.
X00496 mRNA. Translation: CAA25191.1.
CCDSiCCDS37835.1. [P04441-1]
CCDS50299.1. [P04441-2]
PIRiB27866. HLHMSG.
RefSeqiNP_001036070.1. NM_001042605.1. [P04441-1]
UniGeneiMm.439737.

Genome annotation databases

EnsembliENSMUST00000050487; ENSMUSP00000057836; ENSMUSG00000024610. [P04441-2]
ENSMUST00000097563; ENSMUSP00000095171; ENSMUSG00000024610. [P04441-1]
ENSMUST00000167610; ENSMUSP00000126688; ENSMUSG00000024610. [P04441-1]
GeneIDi16149.
KEGGimmu:16149.
UCSCiuc008faz.1. mouse. [P04441-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X05428 Genomic DNA. Translation: CAA29010.1 .
X05429 Genomic DNA. Translation: CAA29012.2 .
X05430 Genomic DNA. Translation: CAB37297.1 .
X13414 Genomic DNA. No translation available.
BC003476 mRNA. Translation: AAH03476.1 .
X07129 mRNA. Translation: CAA30141.1 .
M35872 Genomic DNA. Translation: AAA37897.1 .
X00496 mRNA. Translation: CAA25191.1 .
CCDSi CCDS37835.1. [P04441-1 ]
CCDS50299.1. [P04441-2 ]
PIRi B27866. HLHMSG.
RefSeqi NP_001036070.1. NM_001042605.1. [P04441-1 ]
UniGenei Mm.439737.

3D structure databases

ProteinModelPortali P04441.
SMRi P04441. Positions 117-191, 193-256.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P04441. 1 interaction.
MINTi MINT-1488725.
STRINGi 10090.ENSMUSP00000095171.

Protein family/group databases

MEROPSi I31.002.

PTM databases

PhosphoSitei P04441.

Proteomic databases

MaxQBi P04441.
PaxDbi P04441.
PRIDEi P04441.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000050487 ; ENSMUSP00000057836 ; ENSMUSG00000024610 . [P04441-2 ]
ENSMUST00000097563 ; ENSMUSP00000095171 ; ENSMUSG00000024610 . [P04441-1 ]
ENSMUST00000167610 ; ENSMUSP00000126688 ; ENSMUSG00000024610 . [P04441-1 ]
GeneIDi 16149.
KEGGi mmu:16149.
UCSCi uc008faz.1. mouse. [P04441-1 ]

Organism-specific databases

CTDi 972.
MGIi MGI:96534. Cd74.

Phylogenomic databases

eggNOGi NOG248215.
HOGENOMi HOG000013145.
HOVERGENi HBG004444.
InParanoidi P04441.
KOi K06505.
OMAi QCDENGN.
OrthoDBi EOG793B8J.
PhylomeDBi P04441.
TreeFami TF317779.

Enzyme and pathway databases

Reactomei REACT_196550. MHC class II antigen presentation.

Miscellaneous databases

ChiTaRSi CD74. mouse.
NextBioi 288935.
PROi P04441.
SOURCEi Search...

Gene expression databases

Bgeei P04441.
ExpressionAtlasi P04441. baseline and differential.
Genevestigatori P04441.

Family and domain databases

Gene3Di 1.10.870.10. 1 hit.
4.10.800.10. 1 hit.
InterProi IPR015386. MHC_II-assoc_invar/CLIP_MHC-bd.
IPR022339. MHC_II-assoc_invar_chain.
IPR011988. MHC_II-assoc_invariant_trimer.
IPR000716. Thyroglobulin_1.
[Graphical view ]
PANTHERi PTHR14093. PTHR14093. 1 hit.
Pfami PF09307. MHC2-interact. 1 hit.
PF08831. MHCassoc_trimer. 1 hit.
PF00086. Thyroglobulin_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF001992. CD74_antigen. 1 hit.
PRINTSi PR01990. CD74ANTIGEN.
SMARTi SM00211. TY. 1 hit.
[Graphical view ]
SUPFAMi SSF48305. SSF48305. 1 hit.
SSF57610. SSF57610. 1 hit.
PROSITEi PS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structure of the gene for the murine Ia antigen-associated invariant chains (Ii). An alternatively spliced exon encodes a cysteine-rich domain highly homologous to a repetitive sequence of thyroglobulin."
    Koch N., Lauer W., Habicht J., Dobberstein B.
    EMBO J. 6:1677-1683(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM LONG), ALTERNATIVE SPLICING.
    Strain: AKR.
  2. "Complete sequence of the murine invariant chain (Ii) gene."
    Zhu L., Jones P.P.
    Nucleic Acids Res. 17:447-448(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: AKR.
    Tissue: Liver.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
    Tissue: Mammary gland.
  4. "Nucleotide sequences of the murine Ia-associated invariant chain (Ii) and I-E (H-2S, Beta) chain expressible cDNA clones."
    Stone J., Perry R., Todd J.A., McDevitt H.O.
    Submitted (MAR-1988) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-58.
    Tissue: Spleen.
  5. "The IFN-gamma response of the murine invariant chain gene is mediated by a complex enhancer that includes several MHC class II consensus elements."
    Eades A.-M., Litfin M., Rahmsdorf H.J.
    J. Immunol. 144:4399-4409(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-25.
  6. "Structure of the murine Ia-associated invariant (Ii) chain as deduced from a cDNA clone."
    Singer P.A., Lauer W., Dembic Z., Mayer W.E., Lipp J., Koch N., Hammerling G., Klein J., Dobberstein B.
    EMBO J. 3:873-877(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 47-279 (ISOFORM SHORT).
  7. "Identification of the glycosaminoglycan-attachment site of mouse invariant-chain proteoglycan core protein by site-directed mutagenesis."
    Miller J., Hatch J.A., Simonis S., Cullen S.E.
    Proc. Natl. Acad. Sci. U.S.A. 85:1359-1363(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION AT SER-265, MUTAGENESIS OF SER-265.
  8. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHG2A_MOUSE
AccessioniPrimary (citable) accession number: P04441
Secondary accession number(s): O19452
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: November 1, 1991
Last modified: October 29, 2014
This is version 147 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3