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Protein

Thymidine kinase

Gene

TK

Organism
Saimiriine herpesvirus 1 (strain MV-5-4-PSL) (SaHV-1) (Marmoset herpesvirus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of the gamma-phospho group of ATP to thymidine to generate dTMP in the salvage pathway of pyrimidine synthesis. The dTMP serves as a substrate for DNA polymerase during viral DNA replication. Allows the virus to be reactivated and to grow in non-proliferative cells lacking a high concentration of phosphorylated nucleic acid precursors.UniRule annotation

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei46 – 461Proton acceptorUniRule annotation
Binding sitei64 – 641SubstrateUniRule annotation
Binding sitei88 – 881SubstrateUniRule annotation
Binding sitei184 – 1841ATPUniRule annotation
Binding sitei190 – 1901SubstrateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi17 – 248ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinaseUniRule annotation (EC:2.7.1.21UniRule annotation)
Gene namesi
Name:TKUniRule annotation
OrganismiSaimiriine herpesvirus 1 (strain MV-5-4-PSL) (SaHV-1) (Marmoset herpesvirus)
Taxonomic identifieri10353 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiCallithrix [TaxID: 9481]
Saimiri (squirrel monkeys) [TaxID: 9520]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 361361Thymidine kinasePRO_0000175078Add
BLAST

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP04408.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi337 – 3426Poly-Ala

Sequence similaritiesi

Belongs to the herpesviridae thymidine kinase family.UniRule annotation

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_04029. HSV_KITH. 1 hit.
InterProiIPR001889. Herpes_TK.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00693. Herpes_TK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P04408-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGTAGTSRI LRVYLDGPHG VGKSTTAEAL VARCEPRRPI RSMLQEPMAY
60 70 80 90 100
WRSTFASDAI TEIYDTQHRL DSNEITAAEA GAFMTSLQLH MGTPYALLEE
110 120 130 140 150
AMRPHVGREL AEPDDNGPLP QRRDFVLVVD RHAVASMVCY PLARFMMGCV
160 170 180 190 200
SLRSVASLIS HLPPPLPGTN LVVASLDFRE HAARLRARAR PGERLDLTMM
210 220 230 240 250
AAIRNAYAML ANTSRYLLSG GDWRRDWGSL PVFKPSAFVA RAAKTAYTLP
260 270 280 290 300
LRDEPGLADT LFAALKVPEF LDARGYPRAA HAWTLDILAN RIRALRVYTL
310 320 330 340 350
DLTGPPEACA AAFRRLCAGL VLTEGSHPGA LCELKRAAAA YAREMSVVGS
360
REPTTAEVES A
Length:361
Mass (Da):39,458
Last modified:November 1, 1995 - v2
Checksum:iB2E322DB1D5542DB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02122 Genomic DNA. Translation: AAA67102.1.
PIRiA00614. KIBETM.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02122 Genomic DNA. Translation: AAA67102.1.
PIRiA00614. KIBETM.

3D structure databases

ProteinModelPortaliP04408.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_04029. HSV_KITH. 1 hit.
InterProiIPR001889. Herpes_TK.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00693. Herpes_TK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKITH_SHV1
AccessioniPrimary (citable) accession number: P04408
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: November 1, 1995
Last modified: July 6, 2016
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.