P04405 (GLYG2_SOYBN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycinin G2

Cleaved into the following 2 chains:

1. Glycinin A2 subunit
2. Glycinin B1a subunit

Gene names

Name:

Gy2

Organism

Glycine max (Soybean) (Glycine hispida)

Taxonomic identifier

3847 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length	485 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Glycinin is the major seed storage protein of soybean.
Subunit structure	Hexamer; each subunit is composed of an acidic and a basic chain derived from a single precursor and linked by a disulfide bond. Ref.6 Ref.9
Sequence similarities	Belongs to the 11S seed storage protein (globulins) family.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Seed storage protein Storage protein
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular function	nutrient reservoir activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Ref.6
	Chain	19 – 296	278	Glycinin A2 subunit		PRO_0000032013
	Propeptide	297 – 300	4			PRO_0000032014
	Chain	301 – 480	180	Glycinin B1a subunit		PRO_0000032015
	Propeptide	481 – 485	5			PRO_0000032016
Amino acid modifications
	Disulfide bond	28 ↔ 61		By similarity
	Disulfide bond	104 ↔ 307		Interchain (between A2 and B1a chains) Ref.10
Natural variations
	Natural variant	103	1	G → D.
	Natural variant	318	1	N → T.
	Natural variant	331	1	I → V.
	Natural variant	413	1	K → R.
Experimental info
	Sequence conflict	39	1	D → G in CAA26575. Ref.5
	Sequence conflict	39	1	D → N AA sequence Ref.6
	Sequence conflict	61	1	C → S AA sequence Ref.6
	Sequence conflict	117	1	R → C AA sequence Ref.6
	Sequence conflict	343	1	W → S AA sequence Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P04405 [UniParc]. Last modified October 1, 1989. Version 2. Checksum: 78BB459837F77AD8	FASTA	485	54,391
	10 20 30 40 50 60 MAKLVLSLCF LLFSGCFALR EQAQQNECQI QKLNALKPDN RIESEGGFIE TWNPNNKPFQ 70 80 90 100 110 120 CAGVALSRCT LNRNALRRPS YTNGPQEIYI QQGNGIFGMI FPGCPSTYQE PQESQQRGRS 130 140 150 160 170 180 QRPQDRHQKV HRFREGDLIA VPTGVAWWMY NNEDTPVVAV SIIDTNSLEN QLDQMPRRFY 190 200 210 220 230 240 LAGNQEQEFL KYQQQQQGGS QSQKGKQQEE ENEGSNILSG FAPEFLKEAF GVNMQIVRNL 250 260 270 280 290 300 QGENEEEDSG AIVTVKGGLR VTAPAMRKPQ QEEDDDDEEE QPQCVETDKG CQRQSKRSRN 310 320 330 340 350 360 GIDETICTMR LRQNIGQNSS PDIYNPQAGS ITTATSLDFP ALWLLKLSAQ YGSLRKNAMF 370 380 390 400 410 420 VPHYTLNANS IIYALNGRAL VQVVNCNGER VFDGELQEGG VLIVPQNFAV AAKSQSDNFE 430 440 450 460 470 480 YVSFKTNDRP SIGNLAGANS LLNALPEEVI QHTFNLKSQQ ARQVKNNNPF SFLVPPQESQ RRAVA

References

[1]	"Characterization of the glycinin gene family in soybean." Nielsen N.C., Dickinson C.D., Cho T.-J., Thanh V.H., Scallon B.J., Fischer R.L., Sims T.L., Drews G.N., Goldberg R.B. Plant Cell 1:313-328(1989) [PubMed: 2485233] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. Dare. Tissue: Leaf.
[2]	"The glycinin Gy2 gene from soybean." Thanh V.H., Tumer N.E., Nielsen N.C. Nucleic Acids Res. 17:4387-4387(1989) [PubMed: 2740230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Dare. Tissue: Leaf.
[3]	"Polymorphism and expression of cDNAs encoding glycinin subunits." Utsumi S., Kim C.S., Kohno M., Kito M. Agric. Biol. Chem. 51:3267-3273(1987) Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. Shirotsurunoko.
[4]	"Complete nucleotide sequence of the gene encoding a glycinin A2B1a subunit precursor of soybean." Fukazawa C., Momma T., Higuchi W., Udaka K. Nucleic Acids Res. 15:8117-8117(1987) [PubMed: 3671077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"A complete cDNA coding for the sequence of glycinin A2B1a subunit precursor." Momma T., Negoro T., Udaka K., Fukazawa C. FEBS Lett. 188:117-122(1985) Cited for: NUCLEOTIDE SEQUENCE.
[6]	"The amino acid sequence of the A2B1a subunit of glycinin." Staswick P.E., Hermodson M.A., Nielsen N.C. J. Biol. Chem. 259:13424-13430(1984) [PubMed: 6541652] [Abstract] Cited for: PROTEIN SEQUENCE OF 19-296 AND 301-480 (A2 AND B1A SUBUNITS).
[7]	"Cloning and structural analysis of DNA encoding an A2B1a subunit of glycinin." Marco Y.A., Thanh V.H., Tumer N.E., Scallon B.J., Nielsen N.C. J. Biol. Chem. 259:13436-13441(1984) [PubMed: 6092376] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 262-485.
[8]	"The complete nucleotide sequence of soybean glycinin A2B1a gene spanning to another glycinin gene A1aB1b." Kitamura Y., Arahira M., Itoh Y., Fukazawa C. Nucleic Acids Res. 18:4245-4245(1990) [PubMed: 2377465] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-36.
[9]	"Limited proteolysis of beta-conglycinin and glycinin, the 7S and 11S storage globulins from soybean [Glycine max (L.) Merr.]. Structural and evolutionary implications." Shutov A.D., Kakhovskaya I.A., Bastrygina A.S., Bulmaga V.P., Horstmann C., Muntz K. Eur. J. Biochem. 241:221-228(1996) [PubMed: 8898910] [Abstract] Cited for: PROTEIN SEQUENCE OF 115-136 AND 202-224 (A2 SUBUNIT). Strain: cv. Kishinevskaya-16.
[10]	"Identification of the cystines which link the acidic and basic components of the glycinin subunits." Staswick P.E., Hermodson M.A., Nielsen N.C. J. Biol. Chem. 259:13431-13435(1984) [PubMed: 6541653] [Abstract] Cited for: DISULFIDE BOND.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X15122 Genomic DNA. Translation: CAA33216.1. D00216 mRNA. Translation: BAA00154.1. Y00398 Genomic DNA. Translation: CAA68460.1. X02806 mRNA. Translation: CAA26575.1. K02646 Genomic DNA. Translation: AAA33963.1. X53404 Genomic DNA. Translation: CAA37480.1.
PIR	FWSYG1. A91341. S11002.
RefSeq	NP_001235810.1. NM_001248881.1.
UniGene	Gma.1857.
3D structure databases
ProteinModelPortal	P04405.
SMR	P04405. Positions 26-479.
ModBase	Search...
Protein family/group databases
Allergome	1143. Gly m 6.0201. 5821. Gly m 6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	GLYMA03G32020.1; GLYMA03G32020.1; GLYMA03G32020. Gmax_FGT19685; Gmax_FGP19685; Gmax_FG19685.
GeneID	547900.
Phylogenomic databases
PhylomeDB	P04405.
Gene expression databases
Genevestigator	P04405.
Family and domain databases
InterPro	IPR022379. 11S_seedstore_CS. IPR006044. 11S_seedstore_pln. IPR006045. Cupin_1. IPR011051. Cupin_RmlC_type. IPR014710. RmlC-like_jellyroll. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 3 hits.
Pfam	PF00190. Cupin_1. 2 hits. [Graphical view]
PRINTS	PR00439. 11SGLOBULIN.
SMART	SM00835. Cupin_1. 2 hits. [Graphical view]
SUPFAM	SSF51182. RmlC_like_cupin. 2 hits.
PROSITE	PS00305. 11S_SEED_STORAGE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLYG2_SOYBN

Accession

Primary (citable) accession number: P04405
Secondary accession number(s): P04121, P04348, P04349

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 1987
Last sequence update:	October 1, 1989
Last modified:	December 14, 2011
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program