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P04426 (WNT1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proto-oncogene Wnt-1
Alternative name(s):
Proto-oncogene Int-1
Gene names
Name:Wnt1
Synonyms:Int-1, Wnt-1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length370 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters. Proeminent role in the induction of the mesencephalon and cerebellum. May play a crucial role in the morphogenesis of the neural tube and/or the early stages of CNS development. Has a role in osteoblast function and bone development By similarity. Ref.5 Ref.9 Ref.10

Subunit structure

Interacts with PORCN. Interacts with RSPO1, RSPO2 and RSPO3. Interacts with WLS. Ref.8 Ref.11 Ref.12

Subcellular location

Secretedextracellular spaceextracellular matrix.

Tissue specificity

Testis and mid-gestational embryos. In the testis, detected only in postmeiotic germ cells undergoing differentiation from round spermatids into mature spermatozoa. In the embryos, expression is restricted to the developing CNS in regions of the neural tube other than the telencephalon. Expressed in osteoblast; expression levels increase with advancing osteoblast differentiation. Expressed in the brain, femur, spleen, and hematopoietic bone marrow. Ref.7 Ref.13 Ref.14

Developmental stage

Accumulates throughout the neural plate at the anterior head folds of the 9 day embryo but only at its lateral tips in more posterior regions. Following neural tube closure, expression is restricted to specific regions of the dorsal wall of the brain ventricles and spinal cord, the ventral wall of the midbrain and the diencephalon, and the lateral walls of the neuroepithelium at the midbrain-hindbrain junction. Ref.6

Post-translational modification

Palmitoylation at Ser-224 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-93. Palmitoylation is necessary for proper trafficking to cell surface By similarity.

Involvement in disease

Many mouse mammary tumors induced by mouse mammary tumor virus (MMTV) contain a provirus integrated into a host cell region which has been named INT-1 (now Wnt1).

Sequence similarities

Belongs to the Wnt family.

Ontologies

Keywords
   Biological processWnt signaling pathway
   Cellular componentExtracellular matrix
Secreted
   DiseaseProto-oncogene
   DomainSignal
   Molecular functionDevelopmental protein
   PTMGlycoprotein
Lipoprotein
Palmitate
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processBMP signaling pathway

Inferred from sequence orthology PubMed 19850029. Source: MGI

Spemann organizer formation

Inferred from mutant phenotype PubMed 9192640. Source: BHF-UCL

T cell differentiation in thymus

Inferred from direct assay PubMed 11265645. Source: MGI

Wnt signaling pathway

Inferred from direct assay PubMed 16207730. Source: MGI

bone development

Inferred from sequence or structural similarity. Source: UniProtKB

branching involved in ureteric bud morphogenesis

Inferred from genetic interaction PubMed 16054034. Source: MGI

canonical Wnt signaling pathway

Inferred from direct assay PubMed 12121999PubMed 15265686. Source: BHF-UCL

canonical Wnt signaling pathway involved in negative regulation of apoptotic process

Inferred from electronic annotation. Source: Ensembl

cell-cell signaling

Inferred from direct assay PubMed 10557084. Source: BHF-UCL

cellular response to peptide hormone stimulus

Inferred from electronic annotation. Source: Ensembl

central nervous system morphogenesis

Inferred from mutant phenotype Ref.5. Source: BHF-UCL

cerebellum formation

Inferred from mutant phenotype Ref.5. Source: BHF-UCL

diencephalon development

Inferred from genetic interaction PubMed 18094027. Source: MGI

embryonic axis specification

Inferred from mutant phenotype PubMed 2534596. Source: BHF-UCL

forebrain anterior/posterior pattern specification

Inferred from genetic interaction PubMed 18094027. Source: MGI

hematopoietic stem cell proliferation

Inferred from direct assay PubMed 9160667. Source: MGI

hepatocyte differentiation

Inferred from electronic annotation. Source: Ensembl

inner ear morphogenesis

Inferred from genetic interaction PubMed 15961523. Source: MGI

metencephalon development

Inferred from mutant phenotype PubMed 2205396. Source: MGI

midbrain development

Inferred from mutant phenotype Ref.5. Source: BHF-UCL

midbrain-hindbrain boundary development

Inferred from mutant phenotype PubMed 2205396. Source: MGI

midbrain-hindbrain boundary maturation during brain development

Inferred from mutant phenotype PubMed 8555108. Source: MGI

myoblast fusion

Inferred from direct assay PubMed 15282335. Source: MGI

myotube differentiation

Inferred from genetic interaction PubMed 15282335. Source: MGI

negative regulation of BMP signaling pathway

Inferred from electronic annotation. Source: Ensembl

negative regulation of cell aging

Inferred from electronic annotation. Source: Ensembl

negative regulation of cell differentiation

Inferred from mutant phenotype PubMed 9473323. Source: AgBase

negative regulation of cell-cell adhesion

Inferred from electronic annotation. Source: Ensembl

negative regulation of cell-substrate adhesion

Inferred from electronic annotation. Source: Ensembl

negative regulation of fat cell differentiation

Inferred from mutant phenotype PubMed 10937998. Source: BHF-UCL

negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process

Inferred from direct assay PubMed 16601693. Source: MGI

negative regulation of transforming growth factor beta receptor signaling pathway

Inferred from direct assay PubMed 16601693. Source: MGI

neuron fate commitment

Inferred from mutant phenotype PubMed 16339193. Source: MGI

neuron fate determination

Inferred from mutant phenotype PubMed 16339193. Source: MGI

organ morphogenesis

Traceable author statement PubMed 9889131. Source: MGI

organ regeneration

Inferred from electronic annotation. Source: Ensembl

positive regulation of Notch signaling pathway

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell proliferation

Inferred from direct assay PubMed 9160667. Source: MGI

positive regulation of dermatome development

Inferred from electronic annotation. Source: Ensembl

positive regulation of fibroblast proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of insulin-like growth factor receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

positive regulation of lamellipodium assembly

Inferred from electronic annotation. Source: Ensembl

positive regulation of protein phosphorylation

Inferred from direct assay PubMed 15143170. Source: MGI

positive regulation of sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 15035989. Source: BHF-UCL

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 15574752PubMed 9769173. Source: MGI

positive regulation of transcription, DNA-templated

Inferred from direct assay PubMed 12937339. Source: UniProtKB

response to wounding

Inferred from electronic annotation. Source: Ensembl

signal transduction

Traceable author statement PubMed 9889131. Source: MGI

signal transduction in response to DNA damage

Inferred from electronic annotation. Source: Ensembl

spinal cord association neuron differentiation

Inferred from genetic interaction PubMed 11877374. Source: MGI

ubiquitin-dependent SMAD protein catabolic process

Inferred from direct assay PubMed 16601693. Source: MGI

   Cellular_componentcell surface

Inferred from direct assay PubMed 8710372. Source: MGI

endoplasmic reticulum lumen

Traceable author statement. Source: Reactome

extracellular space

Inferred from Biological aspect of Ancestor. Source: RefGenome

proteinaceous extracellular matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncytokine activity

Inferred from direct assay PubMed 10557084. Source: BHF-UCL

frizzled binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

protein binding

Inferred from physical interaction Ref.12. Source: UniProtKB

protein domain specific binding

Inferred from physical interaction Ref.9. Source: UniProtKB

receptor binding

Traceable author statement PubMed 9889131. Source: MGI

transcription regulatory region DNA binding

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

KlO350822EBI-1570911,EBI-1570828
LRP6O755812EBI-1570911,EBI-910915From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 370343Proto-oncogene Wnt-1
PRO_0000041406

Amino acid modifications

Lipidation931S-palmitoyl cysteine By similarity
Lipidation2241O-palmitoyl serine; by PORCN By similarity
Glycosylation291N-linked (GlcNAc...) Potential
Glycosylation3161N-linked (GlcNAc...) Potential
Glycosylation3461N-linked (GlcNAc...) Potential
Glycosylation3591N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P04426 [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: 02EEB23109231A40

FASTA37041,086
        10         20         30         40         50         60 
MGLWALLPSW VSTTLLLALT ALPAALAANS SGRWWGIVNI ASSTNLLTDS KSLQLVLEPS 

        70         80         90        100        110        120 
LQLLSRKQRR LIRQNPGILH SVSGGLQSAV RECKWQFRNR RWNCPTAPGP HLFGKIVNRG 

       130        140        150        160        170        180 
CRETAFIFAI TSAGVTHSVA RSCSEGSIES CTCDYRRRGP GGPDWHWGGC SDNIDFGRLF 

       190        200        210        220        230        240 
GREFVDSGEK GRDLRFLMNL HNNEAGRTTV FSEMRQECKC HGMSGSCTVR TCWMRLPTLR 

       250        260        270        280        290        300 
AVGDVLRDRF DGASRVLYGN RGSNRASRAE LLRLEPEDPA HKPPSPHDLV YFEKSPNFCT 

       310        320        330        340        350        360 
YSGRLGTAGT AGRACNSSSP ALDGCELLCC GRGHRTRTQR VTERCNCTFH WCCHVSCRNC 

       370 
THTRVLHECL 

« Hide

References

« Hide 'large scale' references
[1]"Structure and nucleotide sequence of the putative mammary oncogene int-1; proviral insertions leave the protein-encoding domain intact."
Ooyen A.V., Nusse R.
Cell 39:233-240(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: C3H.
[2]"Nucleotide sequence and expression in vitro of cDNA derived from mRNA of int-1, a provirally activated mouse mammary oncogene."
Fung Y.-K.T., Shackleford G.M., Brown A.M.C., Sanders G.S., Varmus H.E.
Mol. Cell. Biol. 5:3337-3344(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Expression of multiple novel Wnt-1/int-1-related genes during fetal and adult mouse development."
Gavin B.J., McMahon J.A., McMahon A.P.
Genes Dev. 4:2319-2332(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Czech II.
[5]"Targeted disruption of the murine int-1 proto-oncogene resulting in severe abnormalities in midbrain and cerebellar development."
Thomas K.R., Capecchi M.R.
Nature 346:847-850(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: POSSIBLE FUNCTION.
[6]"Expression of the proto-oncogene int-1 is restricted to specific neural cells in the developing mouse embryo."
Wilkinson D.G., Bailes J.A., McMahon A.P.
Cell 50:79-88(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
Strain: CBA/Ca.
Tissue: Embryo.
[7]"Expression of the proto-oncogene int-1 is restricted to postmeiotic male germ cells and the neural tube of mid-gestational embryos."
Shackleford G.M., Varmus H.E.
Cell 50:89-95(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
Strain: ICR.
[8]"The evolutionarily conserved porcupine gene family is involved in the processing of the Wnt family."
Tanaka K., Okabayashi H., Asashima M., Perrimon N., Kadowaki T.
Eur. J. Biochem. 267:4300-4311(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PORCN.
[9]"Mammalian Ryk is a Wnt coreceptor required for stimulation of neurite outgrowth."
Lu W., Yamamoto V., Ortega B., Baltimore D.
Cell 119:97-108(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"Ryk-mediated Wnt repulsion regulates posterior-directed growth of corticospinal tract."
Liu Y., Shi J., Lu C.C., Wang Z.B., Lyuksyutova A.I., Song X.J., Zou Y.
Nat. Neurosci. 8:1151-1159(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[11]"Mouse cristin/R-spondin family proteins are novel ligands for the Frizzled 8 and LRP6 receptors and activate beta-catenin-dependent gene expression."
Nam J.-S., Turcotte T.J., Smith P.F., Choi S., Yoon J.K.
J. Biol. Chem. 281:13247-13257(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RSPO1; RSPO2 AND RSPO3.
[12]"Reciprocal regulation of Wnt and Gpr177/mouse Wntless is required for embryonic axis formation."
Fu J., Jiang M., Mirando A.J., Yu H.-M., Hsu W.
Proc. Natl. Acad. Sci. U.S.A. 106:18598-18603(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH WLS.
[13]"Mutations in WNT1 cause different forms of bone fragility."
Keupp K., Beleggia F., Kayserili H., Barnes A.M., Steiner M., Semler O., Fischer B., Yigit G., Janda C.Y., Becker J., Breer S., Altunoglu U., Gruenhagen J., Krawitz P., Hecht J., Schinke T., Makareeva E., Lausch E. expand/collapse author list , Cankaya T., Caparros-Martin J.A., Lapunzina P., Temtamy S., Aglan M., Zabel B., Eysel P., Koerber F., Leikin S., Garcia K.C., Netzer C., Schoenau E., Ruiz-Perez V.L., Mundlos S., Amling M., Kornak U., Marini J., Wollnik B.
Am. J. Hum. Genet. 92:565-574(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[14]"WNT1 mutations in early-onset osteoporosis and osteogenesis imperfecta."
Laine C.M., Joeng K.S., Campeau P.M., Kiviranta R., Tarkkonen K., Grover M., Lu J.T., Pekkinen M., Wessman M., Heino T.J., Nieminen-Pihala V., Aronen M., Laine T., Kroeger H., Cole W.G., Lehesjoki A.E., Nevarez L., Krakow D. expand/collapse author list , Curry C.J., Cohn D.H., Gibbs R.A., Lee B.H., Maekitie O.
N. Engl. J. Med. 368:1809-1816(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
K02593 Genomic DNA. Translation: AAA39321.1.
M11943 mRNA. Translation: AAA39322.1.
BC005449 mRNA. Translation: AAH05449.1.
CCDSCCDS27807.1.
PIRTVMST1. A23447.
RefSeqNP_067254.1. NM_021279.4.
XP_006520953.1. XM_006520890.1.
UniGeneMm.1123.

3D structure databases

ProteinModelPortalP04426.
SMRP04426. Positions 85-299.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204567. 9 interactions.
DIPDIP-39896N.
IntActP04426. 2 interactions.
STRING10090.ENSMUSP00000023734.

PTM databases

PhosphoSiteP04426.

Proteomic databases

PRIDEP04426.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000023734; ENSMUSP00000023734; ENSMUSG00000022997.
GeneID22408.
KEGGmmu:22408.
UCSCuc007xnx.1. mouse.

Organism-specific databases

CTD7471.
MGIMGI:98953. Wnt1.

Phylogenomic databases

eggNOGNOG284879.
HOGENOMHOG000039528.
HOVERGENHBG001595.
InParanoidP04426.
KOK03209.
OMAAIKECKW.
OrthoDBEOG7C8GJ8.
PhylomeDBP04426.
TreeFamTF105310.

Enzyme and pathway databases

ReactomeREACT_188257. Signal Transduction.

Gene expression databases

ArrayExpressP04426.
BgeeP04426.
CleanExMM_WNT1.
GenevestigatorP04426.

Family and domain databases

InterProIPR005817. Wnt.
IPR009139. Wnt1.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERPTHR12027. PTHR12027. 1 hit.
PfamPF00110. wnt. 1 hit.
[Graphical view]
PRINTSPR01841. WNT1PROTEIN.
PR01349. WNTPROTEIN.
SMARTSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEPS00246. WNT1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio302801.
PROP04426.
SOURCESearch...

Entry information

Entry nameWNT1_MOUSE
AccessionPrimary (citable) accession number: P04426
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: July 9, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot