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P04426

- WNT1_MOUSE

UniProt

P04426 - WNT1_MOUSE

Protein

Proto-oncogene Wnt-1

Gene

Wnt1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 1 (13 Aug 1987)
      Previous versions | rss
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    Functioni

    Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters. Proeminent role in the induction of the mesencephalon and cerebellum. May play a crucial role in the morphogenesis of the neural tube and/or the early stages of CNS development. Has a role in osteoblast function and bone development By similarity.By similarity

    GO - Molecular functioni

    1. cytokine activity Source: BHF-UCL
    2. frizzled binding Source: RefGenome
    3. protein binding Source: UniProtKB
    4. protein domain specific binding Source: UniProtKB
    5. receptor binding Source: MGI
    6. transcription regulatory region DNA binding Source: Ensembl

    GO - Biological processi

    1. BMP signaling pathway Source: MGI
    2. bone development Source: UniProtKB
    3. branching involved in ureteric bud morphogenesis Source: MGI
    4. canonical Wnt signaling pathway Source: BHF-UCL
    5. canonical Wnt signaling pathway involved in negative regulation of apoptotic process Source: Ensembl
    6. cell-cell signaling Source: BHF-UCL
    7. cellular response to peptide hormone stimulus Source: Ensembl
    8. central nervous system morphogenesis Source: BHF-UCL
    9. cerebellum formation Source: BHF-UCL
    10. diencephalon development Source: MGI
    11. embryonic axis specification Source: BHF-UCL
    12. forebrain anterior/posterior pattern specification Source: MGI
    13. hematopoietic stem cell proliferation Source: MGI
    14. hepatocyte differentiation Source: Ensembl
    15. inner ear morphogenesis Source: MGI
    16. metencephalon development Source: MGI
    17. midbrain development Source: BHF-UCL
    18. midbrain-hindbrain boundary development Source: MGI
    19. midbrain-hindbrain boundary maturation during brain development Source: MGI
    20. myoblast fusion Source: MGI
    21. myotube differentiation Source: MGI
    22. negative regulation of BMP signaling pathway Source: Ensembl
    23. negative regulation of cell aging Source: Ensembl
    24. negative regulation of cell-cell adhesion Source: Ensembl
    25. negative regulation of cell differentiation Source: AgBase
    26. negative regulation of cell-substrate adhesion Source: Ensembl
    27. negative regulation of fat cell differentiation Source: BHF-UCL
    28. negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: MGI
    29. negative regulation of transforming growth factor beta receptor signaling pathway Source: MGI
    30. neuron fate commitment Source: MGI
    31. neuron fate determination Source: MGI
    32. organ morphogenesis Source: MGI
    33. organ regeneration Source: Ensembl
    34. positive regulation of cell proliferation Source: MGI
    35. positive regulation of dermatome development Source: Ensembl
    36. positive regulation of fibroblast proliferation Source: Ensembl
    37. positive regulation of insulin-like growth factor receptor signaling pathway Source: Ensembl
    38. positive regulation of lamellipodium assembly Source: Ensembl
    39. positive regulation of Notch signaling pathway Source: Ensembl
    40. positive regulation of protein phosphorylation Source: MGI
    41. positive regulation of sequence-specific DNA binding transcription factor activity Source: BHF-UCL
    42. positive regulation of transcription, DNA-templated Source: UniProtKB
    43. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    44. response to wounding Source: Ensembl
    45. signal transduction Source: MGI
    46. signal transduction in response to DNA damage Source: Ensembl
    47. Spemann organizer formation Source: BHF-UCL
    48. spinal cord association neuron differentiation Source: MGI
    49. T cell differentiation in thymus Source: MGI
    50. ubiquitin-dependent SMAD protein catabolic process Source: MGI
    51. Wnt signaling pathway Source: MGI

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Wnt signaling pathway

    Enzyme and pathway databases

    ReactomeiREACT_207044. TCF dependent signaling in response to WNT.
    REACT_213918. WNT ligand biogenesis and trafficking.
    REACT_214043. PCP/CE pathway.
    REACT_216784. disassembly of the destruction complex and recruitment of AXIN to the membrane.
    REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proto-oncogene Wnt-1
    Alternative name(s):
    Proto-oncogene Int-1
    Gene namesi
    Name:Wnt1
    Synonyms:Int-1, Wnt-1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:98953. Wnt1.

    Subcellular locationi

    GO - Cellular componenti

    1. cell surface Source: MGI
    2. endoplasmic reticulum lumen Source: Reactome
    3. extracellular space Source: RefGenome
    4. proteinaceous extracellular matrix Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Extracellular matrix, Secreted

    Pathology & Biotechi

    Involvement in diseasei

    Many mouse mammary tumors induced by mouse mammary tumor virus (MMTV) contain a provirus integrated into a host cell region which has been named INT-1 (now Wnt1).

    Keywords - Diseasei

    Proto-oncogene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Chaini28 – 370343Proto-oncogene Wnt-1PRO_0000041406Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi29 – 291N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi93 ↔ 104By similarity
    Disulfide bondi143 ↔ 151By similarity
    Disulfide bondi153 ↔ 170By similarity
    Disulfide bondi218 ↔ 232By similarity
    Disulfide bondi220 ↔ 227By similarity
    Lipidationi224 – 2241O-palmitoyl serine; by PORCNBy similarity
    Disulfide bondi315 ↔ 330By similarity
    Glycosylationi316 – 3161N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi345 ↔ 360By similarity
    Glycosylationi346 – 3461N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi347 ↔ 357By similarity
    Disulfide bondi352 ↔ 353By similarity
    Glycosylationi359 – 3591N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Palmitoylation at Ser-224 is required for efficient binding to frizzled receptors. Palmitoylation is necessary for proper trafficking to cell surface. A palmitoylation site was proposed at Cys-93, but it was later shown that this cysteine is engaged in a disulfide bond By similarity.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

    Proteomic databases

    PRIDEiP04426.

    PTM databases

    PhosphoSiteiP04426.

    Expressioni

    Tissue specificityi

    Testis and mid-gestational embryos. In the testis, detected only in postmeiotic germ cells undergoing differentiation from round spermatids into mature spermatozoa. In the embryos, expression is restricted to the developing CNS in regions of the neural tube other than the telencephalon. Expressed in osteoblast; expression levels increase with advancing osteoblast differentiation. Expressed in the brain, femur, spleen, and hematopoietic bone marrow.3 Publications

    Developmental stagei

    Accumulates throughout the neural plate at the anterior head folds of the 9 day embryo but only at its lateral tips in more posterior regions. Following neural tube closure, expression is restricted to specific regions of the dorsal wall of the brain ventricles and spinal cord, the ventral wall of the midbrain and the diencephalon, and the lateral walls of the neuroepithelium at the midbrain-hindbrain junction.1 Publication

    Gene expression databases

    ArrayExpressiP04426.
    BgeeiP04426.
    CleanExiMM_WNT1.
    GenevestigatoriP04426.

    Interactioni

    Subunit structurei

    Interacts with PORCN. Interacts with RSPO1, RSPO2 and RSPO3. Interacts with WLS.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    KlO350822EBI-1570911,EBI-1570828
    LRP6O755812EBI-1570911,EBI-910915From a different organism.

    Protein-protein interaction databases

    BioGridi204567. 9 interactions.
    DIPiDIP-39896N.
    IntActiP04426. 2 interactions.
    STRINGi10090.ENSMUSP00000023734.

    Structurei

    3D structure databases

    ProteinModelPortaliP04426.
    SMRiP04426. Positions 85-299.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the Wnt family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG284879.
    HOGENOMiHOG000039528.
    HOVERGENiHBG001595.
    InParanoidiP04426.
    KOiK03209.
    OMAiAIKECKW.
    OrthoDBiEOG7C8GJ8.
    PhylomeDBiP04426.
    TreeFamiTF105310.

    Family and domain databases

    InterProiIPR005817. Wnt.
    IPR009139. Wnt1.
    IPR018161. Wnt_CS.
    [Graphical view]
    PANTHERiPTHR12027. PTHR12027. 1 hit.
    PfamiPF00110. wnt. 1 hit.
    [Graphical view]
    PRINTSiPR01841. WNT1PROTEIN.
    PR01349. WNTPROTEIN.
    SMARTiSM00097. WNT1. 1 hit.
    [Graphical view]
    PROSITEiPS00246. WNT1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P04426-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLWALLPSW VSTTLLLALT ALPAALAANS SGRWWGIVNI ASSTNLLTDS    50
    KSLQLVLEPS LQLLSRKQRR LIRQNPGILH SVSGGLQSAV RECKWQFRNR 100
    RWNCPTAPGP HLFGKIVNRG CRETAFIFAI TSAGVTHSVA RSCSEGSIES 150
    CTCDYRRRGP GGPDWHWGGC SDNIDFGRLF GREFVDSGEK GRDLRFLMNL 200
    HNNEAGRTTV FSEMRQECKC HGMSGSCTVR TCWMRLPTLR AVGDVLRDRF 250
    DGASRVLYGN RGSNRASRAE LLRLEPEDPA HKPPSPHDLV YFEKSPNFCT 300
    YSGRLGTAGT AGRACNSSSP ALDGCELLCC GRGHRTRTQR VTERCNCTFH 350
    WCCHVSCRNC THTRVLHECL 370
    Length:370
    Mass (Da):41,086
    Last modified:August 13, 1987 - v1
    Checksum:i02EEB23109231A40
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K02593 Genomic DNA. Translation: AAA39321.1.
    M11943 mRNA. Translation: AAA39322.1.
    BC005449 mRNA. Translation: AAH05449.1.
    CCDSiCCDS27807.1.
    PIRiA23447. TVMST1.
    RefSeqiNP_067254.1. NM_021279.4.
    XP_006520953.1. XM_006520890.1.
    UniGeneiMm.1123.

    Genome annotation databases

    EnsembliENSMUST00000023734; ENSMUSP00000023734; ENSMUSG00000022997.
    GeneIDi22408.
    KEGGimmu:22408.
    UCSCiuc007xnx.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K02593 Genomic DNA. Translation: AAA39321.1 .
    M11943 mRNA. Translation: AAA39322.1 .
    BC005449 mRNA. Translation: AAH05449.1 .
    CCDSi CCDS27807.1.
    PIRi A23447. TVMST1.
    RefSeqi NP_067254.1. NM_021279.4.
    XP_006520953.1. XM_006520890.1.
    UniGenei Mm.1123.

    3D structure databases

    ProteinModelPortali P04426.
    SMRi P04426. Positions 85-299.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204567. 9 interactions.
    DIPi DIP-39896N.
    IntActi P04426. 2 interactions.
    STRINGi 10090.ENSMUSP00000023734.

    PTM databases

    PhosphoSitei P04426.

    Proteomic databases

    PRIDEi P04426.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000023734 ; ENSMUSP00000023734 ; ENSMUSG00000022997 .
    GeneIDi 22408.
    KEGGi mmu:22408.
    UCSCi uc007xnx.1. mouse.

    Organism-specific databases

    CTDi 7471.
    MGIi MGI:98953. Wnt1.

    Phylogenomic databases

    eggNOGi NOG284879.
    HOGENOMi HOG000039528.
    HOVERGENi HBG001595.
    InParanoidi P04426.
    KOi K03209.
    OMAi AIKECKW.
    OrthoDBi EOG7C8GJ8.
    PhylomeDBi P04426.
    TreeFami TF105310.

    Enzyme and pathway databases

    Reactomei REACT_207044. TCF dependent signaling in response to WNT.
    REACT_213918. WNT ligand biogenesis and trafficking.
    REACT_214043. PCP/CE pathway.
    REACT_216784. disassembly of the destruction complex and recruitment of AXIN to the membrane.
    REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

    Miscellaneous databases

    NextBioi 302801.
    PROi P04426.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P04426.
    Bgeei P04426.
    CleanExi MM_WNT1.
    Genevestigatori P04426.

    Family and domain databases

    InterProi IPR005817. Wnt.
    IPR009139. Wnt1.
    IPR018161. Wnt_CS.
    [Graphical view ]
    PANTHERi PTHR12027. PTHR12027. 1 hit.
    Pfami PF00110. wnt. 1 hit.
    [Graphical view ]
    PRINTSi PR01841. WNT1PROTEIN.
    PR01349. WNTPROTEIN.
    SMARTi SM00097. WNT1. 1 hit.
    [Graphical view ]
    PROSITEi PS00246. WNT1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and nucleotide sequence of the putative mammary oncogene int-1; proviral insertions leave the protein-encoding domain intact."
      Ooyen A.V., Nusse R.
      Cell 39:233-240(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: C3H.
    2. "Nucleotide sequence and expression in vitro of cDNA derived from mRNA of int-1, a provirally activated mouse mammary oncogene."
      Fung Y.-K.T., Shackleford G.M., Brown A.M.C., Sanders G.S., Varmus H.E.
      Mol. Cell. Biol. 5:3337-3344(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Expression of multiple novel Wnt-1/int-1-related genes during fetal and adult mouse development."
      Gavin B.J., McMahon J.A., McMahon A.P.
      Genes Dev. 4:2319-2332(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Czech II.
    5. "Targeted disruption of the murine int-1 proto-oncogene resulting in severe abnormalities in midbrain and cerebellar development."
      Thomas K.R., Capecchi M.R.
      Nature 346:847-850(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: POSSIBLE FUNCTION.
    6. "Expression of the proto-oncogene int-1 is restricted to specific neural cells in the developing mouse embryo."
      Wilkinson D.G., Bailes J.A., McMahon A.P.
      Cell 50:79-88(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
      Strain: CBA/Ca.
      Tissue: Embryo.
    7. "Expression of the proto-oncogene int-1 is restricted to postmeiotic male germ cells and the neural tube of mid-gestational embryos."
      Shackleford G.M., Varmus H.E.
      Cell 50:89-95(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
      Strain: ICR.
    8. "The evolutionarily conserved porcupine gene family is involved in the processing of the Wnt family."
      Tanaka K., Okabayashi H., Asashima M., Perrimon N., Kadowaki T.
      Eur. J. Biochem. 267:4300-4311(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PORCN.
    9. "Mammalian Ryk is a Wnt coreceptor required for stimulation of neurite outgrowth."
      Lu W., Yamamoto V., Ortega B., Baltimore D.
      Cell 119:97-108(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "Ryk-mediated Wnt repulsion regulates posterior-directed growth of corticospinal tract."
      Liu Y., Shi J., Lu C.C., Wang Z.B., Lyuksyutova A.I., Song X.J., Zou Y.
      Nat. Neurosci. 8:1151-1159(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "Mouse cristin/R-spondin family proteins are novel ligands for the Frizzled 8 and LRP6 receptors and activate beta-catenin-dependent gene expression."
      Nam J.-S., Turcotte T.J., Smith P.F., Choi S., Yoon J.K.
      J. Biol. Chem. 281:13247-13257(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RSPO1; RSPO2 AND RSPO3.
    12. "Reciprocal regulation of Wnt and Gpr177/mouse Wntless is required for embryonic axis formation."
      Fu J., Jiang M., Mirando A.J., Yu H.-M., Hsu W.
      Proc. Natl. Acad. Sci. U.S.A. 106:18598-18603(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH WLS.
    13. Cited for: TISSUE SPECIFICITY.
    14. Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiWNT1_MOUSE
    AccessioniPrimary (citable) accession number: P04426
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: August 13, 1987
    Last modified: October 1, 2014
    This is version 134 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3