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Protein

Galactose/lactose metabolism regulatory protein GAL80

Gene

GAL80

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This protein is a negative regulator for the gene expression of the lactose/galactose metabolic genes. It binds to GAL4 and so blocks transcriptional activation by it, in the absence of an inducing sugar.1 Publication

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • kinase inhibitor activity Source: SGD
  • oxidoreductase activity Source: GO_Central
  • RNA polymerase II activating transcription factor binding Source: SGD

GO - Biological processi

  • galactose metabolic process Source: SGD
  • negative regulation of kinase activity Source: SGD
  • negative regulation of sequence-specific DNA binding transcription factor activity Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter Source: SGD
  • oxidation-reduction process Source: GO_Central
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Carbohydrate metabolism, Galactose metabolism, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32648-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Galactose/lactose metabolism regulatory protein GAL80
Gene namesi
Name:GAL80
Ordered Locus Names:YML051W
ORF Names:YM9827.01, YM9958.12
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML051W.
SGDiS000004515. GAL80.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 435435Galactose/lactose metabolism regulatory protein GAL80PRO_0000087427Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP04387.

PTM databases

iPTMnetiP04387.

Interactioni

Subunit structurei

Monomer.1 Publication

GO - Molecular functioni

  • RNA polymerase II activating transcription factor binding Source: SGD

Protein-protein interaction databases

BioGridi35117. 117 interactions.
DIPiDIP-594N.
IntActiP04387. 30 interactions.
MINTiMINT-2786797.

Structurei

Secondary structure

1
435
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi18 – 247Combined sources
Beta strandi29 – 313Combined sources
Turni32 – 354Combined sources
Helixi36 – 427Combined sources
Turni43 – 464Combined sources
Beta strandi47 – 537Combined sources
Helixi57 – 6610Combined sources
Beta strandi73 – 775Combined sources
Helixi78 – 836Combined sources
Beta strandi88 – 925Combined sources
Helixi96 – 983Combined sources
Helixi99 – 10911Combined sources
Helixi110 – 1123Combined sources
Beta strandi118 – 1247Combined sources
Helixi129 – 14012Combined sources
Beta strandi145 – 1495Combined sources
Helixi151 – 1544Combined sources
Helixi156 – 16611Combined sources
Turni167 – 1704Combined sources
Beta strandi172 – 18110Combined sources
Beta strandi183 – 1908Combined sources
Helixi195 – 1984Combined sources
Turni206 – 2094Combined sources
Helixi210 – 22213Combined sources
Beta strandi226 – 2349Combined sources
Beta strandi238 – 2436Combined sources
Beta strandi245 – 2473Combined sources
Beta strandi249 – 2557Combined sources
Beta strandi260 – 2689Combined sources
Turni269 – 2724Combined sources
Beta strandi273 – 28210Combined sources
Beta strandi287 – 2893Combined sources
Beta strandi291 – 30010Combined sources
Beta strandi302 – 3065Combined sources
Helixi312 – 3143Combined sources
Beta strandi316 – 3227Combined sources
Beta strandi348 – 3536Combined sources
Helixi360 – 37617Combined sources
Turni377 – 3804Combined sources
Beta strandi396 – 3983Combined sources
Helixi402 – 42120Combined sources
Turni428 – 4325Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3BTSX-ray2.70A/B1-435[»]
3BTUX-ray2.85A/B/C/D/E/F1-435[»]
3BTVX-ray2.10A/B1-435[»]
3V2UX-ray2.10A/B1-435[»]
ProteinModelPortaliP04387.
SMRiP04387. Positions 15-435.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04387.

Family & Domainsi

Sequence similaritiesi

To K.lactis GAL80.Curated

Phylogenomic databases

HOGENOMiHOG000000744.
InParanoidiP04387.
OMAiGRKSPYI.
OrthoDBiEOG092C37EF.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000683. Oxidoreductase_N.
[Graphical view]
PfamiPF01408. GFO_IDH_MocA. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 2 hits.

Sequencei

Sequence statusi: Complete.

P04387-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYNKRSSVS TVPNAAPIRV GFVGLNAAKG WAIKTHYPAI LQLSSQFQIT
60 70 80 90 100
ALYSPKIETS IATIQRLKLS NATAFPTLES FASSSTIDMI VIAIQVASHY
110 120 130 140 150
EVVMPLLEFS KNNPNLKYLF VEWALACSLD QAESIYKAAA ERGVQTIISL
160 170 180 190 200
QGRKSPYILR AKELISQGYI GDINSIEIAG NGGWYGYERP VKSPKYIYEI
210 220 230 240 250
GNGVDLVTTT FGHTIDILQY MTSSYFSRIN AMVFNNIPEQ ELIDERGNRL
260 270 280 290 300
GQRVPKTVPD HLLFQGTLLN GNVPVSCSFK GGKPTKKFTK NLVIDIHGTK
310 320 330 340 350
GDLKLEGDAG FAEISNLVLY YSGTRANDFP LANGQQAPLD PGYDAGKEIM
360 370 380 390 400
EVYHLRNYNA IVGNIHRLYQ SISDFHFNTK KIPELPSQFV MQGFDFEGFP
410 420 430
TLMDALILHR LIESVYKSNM MGSTLNVSNI SHYSL
Length:435
Mass (Da):48,323
Last modified:October 1, 1996 - v2
Checksum:i1492473684F3894C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti101 – 1011E → D in CAA25827 (PubMed:6393054).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01667 Genomic DNA. Translation: CAA25827.1.
Z46729 Genomic DNA. Translation: CAA86725.1.
Z47816 Genomic DNA. Translation: CAA87823.1.
BK006946 Genomic DNA. Translation: DAA09848.1.
PIRiA03606. RGBYG8.
RefSeqiNP_013661.1. NM_001182409.1.

Genome annotation databases

EnsemblFungiiYML051W; YML051W; YML051W.
GeneIDi854954.
KEGGisce:YML051W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01667 Genomic DNA. Translation: CAA25827.1.
Z46729 Genomic DNA. Translation: CAA86725.1.
Z47816 Genomic DNA. Translation: CAA87823.1.
BK006946 Genomic DNA. Translation: DAA09848.1.
PIRiA03606. RGBYG8.
RefSeqiNP_013661.1. NM_001182409.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3BTSX-ray2.70A/B1-435[»]
3BTUX-ray2.85A/B/C/D/E/F1-435[»]
3BTVX-ray2.10A/B1-435[»]
3V2UX-ray2.10A/B1-435[»]
ProteinModelPortaliP04387.
SMRiP04387. Positions 15-435.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35117. 117 interactions.
DIPiDIP-594N.
IntActiP04387. 30 interactions.
MINTiMINT-2786797.

PTM databases

iPTMnetiP04387.

Proteomic databases

MaxQBiP04387.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML051W; YML051W; YML051W.
GeneIDi854954.
KEGGisce:YML051W.

Organism-specific databases

EuPathDBiFungiDB:YML051W.
SGDiS000004515. GAL80.

Phylogenomic databases

HOGENOMiHOG000000744.
InParanoidiP04387.
OMAiGRKSPYI.
OrthoDBiEOG092C37EF.

Enzyme and pathway databases

BioCyciYEAST:G3O-32648-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP04387.
PROiP04387.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000683. Oxidoreductase_N.
[Graphical view]
PfamiPF01408. GFO_IDH_MocA. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiGAL80_YEAST
AccessioniPrimary (citable) accession number: P04387
Secondary accession number(s): D6VZC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 784 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.