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P04397 (GAL10_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 138. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional protein GAL10

Including the following 2 domains:

  1. UDP-glucose 4-epimerase
    EC=5.1.3.2
    Alternative name(s):
    Galactowaldenase
  2. Aldose 1-epimerase
    EC=5.1.3.3
    Alternative name(s):
    Galactose mutarotase
Gene names
Name:GAL10
Ordered Locus Names:YBR019C
ORF Names:YBR0301
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length699 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mutarotase converts alpha-aldose to the beta-anomer. It is active on D-glucose, L-arabinose, D-xylose, D-galactose, maltose and lactose By similarity.

Catalytic activity

UDP-alpha-D-glucose = UDP-alpha-D-galactose.

Alpha-D-glucose = beta-D-glucose.

Cofactor

NAD.

Pathway

Carbohydrate metabolism; galactose metabolism.

Carbohydrate metabolism; hexose metabolism.

Induction

By galactose.

Sequence similarities

In the N-terminal section; belongs to the NAD(P)-dependent epimerase/dehydratase family.

In the C-terminal section; belongs to the aldose epimerase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 699699Bifunctional protein GAL10
PRO_0000197442

Regions

Nucleotide binding13 – 4432NAD Potential
Region1 – 357357Galactowaldenase
Region358 – 699342Mutarotase

Sites

Active site5371For mutarotase activity Potential

Amino acid modifications

Modified residue5621Phosphoserine Ref.8

Experimental info

Sequence conflict302 – 3065DLPYK → WSSVR no nucleotide entry Ref.5
Sequence conflict4641F → L no nucleotide entry Ref.5
Sequence conflict479 – 4802KD → NY no nucleotide entry Ref.5
Sequence conflict4981P → Q no nucleotide entry Ref.5
Sequence conflict5181M → I no nucleotide entry Ref.5
Sequence conflict6671G → C no nucleotide entry Ref.5
Sequence conflict694 – 6952IV → TL no nucleotide entry Ref.5

Secondary structure

.............................................................................................................. 699
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P04397 [UniParc].

Last modified October 1, 1994. Version 2.
Checksum: 8354BAFF65D06934

FASTA69978,195
        10         20         30         40         50         60 
MTAQLQSEST SKIVLVTGGA GYIGSHTVVE LIENGYDCVV ADNLSNSTYD SVARLEVLTK 

        70         80         90        100        110        120 
HHIPFYEVDL CDRKGLEKVF KEYKIDSVIH FAGLKAVGES TQIPLRYYHN NILGTVVLLE 

       130        140        150        160        170        180 
LMQQYNVSKF VFSSSATVYG DATRFPNMIP IPEECPLGPT NPYGHTKYAI ENILNDLYNS 

       190        200        210        220        230        240 
DKKSWKFAIL RYFNPIGAHP SGLIGEDPLG IPNNLLPYMA QVAVGRREKL YIFGDDYDSR 

       250        260        270        280        290        300 
DGTPIRDYIH VVDLAKGHIA ALQYLEAYNE NEGLCREWNL GSGKGSTVFE VYHAFCKASG 

       310        320        330        340        350        360 
IDLPYKVTGR RAGDVLNLTA KPDRAKRELK WQTELQVEDS CKDLWKWTTE NPFGYQLRGV 

       370        380        390        400        410        420 
EARFSAEDMR YDARFVTIGA GTRFQATFAN LGASIVDLKV NGQSVVLGYE NEEGYLNPDS 

       430        440        450        460        470        480 
AYIGATIGRY ANRISKGKFS LCNKDYQLTV NNGVNANHSS IGSFHRKRFL GPIIQNPSKD 

       490        500        510        520        530        540 
VFTAEYMLID NEKDTEFPGD LLVTIQYTVN VAQKSLEMVY KGKLTAGEAT PINLTNHSYF 

       550        560        570        580        590        600 
NLNKPYGDTI EGTEIMVRSK KSVDVDKNMI PTGNIVDREI ATFNSTKPTV LGPKNPQFDC 

       610        620        630        640        650        660 
CFVVDENAKP SQINTLNNEL TLIVKAFHPD SNITLEVLST EPTYQFYTGD FLSAGYEARQ 

       670        680        690 
GFAIEPGRYI DAINQENWKD CVTLKNGETY GSKIVYRFS 

« Hide

References

« Hide 'large scale' references
[1]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"The complete sequence of a 33 kb fragment on the right arm of chromosome II from Saccharomyces cerevisiae reveals 16 open reading frames, including ten new open reading frames, five previously identified genes and a homologue of the SCO1 gene."
Smits P.H.M., de Haan M., Maat C., Grivell L.A.
Yeast 10:S75-S80(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-500.
Strain: ATCC 204508 / S288c.
[4]"Sequence and functional analysis of a 7.2 kb fragment of Saccharomyces cerevisiae chromosome II including GAL7 and GAL10 and a new essential open reading frame."
Schaaff-Gerstenschlaeger I., Schindwolf T., Lehnert W., Rose M., Zimmermann F.K.
Yeast 11:79-83(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 134-699.
Strain: ATCC 204508 / S288c.
[5]"Sequence of the Saccharomyces GAL region and its transcription in vivo."
Citron B.A., Donelson J.E.
J. Bacteriol. 158:269-278(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-47 AND 302-699.
Strain: Carlsbergensis.
[6]"Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae."
Johnston M., Davis R.W.
Mol. Cell. Biol. 4:1440-1448(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-46.
[7]"Primary structure of the Saccharomyces cerevisiae GAL7 gene."
Tajima M., Nogi Y., Fukasawa T.
Yeast 1:67-77(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 630-699.
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-562, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z35888 Genomic DNA. Translation: CAA84961.1.
X81324 Genomic DNA. Translation: CAA57106.1.
K02115 Genomic DNA. Translation: AAA34620.1.
M12348 Genomic DNA. No translation available.
K01609 Genomic DNA. Translation: AAA34630.1.
BK006936 Genomic DNA. Translation: DAA07141.1.
PIRXEBYUG. S45875.
RefSeqNP_009575.1. NM_001178367.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1Z45X-ray1.85A1-699[»]
ProteinModelPortalP04397.
SMRP04397. Positions 11-699.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32722. 53 interactions.
DIPDIP-4891N.
MINTMINT-526001.
STRING4932.YBR019C.

Proteomic databases

PaxDbP04397.
PeptideAtlasP04397.
PRIDEP04397.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBR019C; YBR019C; YBR019C.
GeneID852307.
KEGGsce:YBR019C.

Organism-specific databases

CYGDYBR019c.
SGDS000000223. GAL10.

Phylogenomic databases

eggNOGCOG2017.
GeneTreeENSGT00530000063128.
HOGENOMHOG000247896.
KOK01784.
K01785.
OMADLWKWTT.
OrthoDBEOG77T1F2.

Enzyme and pathway databases

BioCycYEAST:YBR019C-MONOMER.
UniPathwayUPA00214.
UPA00242.

Gene expression databases

GenevestigatorP04397.

Family and domain databases

Gene3D2.70.98.10. 1 hit.
3.40.50.720. 1 hit.
InterProIPR018052. Ald1_epimerase_CS.
IPR008183. Aldose_1/G6P_1-epimerase.
IPR025308. Epimerase_C.
IPR001509. Epimerase_deHydtase.
IPR011013. Gal_mutarotase_SF_dom.
IPR005886. GalE.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR10366:SF39. PTHR10366:SF39. 1 hit.
PfamPF01263. Aldose_epim. 1 hit.
PF01370. Epimerase. 1 hit.
PF13950. Epimerase_Csub. 1 hit.
[Graphical view]
SUPFAMSSF74650. SSF74650. 1 hit.
TIGRFAMsTIGR01179. galE. 1 hit.
PROSITEPS00545. ALDOSE_1_EPIMERASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP04397.
NextBio970981.
PROP04397.

Entry information

Entry nameGAL10_YEAST
AccessionPrimary (citable) accession number: P04397
Secondary accession number(s): D6VQ21
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: October 1, 1994
Last modified: June 11, 2014
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways