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P04392

- DMA_BPT4

UniProt

P04392 - DMA_BPT4

Protein

DNA adenine methylase

Gene

DAM

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (20 Mar 1987)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei7 – 71S-adenosyl-L-methionine
    Binding sitei11 – 111S-adenosyl-L-methionine; via amide nitrogen
    Binding sitei50 – 501S-adenosyl-L-methionine
    Binding sitei171 – 1711S-adenosyl-L-methionine

    GO - Molecular functioni

    1. nucleic acid binding Source: InterPro
    2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BRENDAi2.1.1.72. 732.

    Protein family/group databases

    REBASEi2837. M.EcoT4Dam.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA adenine methylase (EC:2.1.1.72)
    Alternative name(s):
    Deoxyadenosyl-methyltransferase
    M.EcoT4Dam
    Gene namesi
    Name:DAM
    OrganismiEnterobacteria phage T4 (Bacteriophage T4)
    Taxonomic identifieri10665 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
    Virus hostiEscherichia coli [TaxID: 562]
    ProteomesiUP000009087: Genome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi126 – 1261P → S in damh; hypermethylating mutant.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 259259DNA adenine methylasePRO_0000087990Add
    BLAST

    Structurei

    Secondary structure

    1
    259
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni12 – 143
    Helixi15 – 206
    Beta strandi26 – 305
    Helixi39 – 413
    Beta strandi44 – 496
    Helixi53 – 6210
    Helixi67 – 7610
    Helixi84 – 9714
    Helixi100 – 1078
    Helixi111 – 1133
    Helixi136 – 14611
    Helixi147 – 1493
    Beta strandi150 – 1534
    Helixi157 – 1593
    Beta strandi166 – 1705
    Beta strandi175 – 1773
    Helixi180 – 1845
    Helixi187 – 20115
    Turni202 – 2043
    Beta strandi206 – 21510
    Helixi221 – 2277
    Beta strandi230 – 2345
    Helixi237 – 2426
    Beta strandi254 – 2585

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1Q0SX-ray2.30A1-259[»]
    1Q0TX-ray3.10A/B1-259[»]
    1YF3X-ray2.29A/B1-259[»]
    1YFJX-ray2.69A/B/C/D/E/F1-259[»]
    1YFLX-ray3.09A/B/D/E1-259[»]
    ProteinModelPortaliP04392.
    SMRiP04392. Positions 1-259.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP04392.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Family and domain databases

    Gene3Di1.10.1020.10. 1 hit.
    3.40.50.150. 2 hits.
    InterProiIPR023095. Ade_MeTrfase_dom_2.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR012263. M_m6A_EcoRV.
    IPR012327. MeTrfase_D12.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF02086. MethyltransfD12. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
    PRINTSiPR00505. D12N6MTFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00571. dam. 1 hit.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P04392-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLGAIAYTGN KQSLLPELKS HFPKYNRFVD LFCGGLSVSL NVNGPVLAND    50
    IQEPIIEMYK RLINVSWDDV LKVIKQYKLS KTSKEEFLKL REDYNKTRDP 100
    LLLYVLHFHG FSNMIRINDK GNFTTPFGKR TINKNSEKQY NHFKQNCDKI 150
    IFSSLHFKDV KILDGDFVYV DPPYLITVAD YNKFWSEDEE KDLLNLLDSL 200
    NDRGIKFGQS NVLEHHGKEN TLLKEWSKKY NVKHLNKKYV FNIYHSKEKN 250
    GTDEVYIFN 259
    Length:259
    Mass (Da):30,417
    Last modified:March 20, 1987 - v1
    Checksum:iA49401D059A4388D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti139 – 1402QY → RF(PubMed:2510127)Curated
    Sequence conflicti209 – 2091Q → L(PubMed:2510127)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X01416 Genomic DNA. Translation: CAA25660.1.
    K03113 Genomic DNA. Translation: AAA32555.1.
    X17641 Genomic DNA. No translation available.
    AF158101 Genomic DNA. Translation: AAD42553.1.
    PIRiA00554. XYBPT4.
    RefSeqiNP_049647.1. NC_000866.4.

    Genome annotation databases

    GeneIDi1258548.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X01416 Genomic DNA. Translation: CAA25660.1 .
    K03113 Genomic DNA. Translation: AAA32555.1 .
    X17641 Genomic DNA. No translation available.
    AF158101 Genomic DNA. Translation: AAD42553.1 .
    PIRi A00554. XYBPT4.
    RefSeqi NP_049647.1. NC_000866.4.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1Q0S X-ray 2.30 A 1-259 [» ]
    1Q0T X-ray 3.10 A/B 1-259 [» ]
    1YF3 X-ray 2.29 A/B 1-259 [» ]
    1YFJ X-ray 2.69 A/B/C/D/E/F 1-259 [» ]
    1YFL X-ray 3.09 A/B/D/E 1-259 [» ]
    ProteinModelPortali P04392.
    SMRi P04392. Positions 1-259.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 2837. M.EcoT4Dam.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1258548.

    Enzyme and pathway databases

    BRENDAi 2.1.1.72. 732.

    Miscellaneous databases

    EvolutionaryTracei P04392.

    Family and domain databases

    Gene3Di 1.10.1020.10. 1 hit.
    3.40.50.150. 2 hits.
    InterProi IPR023095. Ade_MeTrfase_dom_2.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR012263. M_m6A_EcoRV.
    IPR012327. MeTrfase_D12.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF02086. MethyltransfD12. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000398. M_m6A_EcoRV. 1 hit.
    PRINTSi PR00505. D12N6MTFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00571. dam. 1 hit.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Regulation of a new bacteriophage T4 gene, 69, that spans an origin of DNA replication."
      McDonald P.M., Mosig G.
      EMBO J. 3:2863-2871(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Single amino acid changes that alter the DNA sequence specificity of the DNA-[N6-adenine] methyltransferase (Dam) of bacteriophage T4."
      Miner Z., Schlagman S.L., Hattman S.
      Nucleic Acids Res. 17:8149-8157(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTANT DAMH.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).

    Entry informationi

    Entry nameiDMA_BPT4
    AccessioniPrimary (citable) accession number: P04392
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 20, 1987
    Last sequence update: March 20, 1987
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3