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P04393

- MTE5_ECOLX

UniProt

P04393 - MTE5_ECOLX

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Protein
Modification methylase EcoRV
Gene
ecoRVM
Organism
Escherichia coli
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GATATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the EcoRV endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3397. M.EcoRV.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase EcoRV (EC:2.1.1.72)
Short name:
M.EcoRV
Alternative name(s):
Adenine-specific methyltransferase EcoRV
Gene namesi
Name:ecoRVM
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 298298Modification methylase EcoRV
PRO_0000087971Add
BLAST

Proteomic databases

PRIDEiP04393.

Structurei

3D structure databases

ProteinModelPortaliP04393.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProiIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04393-1 [UniParc]FASTAAdd to Basket

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MKDKVFVPPI KSQGIKTKLV PCIKRIVPKN FNGVWVEPFM GTGVVAFNVA    50
PKDALLCDTN PHLISFYNAL KNKDITGDLV KDFLYREGEK LLLSNGEYYY 100
EVRERFNNYK EPLDFLFLNR SCFNGMIRFN SKGGFNVPFC KKPNRFAQAY 150
ITKISNQVDR ISEIISKGNY TFLCQSFEKT IGMVNRDDVV YCDPPYIGRH 200
VDYFNSWGER DERLLFETLS SLNATFITST WHHNDYRENK YVRDLWSSFR 250
ILTKEHFYHV GASEKNRSPM VEALITNIAK DIIDHIEKSS GDILVIEE 298
Length:298
Mass (Da):34,639
Last modified:March 20, 1987 - v1
Checksum:i5FF2263E733574D5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X00530 Genomic DNA. Translation: CAA25209.1.
M19941 Genomic DNA. Translation: AAA24614.1.
PIRiA00557. XYECR5.
RefSeqiNP_863581.1. NC_005019.1.
WP_011117660.1. NC_005019.1.

Genome annotation databases

GeneIDi1446622.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X00530 Genomic DNA. Translation: CAA25209.1 .
M19941 Genomic DNA. Translation: AAA24614.1 .
PIRi A00557. XYECR5.
RefSeqi NP_863581.1. NC_005019.1.
WP_011117660.1. NC_005019.1.

3D structure databases

ProteinModelPortali P04393.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3397. M.EcoRV.

Proteomic databases

PRIDEi P04393.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1446622.

Family and domain databases

Gene3Di 1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProi IPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF02086. MethyltransfD12. 1 hit.
[Graphical view ]
PIRSFi PIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSi PR00505. D12N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00571. dam. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization of the genes coding for the Eco RV restriction and modification system of Escherichia coli."
    Bougueleret L., Schwarzstein M., Tsugita A., Zabeau M.
    Nucleic Acids Res. 12:3659-3676(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The EcoRV restriction-modification system: genes, enzymes, synthetic substrates."
    Kraev A.S., Kravets A.N., Chernov B.K., Skryabin K.G., Baev A.A.
    Mol. Biol. (Mosk.) 19:236-242(1985)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-45.

Entry informationi

Entry nameiMTE5_ECOLX
AccessioniPrimary (citable) accession number: P04393
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: March 20, 1987
Last modified: September 3, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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