Reviewed, UniProtKB/Swiss-Prot P04421 (LYSC_BOVIN)

Names and origin

Protein names

Recommended name:
    Lysozyme C
    EC=3.2.1.17
Alternative name(s):
    1,4-beta-N-acetylmuramidase C

Gene names

Name:

LYZ1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Subunit structure	Monomer.
Tissue specificity	Stomach-specific.
Miscellaneous	Lysozyme C is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. It acts rapidly on both peptide-substituted and unsubstituted peptidoglycan, and slowly on chitin oligosaccharides. The ruminant gastric lysozymes, which digest symbiotic bacteria coming with cud from the rumen, are much more resistant to inactivation by pepsin than are other lysozymes. Three non-allelic lysozymes C are present in the gastric mucosa of cattle. The sequence of isozyme 2B is shown.
Sequence similarities	Belongs to the glycosyl hydrolase 22 family.

Ontologies

Keywords
Biological process	Digestion
Domain	Signal
Molecular function	Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW digestion Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	lysozyme activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Ref.2
	Chain	19 – 147	129	Lysozyme C		PRO_0000018453
Sites
	Active site	53	1	By similarity
	Active site	71	1	By similarity
Amino acid modifications
	Disulfide bond	24 ↔ 145		By similarity
	Disulfide bond	48 ↔ 133		By similarity
	Disulfide bond	83 ↔ 99		By similarity
	Disulfide bond	95 ↔ 113		By similarity
Natural variations
	Natural variant	3	1	A → T in isozyme 2A.
	Natural variant	5	1	V → I in isozyme 1 and isozyme 3.
	Natural variant	17	1	Q → K in isozyme 2C.
	Natural variant	45	1	N → S in isozyme 2D.
	Natural variant	66	1	S → G in isozyme 1.
	Natural variant	106	1	D → E in isozyme 1.
	Natural variant	116	1	H → Q in isozyme 1.
	Natural variant	143	1	E → Q in isozyme 3.

Sequences

	Sequence		Length	Mass (Da)
	P04421-1 [UniParc]. Last modified October 1, 1989. Version 2. Checksum: C678E7EECE1E66BF	FASTA	147	16,373
	10 20 30 40 50 60 MKALVILGFL FLSVAVQGKV FERCELARTL KKLGLDGYKG VSLANWLCLT KWESSYNTKA 70 80 90 100 110 120 TNYNPSSEST DYGIFQINSK WWCNDGKTPN AVDGCHVSCR ELMENDIAKA VACAKHIVSE 130 140 QGITAWVAWK SHCRDHDVSS YVEGCTL

References

[1]	"Multiple cDNA sequences and the evolution of bovine stomach lysozyme." Irwin D.M., Wilson A.C. J. Biol. Chem. 264:11387-11393(1989) [PubMed: 2738070] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Stomach lysozymes of ruminants. II. Amino acid sequence of cow lysozyme 2 and immunological comparisons with other lysozymes." Jolles P., Schoentgen F., Jolles J., Dobson D.E., Prager E.M., Wilson A.C. J. Biol. Chem. 259:11617-11625(1984) [PubMed: 6470012] [Abstract] Cited for: PROTEIN SEQUENCE OF 19-147 (2B).
[3]	"Episodic evolution in the stomach lysozymes of ruminants." Jolles J., Jolles P., Bowman B.H., Prager E.M., Stewart C.-B., Wilson A.C. J. Mol. Evol. 28:528-535(1989) [PubMed: 2504928] [Abstract] Cited for: SEQUENCE REVISION TO 116 (2B).

Cross-references

Sequence databases
EMBLGenBankDDBJ	M26246 mRNA. Translation: AAA30627.1. M26245 mRNA. Translation: AAA30626.1. M26242 mRNA. Translation: AAA30632.1. M26241 mRNA. Translation: AAA30628.1. M26243 mRNA. Translation: AAA30629.1. M26244 mRNA. Translation: AAA30630.1. M26240 mRNA. Translation: AAA30631.1.
IPI	IPI00694099.
RefSeq	NP_001073808.1. NP_776529.1.
UniGene	Bt.29367 Bt.80498 Bt.89770
3D structure databases
HSSP	HSSP built from PDB template 1LZ6 based on UniProtKB P00695.
ModBase	Search...
Protein family/group databases
CAZy	GH22. Glycoside Hydrolase Family 22.
Genome annotation databases
Ensembl	ENSBTAG00000026088. Bos taurus. [Contig view]
GeneID	281289. 781349.
KEGG	bta:281289. bta:781349.
Phylogenomic databases
HOVERGEN	P04421.
Enzyme and pathway databases
BRENDA	3.2.1.17. 251.
Family and domain databases
InterPro	IPR001916. Glyco_hydro_22. IPR019799. Glyco_hydro_22_CS. IPR000974. Glyco_hydro_22_lys. [Graphical view]
Pfam	PF00062. Lys. 1 hit. [Graphical view]
PRINTS	PR00137. LYSOZYME. PR00135. LYZLACT.
SMART	SM00263. LYZ1. 1 hit. [Graphical view]
PROSITE	PS00128. LACTALBUMIN_LYSOZYME_1. 1 hit. PS51348. LACTALBUMIN_LYSOZYME_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LYSC_BOVIN

Accession

Primary (citable) accession number: P04421

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	October 1, 1989
Last modified:	June 16, 2009
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)