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P04397

- GAL10_YEAST

UniProt

P04397 - GAL10_YEAST

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Protein

Bifunctional protein GAL10

Gene

GAL10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Mutarotase converts alpha-aldose to the beta-anomer. It is active on D-glucose, L-arabinose, D-xylose, D-galactose, maltose and lactose (By similarity).By similarity

Catalytic activityi

UDP-alpha-D-glucose = UDP-alpha-D-galactose.
Alpha-D-glucose = beta-D-glucose.PROSITE-ProRule annotation

Cofactori

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei537 – 5371For mutarotase activitySequence Analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi13 – 4432NADSequence AnalysisAdd
BLAST

GO - Molecular functioni

  1. aldose 1-epimerase activity Source: SGD
  2. carbohydrate binding Source: InterPro
  3. coenzyme binding Source: InterPro
  4. UDP-glucose 4-epimerase activity Source: SGD

GO - Biological processi

  1. galactose catabolic process via UDP-galactose Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Carbohydrate metabolism, Galactose metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciYEAST:YBR019C-MONOMER.
ReactomeiREACT_232344. Galactose catabolism.
UniPathwayiUPA00214.
UPA00242.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein GAL10
Including the following 2 domains:
UDP-glucose 4-epimerase (EC:5.1.3.2)
Alternative name(s):
Galactowaldenase
Aldose 1-epimerase (EC:5.1.3.3)
Alternative name(s):
Galactose mutarotase
Gene namesi
Name:GAL10
Ordered Locus Names:YBR019C
ORF Names:YBR0301
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR019c.
SGDiS000000223. GAL10.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 699699Bifunctional protein GAL10PRO_0000197442Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei562 – 5621Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP04397.
PeptideAtlasiP04397.
PRIDEiP04397.

Expressioni

Inductioni

By galactose.

Gene expression databases

GenevestigatoriP04397.

Interactioni

Protein-protein interaction databases

BioGridi32722. 53 interactions.
DIPiDIP-4891N.
MINTiMINT-526001.
STRINGi4932.YBR019C.

Structurei

Secondary structure

1
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi13 – 175Combined sources
Turni18 – 203Combined sources
Helixi22 – 3312Combined sources
Beta strandi37 – 426Combined sources
Helixi50 – 5910Combined sources
Beta strandi65 – 673Combined sources
Helixi73 – 8210Combined sources
Beta strandi87 – 904Combined sources
Helixi97 – 1026Combined sources
Helixi104 – 12522Combined sources
Beta strandi129 – 1357Combined sources
Helixi136 – 1394Combined sources
Helixi142 – 1443Combined sources
Helixi162 – 18019Combined sources
Beta strandi186 – 1927Combined sources
Beta strandi194 – 1963Combined sources
Beta strandi209 – 2113Combined sources
Helixi215 – 2239Combined sources
Beta strandi226 – 2283Combined sources
Beta strandi248 – 2503Combined sources
Helixi251 – 26717Combined sources
Beta strandi274 – 2818Combined sources
Helixi288 – 29912Combined sources
Helixi323 – 3286Combined sources
Helixi337 – 35014Combined sources
Beta strandi360 – 3678Combined sources
Beta strandi373 – 3797Combined sources
Beta strandi382 – 39110Combined sources
Beta strandi395 – 4006Combined sources
Helixi412 – 4154Combined sources
Beta strandi432 – 4343Combined sources
Helixi435 – 4373Combined sources
Beta strandi438 – 4414Combined sources
Beta strandi444 – 4474Combined sources
Beta strandi455 – 4573Combined sources
Helixi460 – 4623Combined sources
Helixi464 – 4663Combined sources
Beta strandi470 – 4789Combined sources
Beta strandi481 – 49010Combined sources
Helixi492 – 4943Combined sources
Beta strandi495 – 51016Combined sources
Turni511 – 5144Combined sources
Beta strandi515 – 53117Combined sources
Turni542 – 5454Combined sources
Beta strandi548 – 5503Combined sources
Beta strandi553 – 5586Combined sources
Beta strandi561 – 5655Combined sources
Beta strandi571 – 5777Combined sources
Beta strandi585 – 5873Combined sources
Beta strandi589 – 5913Combined sources
Beta strandi599 – 6046Combined sources
Beta strandi621 – 6277Combined sources
Turni629 – 6313Combined sources
Beta strandi634 – 64714Combined sources
Beta strandi662 – 6687Combined sources
Helixi672 – 6743Combined sources
Turni676 – 6783Combined sources
Helixi679 – 6824Combined sources
Beta strandi683 – 6853Combined sources
Beta strandi689 – 69911Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Z45X-ray1.85A1-699[»]
ProteinModelPortaliP04397.
SMRiP04397. Positions 11-699.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04397.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 357357GalactowaldenaseAdd
BLAST
Regioni358 – 699342MutarotaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the NAD(P)-dependent epimerase/dehydratase family.Curated
In the C-terminal section; belongs to the aldose epimerase family.Curated

Phylogenomic databases

eggNOGiCOG2017.
GeneTreeiENSGT00530000063128.
HOGENOMiHOG000247896.
InParanoidiP04397.
KOiK01784.
K01785.
OMAiDLWKWTT.
OrthoDBiEOG77T1F2.

Family and domain databases

Gene3Di2.70.98.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR018052. Ald1_epimerase_CS.
IPR008183. Aldose_1/G6P_1-epimerase.
IPR025308. Epimerase_C.
IPR001509. Epimerase_deHydtase_N.
IPR011013. Gal_mutarotase_SF_dom.
IPR005886. GalE.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10366:SF39. PTHR10366:SF39. 1 hit.
PfamiPF01263. Aldose_epim. 1 hit.
PF01370. Epimerase. 1 hit.
PF13950. Epimerase_Csub. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.
TIGRFAMsiTIGR01179. galE. 1 hit.
PROSITEiPS00545. ALDOSE_1_EPIMERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04397-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTAQLQSEST SKIVLVTGGA GYIGSHTVVE LIENGYDCVV ADNLSNSTYD
60 70 80 90 100
SVARLEVLTK HHIPFYEVDL CDRKGLEKVF KEYKIDSVIH FAGLKAVGES
110 120 130 140 150
TQIPLRYYHN NILGTVVLLE LMQQYNVSKF VFSSSATVYG DATRFPNMIP
160 170 180 190 200
IPEECPLGPT NPYGHTKYAI ENILNDLYNS DKKSWKFAIL RYFNPIGAHP
210 220 230 240 250
SGLIGEDPLG IPNNLLPYMA QVAVGRREKL YIFGDDYDSR DGTPIRDYIH
260 270 280 290 300
VVDLAKGHIA ALQYLEAYNE NEGLCREWNL GSGKGSTVFE VYHAFCKASG
310 320 330 340 350
IDLPYKVTGR RAGDVLNLTA KPDRAKRELK WQTELQVEDS CKDLWKWTTE
360 370 380 390 400
NPFGYQLRGV EARFSAEDMR YDARFVTIGA GTRFQATFAN LGASIVDLKV
410 420 430 440 450
NGQSVVLGYE NEEGYLNPDS AYIGATIGRY ANRISKGKFS LCNKDYQLTV
460 470 480 490 500
NNGVNANHSS IGSFHRKRFL GPIIQNPSKD VFTAEYMLID NEKDTEFPGD
510 520 530 540 550
LLVTIQYTVN VAQKSLEMVY KGKLTAGEAT PINLTNHSYF NLNKPYGDTI
560 570 580 590 600
EGTEIMVRSK KSVDVDKNMI PTGNIVDREI ATFNSTKPTV LGPKNPQFDC
610 620 630 640 650
CFVVDENAKP SQINTLNNEL TLIVKAFHPD SNITLEVLST EPTYQFYTGD
660 670 680 690
FLSAGYEARQ GFAIEPGRYI DAINQENWKD CVTLKNGETY GSKIVYRFS
Length:699
Mass (Da):78,195
Last modified:October 1, 1994 - v2
Checksum:i8354BAFF65D06934
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti302 – 3065DLPYK → WSSVR no nucleotide entry (PubMed:6715281)Curated
Sequence conflicti464 – 4641F → L no nucleotide entry (PubMed:6715281)Curated
Sequence conflicti479 – 4802KD → NY no nucleotide entry (PubMed:6715281)Curated
Sequence conflicti498 – 4981P → Q no nucleotide entry (PubMed:6715281)Curated
Sequence conflicti518 – 5181M → I no nucleotide entry (PubMed:6715281)Curated
Sequence conflicti667 – 6671G → C no nucleotide entry (PubMed:6715281)Curated
Sequence conflicti694 – 6952IV → TL no nucleotide entry (PubMed:6715281)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35888 Genomic DNA. Translation: CAA84961.1.
X81324 Genomic DNA. Translation: CAA57106.1.
K02115 Genomic DNA. Translation: AAA34620.1.
M12348 Genomic DNA. No translation available.
K01609 Genomic DNA. Translation: AAA34630.1.
BK006936 Genomic DNA. Translation: DAA07141.1.
PIRiS45875. XEBYUG.
RefSeqiNP_009575.1. NM_001178367.1.

Genome annotation databases

EnsemblFungiiYBR019C; YBR019C; YBR019C.
GeneIDi852307.
KEGGisce:YBR019C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35888 Genomic DNA. Translation: CAA84961.1 .
X81324 Genomic DNA. Translation: CAA57106.1 .
K02115 Genomic DNA. Translation: AAA34620.1 .
M12348 Genomic DNA. No translation available.
K01609 Genomic DNA. Translation: AAA34630.1 .
BK006936 Genomic DNA. Translation: DAA07141.1 .
PIRi S45875. XEBYUG.
RefSeqi NP_009575.1. NM_001178367.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1Z45 X-ray 1.85 A 1-699 [» ]
ProteinModelPortali P04397.
SMRi P04397. Positions 11-699.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32722. 53 interactions.
DIPi DIP-4891N.
MINTi MINT-526001.
STRINGi 4932.YBR019C.

Proteomic databases

PaxDbi P04397.
PeptideAtlasi P04397.
PRIDEi P04397.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR019C ; YBR019C ; YBR019C .
GeneIDi 852307.
KEGGi sce:YBR019C.

Organism-specific databases

CYGDi YBR019c.
SGDi S000000223. GAL10.

Phylogenomic databases

eggNOGi COG2017.
GeneTreei ENSGT00530000063128.
HOGENOMi HOG000247896.
InParanoidi P04397.
KOi K01784.
K01785.
OMAi DLWKWTT.
OrthoDBi EOG77T1F2.

Enzyme and pathway databases

UniPathwayi UPA00214 .
UPA00242 .
BioCyci YEAST:YBR019C-MONOMER.
Reactomei REACT_232344. Galactose catabolism.

Miscellaneous databases

EvolutionaryTracei P04397.
NextBioi 970981.
PROi P04397.

Gene expression databases

Genevestigatori P04397.

Family and domain databases

Gene3Di 2.70.98.10. 1 hit.
3.40.50.720. 1 hit.
InterProi IPR018052. Ald1_epimerase_CS.
IPR008183. Aldose_1/G6P_1-epimerase.
IPR025308. Epimerase_C.
IPR001509. Epimerase_deHydtase_N.
IPR011013. Gal_mutarotase_SF_dom.
IPR005886. GalE.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR10366:SF39. PTHR10366:SF39. 1 hit.
Pfami PF01263. Aldose_epim. 1 hit.
PF01370. Epimerase. 1 hit.
PF13950. Epimerase_Csub. 1 hit.
[Graphical view ]
SUPFAMi SSF74650. SSF74650. 1 hit.
TIGRFAMsi TIGR01179. galE. 1 hit.
PROSITEi PS00545. ALDOSE_1_EPIMERASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "The complete sequence of a 33 kb fragment on the right arm of chromosome II from Saccharomyces cerevisiae reveals 16 open reading frames, including ten new open reading frames, five previously identified genes and a homologue of the SCO1 gene."
    Smits P.H.M., de Haan M., Maat C., Grivell L.A.
    Yeast 10:S75-S80(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-500.
    Strain: ATCC 204508 / S288c.
  4. "Sequence and functional analysis of a 7.2 kb fragment of Saccharomyces cerevisiae chromosome II including GAL7 and GAL10 and a new essential open reading frame."
    Schaaff-Gerstenschlaeger I., Schindwolf T., Lehnert W., Rose M., Zimmermann F.K.
    Yeast 11:79-83(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 134-699.
    Strain: ATCC 204508 / S288c.
  5. "Sequence of the Saccharomyces GAL region and its transcription in vivo."
    Citron B.A., Donelson J.E.
    J. Bacteriol. 158:269-278(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-47 AND 302-699.
    Strain: Carlsbergensis.
  6. "Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae."
    Johnston M., Davis R.W.
    Mol. Cell. Biol. 4:1440-1448(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-46.
  7. "Primary structure of the Saccharomyces cerevisiae GAL7 gene."
    Tajima M., Nogi Y., Fukasawa T.
    Yeast 1:67-77(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 630-699.
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-562, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGAL10_YEAST
AccessioniPrimary (citable) accession number: P04397
Secondary accession number(s): D6VQ21
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: October 1, 1994
Last modified: November 26, 2014
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3