P04426 (WNT1_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified
April 3, 2013.
Version 120.
History...
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Proto-oncogene Wnt-1 Alternative name(s): Proto-oncogene Int-1 | ||||
| Gene names |
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| Organism | Mus musculus (Mouse) [Reference proteome] | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus![]() |
Protein attributes
| Sequence length | 370 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters. Proeminent role in the induction of the mesencephalon and cerebellum. May play a crucial role in the morphogenesis of the neural tube and/or the early stages of CNS development. Ref.5 Ref.9 Ref.10 |
| Subunit structure | Interacts with PORCN. Interacts with RSPO1, RSPO2 and RSPO3. Interacts with WLS. Ref.8 Ref.11 Ref.12 |
| Subcellular location | |
| Tissue specificity | Testis and mid-gestational embryos. In the testis, detected only in postmeiotic germ cells undergoing differentiation from round spermatids into mature spermatozoa. In the embryos, expression is restricted to the developing CNS in regions of the neural tube other than the telencephalon. Ref.7 |
| Developmental stage | Accumulates throughout the neural plate at the anterior head folds of the 9 day embryo but only at its lateral tips in more posterior regions. Following neural tube closure, expression is restricted to specific regions of the dorsal wall of the brain ventricles and spinal cord, the ventral wall of the midbrain and the diencephalon, and the lateral walls of the neuroepithelium at the midbrain-hindbrain junction. Ref.6 |
| Post-translational modification | Palmitoylation at Ser-224 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-93. Palmitoylation is necessary for proper trafficking to cell surface By similarity. |
| Involvement in disease | Many mouse mammary tumors induced by mouse mammary tumor virus (MMTV) contain a provirus integrated into a host cell region which has been named INT-1 (now Wnt1). |
| Sequence similarities | Belongs to the Wnt family. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| Kl | O35082 | 2 | EBI-1570911,EBI-1570828 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 27 | 27 | Potential | ||||||
| Chain | 28 – 370 | 343 | Proto-oncogene Wnt-1 | PRO_0000041406 | |||||
Amino acid modifications | |||||||||
| Lipidation | 93 | 1 | S-palmitoyl cysteine By similarity | ||||||
| Lipidation | 224 | 1 | O-palmitoyl serine; by PORCN By similarity | ||||||
| Glycosylation | 29 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 316 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 346 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 359 | 1 | N-linked (GlcNAc...) Potential | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Structure and nucleotide sequence of the putative mammary oncogene int-1; proviral insertions leave the protein-encoding domain intact." Ooyen A.V., Nusse R. Cell 39:233-240(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C3H. |
| [2] | "Nucleotide sequence and expression in vitro of cDNA derived from mRNA of int-1, a provirally activated mouse mammary oncogene." Fung Y.-K.T., Shackleford G.M., Brown A.M.C., Sanders G.S., Varmus H.E. Mol. Cell. Biol. 5:3337-3344(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [3] | "Expression of multiple novel Wnt-1/int-1-related genes during fetal and adult mouse development." Gavin B.J., McMahon J.A., McMahon A.P. Genes Dev. 4:2319-2332(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II. |
| [5] | "Targeted disruption of the murine int-1 proto-oncogene resulting in severe abnormalities in midbrain and cerebellar development." Thomas K.R., Capecchi M.R. Nature 346:847-850(1990) [PubMed] [Europe PMC] [Abstract] Cited for: POSSIBLE FUNCTION. |
| [6] | "Expression of the proto-oncogene int-1 is restricted to specific neural cells in the developing mouse embryo." Wilkinson D.G., Bailes J.A., McMahon A.P. Cell 50:79-88(1987) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE. Strain: CBA/Ca. Tissue: Embryo. |
| [7] | "Expression of the proto-oncogene int-1 is restricted to postmeiotic male germ cells and the neural tube of mid-gestational embryos." Shackleford G.M., Varmus H.E. Cell 50:89-95(1987) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY. Strain: ICR. |
| [8] | "The evolutionarily conserved porcupine gene family is involved in the processing of the Wnt family." Tanaka K., Okabayashi H., Asashima M., Perrimon N., Kadowaki T. Eur. J. Biochem. 267:4300-4311(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PORCN. |
| [9] | "Mammalian Ryk is a Wnt coreceptor required for stimulation of neurite outgrowth." Lu W., Yamamoto V., Ortega B., Baltimore D. Cell 119:97-108(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. |
| [10] | "Ryk-mediated Wnt repulsion regulates posterior-directed growth of corticospinal tract." Liu Y., Shi J., Lu C.C., Wang Z.B., Lyuksyutova A.I., Song X.J., Zou Y. Nat. Neurosci. 8:1151-1159(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. |
| [11] | "Mouse cristin/R-spondin family proteins are novel ligands for the Frizzled 8 and LRP6 receptors and activate beta-catenin-dependent gene expression." Nam J.-S., Turcotte T.J., Smith P.F., Choi S., Yoon J.K. J. Biol. Chem. 281:13247-13257(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RSPO1; RSPO2 AND RSPO3. |
| [12] | "Reciprocal regulation of Wnt and Gpr177/mouse Wntless is required for embryonic axis formation." Fu J., Jiang M., Mirando A.J., Yu H.-M., Hsu W. Proc. Natl. Acad. Sci. U.S.A. 106:18598-18603(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH WLS. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | K02593 Genomic DNA. Translation: AAA39321.1. M11943 mRNA. Translation: AAA39322.1. BC005449 mRNA. Translation: AAH05449.1. |
| IPI | IPI00115819. |
| PIR | TVMST1. A23447. |
| RefSeq | NP_067254.1. NM_021279.4. |
| UniGene | Mm.1123. |
3D structure databases | |
| ProteinModelPortal | P04426. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-39896N. |
| IntAct | P04426. 1 interaction. |
| STRING | 10090.ENSMUSP00000023734. |
PTM databases | |
| PhosphoSite | P04426. |
Proteomic databases | |
| PRIDE | P04426. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSMUST00000023734; ENSMUSP00000023734; ENSMUSG00000022997. |
| GeneID | 22408. |
| KEGG | mmu:22408. |
Organism-specific databases | |
| CTD | 7471. |
| MGI | MGI:98953. Wnt1. |
Phylogenomic databases | |
| eggNOG | NOG284879. |
| HOGENOM | HOG000039528. |
| HOVERGEN | HBG001595. |
| InParanoid | P04426. |
| KO | K03209. |
| OMA | AIKECKW. |
| OrthoDB | EOG4R23V2. |
Gene expression databases | |
| ArrayExpress | P04426. |
| Bgee | P04426. |
| CleanEx | MM_WNT1. |
| Genevestigator | P04426. |
| GermOnline | ENSMUSG00000022997. Mus musculus. |
Family and domain databases | |
| InterPro | IPR005817. Wnt. IPR009139. Wnt1. IPR018161. Wnt_CS. [Graphical view] |
| PANTHER | PTHR12027. PTHR12027. 1 hit. PTHR12027:SF15. PTHR12027:SF15. 1 hit. |
| Pfam | PF00110. wnt. 1 hit. [Graphical view] |
| PRINTS | PR01841. WNT1PROTEIN. PR01349. WNTPROTEIN. |
| SMART | SM00097. WNT1. 1 hit. [Graphical view] |
| PROSITE | PS00246. WNT1. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 302801. |
| SOURCE | Search... |
Entry information
| Entry name | WNT1_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P04426 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with
