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Protein

DNA-3-methyladenine glycosylase 2

Gene

alkA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2-methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions.

Catalytic activityi

Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei218Determinant for substrate specificity and/or activity1
Active sitei238Proton acceptor1

GO - Molecular functioni

GO - Biological processi

  • base-excision repair Source: EcoliWiki
  • base-excision repair, AP site formation Source: GO_Central
  • cellular response to DNA damage stimulus Source: EcoliWiki
  • DNA dealkylation involved in DNA repair Source: EcoliWiki
  • DNA repair Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Enzyme and pathway databases

BioCyciEcoCyc:EG11222-MONOMER.
ECOL316407:JW2053-MONOMER.
MetaCyc:EG11222-MONOMER.
BRENDAi3.2.2.21. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-3-methyladenine glycosylase 2 (EC:3.2.2.21)
Alternative name(s):
3-methyladenine-DNA glycosylase II, inducible
Short name:
TAG II
DNA-3-methyladenine glycosidase II
DNA-3-methyladenine glycosylase II
Gene namesi
Name:alkA
Synonyms:aidA
Ordered Locus Names:b2068, JW2053
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11222. alkA.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
  • protein-DNA complex Source: GO_Central
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi124Q → A: Methylmethane sulfonate-resistant. 1 Publication1
Mutagenesisi218W → A: No catalytic activity, methylmethane sulfonate-sensitive. 1 Publication1
Mutagenesisi237D → N: More than 30% catalytic activity, methylmethane sulfonate-resistant. 1 Publication1
Mutagenesisi238D → N: No catalytic activity, methylmethane sulfonate-sensitive. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001948781 – 282DNA-3-methyladenine glycosylase 2Add BLAST282

Proteomic databases

PaxDbiP04395.
PRIDEiP04395.

Expressioni

Inductioni

Up-regulated by methylated Ada in response to the exposure to alkylating agents.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4259681. 55 interactors.
DIPiDIP-9084N.
IntActiP04395. 19 interactors.
MINTiMINT-1227621.
STRINGi511145.b2068.

Structurei

Secondary structure

1282
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 5Combined sources4
Helixi12 – 22Combined sources11
Turni25 – 27Combined sources3
Beta strandi28 – 30Combined sources3
Beta strandi35 – 41Combined sources7
Beta strandi44 – 53Combined sources10
Turni54 – 57Combined sources4
Beta strandi58 – 63Combined sources6
Helixi65 – 70Combined sources6
Helixi71 – 82Combined sources12
Turni83 – 85Combined sources3
Helixi88 – 95Combined sources8
Helixi96 – 99Combined sources4
Helixi113 – 122Combined sources10
Turni123 – 125Combined sources3
Helixi128 – 142Combined sources15
Beta strandi149 – 153Combined sources5
Helixi158 – 162Combined sources5
Helixi166 – 171Combined sources6
Helixi176 – 190Combined sources15
Helixi202 – 209Combined sources8
Helixi217 – 227Combined sources11
Helixi239 – 244Combined sources6
Beta strandi245 – 247Combined sources3
Helixi250 – 257Combined sources8
Helixi258 – 260Combined sources3
Helixi264 – 272Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DIZX-ray2.50A/B1-282[»]
1MPGX-ray1.80A/B1-282[»]
1PVSX-ray2.40A/B1-282[»]
3CVSX-ray2.40A/B/C/D1-282[»]
3CVTX-ray2.50A/B/C/D1-282[»]
3CW7X-ray2.30A/B/C/D1-282[»]
3CWAX-ray2.40A/B/C/D1-282[»]
3CWSX-ray2.30A/B/C/D1-282[»]
3CWTX-ray2.30A/B/C/D1-282[»]
3CWUX-ray2.80A/B/C/D1-282[»]
3D4VX-ray2.90A/B/C/D1-282[»]
3OGDX-ray2.80A2-282[»]
3OH6X-ray2.89A2-282[»]
3OH9X-ray2.80A2-282[»]
ProteinModelPortaliP04395.
SMRiP04395.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04395.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105D03. Bacteria.
COG0122. LUCA.
HOGENOMiHOG000221908.
InParanoidiP04395.
KOiK01247.
OMAiLHIWYTD.
PhylomeDBiP04395.

Family and domain databases

CDDicd00056. ENDO3c. 1 hit.
Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
3.30.310.20. 1 hit.
InterProiIPR010316. AlkA_N.
IPR000035. Alkylbase_DNA_glycsylse_CS.
IPR011257. DNA_glycosylase.
IPR003265. HhH-GPD_domain.
IPR023170. HTH_base_excis_C.
[Graphical view]
PfamiPF06029. AlkA_N. 1 hit.
PF00730. HhH-GPD. 1 hit.
[Graphical view]
SMARTiSM01009. AlkA_N. 1 hit.
SM00478. ENDO3c. 1 hit.
[Graphical view]
SUPFAMiSSF48150. SSF48150. 1 hit.
PROSITEiPS00516. ALKYLBASE_DNA_GLYCOS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04395-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYTLNWQPPY DWSWMLGFLA ARAVSSVETV ADSYYARSLA VGEYRGVVTA
60 70 80 90 100
IPDIARHTLH INLSAGLEPV AAECLAKMSR LFDLQCNPQI VNGALGRLGA
110 120 130 140 150
ARPGLRLPGC VDAFEQGVRA ILGQLVSVAM AAKLTARVAQ LYGERLDDFP
160 170 180 190 200
EYICFPTPQR LAAADPQALK ALGMPLKRAE ALIHLANAAL EGTLPMTIPG
210 220 230 240 250
DVEQAMKTLQ TFPGIGRWTA NYFALRGWQA KDVFLPDDYL IKQRFPGMTP
260 270 280
AQIRRYAERW KPWRSYALLH IWYTEGWQPD EA
Length:282
Mass (Da):31,393
Last modified:March 20, 1987 - v1
Checksum:iB66BB5E23019899C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02498 Genomic DNA. Translation: AAA23430.1.
U00096 Genomic DNA. Translation: AAC75129.1.
AP009048 Genomic DNA. Translation: BAA15926.1.
M13827 Genomic DNA. No translation available.
PIRiA00904. DGECMA.
RefSeqiNP_416572.1. NC_000913.3.
WP_000288420.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75129; AAC75129; b2068.
BAA15926; BAA15926; BAA15926.
GeneIDi947371.
KEGGiecj:JW2053.
eco:b2068.
PATRICi32119469. VBIEscCol129921_2145.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02498 Genomic DNA. Translation: AAA23430.1.
U00096 Genomic DNA. Translation: AAC75129.1.
AP009048 Genomic DNA. Translation: BAA15926.1.
M13827 Genomic DNA. No translation available.
PIRiA00904. DGECMA.
RefSeqiNP_416572.1. NC_000913.3.
WP_000288420.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DIZX-ray2.50A/B1-282[»]
1MPGX-ray1.80A/B1-282[»]
1PVSX-ray2.40A/B1-282[»]
3CVSX-ray2.40A/B/C/D1-282[»]
3CVTX-ray2.50A/B/C/D1-282[»]
3CW7X-ray2.30A/B/C/D1-282[»]
3CWAX-ray2.40A/B/C/D1-282[»]
3CWSX-ray2.30A/B/C/D1-282[»]
3CWTX-ray2.30A/B/C/D1-282[»]
3CWUX-ray2.80A/B/C/D1-282[»]
3D4VX-ray2.90A/B/C/D1-282[»]
3OGDX-ray2.80A2-282[»]
3OH6X-ray2.89A2-282[»]
3OH9X-ray2.80A2-282[»]
ProteinModelPortaliP04395.
SMRiP04395.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259681. 55 interactors.
DIPiDIP-9084N.
IntActiP04395. 19 interactors.
MINTiMINT-1227621.
STRINGi511145.b2068.

Proteomic databases

PaxDbiP04395.
PRIDEiP04395.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75129; AAC75129; b2068.
BAA15926; BAA15926; BAA15926.
GeneIDi947371.
KEGGiecj:JW2053.
eco:b2068.
PATRICi32119469. VBIEscCol129921_2145.

Organism-specific databases

EchoBASEiEB1204.
EcoGeneiEG11222. alkA.

Phylogenomic databases

eggNOGiENOG4105D03. Bacteria.
COG0122. LUCA.
HOGENOMiHOG000221908.
InParanoidiP04395.
KOiK01247.
OMAiLHIWYTD.
PhylomeDBiP04395.

Enzyme and pathway databases

BioCyciEcoCyc:EG11222-MONOMER.
ECOL316407:JW2053-MONOMER.
MetaCyc:EG11222-MONOMER.
BRENDAi3.2.2.21. 2026.

Miscellaneous databases

EvolutionaryTraceiP04395.
PROiP04395.

Family and domain databases

CDDicd00056. ENDO3c. 1 hit.
Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
3.30.310.20. 1 hit.
InterProiIPR010316. AlkA_N.
IPR000035. Alkylbase_DNA_glycsylse_CS.
IPR011257. DNA_glycosylase.
IPR003265. HhH-GPD_domain.
IPR023170. HTH_base_excis_C.
[Graphical view]
PfamiPF06029. AlkA_N. 1 hit.
PF00730. HhH-GPD. 1 hit.
[Graphical view]
SMARTiSM01009. AlkA_N. 1 hit.
SM00478. ENDO3c. 1 hit.
[Graphical view]
SUPFAMiSSF48150. SSF48150. 1 hit.
PROSITEiPS00516. ALKYLBASE_DNA_GLYCOS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei3MG2_ECOLI
AccessioniPrimary (citable) accession number: P04395
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: March 20, 1987
Last modified: November 30, 2016
This is version 151 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.