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P04392

- DMA_BPT4

UniProt

P04392 - DMA_BPT4

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Protein

DNA adenine methylase

Gene

DAM

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei7 – 71S-adenosyl-L-methionine
Binding sitei11 – 111S-adenosyl-L-methionine; via amide nitrogen
Binding sitei50 – 501S-adenosyl-L-methionine
Binding sitei171 – 1711S-adenosyl-L-methionine

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.72. 732.

Protein family/group databases

REBASEi2837. M.EcoT4Dam.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA adenine methylase (EC:2.1.1.72)
Alternative name(s):
Deoxyadenosyl-methyltransferase
M.EcoT4Dam
Gene namesi
Name:DAM
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostiEscherichia coli [TaxID: 562]
ProteomesiUP000009087: Genome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi126 – 1261P → S in damh; hypermethylating mutant.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 259259DNA adenine methylasePRO_0000087990Add
BLAST

Structurei

Secondary structure

1
259
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni12 – 143
Helixi15 – 206
Beta strandi26 – 305
Helixi39 – 413
Beta strandi44 – 496
Helixi53 – 6210
Helixi67 – 7610
Helixi84 – 9714
Helixi100 – 1078
Helixi111 – 1133
Helixi136 – 14611
Helixi147 – 1493
Beta strandi150 – 1534
Helixi157 – 1593
Beta strandi166 – 1705
Beta strandi175 – 1773
Helixi180 – 1845
Helixi187 – 20115
Turni202 – 2043
Beta strandi206 – 21510
Helixi221 – 2277
Beta strandi230 – 2345
Helixi237 – 2426
Beta strandi254 – 2585

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Q0SX-ray2.30A1-259[»]
1Q0TX-ray3.10A/B1-259[»]
1YF3X-ray2.29A/B1-259[»]
1YFJX-ray2.69A/B/C/D/E/F1-259[»]
1YFLX-ray3.09A/B/D/E1-259[»]
ProteinModelPortaliP04392.
SMRiP04392. Positions 1-259.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04392.

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProiIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04392-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLGAIAYTGN KQSLLPELKS HFPKYNRFVD LFCGGLSVSL NVNGPVLAND
60 70 80 90 100
IQEPIIEMYK RLINVSWDDV LKVIKQYKLS KTSKEEFLKL REDYNKTRDP
110 120 130 140 150
LLLYVLHFHG FSNMIRINDK GNFTTPFGKR TINKNSEKQY NHFKQNCDKI
160 170 180 190 200
IFSSLHFKDV KILDGDFVYV DPPYLITVAD YNKFWSEDEE KDLLNLLDSL
210 220 230 240 250
NDRGIKFGQS NVLEHHGKEN TLLKEWSKKY NVKHLNKKYV FNIYHSKEKN

GTDEVYIFN
Length:259
Mass (Da):30,417
Last modified:March 20, 1987 - v1
Checksum:iA49401D059A4388D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti139 – 1402QY → RF(PubMed:2510127)Curated
Sequence conflicti209 – 2091Q → L(PubMed:2510127)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X01416 Genomic DNA. Translation: CAA25660.1.
K03113 Genomic DNA. Translation: AAA32555.1.
X17641 Genomic DNA. No translation available.
AF158101 Genomic DNA. Translation: AAD42553.1.
PIRiA00554. XYBPT4.
RefSeqiNP_049647.1. NC_000866.4.

Genome annotation databases

GeneIDi1258548.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X01416 Genomic DNA. Translation: CAA25660.1 .
K03113 Genomic DNA. Translation: AAA32555.1 .
X17641 Genomic DNA. No translation available.
AF158101 Genomic DNA. Translation: AAD42553.1 .
PIRi A00554. XYBPT4.
RefSeqi NP_049647.1. NC_000866.4.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1Q0S X-ray 2.30 A 1-259 [» ]
1Q0T X-ray 3.10 A/B 1-259 [» ]
1YF3 X-ray 2.29 A/B 1-259 [» ]
1YFJ X-ray 2.69 A/B/C/D/E/F 1-259 [» ]
1YFL X-ray 3.09 A/B/D/E 1-259 [» ]
ProteinModelPortali P04392.
SMRi P04392. Positions 1-259.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 2837. M.EcoT4Dam.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1258548.

Enzyme and pathway databases

BRENDAi 2.1.1.72. 732.

Miscellaneous databases

EvolutionaryTracei P04392.

Family and domain databases

Gene3Di 1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProi IPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF02086. MethyltransfD12. 1 hit.
[Graphical view ]
PIRSFi PIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSi PR00505. D12N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00571. dam. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Regulation of a new bacteriophage T4 gene, 69, that spans an origin of DNA replication."
    McDonald P.M., Mosig G.
    EMBO J. 3:2863-2871(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Single amino acid changes that alter the DNA sequence specificity of the DNA-[N6-adenine] methyltransferase (Dam) of bacteriophage T4."
    Miner Z., Schlagman S.L., Hattman S.
    Nucleic Acids Res. 17:8149-8157(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTANT DAMH.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).

Entry informationi

Entry nameiDMA_BPT4
AccessioniPrimary (citable) accession number: P04392
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: March 20, 1987
Last modified: October 1, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3