NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial precursor

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P04394 (NDUV2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 125. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
EC=1.6.5.3
EC=1.6.99.3

Alternative name(s):
NADH dehydrogenase subunit II
NADH-ubiquinone oxidoreductase 24 kDa subunit

Gene names

Name:

NDUFV2

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Catalytic activity	NADH + ubiquinone = NAD⁺ + ubiquinol. NADH + acceptor = NAD⁺ + reduced acceptor.
Cofactor	Binds 1 2Fe-2S cluster Potential.
Subunit structure	Complex I is composed of 45 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme.
Subcellular location	Mitochondrion inner membrane.
Sequence similarities	Belongs to the complex I 24 kDa subunit family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cardiac muscle tissue development Inferred from electronic annotation. Source: Compara mitochondrial electron transport, NADH to ubiquinone Inferred from sequence or structural similarity. Source: RefGenome nervous system development Inferred from electronic annotation. Source: Compara
Cellular_component	mitochondrial respiratory chain complex I Inferred from sequence or structural similarity. Source: RefGenome
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from sequence or structural similarity. Source: RefGenome metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 32

Mitochondrion Ref.4

Chain

33 – 249

217

NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

PRO_0000020002

Sites

Metal binding

135

Iron-sulfur (2Fe-2S) Potential

Metal binding

140

Iron-sulfur (2Fe-2S) Potential

Metal binding

176

Iron-sulfur (2Fe-2S) Potential

Metal binding

180

Iron-sulfur (2Fe-2S) Potential

Experimental info

Sequence conflict

89 – 97

QNGWLPISA → SSGTSYPDVLKBZZZPPGGA AIILW AA sequence Ref.4

Sequences

Sequence

Length

Mass (Da)

Tools

P04394 [UniParc].

Last modified February 1, 1991. Version 3.
Checksum: 8F7E8259F08EBF65
Show »

FASTA

249

27,308

        10         20         30         40         50         60 
MFLSAALRAR AAGLAAHWGK HIRNLHKTAV QNGAGGALFV HRDTPENNPE TPFDFTPENY 

        70         80         90        100        110        120 
KRIEAIVKNY PEGHKAAAVL PVLDLAQRQN GWLPISAMNK VAEILQVPPM RVYEVATFYT 

       130        140        150        160        170        180 
MYNRKPVGKY HIQVCTTTPC MLRNSDSILE AIQKKLGIKV GETTPDKLFT LIEVECLGAC 

       190        200        210        220        230        240 
VNAPMVQIND NYYEDLTPKD IEEIIDELKA GKIPKPGPRS GRFSCEPAGG LTSLTEPPKG 


PGFGVQAGL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mitochondrial NADH-ubiquinone reductase: complementary DNA sequences of import precursors of the bovine and human 24-kDa subunit." Pilkington S.J., Walker J.E. Biochemistry 28:3257-3264(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.
[3]	"cDNA of the 24 kDa subunit of the bovine respiratory chain NADH dehydrogenase: high sequence conservation in mammals and tissue-specific and growth-dependent expression." Chomyn A., Tsai Lai S.S.-A. Curr. Genet. 16:117-125(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 38-249. Tissue: Brain.
[4]	"The primary structure of subunit II of NADH dehydrogenase from bovine-heart mitochondria." von Bahr-Lindstroem H., Galante Y.M., Persson M., Joernvall H. Eur. J. Biochem. 134:145-150(1983) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 33-249. Tissue: Heart.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X14724 mRNA. Translation: CAA32848.1. BC102401 mRNA. Translation: AAI02402.1. M22539 mRNA. Translation: AAA87358.1.
IPI	IPI00692522.
PIR	B30113.
RefSeq	NP_776990.1. NM_174565.3.
UniGene	Bt.88596.
3D structure databases
ProteinModelPortal	P04394.
ModBase	Search...
Protein-protein interaction databases
IntAct	P04394. 3 interactions.
STRING	9913.ENSBTAP00000052906.
Protein family/group databases
TCDB	3.D.1.6.1. H+ or Na+-translocating NADH dehydrogenase (NDH) family.
Proteomic databases
PaxDb	P04394.
PRIDE	P04394.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000006405; ENSBTAP00000052906; ENSBTAG00000004871.
GeneID	282290.
KEGG	bta:282290.
Organism-specific databases
CTD	4729.
Phylogenomic databases
eggNOG	COG1905.
GeneTree	ENSGT00390000017580.
HOGENOM	HOG000257748.
HOVERGEN	HBG029601.
InParanoid	P04394.
KO	K03943.
OMA	KPGPRNG.
OrthoDB	EOG4QFWDW.
Gene expression databases
ArrayExpress	P04394.
Family and domain databases
Gene3D	3.40.30.10. 1 hit.
InterPro	IPR002023. 2Fe-2S_Ferredox. IPR012336. Thioredoxin-like_fold. [Graphical view]
PANTHER	PTHR10371. PTHR10371. 1 hit.
Pfam	PF01257. Complex1_24kDa. 1 hit. [Graphical view]
SUPFAM	SSF52833. Thiordxn-like_fd. 1 hit.
TIGRFAMs	TIGR01958. nuoE_fam. 1 hit.
PROSITE	PS01099. COMPLEX1_24K. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20806092.

Entry information

Entry name

NDUV2_BOVIN

Accession

Primary (citable) accession number: P04394
Secondary accession number(s): Q3T0G9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 1987
Last sequence update:	February 1, 1991
Last modified:	April 3, 2013
This is version 125 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents