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P04386

- GAL4_YEAST

UniProt

P04386 - GAL4_YEAST

Protein

Regulatory protein GAL4

Gene

GAL4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 154 (01 Oct 2014)
      Sequence version 2 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which code for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi11 – 111Zinc 1
    Metal bindingi11 – 111Zinc 2
    Metal bindingi14 – 141Zinc 1
    Metal bindingi21 – 211Zinc 1
    Metal bindingi28 – 281Zinc 1
    Metal bindingi28 – 281Zinc 2
    Metal bindingi31 – 311Zinc 2
    Metal bindingi38 – 381Zinc 2

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi11 – 3828Zn(2)-C6 fungal-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: SGD
    2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: SGD
    3. RNA polymerase II transcription factor binding Source: SGD
    4. sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity Source: SGD
    5. zinc ion binding Source: InterPro

    GO - Biological processi

    1. galactose metabolic process Source: SGD
    2. positive regulation of transcription from RNA polymerase II promoter by galactose Source: SGD

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Carbohydrate metabolism, Galactose metabolism, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34134-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Regulatory protein GAL4
    Gene namesi
    Name:GAL4
    Ordered Locus Names:YPL248C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPL248c.
    SGDiS000006169. GAL4.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi26 – 261P → L: Loss of DNA-binding. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 881881Regulatory protein GAL4PRO_0000114951Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei694 – 6941Phosphotyrosine1 Publication
    Modified residuei696 – 6961Phosphoserine1 Publication
    Modified residuei699 – 6991Phosphoserine1 Publication
    Modified residuei703 – 7031Phosphoserine1 Publication
    Modified residuei712 – 7121Phosphoserine1 Publication

    Post-translational modificationi

    Association between GAL11 and GAL4 may serve to expedite phosphorylation of GAL4.2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP04386.
    PeptideAtlasiP04386.

    Expressioni

    Gene expression databases

    GenevestigatoriP04386.

    Interactioni

    Subunit structurei

    Binds DNA as a homodimer. Interacts directly with the mediator subunits GAL11/MED15 and SRB4/MED17.2 Publications

    Protein-protein interaction databases

    BioGridi35915. 87 interactions.
    DIPiDIP-593N.
    IntActiP04386. 5 interactions.
    MINTiMINT-1510289.
    STRINGi4932.YPL248C.

    Structurei

    Secondary structure

    1
    881
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi12 – 176
    Beta strandi25 – 273
    Helixi29 – 335
    Helixi51 – 7121
    Beta strandi73 – 753
    Helixi77 – 826
    Helixi86 – 938

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1AW6NMR-A1-43[»]
    1D66X-ray2.70A/B1-65[»]
    1HBWNMR-A/B50-106[»]
    3BTSX-ray2.70E/F854-874[»]
    3COQX-ray2.40A/B8-96[»]
    ProteinModelPortaliP04386.
    SMRiP04386. Positions 8-96.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP04386.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi862 – 87099aaTAD

    Domaini

    the 9aaTAD motif (residues 862 to 870) is a transactivation domain present in a large number of yeast and animal transcription factors.1 Publication

    Sequence similaritiesi

    Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG85363.
    HOGENOMiHOG000112689.
    KOiK09241.
    OMAiIYESELT.
    OrthoDBiEOG7J44JV.

    Family and domain databases

    Gene3Di4.10.240.10. 1 hit.
    InterProiIPR005600. Gal4_dimer_dom.
    IPR007219. Transcription_factor_dom_fun.
    IPR001138. Zn2-C6_fun-type_DNA-bd.
    [Graphical view]
    PfamiPF04082. Fungal_trans. 1 hit.
    PF03902. Gal4_dimer. 1 hit.
    PF00172. Zn_clus. 1 hit.
    [Graphical view]
    SMARTiSM00906. Fungal_trans. 1 hit.
    SM00066. GAL4. 1 hit.
    [Graphical view]
    SUPFAMiSSF57701. SSF57701. 1 hit.
    PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
    PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P04386-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLLSSIEQA CDICRLKKLK CSKEKPKCAK CLKNNWECRY SPKTKRSPLT    50
    RAHLTEVESR LERLEQLFLL IFPREDLDMI LKMDSLQDIK ALLTGLFVQD 100
    NVNKDAVTDR LASVETDMPL TLRQHRISAT SSSEESSNKG QRQLTVSIDS 150
    AAHHDNSTIP LDFMPRDALH GFDWSEEDDM SDGLPFLKTD PNNNGFFGDG 200
    SLLCILRSIG FKPENYTNSN VNRLPTMITD RYTLASRSTT SRLLQSYLNN 250
    FHPYCPIVHS PTLMMLYNNQ IEIASKDQWQ ILFNCILAIG AWCIEGESTD 300
    IDVFYYQNAK SHLTSKVFES GSIILVTALH LLSRYTQWRQ KTNTSYNFHS 350
    FSIRMAISLG LNRDLPSSFS DSSILEQRRR IWWSVYSWEI QLSLLYGRSI 400
    QLSQNTISFP SSVDDVQRTT TGPTIYHGII ETARLLQVFT KIYELDKTVT 450
    AEKSPICAKK CLMICNEIEE VSRQAPKFLQ MDISTTALTN LLKEHPWLSF 500
    TRFELKWKQL SLIIYVLRDF FTNFTQKKSQ LEQDQNDHQS YEVKRCSIML 550
    SDAAQRTVMS VSSYMDNHNV TPYFAWNCSY YLFNAVLVPI KTLLSNSKSN 600
    AENNETAQLL QQINTVLMLL KKLATFKIQT CEKYIQVLEE VCAPFLLSQC 650
    AIPLPHISYN NSNGSAIKNI VGSATIAQYP TLPEENVNNI SVKYVSPGSV 700
    GPSPVPLKSG ASFSDLVKLL SNRPPSRNSP VTIPRSTPSH RSVTPFLGQQ 750
    QQLQSLVPLT PSALFGGANF NQSGNIADSS LSFTFTNSSN GPNLITTQTN 800
    SQALSQPIAS SNVHDNFMNN EITASKIDDG NNSKPLSPGW TDQTAYNAFG 850
    ITTGMFNTTT MDDVYNYLFD DEDTPPNPKK E 881
    Length:881
    Mass (Da):99,403
    Last modified:November 1, 1995 - v2
    Checksum:i29D7FF68B0B05880
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K01486 Genomic DNA. Translation: AAA34626.1.
    Z67751 Genomic DNA. Translation: CAA91596.1.
    Z73604 Genomic DNA. Translation: CAA97969.1.
    BK006949 Genomic DNA. Translation: DAA11189.1.
    PIRiA05022. RGBYG4.
    RefSeqiNP_015076.1. NM_001184062.1.

    Genome annotation databases

    EnsemblFungiiYPL248C; YPL248C; YPL248C.
    GeneIDi855828.
    KEGGisce:YPL248C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K01486 Genomic DNA. Translation: AAA34626.1 .
    Z67751 Genomic DNA. Translation: CAA91596.1 .
    Z73604 Genomic DNA. Translation: CAA97969.1 .
    BK006949 Genomic DNA. Translation: DAA11189.1 .
    PIRi A05022. RGBYG4.
    RefSeqi NP_015076.1. NM_001184062.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1AW6 NMR - A 1-43 [» ]
    1D66 X-ray 2.70 A/B 1-65 [» ]
    1HBW NMR - A/B 50-106 [» ]
    3BTS X-ray 2.70 E/F 854-874 [» ]
    3COQ X-ray 2.40 A/B 8-96 [» ]
    ProteinModelPortali P04386.
    SMRi P04386. Positions 8-96.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35915. 87 interactions.
    DIPi DIP-593N.
    IntActi P04386. 5 interactions.
    MINTi MINT-1510289.
    STRINGi 4932.YPL248C.

    Proteomic databases

    PaxDbi P04386.
    PeptideAtlasi P04386.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPL248C ; YPL248C ; YPL248C .
    GeneIDi 855828.
    KEGGi sce:YPL248C.

    Organism-specific databases

    CYGDi YPL248c.
    SGDi S000006169. GAL4.

    Phylogenomic databases

    eggNOGi NOG85363.
    HOGENOMi HOG000112689.
    KOi K09241.
    OMAi IYESELT.
    OrthoDBi EOG7J44JV.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34134-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P04386.
    NextBioi 980383.

    Gene expression databases

    Genevestigatori P04386.

    Family and domain databases

    Gene3Di 4.10.240.10. 1 hit.
    InterProi IPR005600. Gal4_dimer_dom.
    IPR007219. Transcription_factor_dom_fun.
    IPR001138. Zn2-C6_fun-type_DNA-bd.
    [Graphical view ]
    Pfami PF04082. Fungal_trans. 1 hit.
    PF03902. Gal4_dimer. 1 hit.
    PF00172. Zn_clus. 1 hit.
    [Graphical view ]
    SMARTi SM00906. Fungal_trans. 1 hit.
    SM00066. GAL4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57701. SSF57701. 1 hit.
    PROSITEi PS00463. ZN2_CY6_FUNGAL_1. 1 hit.
    PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of the Saccharomyces cerevisiae GAL4 gene."
      Laughon A., Gesteland R.F.
      Mol. Cell. Biol. 4:260-267(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Genetic evidence that zinc is an essential co-factor in the DNA binding domain of GAL4 protein."
      Johnston M.
      Nature 328:353-355(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS, ZINC REQUIREMENT.
    5. "An activator target in the RNA polymerase II holoenzyme."
      Koh S.S., Ansari A.Z., Ptashne M., Young R.A.
      Mol. Cell 1:895-904(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SRB4.
    6. "Evidence that Gal11 protein is a target of the Gal4 activation domain in the mediator."
      Jeong C.-J., Yang S.-H., Xie Y., Zhang L., Johnston S.A., Kodadek T.
      Biochemistry 40:9421-9427(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GAL11.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Nine-amino-acid transactivation domain: establishment and prediction utilities."
      Piskacek S., Gregor M., Nemethova M., Grabner M., Kovarik P., Piskacek M.
      Genomics 89:756-768(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: DOMAIN.
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-712, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-694; SER-696; SER-699 AND SER-703, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "DNA recognition by GAL4: structure of a protein-DNA complex."
      Marmorstein R., Carey M., Ptashne M., Harrison S.C.
      Nature 356:408-414(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 1-65.
    12. "GAL4 transcription factor is not a 'zinc finger' but forms a Zn(II)2Cys6 binuclear cluster."
      Pan T., Coleman J.E.
      Proc. Natl. Acad. Sci. U.S.A. 87:2077-2081(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF ZINC-BINDING REGION.
    13. "Sequential assignments of the 1H NMR resonances of Zn(II)2 and 113Cd(II)2 derivatives of the DNA-binding domain of the GAL4 transcription factor reveal a novel structural motif for specific DNA recognition."
      Pan T., Coleman J.E.
      Biochemistry 30:4212-4222(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF ZINC-BINDING REGION.
    14. "Complete assignment of the 1H NMR spectrum and secondary structure of the DNA binding domain of GAL4."
      Gadhavi P.L., Raine A.R.C., Alefounder P.R., Laue E.D.
      FEBS Lett. 276:49-53(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF ZINC-BINDING REGION.
    15. "Structure of the DNA-binding domain of zinc GAL4."
      Kraulis P.J., Raine A.R.C., Gadhavi P.L., Laue E.D.
      Nature 356:448-450(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF ZINC-BINDING REGION.
    16. "Solution structure of the DNA-binding domain of Cd2-GAL4 from S. cerevisiae."
      Baleja J.D., Marmorstein R., Harrison S.C., Wagner G.
      Nature 356:450-453(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF ZINC-BINDING REGION.

    Entry informationi

    Entry nameiGAL4_YEAST
    AccessioniPrimary (citable) accession number: P04386
    Secondary accession number(s): D6W3C3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 20, 1987
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 154 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 166 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3