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P04439 (1A03_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 141. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
HLA class I histocompatibility antigen, A-3 alpha chain
Alternative name(s):
MHC class I antigen A*3
Gene names
Name:HLA-A
Synonyms:HLAA
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length365 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the presentation of foreign antigens to the immune system.

Subunit structure

Dimer of alpha chain and a beta chain (beta-2-microglobulin). Interacts with human herpesvirus 8 MIR1 protein By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Post-translational modification

Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system By similarity.

Polymorphism

The following alleles of A-3 are known: A*03:01 (A-3.1), A*03:02, A*03:04, A*03:05 and A*03:06. The sequence shown is that of A*03:01.

Sequence similarities

Belongs to the MHC class I family.

Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

Ontologies

Keywords
   Biological processHost-virus interaction
Immunity
   Cellular componentMembrane
MHC I
   Coding sequence diversityPolymorphism
   DomainSignal
Transmembrane
Transmembrane helix
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
Ubl conjugation
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processantigen processing and presentation of exogenous peptide antigen via MHC class I

Traceable author statement. Source: Reactome

antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent

Traceable author statement. Source: Reactome

antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent

Traceable author statement. Source: Reactome

antigen processing and presentation of peptide antigen via MHC class I

Traceable author statement. Source: Reactome

cytokine-mediated signaling pathway

Traceable author statement. Source: Reactome

immune response

Non-traceable author statement Ref.1. Source: UniProtKB

interferon-gamma-mediated signaling pathway

Traceable author statement. Source: Reactome

positive regulation of T cell mediated cytotoxicity

Inferred from electronic annotation. Source: InterPro

regulation of immune response

Traceable author statement. Source: Reactome

type I interferon signaling pathway

Traceable author statement. Source: Reactome

viral process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentER to Golgi transport vesicle membrane

Traceable author statement. Source: Reactome

Golgi apparatus

Inferred from sequence or structural similarity. Source: UniProt

Golgi membrane

Traceable author statement. Source: Reactome

MHC class I protein complex

Inferred from sequence or structural similarity. Source: UniProt

cell surface

Inferred from sequence or structural similarity. Source: UniProt

early endosome membrane

Traceable author statement. Source: Reactome

endoplasmic reticulum

Inferred from sequence or structural similarity. Source: UniProt

integral component of lumenal side of endoplasmic reticulum membrane

Traceable author statement. Source: Reactome

integral component of plasma membrane

Non-traceable author statement Ref.1. Source: UniProtKB

phagocytic vesicle membrane

Traceable author statement. Source: Reactome

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionTAP binding

Inferred from direct assay PubMed 8617941. Source: UniProt

beta-2-microglobulin binding

Inferred from sequence or structural similarity. Source: UniProt

peptide antigen binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

receptor binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424
Chain25 – 365341HLA class I histocompatibility antigen, A-3 alpha chain
PRO_0000018815

Regions

Topological domain25 – 308284Extracellular Potential
Transmembrane309 – 33224Helical; Potential
Topological domain333 – 36533Cytoplasmic Potential
Domain209 – 29587Ig-like C1-type
Region25 – 11490Alpha-1
Region115 – 20692Alpha-2
Region207 – 29892Alpha-3
Region299 – 30810Connecting peptide

Amino acid modifications

Modified residue3561Phosphoserine By similarity
Glycosylation1101N-linked (GlcNAc...) By similarity
Disulfide bond125 ↔ 188 Ref.12
Disulfide bond227 ↔ 283 Ref.12

Natural variations

Natural variant891R → G.
Corresponds to variant rs1059459 [ dbSNP | Ensembl ].
VAR_050310
Natural variant941Q → H.
Corresponds to variant rs1059463 [ dbSNP | Ensembl ].
VAR_050311
Natural variant1011D → N.
Corresponds to variant rs1136688 [ dbSNP | Ensembl ].
VAR_050312
Natural variant1211I → M.
Corresponds to variant rs1136695 [ dbSNP | Ensembl ].
VAR_050313
Natural variant1291S → P.
Corresponds to variant rs1136700 [ dbSNP | Ensembl ].
VAR_050314
Natural variant1311G → W.
Corresponds to variant rs1136702 [ dbSNP | Ensembl ].
VAR_050315
Natural variant1331F → L.
Corresponds to variant rs1059488 [ dbSNP | Ensembl ].
VAR_050316
Natural variant1511N → K.
Corresponds to variant rs1059509 [ dbSNP | Ensembl ].
VAR_050317
Natural variant1661I → T. Ref.13
Corresponds to variant rs1059516 [ dbSNP | Ensembl ].
VAR_050318
Natural variant1691R → H.
Corresponds to variant rs1059520 [ dbSNP | Ensembl ].
VAR_050319
Natural variant1761E → V in allele A*03:02.
Corresponds to variant rs9256983 [ dbSNP | Ensembl ].
VAR_004351
Natural variant1801L → Q in allele A*03:02.
VAR_004352
Natural variant1851D → E in allele A*03:05.
Corresponds to variant rs1059542 [ dbSNP | Ensembl ].
VAR_016604
Natural variant1861G → A in allele A*03:06.
VAR_033149
Natural variant1991G → R in allele A*03:04.
Corresponds to variant rs41559916 [ dbSNP | Ensembl ].
VAR_016605
Natural variant2051R → H.
Corresponds to variant rs17185861 [ dbSNP | Ensembl ].
VAR_050320

Experimental info

Sequence conflict3191G → A in AAB66582. Ref.8

Secondary structure

................................................... 365
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P04439 [UniParc].

Last modified August 22, 2003. Version 2.
Checksum: DEDFCEC4450E0580

FASTA36540,841
        10         20         30         40         50         60 
MAVMAPRTLL LLLSGALALT QTWAGSHSMR YFFTSVSRPG RGEPRFIAVG YVDDTQFVRF 

        70         80         90        100        110        120 
DSDAASQRME PRAPWIEQEG PEYWDQETRN VKAQSQTDRV DLGTLRGYYN QSEAGSHTIQ 

       130        140        150        160        170        180 
IMYGCDVGSD GRFLRGYRQD AYDGKDYIAL NEDLRSWTAA DMAAQITKRK WEAAHEAEQL 

       190        200        210        220        230        240 
RAYLDGTCVE WLRRYLENGK ETLQRTDPPK THMTHHPISD HEATLRCWAL GFYPAEITLT 

       250        260        270        280        290        300 
WQRDGEDQTQ DTELVETRPA GDGTFQKWAA VVVPSGEEQR YTCHVQHEGL PKPLTLRWEL 

       310        320        330        340        350        360 
SSQPTIPIVG IIAGLVLLGA VITGAVVAAV MWRRKSSDRK GGSYTQAASS DSAQGSDVSL 


TACKV 

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of a functional class I HLA gene, HLA-A3: implications for the evolution of HLA genes."
Strachan T., Sodoyer R., Damotte M., Jordan B.R.
EMBO J. 3:887-894(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ALLELE A*03:01).
[2]Ellexson M.E., Hildebrand W.H.
Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE (ALLELE A*03:01).
[3]"Homo sapiens 2,229,817bp genomic DNA of 6p21.3 HLA class I region."
Shiina S., Tamiya G., Oka A., Inoko H.
Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ALLELE A*03:01).
[4]"Molecular cloning and DNA sequence analysis of genes encoding cytotoxic T lymphocyte-defined HLA-A3 subtypes: the E1 subtype."
Cowan E.P., Jordan B.E., Coligan J.E.
J. Immunol. 135:2835-2841(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ALLELE A*03:02).
[5]"Complete coding sequence of HLA-A*0302."
Bettinotti M.P., Hadzikadic L., Adams S., Marincola F.M.
Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE (ALLELE A*03:02).
[6]"Full length sequence of an HLA-A*0301 intron 2 variant."
Mayor N.P.
Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[7]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"Complete cDNA coding sequence of a new HLA-A3 subtype (A*0304) with a new HLA polymorphism at exon 3."
Santos S., Balas A., Garcia-Sanchez F., Lillo R., Merino J.L., Vicario J.L.
Immunogenetics 49:360-361(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-341 (ALLELE A*03:04).
[9]"Novel human HLA-A alleles identified in potential bone marrow donors."
Becher M.P., Wu J., Williams T.
Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE OF 26-206 (ALLELE A*03:05).
[10]"Identification of a HLA-A*03 new variant."
Poli F., Frison S., Crespiatico L., Longhi E.
Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE OF 26-298 (ALLELE A*03:05).
Tissue: Blood.
[11]"Novel HLA-A locus alleles including A*01012, A*0306, A*0308, A*2616, A*2617, A*3009, A*3206, A*3403, A*3602 and A*6604."
Bradshaw D., Gans C.P., Jones P., Rizzuto G., Steiner N., Mitton W., Ng J., Koester R., Hartzman R.J., Hurley C.K.
Tissue Antigens 59:325-327(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 26-206 (ALLELE A*03:06).
[12]"Structure of HLA-A*0301 in complex with a peptide of proteolipid protein: insights into the role of HLA-A alleles in susceptibility to multiple sclerosis."
McMahon R.M., Friis L., Siebold C., Friese M.A., Fugger L., Jones E.Y.
Acta Crystallogr. D 67:447-454(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 25-298 (ALLELE A*03:01) IN COMPLEX WITH PLP1 ANTIGENIC PEPTIDE, DISULFIDE BONDS.
[13]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] THR-166, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X00492 Genomic DNA. Translation: CAA25162.1. Sequence problems.
U32184 mRNA. Translation: AAB63980.1.
BA000025 Genomic DNA. Translation: BAB63400.1.
AF217561 mRNA. Translation: AAF28734.1.
AF015930 mRNA. Translation: AAB66582.1.
AH008295 Genomic DNA. Translation: AAF03243.1.
AJ401085 Genomic DNA. Translation: CAC06086.1.
AJ401086 Genomic DNA. Translation: CAC06087.1.
AJ401087 Genomic DNA. Translation: CAC06088.1.
AJ748743 Genomic DNA. Translation: CAG38621.1.
AL671277 Genomic DNA. Translation: CAI18372.1.
AH009116 Genomic DNA. Translation: AAF29557.1.
CCDSCCDS34373.1.
PIRHLHUA3. A02192.
RefSeqNP_002107.3. NM_002116.7.
UniGeneHs.181244.
Hs.713441.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2XPGX-ray2.60A25-298[»]
3RL1X-ray2.00A25-298[»]
3RL2X-ray2.39A25-298[»]
ProteinModelPortalP04439.
SMRP04439. Positions 25-298.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109350. 33 interactions.
IntActP04439. 4 interactions.

Chemistry

ChEMBLCHEMBL2632.

Polymorphism databases

DMDM34223717.

Proteomic databases

MaxQBP04439.
PaxDbP04439.
PRIDEP04439.

Protocols and materials databases

DNASU3105.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000376809; ENSP00000366005; ENSG00000206503.
ENST00000396634; ENSP00000379873; ENSG00000206503.
GeneID3105.
KEGGhsa:3105.
UCSCuc003nok.3. human.

Organism-specific databases

CTD3105.
GeneCardsGC06P029949.
HGNCHGNC:4931. HLA-A.
MIM142800. gene.
neXtProtNX_P04439.
PharmGKBPA35055.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG42056.
HOVERGENHBG016709.
KOK06751.
OrthoDBEOG7JT6WQ.
PhylomeDBP04439.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.

Gene expression databases

ArrayExpressP04439.
BgeeP04439.
CleanExHS_HLA-A.
GenevestigatorP04439.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
3.30.500.10. 1 hit.
InterProIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011161. MHC_I-like_Ag-recog.
IPR011162. MHC_I/II-like_Ag-recog.
IPR027648. MHC_I_a.
IPR001039. MHC_I_a_a1/a2.
IPR010579. MHC_I_a_C.
[Graphical view]
PfamPF07654. C1-set. 1 hit.
PF00129. MHC_I. 1 hit.
PF06623. MHC_I_C. 1 hit.
[Graphical view]
PRINTSPR01638. MHCCLASSI.
SMARTSM00407. IGc1. 1 hit.
[Graphical view]
SUPFAMSSF54452. SSF54452. 1 hit.
PROSITEPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSHLA-A. human.
GeneWikiHLA-A.
GenomeRNAi3105.
NextBio12319.
PMAP-CutDBP04439.
PROP04439.
SOURCESearch...

Entry information

Entry name1A03_HUMAN
AccessionPrimary (citable) accession number: P04439
Secondary accession number(s): O19546 expand/collapse secondary AC list , O19756, Q65A82, Q9GJE6, Q9GJE7, Q9GJE8, Q9MYE6, Q9MYG4, Q9TPR8
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 22, 2003
Last modified: July 9, 2014
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM