P04426 (WNT1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proto-oncogene Wnt-1

Alternative name(s):
Proto-oncogene Int-1

Gene names

Name:	Wnt1
Synonyms:	Int-1, Wnt-1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters. Proeminent role in the induction of the mesencephalon and cerebellum. May play a crucial role in the morphogenesis of the neural tube and/or the early stages of CNS development. Ref.5 Ref.9 Ref.10
Subunit structure	Interacts with PORCN. Interacts with RSPO1, RSPO2 and RSPO3. Interacts with WLS. Ref.8 Ref.11 Ref.12
Subcellular location	Secreted › extracellular space › extracellular matrix.
Tissue specificity	Testis and mid-gestational embryos. In the testis, detected only in postmeiotic germ cells undergoing differentiation from round spermatids into mature spermatozoa. In the embryos, expression is restricted to the developing CNS in regions of the neural tube other than the telencephalon. Ref.7
Developmental stage	Accumulates throughout the neural plate at the anterior head folds of the 9 day embryo but only at its lateral tips in more posterior regions. Following neural tube closure, expression is restricted to specific regions of the dorsal wall of the brain ventricles and spinal cord, the ventral wall of the midbrain and the diencephalon, and the lateral walls of the neuroepithelium at the midbrain-hindbrain junction. Ref.6
Post-translational modification	Palmitoylation at Ser-224 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-93. Palmitoylation is necessary for proper trafficking to cell surface By similarity.
Involvement in disease	Many mouse mammary tumors induced by mouse mammary tumor virus (MMTV) contain a provirus integrated into a host cell region which has been named INT-1 (now Wnt1).
Sequence similarities	Belongs to the Wnt family.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Extracellular matrix Secreted
Disease	Proto-oncogene
Domain	Signal
Molecular function	Developmental protein
PTM	Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	BMP signaling pathway Inferred from sequence orthology PubMed 19850029. Source: MGI Spemann organizer formation Inferred from mutant phenotype PubMed 9192640. Source: BHF-UCL T cell differentiation in thymus Inferred from direct assay PubMed 11265645. Source: MGI Wnt receptor signaling pathway, calcium modulating pathway Inferred from Biological aspect of Ancestor. Source: RefGenome brain segmentation Inferred from Biological aspect of Ancestor. Source: RefGenome branching involved in ureteric bud morphogenesis Inferred from genetic interaction PubMed 16054034. Source: MGI canonical Wnt receptor signaling pathway Inferred from direct assay PubMed 12121999PubMed 15265686. Source: BHF-UCL canonical Wnt receptor signaling pathway involved in negative regulation of apoptotic process Inferred from electronic annotation. Source: Compara cellular response to peptide hormone stimulus Inferred from electronic annotation. Source: Compara central nervous system morphogenesis Inferred from mutant phenotype Ref.5. Source: BHF-UCL cerebellum formation Inferred from mutant phenotype Ref.5. Source: BHF-UCL diencephalon development Inferred from genetic interaction PubMed 18094027. Source: MGI embryonic axis specification Inferred from mutant phenotype PubMed 2534596. Source: BHF-UCL forebrain anterior/posterior pattern specification Inferred from genetic interaction PubMed 18094027. Source: MGI fourth ventricle development Inferred from Biological aspect of Ancestor. Source: RefGenome hematopoietic stem cell proliferation Inferred from direct assay PubMed 9160667. Source: MGI hepatocyte differentiation Inferred from Biological aspect of Ancestor. Source: RefGenome inner ear morphogenesis Inferred from genetic interaction PubMed 15961523. Source: MGI mesoderm morphogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome midbrain development Inferred from mutant phenotype Ref.5. Source: BHF-UCL midbrain-hindbrain boundary maturation during brain development Inferred from mutant phenotype PubMed 8555108. Source: MGI myoblast fusion Inferred from direct assay PubMed 15282335. Source: MGI negative regulation of DNA damage checkpoint Inferred from electronic annotation. Source: Compara negative regulation of cell aging Inferred from electronic annotation. Source: Compara negative regulation of cell-cell adhesion Inferred from electronic annotation. Source: Compara negative regulation of cell-substrate adhesion Inferred from electronic annotation. Source: Compara negative regulation of fat cell differentiation Inferred from mutant phenotype PubMed 10937998. Source: BHF-UCL negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process Inferred from direct assay PubMed 16601693. Source: MGI negative regulation of transcription from RNA polymerase II promoter Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of transforming growth factor beta receptor signaling pathway Inferred from direct assay PubMed 16601693. Source: MGI neuron fate determination Inferred from mutant phenotype PubMed 16339193. Source: MGI organ regeneration Inferred from electronic annotation. Source: Compara positive regulation of Notch signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of cell proliferation Inferred from direct assay PubMed 9160667. Source: MGI positive regulation of dermatome development Inferred from electronic annotation. Source: Compara positive regulation of fibroblast proliferation Inferred from electronic annotation. Source: Compara positive regulation of insulin-like growth factor receptor signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of lamellipodium assembly Inferred from electronic annotation. Source: Compara positive regulation of protein phosphorylation Inferred from direct assay PubMed 15143170. Source: MGI positive regulation of sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 15035989. Source: BHF-UCL positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 15574752PubMed 9769173. Source: MGI response to wounding Inferred from electronic annotation. Source: Compara signal transduction in response to DNA damage Inferred from electronic annotation. Source: Compara spinal cord association neuron differentiation Inferred from genetic interaction PubMed 11877374. Source: MGI ubiquitin-dependent SMAD protein catabolic process Inferred from direct assay PubMed 16601693. Source: MGI
Cellular_component	cell surface Inferred from direct assay PubMed 8710372. Source: MGI early endosome Inferred from Biological aspect of Ancestor. Source: RefGenome extracellular space Inferred from Biological aspect of Ancestor. Source: RefGenome late endosome Inferred from Biological aspect of Ancestor. Source: RefGenome membrane raft Inferred from Biological aspect of Ancestor. Source: RefGenome plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cytokine activity Inferred from direct assay PubMed 10557084. Source: BHF-UCL frizzled-2 binding Inferred from Biological aspect of Ancestor. Source: RefGenome transcription regulatory region DNA binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Kl	O35082	2	EBI-1570911,EBI-1570828

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 370	343	Proto-oncogene Wnt-1		PRO_0000041406
Amino acid modifications
	Lipidation	93	1	S-palmitoyl cysteine By similarity
	Lipidation	224	1	O-palmitoyl serine; by PORCN By similarity
	Glycosylation	29	1	N-linked (GlcNAc...) Potential
	Glycosylation	316	1	N-linked (GlcNAc...) Potential
	Glycosylation	346	1	N-linked (GlcNAc...) Potential
	Glycosylation	359	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P04426 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: 02EEB23109231A40	FASTA	370	41,086
	10 20 30 40 50 60 MGLWALLPSW VSTTLLLALT ALPAALAANS SGRWWGIVNI ASSTNLLTDS KSLQLVLEPS 70 80 90 100 110 120 LQLLSRKQRR LIRQNPGILH SVSGGLQSAV RECKWQFRNR RWNCPTAPGP HLFGKIVNRG 130 140 150 160 170 180 CRETAFIFAI TSAGVTHSVA RSCSEGSIES CTCDYRRRGP GGPDWHWGGC SDNIDFGRLF 190 200 210 220 230 240 GREFVDSGEK GRDLRFLMNL HNNEAGRTTV FSEMRQECKC HGMSGSCTVR TCWMRLPTLR 250 260 270 280 290 300 AVGDVLRDRF DGASRVLYGN RGSNRASRAE LLRLEPEDPA HKPPSPHDLV YFEKSPNFCT 310 320 330 340 350 360 YSGRLGTAGT AGRACNSSSP ALDGCELLCC GRGHRTRTQR VTERCNCTFH WCCHVSCRNC 370 THTRVLHECL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and nucleotide sequence of the putative mammary oncogene int-1; proviral insertions leave the protein-encoding domain intact." Ooyen A.V., Nusse R. Cell 39:233-240(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C3H.
[2]	"Nucleotide sequence and expression in vitro of cDNA derived from mRNA of int-1, a provirally activated mouse mammary oncogene." Fung Y.-K.T., Shackleford G.M., Brown A.M.C., Sanders G.S., Varmus H.E. Mol. Cell. Biol. 5:3337-3344(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Expression of multiple novel Wnt-1/int-1-related genes during fetal and adult mouse development." Gavin B.J., McMahon J.A., McMahon A.P. Genes Dev. 4:2319-2332(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II.
[5]	"Targeted disruption of the murine int-1 proto-oncogene resulting in severe abnormalities in midbrain and cerebellar development." Thomas K.R., Capecchi M.R. Nature 346:847-850(1990) [PubMed] [Europe PMC] [Abstract] Cited for: POSSIBLE FUNCTION.
[6]	"Expression of the proto-oncogene int-1 is restricted to specific neural cells in the developing mouse embryo." Wilkinson D.G., Bailes J.A., McMahon A.P. Cell 50:79-88(1987) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE. Strain: CBA/Ca. Tissue: Embryo.
[7]	"Expression of the proto-oncogene int-1 is restricted to postmeiotic male germ cells and the neural tube of mid-gestational embryos." Shackleford G.M., Varmus H.E. Cell 50:89-95(1987) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY. Strain: ICR.
[8]	"The evolutionarily conserved porcupine gene family is involved in the processing of the Wnt family." Tanaka K., Okabayashi H., Asashima M., Perrimon N., Kadowaki T. Eur. J. Biochem. 267:4300-4311(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PORCN.
[9]	"Mammalian Ryk is a Wnt coreceptor required for stimulation of neurite outgrowth." Lu W., Yamamoto V., Ortega B., Baltimore D. Cell 119:97-108(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[10]	"Ryk-mediated Wnt repulsion regulates posterior-directed growth of corticospinal tract." Liu Y., Shi J., Lu C.C., Wang Z.B., Lyuksyutova A.I., Song X.J., Zou Y. Nat. Neurosci. 8:1151-1159(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[11]	"Mouse cristin/R-spondin family proteins are novel ligands for the Frizzled 8 and LRP6 receptors and activate beta-catenin-dependent gene expression." Nam J.-S., Turcotte T.J., Smith P.F., Choi S., Yoon J.K. J. Biol. Chem. 281:13247-13257(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RSPO1; RSPO2 AND RSPO3.
[12]	"Reciprocal regulation of Wnt and Gpr177/mouse Wntless is required for embryonic axis formation." Fu J., Jiang M., Mirando A.J., Yu H.-M., Hsu W. Proc. Natl. Acad. Sci. U.S.A. 106:18598-18603(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH WLS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	K02593 Genomic DNA. Translation: AAA39321.1. M11943 mRNA. Translation: AAA39322.1. BC005449 mRNA. Translation: AAH05449.1.
IPI	IPI00115819.
PIR	TVMST1. A23447.
RefSeq	NP_067254.1. NM_021279.4.
UniGene	Mm.1123.
3D structure databases
ProteinModelPortal	P04426.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-39896N.
IntAct	P04426. 1 interaction.
STRING	10090.ENSMUSP00000023734.
PTM databases
PhosphoSite	P04426.
Proteomic databases
PRIDE	P04426.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000023734; ENSMUSP00000023734; ENSMUSG00000022997.
GeneID	22408.
KEGG	mmu:22408.
Organism-specific databases
CTD	7471.
MGI	MGI:98953. Wnt1.
Phylogenomic databases
eggNOG	NOG284879.
HOGENOM	HOG000039528.
HOVERGEN	HBG001595.
InParanoid	P04426.
KO	K03209.
OMA	AIKECKW.
OrthoDB	EOG4R23V2.
Gene expression databases
ArrayExpress	P04426.
Bgee	P04426.
CleanEx	MM_WNT1.
Genevestigator	P04426.
GermOnline	ENSMUSG00000022997. Mus musculus.
Family and domain databases
InterPro	IPR005817. Wnt. IPR009139. Wnt1. IPR018161. Wnt_CS. [Graphical view]
PANTHER	PTHR12027. PTHR12027. 1 hit. PTHR12027:SF15. PTHR12027:SF15. 1 hit.
Pfam	PF00110. wnt. 1 hit. [Graphical view]
PRINTS	PR01841. WNT1PROTEIN. PR01349. WNTPROTEIN.
SMART	SM00097. WNT1. 1 hit. [Graphical view]
PROSITE	PS00246. WNT1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	302801.
SOURCE	Search...

Entry information

Entry name

WNT1_MOUSE

Accession

Primary (citable) accession number: P04426

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	April 3, 2013
This is version 120 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program