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Protein

Modification methylase EcoRV

Gene

ecoRVM

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GATATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the EcoRV endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3397. M.EcoRV.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase EcoRV (EC:2.1.1.72)
Short name:
M.EcoRV
Alternative name(s):
Adenine-specific methyltransferase EcoRV
Gene namesi
Name:ecoRVM
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 298298Modification methylase EcoRVPRO_0000087971Add
BLAST

Proteomic databases

PRIDEiP04393.

Structurei

3D structure databases

ProteinModelPortaliP04393.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Phylogenomic databases

KOiK06223.

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProiIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04393-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDKVFVPPI KSQGIKTKLV PCIKRIVPKN FNGVWVEPFM GTGVVAFNVA
60 70 80 90 100
PKDALLCDTN PHLISFYNAL KNKDITGDLV KDFLYREGEK LLLSNGEYYY
110 120 130 140 150
EVRERFNNYK EPLDFLFLNR SCFNGMIRFN SKGGFNVPFC KKPNRFAQAY
160 170 180 190 200
ITKISNQVDR ISEIISKGNY TFLCQSFEKT IGMVNRDDVV YCDPPYIGRH
210 220 230 240 250
VDYFNSWGER DERLLFETLS SLNATFITST WHHNDYRENK YVRDLWSSFR
260 270 280 290
ILTKEHFYHV GASEKNRSPM VEALITNIAK DIIDHIEKSS GDILVIEE
Length:298
Mass (Da):34,639
Last modified:March 20, 1987 - v1
Checksum:i5FF2263E733574D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00530 Genomic DNA. Translation: CAA25209.1.
M19941 Genomic DNA. Translation: AAA24614.1.
PIRiA00557. XYECR5.
RefSeqiNP_863581.1. NC_005019.1.
WP_011117660.1. NC_005019.1.

Genome annotation databases

GeneIDi1446622.
KEGGipg:1446622.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00530 Genomic DNA. Translation: CAA25209.1.
M19941 Genomic DNA. Translation: AAA24614.1.
PIRiA00557. XYECR5.
RefSeqiNP_863581.1. NC_005019.1.
WP_011117660.1. NC_005019.1.

3D structure databases

ProteinModelPortaliP04393.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3397. M.EcoRV.

Proteomic databases

PRIDEiP04393.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1446622.
KEGGipg:1446622.

Phylogenomic databases

KOiK06223.

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProiIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization of the genes coding for the Eco RV restriction and modification system of Escherichia coli."
    Bougueleret L., Schwarzstein M., Tsugita A., Zabeau M.
    Nucleic Acids Res. 12:3659-3676(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The EcoRV restriction-modification system: genes, enzymes, synthetic substrates."
    Kraev A.S., Kravets A.N., Chernov B.K., Skryabin K.G., Baev A.A.
    Mol. Biol. (Mosk.) 19:236-242(1985)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-45.

Entry informationi

Entry nameiMTE5_ECOLX
AccessioniPrimary (citable) accession number: P04393
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: March 20, 1987
Last modified: April 1, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.