Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P04407

- KITH_HHV23

UniProt

P04407 - KITH_HHV23

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Thymidine kinase

Gene

TK

Organism
Human herpesvirus 2 (strain 333) (HHV-2) (Human herpes simplex virus 2)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

In latent infection, may allow the virus to be reactivated and to grow in cells lacking a high concentration of phosphorylated nucleic acid precursors, such as nerve cells that do not replicate their genome.By similarity

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei84 – 841Proton acceptorSequence Analysis
Binding sitei102 – 1021SubstrateBy similarity
Binding sitei126 – 1261SubstrateBy similarity
Binding sitei173 – 1731SubstrateBy similarity
Binding sitei217 – 2171ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi56 – 638ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. thymidine kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
  2. TMP biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase (EC:2.7.1.21)
Gene namesi
Name:TK
OrganismiHuman herpesvirus 2 (strain 333) (HHV-2) (Human herpes simplex virus 2)
Taxonomic identifieri10313 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]

Pathology & Biotechi

Biotechnological usei

Used in molecular biology as a selectable marker to identify transfected eukaryotic cells. Used in cancer suicide gene therapy to selectively kill transformed cells.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 376376Thymidine kinasePRO_0000175074Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP04407.
SMRiP04407. Positions 46-374.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR001889. Herpes_TK.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00693. Herpes_TK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P04407-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASHAGQQHA PAFGQAARAS GPTDGRAASR PSHRQGASEA RGDPELPTLL
60 70 80 90 100
RVYIDGPHGV GKTTTSAQLM EALGPRDNIV YVPEPMTYWQ VLGASETLTN
110 120 130 140 150
IYNTQHRLDR GEISAGEAAV VMTSAQITMS TPYAATDAVL APHIGGEAVG
160 170 180 190 200
PQAPPPALTL VFDRHPIASL LCYPAARYLM GSMTPQAVLA FVALMPPTAP
210 220 230 240 250
GTNLVLGVLP EAEHADRLAR RQRPGERLDL AMLSAIRRVY DLLANTVRYL
260 270 280 290 300
QRGGRWREDW GRLTGVAAAT PRPDPEDGAG SLPRIEDTLF ALFRVPELLA
310 320 330 340 350
PNGDLYHIFA WVLDVLADRL LPMHLFVLDY DQSPVGCRDA LLRLTAGMIP
360 370
TRVTTAGSIA EIRDLARTFA REVGGV
Length:376
Mass (Da):40,467
Last modified:December 15, 1998 - v2
Checksum:i0B1D68B0BBA3C8A5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti271 – 2711Missing in CAA25858. (PubMed:6317035)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01712 Genomic DNA. Translation: CAA25858.1.
V00466 Genomic DNA. Translation: CAA23740.1.
M29942 Genomic DNA. Translation: AAA45856.1.
PIRiA90648. KIBET3.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01712 Genomic DNA. Translation: CAA25858.1 .
V00466 Genomic DNA. Translation: CAA23740.1 .
M29942 Genomic DNA. Translation: AAA45856.1 .
PIRi A90648. KIBET3.

3D structure databases

ProteinModelPortali P04407.
SMRi P04407. Positions 46-374.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P04407.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR001889. Herpes_TK.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00693. Herpes_TK. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the herpes simplex virus type 2 (HSV-2) thymidine kinase gene and predicted amino acid sequence of thymidine kinase polypeptide and its comparison with the HSV-1 thymidine kinase gene."
    Kit S., Kit M., Qavi H., Trkula D., Otsuka H.
    Biochim. Biophys. Acta 741:158-170(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Nucleotide sequence of the herpes simplex virus type 2 thymidine kinase gene."
    Swain M.A., Galloway D.A.
    J. Virol. 46:1045-1050(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Nucleotide sequence changes in thymidine kinase gene of herpes simplex virus type 2 clones from an isolate of a patient treated with acyclovir."
    Kit S., Sheppard M., Ichimura H., Nusinoff-Lehrman S., Ellis M.N., Fyfe J.A., Otsuka H.
    Antimicrob. Agents Chemother. 31:1483-1490(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiKITH_HHV23
AccessioniPrimary (citable) accession number: P04407
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: December 15, 1998
Last modified: October 1, 2014
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Phosphorylates and thereby activates certain drugs like acyclovir (ACV), valaciclovir, and famciclovir to a toxic form, that leads to successful suppression of the infection, while the uninfected cell does not have this ability because it lacks TK. Mutations in thymidine kinase may induce HSV resistance to antiviral therapies in immunocompromised patients. The most frequently observed resistant strains are unable to express TK and are avirulent in animal models of disease. Resistance may be acquired less frequently by selecting variants which no longer recognize ACV or ACV triphosphate as substrates but which retain normal functions.

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3