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Protein

NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

Gene

NDUFV2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

[2Fe-2S] clusterCuratedNote: Binds 1 [2Fe-2S] cluster.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi135Iron-sulfur (2Fe-2S)Sequence analysis1
Metal bindingi140Iron-sulfur (2Fe-2S)Sequence analysis1
Metal bindingi176Iron-sulfur (2Fe-2S)Sequence analysis1
Metal bindingi180Iron-sulfur (2Fe-2S)Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

ReactomeiR-BTA-611105. Respiratory electron transport.
R-BTA-6799198. Complex I biogenesis.

Protein family/group databases

TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
NADH dehydrogenase subunit II
NADH-ubiquinone oxidoreductase 24 kDa subunit
Gene namesi
Name:NDUFV2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 24

Subcellular locationi

GO - Cellular componenti

  • mitochondrial respiratory chain complex I Source: UniProtKB
  • mitochondrion Source: AgBase
  • myelin sheath Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 32Mitochondrion1 PublicationAdd BLAST32
ChainiPRO_000002000233 – 249NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrialAdd BLAST217

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei61N6-acetyllysineBy similarity1
Modified residuei193Phosphotyrosine; by SRCBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP04394.
PeptideAtlasiP04394.
PRIDEiP04394.

Expressioni

Gene expression databases

BgeeiENSBTAG00000004871.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme.2 Publications

Protein-protein interaction databases

IntActiP04394. 3 interactors.
STRINGi9913.ENSBTAP00000052906.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5LC5electron microscopy4.35E1-249[»]
5LDWelectron microscopy4.27E33-249[»]
5LDXelectron microscopy5.60E33-249[»]
ProteinModelPortaliP04394.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 24 kDa subunit family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3196. Eukaryota.
COG1905. LUCA.
GeneTreeiENSGT00390000017580.
HOGENOMiHOG000257748.
HOVERGENiHBG029601.
InParanoidiP04394.
KOiK03943.
OMAiHKQAATI.
OrthoDBiEOG091G0GRD.
TreeFamiTF300004.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR002023. NuoE-like.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PIRSFiPIRSF000216. NADH_DH_24kDa. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR01958. nuoE_fam. 1 hit.
PROSITEiPS01099. COMPLEX1_24K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04394-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLSAALRAR AAGLAAHWGK HIRNLHKTAV QNGAGGALFV HRDTPENNPE
60 70 80 90 100
TPFDFTPENY KRIEAIVKNY PEGHKAAAVL PVLDLAQRQN GWLPISAMNK
110 120 130 140 150
VAEILQVPPM RVYEVATFYT MYNRKPVGKY HIQVCTTTPC MLRNSDSILE
160 170 180 190 200
AIQKKLGIKV GETTPDKLFT LIEVECLGAC VNAPMVQIND NYYEDLTPKD
210 220 230 240
IEEIIDELKA GKIPKPGPRS GRFSCEPAGG LTSLTEPPKG PGFGVQAGL
Length:249
Mass (Da):27,308
Last modified:February 1, 1991 - v3
Checksum:i8F7E8259F08EBF65
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti89 – 97QNGWLPISA → SSGTSYPDVLKBZZZPPGGA AIILW AA sequence (PubMed:6861757).Curated9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14724 mRNA. Translation: CAA32848.1.
BC102401 mRNA. Translation: AAI02402.1.
M22539 mRNA. Translation: AAA87358.1.
PIRiB30113.
RefSeqiNP_776990.1. NM_174565.3.
UniGeneiBt.88596.

Genome annotation databases

EnsembliENSBTAT00000006405; ENSBTAP00000052906; ENSBTAG00000004871.
GeneIDi282290.
KEGGibta:282290.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14724 mRNA. Translation: CAA32848.1.
BC102401 mRNA. Translation: AAI02402.1.
M22539 mRNA. Translation: AAA87358.1.
PIRiB30113.
RefSeqiNP_776990.1. NM_174565.3.
UniGeneiBt.88596.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5LC5electron microscopy4.35E1-249[»]
5LDWelectron microscopy4.27E33-249[»]
5LDXelectron microscopy5.60E33-249[»]
ProteinModelPortaliP04394.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP04394. 3 interactors.
STRINGi9913.ENSBTAP00000052906.

Protein family/group databases

TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbiP04394.
PeptideAtlasiP04394.
PRIDEiP04394.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000006405; ENSBTAP00000052906; ENSBTAG00000004871.
GeneIDi282290.
KEGGibta:282290.

Organism-specific databases

CTDi4729.

Phylogenomic databases

eggNOGiKOG3196. Eukaryota.
COG1905. LUCA.
GeneTreeiENSGT00390000017580.
HOGENOMiHOG000257748.
HOVERGENiHBG029601.
InParanoidiP04394.
KOiK03943.
OMAiHKQAATI.
OrthoDBiEOG091G0GRD.
TreeFamiTF300004.

Enzyme and pathway databases

ReactomeiR-BTA-611105. Respiratory electron transport.
R-BTA-6799198. Complex I biogenesis.

Gene expression databases

BgeeiENSBTAG00000004871.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR002023. NuoE-like.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PIRSFiPIRSF000216. NADH_DH_24kDa. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR01958. nuoE_fam. 1 hit.
PROSITEiPS01099. COMPLEX1_24K. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNDUV2_BOVIN
AccessioniPrimary (citable) accession number: P04394
Secondary accession number(s): Q3T0G9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: February 1, 1991
Last modified: November 2, 2016
This is version 153 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.