Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P04441 (HG2A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 141. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
H-2 class II histocompatibility antigen gamma chain
Alternative name(s):
Ia antigen-associated invariant chain
Short name=Ii
MHC class II-associated invariant chain
CD_antigen=CD74
Gene names
Name:Cd74
Synonyms:Ii
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length279 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to compartments where peptide loading of class II takes place.

Subunit structure

Nonamer composed of three alpha/beta/gamma heterotrimers By similarity.

Subcellular location

Membrane; Single-pass type II membrane protein Potential.

Sequence similarities

Contains 1 thyroglobulin type-1 domain.

Ontologies

Keywords
   Biological processAdaptive immunity
Immunity
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainSignal-anchor
Transmembrane
Transmembrane helix
   Molecular functionChaperone
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
Proteoglycan
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactivation of MAPK activity

Inferred from electronic annotation. Source: Ensembl

antigen processing and presentation

Inferred from direct assay PubMed 11754812PubMed 7985028. Source: MGI

antigen processing and presentation of exogenous peptide antigen via MHC class II

Inferred from mutant phenotype PubMed 7679955PubMed 8598040. Source: MGI

cell proliferation

Inferred from sequence or structural similarity. Source: UniProtKB

chaperone mediated protein folding requiring cofactor

Inferred from direct assay PubMed 7985028. Source: MGI

defense response

Inferred from mutant phenotype PubMed 8098731PubMed 8294875. Source: MGI

immunoglobulin mediated immune response

Inferred from mutant phenotype PubMed 7679955. Source: MGI

intracellular protein transport

Inferred from direct assay PubMed 11754812PubMed 15294975. Source: MGI

macrophage migration inhibitory factor signaling pathway

Inferred from direct assay PubMed 19413900. Source: BHF-UCL

negative regulation of DNA damage response, signal transduction by p53 class mediator

Inferred from mutant phenotype PubMed 17045821. Source: BHF-UCL

negative regulation of T cell differentiation

Inferred from mutant phenotype PubMed 7679955. Source: MGI

negative regulation of apoptotic process

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator

Inferred from mutant phenotype PubMed 17045821. Source: BHF-UCL

negative regulation of mature B cell apoptotic process

Inferred from mutant phenotype PubMed 18056708. Source: BHF-UCL

negative regulation of peptide secretion

Inferred from electronic annotation. Source: Ensembl

negative thymic T cell selection

Inferred from mutant phenotype PubMed 7679955. Source: MGI

positive regulation of B cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of ERK1 and ERK2 cascade

Inferred from mutant phenotype PubMed 12782713. Source: BHF-UCL

positive regulation of T cell differentiation

Inferred from mutant phenotype PubMed 7679955PubMed 8598040PubMed 9432982. Source: MGI

positive regulation of adaptive immune response

Non-traceable author statement PubMed 18056708. Source: BHF-UCL

positive regulation of chemokine (C-X-C motif) ligand 2 production

Inferred from direct assay PubMed 19413900. Source: BHF-UCL

positive regulation of cytokine-mediated signaling pathway

Inferred from mutant phenotype PubMed 12782713. Source: BHF-UCL

positive regulation of dendritic cell antigen processing and presentation

Inferred from direct assay PubMed 18003616. Source: BHF-UCL

positive regulation of fibroblast proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of innate immune response

Inferred by curator PubMed 19413900. Source: BHF-UCL

positive regulation of macrophage cytokine production

Inferred from direct assay PubMed 19413900. Source: BHF-UCL

positive regulation of neutrophil chemotaxis

Inferred from direct assay PubMed 19413900. Source: BHF-UCL

positive regulation of peptidyl-tyrosine phosphorylation

Inferred from mutant phenotype PubMed 17045821. Source: BHF-UCL

positive regulation of type 2 immune response

Inferred from mutant phenotype PubMed 11823488. Source: BHF-UCL

positive thymic T cell selection

Inferred from mutant phenotype PubMed 7679955PubMed 8598040PubMed 9432982. Source: MGI

prostaglandin biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

protein complex assembly

Inferred from direct assay PubMed 7985028. Source: MGI

   Cellular_componentGolgi apparatus

Inferred from direct assay PubMed 11854359. Source: MGI

NOS2-CD74 complex

Inferred from direct assay PubMed 18003616. Source: BHF-UCL

endoplasmic reticulum

Inferred from direct assay PubMed 11854359. Source: MGI

external side of plasma membrane

Inferred from direct assay PubMed 15322158PubMed 19723499PubMed 7679955. Source: MGI

integral component of plasma membrane

Traceable author statement PubMed 7679955. Source: MGI

late endosome

Inferred from direct assay PubMed 15294975. Source: MGI

lysosome

Inferred from direct assay PubMed 11854359PubMed 15294975. Source: MGI

macrophage migration inhibitory factor receptor complex

Inferred from direct assay PubMed 12782713PubMed 18056708. Source: BHF-UCL

multivesicular body

Inferred from direct assay PubMed 11854359. Source: MGI

plasma membrane

Inferred from direct assay PubMed 11854359PubMed 8294875. Source: MGI

   Molecular_functionMHC class II protein binding

Inferred from physical interaction PubMed 7985028. Source: BHF-UCL

beta-amyloid binding

Inferred from electronic annotation. Source: Ensembl

cytokine binding

Inferred from sequence or structural similarity. Source: UniProtKB

cytokine receptor activity

Inferred from mutant phenotype PubMed 12782713. Source: BHF-UCL

nitric-oxide synthase binding

Inferred from physical interaction PubMed 18003616. Source: BHF-UCL

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: P04441-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: P04441-2)

The sequence of this isoform differs from the canonical sequence as follows:
     192-255: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 279279H-2 class II histocompatibility antigen gamma chain
PRO_0000067955

Regions

Topological domain1 – 2929Cytoplasmic Potential
Transmembrane30 – 5526Helical; Signal-anchor for type II membrane protein; Potential
Topological domain56 – 279224Extracellular Potential
Domain193 – 25462Thyroglobulin type-1

Amino acid modifications

Modified residue91Phosphoserine Ref.8
Glycosylation1131N-linked (GlcNAc...) Potential
Glycosylation1191N-linked (GlcNAc...) Potential
Glycosylation2651O-linked (Xyl...) (chondroitin sulfate) Ref.7
Disulfide bond196 ↔ 215 By similarity
Disulfide bond226 ↔ 233 By similarity
Disulfide bond235 ↔ 254 By similarity

Natural variations

Alternative sequence192 – 25564Missing in isoform Short.
VSP_005332

Experimental info

Mutagenesis2651S → A: No addition of glycosaminoglycan; no affect the synthesis of protein. Ref.7
Sequence conflict101N → M Ref.2
Sequence conflict1131N → Q Ref.2
Sequence conflict229 – 2313STG → RHC in CAB37297. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified November 1, 1991. Version 3.
Checksum: 300A85014F170792

FASTA27931,557
        10         20         30         40         50         60 
MDDQRDLISN HEQLPILGNR PREPERCSRG ALYTGVSVLV ALLLAGQATT AYFLYQQQGR 

        70         80         90        100        110        120 
LDKLTITSQN LQLESLRMKL PKSAKPVSQM RMATPLLMRP MSMDNMLLGP VKNVTKYGNM 

       130        140        150        160        170        180 
TQDHVMHLLT RSGPLEYPQL KGTFPENLKH LKNSMDGVNW KIFESWMKQW LLFEMSKNSL 

       190        200        210        220        230        240 
EEKKPTEAPP KVLTKCQEEV SHIPAVYPGA FRPKCDENGN YLPLQCHGST GYCWCVFPNG 

       250        260        270 
TEVPHTKSRG RHNCSEPLDM EDLSSGLGVT RQELGQVTL 

« Hide

Isoform Short [UniParc].

Checksum: 090928B6B0F4200C
Show »

FASTA21524,454

References

« Hide 'large scale' references
[1]"Primary structure of the gene for the murine Ia antigen-associated invariant chains (Ii). An alternatively spliced exon encodes a cysteine-rich domain highly homologous to a repetitive sequence of thyroglobulin."
Koch N., Lauer W., Habicht J., Dobberstein B.
EMBO J. 6:1677-1683(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM LONG), ALTERNATIVE SPLICING.
Strain: AKR.
[2]"Complete sequence of the murine invariant chain (Ii) gene."
Zhu L., Jones P.P.
Nucleic Acids Res. 17:447-448(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: AKR.
Tissue: Liver.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
Tissue: Mammary gland.
[4]"Nucleotide sequences of the murine Ia-associated invariant chain (Ii) and I-E (H-2S, Beta) chain expressible cDNA clones."
Stone J., Perry R., Todd J.A., McDevitt H.O.
Submitted (MAR-1988) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-58.
Tissue: Spleen.
[5]"The IFN-gamma response of the murine invariant chain gene is mediated by a complex enhancer that includes several MHC class II consensus elements."
Eades A.-M., Litfin M., Rahmsdorf H.J.
J. Immunol. 144:4399-4409(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-25.
[6]"Structure of the murine Ia-associated invariant (Ii) chain as deduced from a cDNA clone."
Singer P.A., Lauer W., Dembic Z., Mayer W.E., Lipp J., Koch N., Hammerling G., Klein J., Dobberstein B.
EMBO J. 3:873-877(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 47-279 (ISOFORM SHORT).
[7]"Identification of the glycosaminoglycan-attachment site of mouse invariant-chain proteoglycan core protein by site-directed mutagenesis."
Miller J., Hatch J.A., Simonis S., Cullen S.E.
Proc. Natl. Acad. Sci. U.S.A. 85:1359-1363(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION AT SER-265, MUTAGENESIS OF SER-265.
[8]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X05428 Genomic DNA. Translation: CAA29010.1.
X05429 Genomic DNA. Translation: CAA29012.2.
X05430 Genomic DNA. Translation: CAB37297.1.
X13414 Genomic DNA. No translation available.
BC003476 mRNA. Translation: AAH03476.1.
X07129 mRNA. Translation: CAA30141.1.
M35872 Genomic DNA. Translation: AAA37897.1.
X00496 mRNA. Translation: CAA25191.1.
PIRHLHMSG. B27866.
RefSeqNP_001036070.1. NM_001042605.1.
UniGeneMm.439737.

3D structure databases

ProteinModelPortalP04441.
SMRP04441. Positions 117-191, 193-256.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP04441. 1 interaction.
MINTMINT-1488725.
STRING10090.ENSMUSP00000095171.

Protein family/group databases

MEROPSI31.002.

PTM databases

PhosphoSiteP04441.

Proteomic databases

PaxDbP04441.
PRIDEP04441.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000050487; ENSMUSP00000057836; ENSMUSG00000024610. [P04441-2]
ENSMUST00000097563; ENSMUSP00000095171; ENSMUSG00000024610. [P04441-1]
ENSMUST00000167610; ENSMUSP00000126688; ENSMUSG00000024610. [P04441-1]
GeneID16149.
KEGGmmu:16149.
UCSCuc008faz.1. mouse. [P04441-1]

Organism-specific databases

CTD972.
MGIMGI:96534. Cd74.

Phylogenomic databases

eggNOGNOG248215.
HOGENOMHOG000013145.
HOVERGENHBG004444.
InParanoidP04441.
KOK06505.
OMAQCDENGN.
OrthoDBEOG793B8J.
PhylomeDBP04441.
TreeFamTF317779.

Gene expression databases

ArrayExpressP04441.
BgeeP04441.
GenevestigatorP04441.

Family and domain databases

Gene3D1.10.870.10. 1 hit.
4.10.800.10. 1 hit.
InterProIPR015386. MHC_II-assoc_invar/CLIP_MHC-bd.
IPR022339. MHC_II-assoc_invar_chain.
IPR011988. MHC_II-assoc_invariant_trimer.
IPR000716. Thyroglobulin_1.
[Graphical view]
PANTHERPTHR14093. PTHR14093. 1 hit.
PfamPF09307. MHC2-interact. 1 hit.
PF08831. MHCassoc_trimer. 1 hit.
PF00086. Thyroglobulin_1. 1 hit.
[Graphical view]
PIRSFPIRSF001992. CD74_antigen. 1 hit.
PRINTSPR01990. CD74ANTIGEN.
SMARTSM00211. TY. 1 hit.
[Graphical view]
SUPFAMSSF48305. SSF48305. 1 hit.
SSF57610. SSF57610. 1 hit.
PROSITEPS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCD74. mouse.
NextBio288935.
PROP04441.
SOURCESearch...

Entry information

Entry nameHG2A_MOUSE
AccessionPrimary (citable) accession number: P04441
Secondary accession number(s): O19452
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: November 1, 1991
Last modified: April 16, 2014
This is version 141 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot