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Protein

NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

Gene

NDUFV2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

[2Fe-2S] clusterCuratedNote: Binds 1 [2Fe-2S] cluster.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi135 – 1351Iron-sulfur (2Fe-2S)Sequence Analysis
Metal bindingi140 – 1401Iron-sulfur (2Fe-2S)Sequence Analysis
Metal bindingi176 – 1761Iron-sulfur (2Fe-2S)Sequence Analysis
Metal bindingi180 – 1801Iron-sulfur (2Fe-2S)Sequence Analysis

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. electron carrier activity Source: GO_Central
  3. metal ion binding Source: UniProtKB-KW
  4. NADH dehydrogenase (ubiquinone) activity Source: GO_Central

GO - Biological processi

  1. cardiac muscle tissue development Source: Ensembl
  2. mitochondrial electron transport, NADH to ubiquinone Source: GO_Central
  3. nervous system development Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

ReactomeiREACT_223064. Respiratory electron transport.

Protein family/group databases

TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
NADH dehydrogenase subunit II
NADH-ubiquinone oxidoreductase 24 kDa subunit
Gene namesi
Name:NDUFV2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 24

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial respiratory chain complex I Source: GO_Central
  2. mitochondrion Source: AgBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3232Mitochondrion1 PublicationAdd
BLAST
Chaini33 – 249217NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrialPRO_0000020002Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei61 – 611N6-acetyllysineBy similarity
Modified residuei193 – 1931Phosphotyrosine; by SRCBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP04394.
PRIDEiP04394.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme.

Protein-protein interaction databases

IntActiP04394. 3 interactions.
STRINGi9913.ENSBTAP00000052906.

Structurei

3D structure databases

ProteinModelPortaliP04394.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 24 kDa subunit family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1905.
GeneTreeiENSGT00390000017580.
HOGENOMiHOG000257748.
HOVERGENiHBG029601.
InParanoidiP04394.
KOiK03943.
OMAiVHRDTPY.
OrthoDBiEOG73JKW9.
TreeFamiTF300004.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR002023. NuoE-like.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR10371. PTHR10371. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR01958. nuoE_fam. 1 hit.
PROSITEiPS01099. COMPLEX1_24K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04394-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLSAALRAR AAGLAAHWGK HIRNLHKTAV QNGAGGALFV HRDTPENNPE
60 70 80 90 100
TPFDFTPENY KRIEAIVKNY PEGHKAAAVL PVLDLAQRQN GWLPISAMNK
110 120 130 140 150
VAEILQVPPM RVYEVATFYT MYNRKPVGKY HIQVCTTTPC MLRNSDSILE
160 170 180 190 200
AIQKKLGIKV GETTPDKLFT LIEVECLGAC VNAPMVQIND NYYEDLTPKD
210 220 230 240
IEEIIDELKA GKIPKPGPRS GRFSCEPAGG LTSLTEPPKG PGFGVQAGL
Length:249
Mass (Da):27,308
Last modified:February 1, 1991 - v3
Checksum:i8F7E8259F08EBF65
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti89 – 979QNGWLPISA → SSGTSYPDVLKBZZZPPGGA AIILW AA sequence (PubMed:6861757).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14724 mRNA. Translation: CAA32848.1.
BC102401 mRNA. Translation: AAI02402.1.
M22539 mRNA. Translation: AAA87358.1.
PIRiB30113.
RefSeqiNP_776990.1. NM_174565.3.
UniGeneiBt.88596.

Genome annotation databases

EnsembliENSBTAT00000006405; ENSBTAP00000052906; ENSBTAG00000004871.
GeneIDi282290.
KEGGibta:282290.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14724 mRNA. Translation: CAA32848.1.
BC102401 mRNA. Translation: AAI02402.1.
M22539 mRNA. Translation: AAA87358.1.
PIRiB30113.
RefSeqiNP_776990.1. NM_174565.3.
UniGeneiBt.88596.

3D structure databases

ProteinModelPortaliP04394.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP04394. 3 interactions.
STRINGi9913.ENSBTAP00000052906.

Protein family/group databases

TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbiP04394.
PRIDEiP04394.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000006405; ENSBTAP00000052906; ENSBTAG00000004871.
GeneIDi282290.
KEGGibta:282290.

Organism-specific databases

CTDi4729.

Phylogenomic databases

eggNOGiCOG1905.
GeneTreeiENSGT00390000017580.
HOGENOMiHOG000257748.
HOVERGENiHBG029601.
InParanoidiP04394.
KOiK03943.
OMAiVHRDTPY.
OrthoDBiEOG73JKW9.
TreeFamiTF300004.

Enzyme and pathway databases

ReactomeiREACT_223064. Respiratory electron transport.

Miscellaneous databases

NextBioi20806092.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR002023. NuoE-like.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR10371. PTHR10371. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR01958. nuoE_fam. 1 hit.
PROSITEiPS01099. COMPLEX1_24K. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mitochondrial NADH-ubiquinone reductase: complementary DNA sequences of import precursors of the bovine and human 24-kDa subunit."
    Pilkington S.J., Walker J.E.
    Biochemistry 28:3257-3264(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Ileum.
  3. "cDNA of the 24 kDa subunit of the bovine respiratory chain NADH dehydrogenase: high sequence conservation in mammals and tissue-specific and growth-dependent expression."
    Chomyn A., Tsai Lai S.S.-A.
    Curr. Genet. 16:117-125(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 38-249.
    Tissue: Brain.
  4. "The primary structure of subunit II of NADH dehydrogenase from bovine-heart mitochondria."
    von Bahr-Lindstroem H., Galante Y.M., Persson M., Joernvall H.
    Eur. J. Biochem. 134:145-150(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 33-249.
    Tissue: Heart.

Entry informationi

Entry nameiNDUV2_BOVIN
AccessioniPrimary (citable) accession number: P04394
Secondary accession number(s): Q3T0G9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: February 1, 1991
Last modified: March 4, 2015
This is version 141 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.