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P04393 (MTE5_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase EcoRV

Short name=M.EcoRV
EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase EcoRV
Gene names
Name:ecoRVM
OrganismEscherichia coli
Taxonomic identifier562 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length298 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GATATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the EcoRV endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 298298Modification methylase EcoRV
PRO_0000087971

Sequences

Sequence LengthMass (Da)Tools
P04393 [UniParc].

Last modified March 20, 1987. Version 1.
Checksum: 5FF2263E733574D5

FASTA29834,639
        10         20         30         40         50         60 
MKDKVFVPPI KSQGIKTKLV PCIKRIVPKN FNGVWVEPFM GTGVVAFNVA PKDALLCDTN 

        70         80         90        100        110        120 
PHLISFYNAL KNKDITGDLV KDFLYREGEK LLLSNGEYYY EVRERFNNYK EPLDFLFLNR 

       130        140        150        160        170        180 
SCFNGMIRFN SKGGFNVPFC KKPNRFAQAY ITKISNQVDR ISEIISKGNY TFLCQSFEKT 

       190        200        210        220        230        240 
IGMVNRDDVV YCDPPYIGRH VDYFNSWGER DERLLFETLS SLNATFITST WHHNDYRENK 

       250        260        270        280        290 
YVRDLWSSFR ILTKEHFYHV GASEKNRSPM VEALITNIAK DIIDHIEKSS GDILVIEE 

« Hide

References

[1]"Characterization of the genes coding for the Eco RV restriction and modification system of Escherichia coli."
Bougueleret L., Schwarzstein M., Tsugita A., Zabeau M.
Nucleic Acids Res. 12:3659-3676(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The EcoRV restriction-modification system: genes, enzymes, synthetic substrates."
Kraev A.S., Kravets A.N., Chernov B.K., Skryabin K.G., Baev A.A.
Mol. Biol. (Mosk.) 19:236-242(1985)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-45.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X00530 Genomic DNA. Translation: CAA25209.1.
M19941 Genomic DNA. Translation: AAA24614.1.
PIRXYECR5. A00557.
RefSeqNP_863581.1. NC_005019.1.

3D structure databases

ProteinModelPortalP04393.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3397. M.EcoRV.

Proteomic databases

PRIDEP04393.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1446622.

Family and domain databases

Gene3D1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSPR00505. D12N6MTFRASE.
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00571. dam. 1 hit.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTE5_ECOLX
AccessionPrimary (citable) accession number: P04393
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: March 20, 1987
Last modified: June 11, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries