P04403 (2SS1_BEREX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2S sulfur-rich seed storage protein 1

Alternative name(s):
Allergen=Ber e 1

Cleaved into the following 2 chains:

1. 2S sulfur-rich seed storage protein small chain 1
   Alternative name(s):
   2S albumin 1 small subunit
2. 2S sulfur-rich seed storage protein large chain 1B
   Alternative name(s):
   2S albumin 1 large subunit

Gene names

Name:

BE2S1

Organism

Bertholletia excelsa (Brazil nut)

Taxonomic identifier

3645 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › Ericales › Lecythidaceae › Bertholletia

Protein attributes

Sequence length	146 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This is a 2S seed storage protein.
Subunit structure	The mature protein consists of a small and a large chain linked by disulfide bonds.
Allergenic properties	Causes an allergic reaction in human.
Sequence similarities	Belongs to the 2S seed storage albumins family.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Disease	Allergen
Domain	Signal
Molecular function	Seed storage protein Storage protein
PTM	Disulfide bond Pyrrolidone carboxylic acid
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Molecular function	nutrient reservoir activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 22

22

Potential

Propeptide

23 – 36

14

PRO_0000032105

Chain

37 – 64

28

2S sulfur-rich seed storage protein small chain 1

PRO_0000032106

Propeptide

65 – 69

5

PRO_0000032107

Chain

70 – 142

73

2S sulfur-rich seed storage protein large chain 1B

PRO_0000032108

Propeptide

143 – 146

4

PRO_0000032109

Amino acid modifications

Modified residue

37

1

Pyrrolidone carboxylic acid

Disulfide bond

40 ↔ 92

Interchain (between small and large chains) Ref.6

Disulfide bond

53 ↔ 81

Interchain (between small and large chains) Ref.6

Disulfide bond

82 ↔ 130

Ref.6

Disulfide bond

94 ↔ 137

Ref.6

Natural variations

Natural variant

91

1

S → E in variant 1A.

Experimental info

Sequence conflict

38 – 39

2

EE → QQ AA sequence Ref.5

Sequence conflict

102 – 103

2

MR → RM in BAA96554. Ref.4

Sequence conflict

107

1

E → K in BAA96554. Ref.4

Sequence conflict

122

1

L → M AA sequence Ref.5

Sequence conflict

126

1

I → L AA sequence Ref.5

Secondary structure

1

.

.

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.

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.

.

.

.

.

.

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.

.

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.

.

.

146

Helix Strand Turn

Details...

Helix

38 – 46

9

Helix

54 – 59

6

Helix

76 – 78

3

Helix

80 – 86

7

Turn

90 – 98

9

Helix

99 – 104

6

Turn

115 – 117

3

Helix

118 – 125

8

Turn

128 – 131

4

Beta strand

133 – 135

3

Sequences

	Sequence		Length	Mass (Da)
	P04403 [UniParc]. Last modified October 1, 1989. Version 2. Checksum: A7DF778FD766410D	FASTA	146	16,911
	10 20 30 40 50 60 MAKISVAAAA LLVLMALGHA TAFRATVTTT VVEEENQEEC REQMQRQQML SHCRMYMRQQ 70 80 90 100 110 120 MEESPYQTMP RRGMEPHMSE CCEQLEGMDE SCRCEGLRMM MMRMQQEEMQ PRGEQMRRMM 130 140 RLAENIPSRC NLSPMRCPMG GSIAGF

References

[1]	"Cloning and sequence analysis of a cDNA encoding a Brazil nut protein exceptionally rich in methionine." Altenbach S.B., Pearson K.W., Leung F.W., Sun S.S.M. Plant Mol. Biol. 8:239-250(1987) Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Bassuener R. Submitted (DEC-1990) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Isolation, characterization and expression of a gene coding for a 2S albumin from Bertholletia excelsa (Brazil nut)." Gander E.S., Holmstroem K.O., de Paiva G.R., de Castro L.A.B., Carneiro M., Grossi de Sa M.F. Plant Mol. Biol. 16:437-448(1991) [PubMed: 1840683] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Brazil nut 2S albumin was synthesized in a transgenic French bean seed with a promoter of the gene for canavalin, 7S globulin from Canavalia gladiata." Yamauchi D. Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"The amino-acid sequence of the 2S sulphur-rich proteins from seeds of Brazil nut (Bertholletia excelsa H.B.K.)." Ampe C., van Damme J., de Castro L.A.B., Sampaio M.J.A.M., van Montagu M., Vandekerckhove J. Eur. J. Biochem. 159:597-604(1986) [PubMed: 3758080] [Abstract] Cited for: PROTEIN SEQUENCE OF 37-64 AND 70-142.
[6]	"The disulphide mapping, folding and characterisation of recombinant Ber e 1, an allergenic protein, and SFA8, two sulphur-rich 2S plant albumins." Alcocer M.J., Murtagh G.J., Bailey K., Dumoulin M., Meseguer A.S., Parker M.J., Archer D.B. J. Mol. Biol. 324:165-175(2002) [PubMed: 12421566] [Abstract] Cited for: 3D-STRUCTURE MODELING, GLYCOSYLATION, DISULFIDE BONDS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M17146 mRNA. Translation: AAA33010.1.
X57027 Genomic DNA. Translation: CAA40343.1.
X57028 Genomic DNA. Translation: CAA40344.1.
X54490 Genomic DNA. Translation: CAA38362.1.
AB044391 Genomic DNA. Translation: BAA96554.1.

PIR

A25802.
S14946.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GYS	model	-	A	37-64	[»]
			B	69-142	[»]

ProteinModelPortal

P04403.

ModBase

Search...

Protein family/group databases

Allergome

3134. Ber e 1.0101.
88. Ber e 1.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR016140. Bifunc_inhib/LTP/seed_store.
IPR003612. LTP/seed_store/tryp_amyl_inhib.
IPR000617. Napin.
IPR013771. Trypsin/amylase_inhib.
[Graphical view]

Gene3D

G3DSA:1.10.120.10. Trypsin/amylase_inhib. 1 hit.

Pfam

PF00234. Tryp_alpha_amyl. 1 hit.
[Graphical view]

PRINTS

PR00496. NAPIN.

SMART

SM00499. AAI. 1 hit.
[Graphical view]

SUPFAM

SSF47699. Bifunc_inhib/LTP/seed_store. 1 hit.

ProtoNet

Search...

Entry information

Entry name

2SS1_BEREX

Accession

Primary (citable) accession number: P04403
Secondary accession number(s): P04402, Q9LRC2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 1987
Last sequence update:	October 1, 1989
Last modified:	September 21, 2011
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program