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P04394 (NDUV2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 135. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

EC=1.6.5.3
EC=1.6.99.3
Alternative name(s):
NADH dehydrogenase subunit II
NADH-ubiquinone oxidoreductase 24 kDa subunit
Gene names
Name:NDUFV2
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length249 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Catalytic activity

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).

NADH + acceptor = NAD+ + reduced acceptor.

Cofactor

Binds 1 2Fe-2S cluster Potential.

Subunit structure

Complex I is composed of 45 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme.

Subcellular location

Mitochondrion inner membrane.

Sequence similarities

Belongs to the complex I 24 kDa subunit family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3232Mitochondrion Ref.4
Chain33 – 249217NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
PRO_0000020002

Sites

Metal binding1351Iron-sulfur (2Fe-2S) Potential
Metal binding1401Iron-sulfur (2Fe-2S) Potential
Metal binding1761Iron-sulfur (2Fe-2S) Potential
Metal binding1801Iron-sulfur (2Fe-2S) Potential

Amino acid modifications

Modified residue611N6-acetyllysine By similarity
Modified residue1931Phosphotyrosine; by SRC By similarity

Experimental info

Sequence conflict89 – 979QNGWLPISA → SSGTSYPDVLKBZZZPPGGA AIILW AA sequence Ref.4

Sequences

Sequence LengthMass (Da)Tools
P04394 [UniParc].

Last modified February 1, 1991. Version 3.
Checksum: 8F7E8259F08EBF65

FASTA24927,308
        10         20         30         40         50         60 
MFLSAALRAR AAGLAAHWGK HIRNLHKTAV QNGAGGALFV HRDTPENNPE TPFDFTPENY 

        70         80         90        100        110        120 
KRIEAIVKNY PEGHKAAAVL PVLDLAQRQN GWLPISAMNK VAEILQVPPM RVYEVATFYT 

       130        140        150        160        170        180 
MYNRKPVGKY HIQVCTTTPC MLRNSDSILE AIQKKLGIKV GETTPDKLFT LIEVECLGAC 

       190        200        210        220        230        240 
VNAPMVQIND NYYEDLTPKD IEEIIDELKA GKIPKPGPRS GRFSCEPAGG LTSLTEPPKG 


PGFGVQAGL 

« Hide

References

« Hide 'large scale' references
[1]"Mitochondrial NADH-ubiquinone reductase: complementary DNA sequences of import precursors of the bovine and human 24-kDa subunit."
Pilkington S.J., Walker J.E.
Biochemistry 28:3257-3264(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Crossbred X Angus.
Tissue: Ileum.
[3]"cDNA of the 24 kDa subunit of the bovine respiratory chain NADH dehydrogenase: high sequence conservation in mammals and tissue-specific and growth-dependent expression."
Chomyn A., Tsai Lai S.S.-A.
Curr. Genet. 16:117-125(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 38-249.
Tissue: Brain.
[4]"The primary structure of subunit II of NADH dehydrogenase from bovine-heart mitochondria."
von Bahr-Lindstroem H., Galante Y.M., Persson M., Joernvall H.
Eur. J. Biochem. 134:145-150(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 33-249.
Tissue: Heart.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X14724 mRNA. Translation: CAA32848.1.
BC102401 mRNA. Translation: AAI02402.1.
M22539 mRNA. Translation: AAA87358.1.
PIRB30113.
RefSeqNP_776990.1. NM_174565.3.
UniGeneBt.88596.

3D structure databases

ProteinModelPortalP04394.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP04394. 3 interactions.
STRING9913.ENSBTAP00000052906.

Protein family/group databases

TCDB3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbP04394.
PRIDEP04394.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000006405; ENSBTAP00000052906; ENSBTAG00000004871.
GeneID282290.
KEGGbta:282290.

Organism-specific databases

CTD4729.

Phylogenomic databases

eggNOGCOG1905.
GeneTreeENSGT00390000017580.
HOGENOMHOG000257748.
HOVERGENHBG029601.
InParanoidP04394.
KOK03943.
OMAVHRDTPY.
OrthoDBEOG73JKW9.
TreeFamTF300004.

Family and domain databases

Gene3D3.40.30.10. 1 hit.
InterProIPR002023. NuoE-like.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERPTHR10371. PTHR10371. 1 hit.
SUPFAMSSF52833. SSF52833. 1 hit.
TIGRFAMsTIGR01958. nuoE_fam. 1 hit.
PROSITEPS01099. COMPLEX1_24K. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20806092.

Entry information

Entry nameNDUV2_BOVIN
AccessionPrimary (citable) accession number: P04394
Secondary accession number(s): Q3T0G9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: February 1, 1991
Last modified: June 11, 2014
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families