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Protein

Serine/threonine-protein kinase US3

Gene

US3

Organism
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Multifunctional serine/threonine kinase that plays a role in several processes including egress of virus particles from the nucleus, modulation of the actin cytoskeleton and inhibition of apoptosis. Phosphorylates UL31 and UL34, two critical regulators of capsid budding from nucleus to endoplasmic reticulum, thereby facilitating virion egress. Modulates and redistributes host components of the nuclear envelope, including LMNA, emerin/EMD and the nuclear matrix protein MATR3. Phosphorylates envelope glycoprotein B (gB), probably to direct it to the cell surface. Promotes virus intracellular spread by restructuring host cell cytoskeleton. Blocks host apoptosis to extend cell survival and allow efficient viral replication. Promotes viral gene expression by phosphorylating host HDAC2 to reduce viral genome silencing.8 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei220ATPPROSITE-ProRule annotation1
Active sitei305Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi197 – 205ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Host-virus interaction, Modulation of host cell apoptosis by virus, Modulation of host chromatin by virus

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase US3 (EC:2.7.11.1)
Gene namesi
Name:US3
OrganismiHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Taxonomic identifieri10299 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000009294 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

  • host cell cytoplasm Source: UniProtKB-SubCell
  • host cell nucleus Source: UniProtKB-SubCell
  • viral tegument Source: CACAO
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi147S → A: Complete loss of autophosphorylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000861801 – 481Serine/threonine-protein kinase US3Add BLAST481

Post-translational modificationi

Phosphorylated by UL13; this phosphorylation regulates subsequent phosphorylation of UL31 and UL34 by US3. Autophosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP04413.

PTM databases

iPTMnetiP04413.

Interactioni

Protein-protein interaction databases

BioGridi971434. 32 interactors.
IntActiP04413. 29 interactors.

Structurei

3D structure databases

ProteinModelPortaliP04413.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini191 – 478Protein kinasePROSITE-ProRule annotationAdd BLAST288

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

KOiK19439.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04413-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MACRKFCRVY GGQGRRKEEA VPPETKPSRV FPHGPFYTPA EDACLDSPPP
60 70 80 90 100
ETPKPSHTTP PSEAERLCHL QEILAQMYGN QDYPIEDDPS ADAADDVDED
110 120 130 140 150
APDDVAYPEE YAEELFLPGD ATGPLIGAND HIPPPCGASP PGIRRRSRDE
160 170 180 190 200
IGATGFTAEE LDAMDREAAR AISRGGKPPS TMAKLVTGMG FTIHGALTPG
210 220 230 240 250
SEGCVFDSSH PDYPQRVIVK AGWYTSTSHE ARLLRRLDHP AILPLLDLHV
260 270 280 290 300
VSGVTCLVLP KYQADLYTYL SRRLNPLGRP QIAAVSRQLL SAVDYIHRQG
310 320 330 340 350
IIHRDIKTEN IFINTPEDIC LGDFGAACFV QGSRSSPFPY GIAGTIDTNA
360 370 380 390 400
PEVLAGDPYT TTVDIWSAGL VIFETAVHNA SLFSAPRGPK RGPCDSQITR
410 420 430 440 450
IIRQAQVHVD EFSPHPESRL TSRYRSRAAG NNRPPYTRPA WTRYYKMDID
460 470 480
VEYLVCKALT FDGALRPSAA ELLCLPLFQQ K
Length:481
Mass (Da):52,835
Last modified:August 13, 1987 - v1
Checksum:i71838AB0483BE055
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00036 Genomic DNA. Translation: AAA96685.1.
X14112 Genomic DNA. Translation: CAA32280.1.
X02138 Genomic DNA. Translation: CAA26057.1.
PIRiA00656. TVBE17.
RefSeqiYP_009137138.1. NC_001806.2.

Genome annotation databases

GeneIDi2703401.
KEGGivg:2703401.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00036 Genomic DNA. Translation: AAA96685.1.
X14112 Genomic DNA. Translation: CAA32280.1.
X02138 Genomic DNA. Translation: CAA26057.1.
PIRiA00656. TVBE17.
RefSeqiYP_009137138.1. NC_001806.2.

3D structure databases

ProteinModelPortaliP04413.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi971434. 32 interactors.
IntActiP04413. 29 interactors.

PTM databases

iPTMnetiP04413.

Proteomic databases

PRIDEiP04413.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2703401.
KEGGivg:2703401.

Phylogenomic databases

KOiK19439.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUS03_HHV11
AccessioniPrimary (citable) accession number: P04413
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: October 5, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.