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Protein

Proto-oncogene Wnt-1

Gene

Wnt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters. Proeminent role in the induction of the mesencephalon and cerebellum. May play a crucial role in the morphogenesis of the neural tube and/or the early stages of CNS development. Has a role in osteoblast function and bone development (By similarity).By similarity2 Publications

GO - Molecular functioni

  • cytokine activity Source: BHF-UCL
  • frizzled binding Source: GO_Central
  • protein domain specific binding Source: UniProtKB
  • receptor agonist activity Source: ParkinsonsUK-UCL
  • receptor binding Source: MGI
  • transcription regulatory region DNA binding Source: MGI

GO - Biological processi

  • animal organ morphogenesis Source: MGI
  • animal organ regeneration Source: Ensembl
  • astrocyte-dopaminergic neuron signaling Source: ParkinsonsUK-UCL
  • BMP signaling pathway Source: MGI
  • bone development Source: UniProtKB
  • branching involved in ureteric bud morphogenesis Source: MGI
  • canonical Wnt signaling pathway Source: BHF-UCL
  • canonical Wnt signaling pathway involved in midbrain dopaminergic neuron differentiation Source: ParkinsonsUK-UCL
  • canonical Wnt signaling pathway involved in negative regulation of apoptotic process Source: MGI
  • cell-cell signaling Source: BHF-UCL
  • cell proliferation in midbrain Source: ParkinsonsUK-UCL
  • cellular response to peptide hormone stimulus Source: Ensembl
  • central nervous system morphogenesis Source: BHF-UCL
  • cerebellum development Source: ParkinsonsUK-UCL
  • cerebellum formation Source: BHF-UCL
  • diencephalon development Source: MGI
  • dopaminergic neuron differentiation Source: CACAO
  • embryonic axis specification Source: BHF-UCL
  • embryonic brain development Source: ParkinsonsUK-UCL
  • forebrain anterior/posterior pattern specification Source: MGI
  • hematopoietic stem cell proliferation Source: MGI
  • hepatocyte differentiation Source: Ensembl
  • inner ear morphogenesis Source: MGI
  • metencephalon development Source: MGI
  • midbrain development Source: ParkinsonsUK-UCL
  • midbrain dopaminergic neuron differentiation Source: ParkinsonsUK-UCL
  • midbrain-hindbrain boundary development Source: MGI
  • midbrain-hindbrain boundary maturation during brain development Source: MGI
  • myoblast fusion Source: MGI
  • myotube differentiation Source: MGI
  • negative regulation of BMP signaling pathway Source: MGI
  • negative regulation of cell aging Source: MGI
  • negative regulation of cell-cell adhesion Source: MGI
  • negative regulation of cell differentiation Source: AgBase
  • negative regulation of cell-substrate adhesion Source: MGI
  • negative regulation of fat cell differentiation Source: BHF-UCL
  • negative regulation of oxidative stress-induced neuron death Source: ParkinsonsUK-UCL
  • negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: MGI
  • negative regulation of transforming growth factor beta receptor signaling pathway Source: MGI
  • neurogenesis Source: ParkinsonsUK-UCL
  • neuron fate commitment Source: MGI
  • neuron fate determination Source: MGI
  • positive regulation of canonical Wnt signaling pathway Source: MGI
  • positive regulation of cell proliferation Source: ParkinsonsUK-UCL
  • positive regulation of dermatome development Source: MGI
  • positive regulation of fibroblast proliferation Source: MGI
  • positive regulation of insulin-like growth factor receptor signaling pathway Source: MGI
  • positive regulation of lamellipodium assembly Source: MGI
  • positive regulation of Notch signaling pathway Source: MGI
  • positive regulation of protein phosphorylation Source: MGI
  • positive regulation of sequence-specific DNA binding transcription factor activity Source: BHF-UCL
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: ParkinsonsUK-UCL
  • regulation of transcription, DNA-templated Source: ParkinsonsUK-UCL
  • response to wounding Source: Ensembl
  • signal transduction Source: MGI
  • signal transduction in response to DNA damage Source: MGI
  • Spemann organizer formation Source: BHF-UCL
  • spinal cord association neuron differentiation Source: MGI
  • T cell differentiation in thymus Source: MGI
  • ubiquitin-dependent SMAD protein catabolic process Source: MGI
  • Wnt signaling pathway Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Wnt signaling pathway

Enzyme and pathway databases

ReactomeiR-MMU-3238698. WNT ligand biogenesis and trafficking.
R-MMU-381340. Transcriptional regulation of white adipocyte differentiation.
R-MMU-4086400. PCP/CE pathway.
R-MMU-442533. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.
R-MMU-4641262. Disassembly of the destruction complex and recruitment of AXIN to the membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Proto-oncogene Wnt-1
Alternative name(s):
Proto-oncogene Int-1
Gene namesi
Name:Wnt1
Synonyms:Int-1, Wnt-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:98953. Wnt1.

Subcellular locationi

GO - Cellular componenti

  • cell surface Source: MGI
  • cytoplasm Source: MGI
  • endoplasmic reticulum lumen Source: Reactome
  • extracellular region Source: ParkinsonsUK-UCL
  • extracellular space Source: GO_Central
  • proteinaceous extracellular matrix Source: UniProtKB-SubCell
  • Wnt signalosome Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000004140628 – 370Proto-oncogene Wnt-1Add BLAST343

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi29N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi93 ↔ 104By similarity
Disulfide bondi143 ↔ 151By similarity
Disulfide bondi153 ↔ 170By similarity
Disulfide bondi218 ↔ 232By similarity
Disulfide bondi220 ↔ 227By similarity
Lipidationi224O-palmitoleyl serine; by PORCNBy similarity1
Disulfide bondi315 ↔ 330By similarity
Glycosylationi316N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi345 ↔ 360By similarity
Glycosylationi346N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi347 ↔ 357By similarity
Disulfide bondi352 ↔ 353By similarity
Glycosylationi359N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Palmitoleylation is required for efficient binding to frizzled receptors. Palmitoleylation is necessary for proper trafficking to cell surface (By similarity). Depalmitoleylated by NOTUM, leading to inhibit Wnt signaling pathway (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein

Proteomic databases

PaxDbiP04426.
PRIDEiP04426.

PTM databases

PhosphoSitePlusiP04426.

Expressioni

Tissue specificityi

Testis and mid-gestational embryos. In the testis, detected only in postmeiotic germ cells undergoing differentiation from round spermatids into mature spermatozoa. In the embryos, expression is restricted to the developing CNS in regions of the neural tube other than the telencephalon. Expressed in osteoblast; expression levels increase with advancing osteoblast differentiation. Expressed in the brain, femur, spleen, and hematopoietic bone marrow.3 Publications

Developmental stagei

Accumulates throughout the neural plate at the anterior head folds of the 9 day embryo but only at its lateral tips in more posterior regions. Following neural tube closure, expression is restricted to specific regions of the dorsal wall of the brain ventricles and spinal cord, the ventral wall of the midbrain and the diencephalon, and the lateral walls of the neuroepithelium at the midbrain-hindbrain junction.1 Publication

Gene expression databases

BgeeiENSMUSG00000022997.
CleanExiMM_WNT1.
ExpressionAtlasiP04426. baseline and differential.
GenevisibleiP04426. MM.

Interactioni

Subunit structurei

Interacts with PORCN. Interacts with RSPO1, RSPO2 and RSPO3. Interacts with WLS.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
KlO350822EBI-1570911,EBI-1570828
LRP6O755812EBI-1570911,EBI-910915From a different organism.

GO - Molecular functioni

  • cytokine activity Source: BHF-UCL
  • frizzled binding Source: GO_Central
  • protein domain specific binding Source: UniProtKB
  • receptor agonist activity Source: ParkinsonsUK-UCL
  • receptor binding Source: MGI

Protein-protein interaction databases

BioGridi204567. 9 interactors.
DIPiDIP-39896N.
IntActiP04426. 2 interactors.
STRINGi10090.ENSMUSP00000023734.

Structurei

3D structure databases

ProteinModelPortaliP04426.
SMRiP04426.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Wnt family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3913. Eukaryota.
ENOG410XQZ1. LUCA.
GeneTreeiENSGT00760000118943.
HOGENOMiHOG000039528.
HOVERGENiHBG001595.
InParanoidiP04426.
KOiK03209.
OMAiNNSGRWW.
OrthoDBiEOG091G0OFF.
PhylomeDBiP04426.
TreeFamiTF105310.

Family and domain databases

InterProiIPR005817. Wnt.
IPR009139. Wnt1.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERiPTHR12027. PTHR12027. 1 hit.
PfamiPF00110. wnt. 1 hit.
[Graphical view]
PRINTSiPR01841. WNT1PROTEIN.
PR01349. WNTPROTEIN.
SMARTiSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEiPS00246. WNT1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04426-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLWALLPSW VSTTLLLALT ALPAALAANS SGRWWGIVNI ASSTNLLTDS
60 70 80 90 100
KSLQLVLEPS LQLLSRKQRR LIRQNPGILH SVSGGLQSAV RECKWQFRNR
110 120 130 140 150
RWNCPTAPGP HLFGKIVNRG CRETAFIFAI TSAGVTHSVA RSCSEGSIES
160 170 180 190 200
CTCDYRRRGP GGPDWHWGGC SDNIDFGRLF GREFVDSGEK GRDLRFLMNL
210 220 230 240 250
HNNEAGRTTV FSEMRQECKC HGMSGSCTVR TCWMRLPTLR AVGDVLRDRF
260 270 280 290 300
DGASRVLYGN RGSNRASRAE LLRLEPEDPA HKPPSPHDLV YFEKSPNFCT
310 320 330 340 350
YSGRLGTAGT AGRACNSSSP ALDGCELLCC GRGHRTRTQR VTERCNCTFH
360 370
WCCHVSCRNC THTRVLHECL
Length:370
Mass (Da):41,086
Last modified:August 13, 1987 - v1
Checksum:i02EEB23109231A40
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02593 Genomic DNA. Translation: AAA39321.1.
M11943 mRNA. Translation: AAA39322.1.
BC005449 mRNA. Translation: AAH05449.1.
CCDSiCCDS27807.1.
PIRiA23447. TVMST1.
RefSeqiNP_067254.1. NM_021279.4.
UniGeneiMm.1123.

Genome annotation databases

EnsembliENSMUST00000023734; ENSMUSP00000023734; ENSMUSG00000022997.
GeneIDi22408.
KEGGimmu:22408.
UCSCiuc007xnx.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02593 Genomic DNA. Translation: AAA39321.1.
M11943 mRNA. Translation: AAA39322.1.
BC005449 mRNA. Translation: AAH05449.1.
CCDSiCCDS27807.1.
PIRiA23447. TVMST1.
RefSeqiNP_067254.1. NM_021279.4.
UniGeneiMm.1123.

3D structure databases

ProteinModelPortaliP04426.
SMRiP04426.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204567. 9 interactors.
DIPiDIP-39896N.
IntActiP04426. 2 interactors.
STRINGi10090.ENSMUSP00000023734.

PTM databases

PhosphoSitePlusiP04426.

Proteomic databases

PaxDbiP04426.
PRIDEiP04426.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023734; ENSMUSP00000023734; ENSMUSG00000022997.
GeneIDi22408.
KEGGimmu:22408.
UCSCiuc007xnx.1. mouse.

Organism-specific databases

CTDi7471.
MGIiMGI:98953. Wnt1.

Phylogenomic databases

eggNOGiKOG3913. Eukaryota.
ENOG410XQZ1. LUCA.
GeneTreeiENSGT00760000118943.
HOGENOMiHOG000039528.
HOVERGENiHBG001595.
InParanoidiP04426.
KOiK03209.
OMAiNNSGRWW.
OrthoDBiEOG091G0OFF.
PhylomeDBiP04426.
TreeFamiTF105310.

Enzyme and pathway databases

ReactomeiR-MMU-3238698. WNT ligand biogenesis and trafficking.
R-MMU-381340. Transcriptional regulation of white adipocyte differentiation.
R-MMU-4086400. PCP/CE pathway.
R-MMU-442533. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.
R-MMU-4641262. Disassembly of the destruction complex and recruitment of AXIN to the membrane.

Miscellaneous databases

PROiP04426.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022997.
CleanExiMM_WNT1.
ExpressionAtlasiP04426. baseline and differential.
GenevisibleiP04426. MM.

Family and domain databases

InterProiIPR005817. Wnt.
IPR009139. Wnt1.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERiPTHR12027. PTHR12027. 1 hit.
PfamiPF00110. wnt. 1 hit.
[Graphical view]
PRINTSiPR01841. WNT1PROTEIN.
PR01349. WNTPROTEIN.
SMARTiSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEiPS00246. WNT1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWNT1_MOUSE
AccessioniPrimary (citable) accession number: P04426
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: November 30, 2016
This is version 156 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Many mouse mammary tumors induced by mouse mammary tumor virus (MMTV) contain a provirus integrated into a host cell region which has been named INT-1 (now Wnt1).1 Publication

Caution

A palmitoylation site was proposed at Cys-93, but it was later shown that this cysteine is engaged in a disulfide bond.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.