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Protein

2S sulfur-rich seed storage protein 1

Gene

BE2S1

Organism
Bertholletia excelsa (Brazil nut)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This is a 2S seed storage protein.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Seed storage protein, Storage protein

Names & Taxonomyi

Protein namesi
Recommended name:
2S sulfur-rich seed storage protein 1
Alternative name(s):
Allergen: Ber e 1
Cleaved into the following 2 chains:
Alternative name(s):
2S albumin 1 small subunit
Alternative name(s):
2S albumin 1 large subunit
Gene namesi
Name:BE2S1
OrganismiBertholletia excelsa (Brazil nut)
Taxonomic identifieri3645 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridsEricalesLecythidaceaeBertholletia

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3134. Ber e 1.0101.
88. Ber e 1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Propeptidei23 – 36141 PublicationPRO_0000032105Add
BLAST
Chaini37 – 64282S sulfur-rich seed storage protein small chain 1PRO_0000032106Add
BLAST
Propeptidei65 – 6951 PublicationPRO_0000032107
Chaini70 – 142732S sulfur-rich seed storage protein large chain 1BPRO_0000032108Add
BLAST
Propeptidei143 – 1464PRO_0000032109

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei37 – 371Pyrrolidone carboxylic acid
Disulfide bondi40 ↔ 92Interchain (between small and large chains)1 Publication
Disulfide bondi53 ↔ 81Interchain (between small and large chains)1 Publication
Disulfide bondi82 ↔ 1301 Publication
Disulfide bondi94 ↔ 1371 Publication

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Interactioni

Subunit structurei

The mature protein consists of a small and a large chain linked by disulfide bonds.

Structurei

Secondary structure

1
146
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi37 – 4610Combined sources
Helixi49 – 6214Combined sources
Turni63 – 664Combined sources
Beta strandi71 – 733Combined sources
Helixi76 – 8712Combined sources
Helixi90 – 10819Combined sources
Helixi114 – 13017Combined sources
Turni138 – 1414Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GYSmodel-A37-64[»]
B69-142[»]
2LVFNMR-A37-146[»]
ProteinModelPortaliP04403.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 2S seed storage albumins family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.10.120.10. 1 hit.
InterProiIPR016140. Bifunc_inhib/LTP/seed_store.
IPR000617. Napin.
IPR013771. Trypsin/amylase_inhib.
[Graphical view]
PfamiPF00234. Tryp_alpha_amyl. 1 hit.
[Graphical view]
PRINTSiPR00496. NAPIN.
SMARTiSM00499. AAI. 1 hit.
[Graphical view]
SUPFAMiSSF47699. SSF47699. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04403-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKISVAAAA LLVLMALGHA TAFRATVTTT VVEEENQEEC REQMQRQQML
60 70 80 90 100
SHCRMYMRQQ MEESPYQTMP RRGMEPHMSE CCEQLEGMDE SCRCEGLRMM
110 120 130 140
MMRMQQEEMQ PRGEQMRRMM RLAENIPSRC NLSPMRCPMG GSIAGF
Length:146
Mass (Da):16,911
Last modified:October 1, 1989 - v2
Checksum:iA7DF778FD766410D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti38 – 392EE → QQ AA sequence (PubMed:3758080).Curated
Sequence conflicti102 – 1032MR → RM in BAA96554 (Ref. 4) Curated
Sequence conflicti107 – 1071E → K in BAA96554 (Ref. 4) Curated
Sequence conflicti122 – 1221L → M AA sequence (PubMed:3758080).Curated
Sequence conflicti126 – 1261I → L AA sequence (PubMed:3758080).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti91 – 911S → E in variant 1A.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17146 mRNA. Translation: AAA33010.1.
X57027 Genomic DNA. Translation: CAA40343.1.
X57028 Genomic DNA. Translation: CAA40344.1.
X54490 Genomic DNA. Translation: CAA38362.1.
AB044391 Genomic DNA. Translation: BAA96554.1.
PIRiA25802.
S14946.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17146 mRNA. Translation: AAA33010.1.
X57027 Genomic DNA. Translation: CAA40343.1.
X57028 Genomic DNA. Translation: CAA40344.1.
X54490 Genomic DNA. Translation: CAA38362.1.
AB044391 Genomic DNA. Translation: BAA96554.1.
PIRiA25802.
S14946.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GYSmodel-A37-64[»]
B69-142[»]
2LVFNMR-A37-146[»]
ProteinModelPortaliP04403.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei3134. Ber e 1.0101.
88. Ber e 1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.10.120.10. 1 hit.
InterProiIPR016140. Bifunc_inhib/LTP/seed_store.
IPR000617. Napin.
IPR013771. Trypsin/amylase_inhib.
[Graphical view]
PfamiPF00234. Tryp_alpha_amyl. 1 hit.
[Graphical view]
PRINTSiPR00496. NAPIN.
SMARTiSM00499. AAI. 1 hit.
[Graphical view]
SUPFAMiSSF47699. SSF47699. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Cloning and sequence analysis of a cDNA encoding a Brazil nut protein exceptionally rich in methionine."
    Altenbach S.B., Pearson K.W., Leung F.W., Sun S.S.M.
    Plant Mol. Biol. 8:239-250(1987)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Bassuener R.
    Submitted (DEC-1990) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Isolation, characterization and expression of a gene coding for a 2S albumin from Bertholletia excelsa (Brazil nut)."
    Gander E.S., Holmstroem K.O., de Paiva G.R., de Castro L.A.B., Carneiro M., Grossi de Sa M.F.
    Plant Mol. Biol. 16:437-448(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Brazil nut 2S albumin was synthesized in a transgenic French bean seed with a promoter of the gene for canavalin, 7S globulin from Canavalia gladiata."
    Yamauchi D.
    Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "The amino-acid sequence of the 2S sulphur-rich proteins from seeds of Brazil nut (Bertholletia excelsa H.B.K.)."
    Ampe C., van Damme J., de Castro L.A.B., Sampaio M.J.A.M., van Montagu M., Vandekerckhove J.
    Eur. J. Biochem. 159:597-604(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 37-64 AND 70-142.
  6. "The disulphide mapping, folding and characterisation of recombinant Ber e 1, an allergenic protein, and SFA8, two sulphur-rich 2S plant albumins."
    Alcocer M.J., Murtagh G.J., Bailey K., Dumoulin M., Meseguer A.S., Parker M.J., Archer D.B.
    J. Mol. Biol. 324:165-175(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING, GLYCOSYLATION, DISULFIDE BONDS.

Entry informationi

Entry namei2SS1_BEREX
AccessioniPrimary (citable) accession number: P04403
Secondary accession number(s): P04402, Q9LRC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: October 1, 1989
Last modified: November 26, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.