P04393 (MTE5_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase EcoRV
Short name=M.EcoRV
EC=2.1.1.72

Alternative name(s):
Adenine-specific methyltransferase EcoRV

Gene names

Name:

ecoRVM

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GATATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the EcoRV endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 298	298	Modification methylase EcoRV		PRO_0000087971

Sequences

	Sequence		Length	Mass (Da)
	P04393 [UniParc]. Last modified March 20, 1987. Version 1. Checksum: 5FF2263E733574D5	FASTA	298	34,639
	10 20 30 40 50 60 MKDKVFVPPI KSQGIKTKLV PCIKRIVPKN FNGVWVEPFM GTGVVAFNVA PKDALLCDTN 70 80 90 100 110 120 PHLISFYNAL KNKDITGDLV KDFLYREGEK LLLSNGEYYY EVRERFNNYK EPLDFLFLNR 130 140 150 160 170 180 SCFNGMIRFN SKGGFNVPFC KKPNRFAQAY ITKISNQVDR ISEIISKGNY TFLCQSFEKT 190 200 210 220 230 240 IGMVNRDDVV YCDPPYIGRH VDYFNSWGER DERLLFETLS SLNATFITST WHHNDYRENK 250 260 270 280 290 YVRDLWSSFR ILTKEHFYHV GASEKNRSPM VEALITNIAK DIIDHIEKSS GDILVIEE

References

[1]	"Characterization of the genes coding for the Eco RV restriction and modification system of Escherichia coli." Bougueleret L., Schwarzstein M., Tsugita A., Zabeau M. Nucleic Acids Res. 12:3659-3676(1984) [PubMed: 6328432] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The EcoRV restriction-modification system: genes, enzymes, synthetic substrates." Kraev A.S., Kravets A.N., Chernov B.K., Skryabin K.G., Baev A.A. Mol. Biol. (Mosk.) 19:236-242(1985) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-45.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X00530 Genomic DNA. Translation: CAA25209.1. M19941 Genomic DNA. Translation: AAA24614.1.
PIR	XYECR5. A00557.
RefSeq	NP_863581.1. NC_005019.1.
3D structure databases
ProteinModelPortal	P04393.
ModBase	Search...
Protein family/group databases
REBASE	3397. M.EcoRV.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1446622.
Phylogenomic databases
ProtClustDB	CLSK918371.
Family and domain databases
InterPro	IPR023095. Ade_MeTrfase_dom_2. IPR002052. DNA_methylase_N6_adenine_CS. IPR012263. M_m6A_EcoRV. IPR012327. MeTrfase_D12. [Graphical view]
Gene3D	G3DSA:1.10.1020.10. Ade_MeTrfase_dom_2. 1 hit.
Pfam	PF02086. MethyltransfD12. 1 hit. [Graphical view]
PIRSF	PIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTS	PR00505. D12N6MTFRASE.
TIGRFAMs	TIGR00571. Dam. 1 hit.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTE5_ECOLX

Accession

Primary (citable) accession number: P04393

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 1987
Last sequence update:	March 20, 1987
Last modified:	September 21, 2011
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program