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P04394

- NDUV2_BOVIN

UniProt

P04394 - NDUV2_BOVIN

Protein

NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

Gene

NDUFV2

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 3 (01 Feb 1991)
      Previous versions | rss
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    Functioni

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

    Catalytic activityi

    NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
    NADH + acceptor = NAD+ + reduced acceptor.

    Cofactori

    Binds 1 2Fe-2S cluster.Curated

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi135 – 1351Iron-sulfur (2Fe-2S)Sequence Analysis
    Metal bindingi140 – 1401Iron-sulfur (2Fe-2S)Sequence Analysis
    Metal bindingi176 – 1761Iron-sulfur (2Fe-2S)Sequence Analysis
    Metal bindingi180 – 1801Iron-sulfur (2Fe-2S)Sequence Analysis

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. electron carrier activity Source: RefGenome
    3. metal ion binding Source: UniProtKB-KW
    4. NADH dehydrogenase (ubiquinone) activity Source: RefGenome

    GO - Biological processi

    1. cardiac muscle tissue development Source: Ensembl
    2. mitochondrial electron transport, NADH to ubiquinone Source: RefGenome
    3. nervous system development Source: Ensembl

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    2Fe-2S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

    Enzyme and pathway databases

    ReactomeiREACT_223064. Respiratory electron transport.

    Protein family/group databases

    TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
    Alternative name(s):
    NADH dehydrogenase subunit II
    NADH-ubiquinone oxidoreductase 24 kDa subunit
    Gene namesi
    Name:NDUFV2
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 24

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial respiratory chain complex I Source: RefGenome
    2. mitochondrion Source: AgBase

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3232Mitochondrion1 PublicationAdd
    BLAST
    Chaini33 – 249217NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrialPRO_0000020002Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei61 – 611N6-acetyllysineBy similarity
    Modified residuei193 – 1931Phosphotyrosine; by SRCBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiP04394.
    PRIDEiP04394.

    Interactioni

    Subunit structurei

    Complex I is composed of 45 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme.

    Protein-protein interaction databases

    IntActiP04394. 3 interactions.
    STRINGi9913.ENSBTAP00000052906.

    Structurei

    3D structure databases

    ProteinModelPortaliP04394.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the complex I 24 kDa subunit family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1905.
    GeneTreeiENSGT00390000017580.
    HOGENOMiHOG000257748.
    HOVERGENiHBG029601.
    InParanoidiP04394.
    KOiK03943.
    OMAiVHRDTPY.
    OrthoDBiEOG73JKW9.
    TreeFamiTF300004.

    Family and domain databases

    Gene3Di3.40.30.10. 1 hit.
    InterProiIPR002023. NuoE-like.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PANTHERiPTHR10371. PTHR10371. 1 hit.
    SUPFAMiSSF52833. SSF52833. 1 hit.
    TIGRFAMsiTIGR01958. nuoE_fam. 1 hit.
    PROSITEiPS01099. COMPLEX1_24K. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P04394-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFLSAALRAR AAGLAAHWGK HIRNLHKTAV QNGAGGALFV HRDTPENNPE    50
    TPFDFTPENY KRIEAIVKNY PEGHKAAAVL PVLDLAQRQN GWLPISAMNK 100
    VAEILQVPPM RVYEVATFYT MYNRKPVGKY HIQVCTTTPC MLRNSDSILE 150
    AIQKKLGIKV GETTPDKLFT LIEVECLGAC VNAPMVQIND NYYEDLTPKD 200
    IEEIIDELKA GKIPKPGPRS GRFSCEPAGG LTSLTEPPKG PGFGVQAGL 249
    Length:249
    Mass (Da):27,308
    Last modified:February 1, 1991 - v3
    Checksum:i8F7E8259F08EBF65
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti89 – 979QNGWLPISA → SSGTSYPDVLKBZZZPPGGA AIILW AA sequence (PubMed:6861757)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14724 mRNA. Translation: CAA32848.1.
    BC102401 mRNA. Translation: AAI02402.1.
    M22539 mRNA. Translation: AAA87358.1.
    PIRiB30113.
    RefSeqiNP_776990.1. NM_174565.3.
    UniGeneiBt.88596.

    Genome annotation databases

    EnsembliENSBTAT00000006405; ENSBTAP00000052906; ENSBTAG00000004871.
    GeneIDi282290.
    KEGGibta:282290.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14724 mRNA. Translation: CAA32848.1 .
    BC102401 mRNA. Translation: AAI02402.1 .
    M22539 mRNA. Translation: AAA87358.1 .
    PIRi B30113.
    RefSeqi NP_776990.1. NM_174565.3.
    UniGenei Bt.88596.

    3D structure databases

    ProteinModelPortali P04394.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P04394. 3 interactions.
    STRINGi 9913.ENSBTAP00000052906.

    Protein family/group databases

    TCDBi 3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Proteomic databases

    PaxDbi P04394.
    PRIDEi P04394.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000006405 ; ENSBTAP00000052906 ; ENSBTAG00000004871 .
    GeneIDi 282290.
    KEGGi bta:282290.

    Organism-specific databases

    CTDi 4729.

    Phylogenomic databases

    eggNOGi COG1905.
    GeneTreei ENSGT00390000017580.
    HOGENOMi HOG000257748.
    HOVERGENi HBG029601.
    InParanoidi P04394.
    KOi K03943.
    OMAi VHRDTPY.
    OrthoDBi EOG73JKW9.
    TreeFami TF300004.

    Enzyme and pathway databases

    Reactomei REACT_223064. Respiratory electron transport.

    Miscellaneous databases

    NextBioi 20806092.

    Family and domain databases

    Gene3Di 3.40.30.10. 1 hit.
    InterProi IPR002023. NuoE-like.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    PANTHERi PTHR10371. PTHR10371. 1 hit.
    SUPFAMi SSF52833. SSF52833. 1 hit.
    TIGRFAMsi TIGR01958. nuoE_fam. 1 hit.
    PROSITEi PS01099. COMPLEX1_24K. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mitochondrial NADH-ubiquinone reductase: complementary DNA sequences of import precursors of the bovine and human 24-kDa subunit."
      Pilkington S.J., Walker J.E.
      Biochemistry 28:3257-3264(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Crossbred X Angus.
      Tissue: Ileum.
    3. "cDNA of the 24 kDa subunit of the bovine respiratory chain NADH dehydrogenase: high sequence conservation in mammals and tissue-specific and growth-dependent expression."
      Chomyn A., Tsai Lai S.S.-A.
      Curr. Genet. 16:117-125(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 38-249.
      Tissue: Brain.
    4. "The primary structure of subunit II of NADH dehydrogenase from bovine-heart mitochondria."
      von Bahr-Lindstroem H., Galante Y.M., Persson M., Joernvall H.
      Eur. J. Biochem. 134:145-150(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 33-249.
      Tissue: Heart.

    Entry informationi

    Entry nameiNDUV2_BOVIN
    AccessioniPrimary (citable) accession number: P04394
    Secondary accession number(s): Q3T0G9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 20, 1987
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 137 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3