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Protein

Regulatory protein GAL4

Gene

GAL4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which code for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes.

Miscellaneous

Present with 166 molecules/cell in log phase SD medium.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi11Zinc 11
Metal bindingi11Zinc 21
Metal bindingi14Zinc 11
Metal bindingi21Zinc 11
Metal bindingi28Zinc 11
Metal bindingi28Zinc 21
Metal bindingi31Zinc 21
Metal bindingi38Zinc 21

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi11 – 38Zn(2)-C6 fungal-typePROSITE-ProRule annotationAdd BLAST28

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: SGD
  • RNA polymerase II transcription factor activity, sequence-specific transcription regulatory region DNA binding Source: SGD
  • RNA polymerase II transcription factor binding Source: SGD
  • transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • galactose metabolic process Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter by galactose Source: SGD

Keywordsi

Molecular functionActivator, DNA-binding
Biological processCarbohydrate metabolism, Galactose metabolism, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-34134-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Regulatory protein GAL4
Gene namesi
Name:GAL4
Ordered Locus Names:YPL248C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL248C
SGDiS000006169 GAL4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi26P → L: Loss of DNA-binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001149511 – 881Regulatory protein GAL4Add BLAST881

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei694PhosphotyrosineCombined sources1
Modified residuei696PhosphoserineCombined sources1
Modified residuei699PhosphoserineCombined sources1
Modified residuei703PhosphoserineCombined sources1
Modified residuei712PhosphoserineCombined sources1

Post-translational modificationi

Association between GAL11 and GAL4 may serve to expedite phosphorylation of GAL4.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP04386

PTM databases

iPTMnetiP04386

Interactioni

Subunit structurei

Binds DNA as a homodimer. Interacts directly with the mediator subunits GAL11/MED15 and SRB4/MED17.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • RNA polymerase II transcription factor binding Source: SGD

Protein-protein interaction databases

BioGridi35915, 153 interactors
DIPiDIP-593N
IntActiP04386, 6 interactors
MINTiP04386
STRINGi4932.YPL248C

Structurei

Secondary structure

1881
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 17Combined sources6
Beta strandi25 – 27Combined sources3
Helixi29 – 33Combined sources5
Helixi51 – 71Combined sources21
Beta strandi73 – 75Combined sources3
Helixi77 – 82Combined sources6
Helixi86 – 93Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AW6NMR-A1-43[»]
1D66X-ray2.70A/B1-65[»]
1HBWNMR-A/B50-106[»]
3BTSX-ray2.70E/F854-874[»]
3COQX-ray2.40A/B8-96[»]
ProteinModelPortaliP04386
SMRiP04386
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04386

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi862 – 8709aaTAD9

Domaini

the 9aaTAD motif (residues 862 to 870) is a transactivation domain present in a large number of yeast and animal transcription factors.1 Publication

Phylogenomic databases

HOGENOMiHOG000112689
InParanoidiP04386
KOiK09241
OMAiETTVYTH
OrthoDBiEOG092C4I3T

Family and domain databases

CDDicd00067 GAL4, 1 hit
cd14654 ZIP_Gal4, 1 hit
Gene3Di4.10.240.10, 1 hit
InterProiView protein in InterPro
IPR005600 Gal4_dimer_dom
IPR007219 Transcription_factor_dom_fun
IPR001138 Zn2-C6_fun-type_DNA-bd
IPR036864 Zn2-C6_fun-type_DNA-bd_sf
PfamiView protein in Pfam
PF04082 Fungal_trans, 1 hit
PF03902 Gal4_dimer, 1 hit
PF00172 Zn_clus, 1 hit
SMARTiView protein in SMART
SM00906 Fungal_trans, 1 hit
SM00066 GAL4, 1 hit
SUPFAMiSSF57701 SSF57701, 1 hit
PROSITEiView protein in PROSITE
PS00463 ZN2_CY6_FUNGAL_1, 1 hit
PS50048 ZN2_CY6_FUNGAL_2, 1 hit

Sequencei

Sequence statusi: Complete.

P04386-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLSSIEQA CDICRLKKLK CSKEKPKCAK CLKNNWECRY SPKTKRSPLT
60 70 80 90 100
RAHLTEVESR LERLEQLFLL IFPREDLDMI LKMDSLQDIK ALLTGLFVQD
110 120 130 140 150
NVNKDAVTDR LASVETDMPL TLRQHRISAT SSSEESSNKG QRQLTVSIDS
160 170 180 190 200
AAHHDNSTIP LDFMPRDALH GFDWSEEDDM SDGLPFLKTD PNNNGFFGDG
210 220 230 240 250
SLLCILRSIG FKPENYTNSN VNRLPTMITD RYTLASRSTT SRLLQSYLNN
260 270 280 290 300
FHPYCPIVHS PTLMMLYNNQ IEIASKDQWQ ILFNCILAIG AWCIEGESTD
310 320 330 340 350
IDVFYYQNAK SHLTSKVFES GSIILVTALH LLSRYTQWRQ KTNTSYNFHS
360 370 380 390 400
FSIRMAISLG LNRDLPSSFS DSSILEQRRR IWWSVYSWEI QLSLLYGRSI
410 420 430 440 450
QLSQNTISFP SSVDDVQRTT TGPTIYHGII ETARLLQVFT KIYELDKTVT
460 470 480 490 500
AEKSPICAKK CLMICNEIEE VSRQAPKFLQ MDISTTALTN LLKEHPWLSF
510 520 530 540 550
TRFELKWKQL SLIIYVLRDF FTNFTQKKSQ LEQDQNDHQS YEVKRCSIML
560 570 580 590 600
SDAAQRTVMS VSSYMDNHNV TPYFAWNCSY YLFNAVLVPI KTLLSNSKSN
610 620 630 640 650
AENNETAQLL QQINTVLMLL KKLATFKIQT CEKYIQVLEE VCAPFLLSQC
660 670 680 690 700
AIPLPHISYN NSNGSAIKNI VGSATIAQYP TLPEENVNNI SVKYVSPGSV
710 720 730 740 750
GPSPVPLKSG ASFSDLVKLL SNRPPSRNSP VTIPRSTPSH RSVTPFLGQQ
760 770 780 790 800
QQLQSLVPLT PSALFGGANF NQSGNIADSS LSFTFTNSSN GPNLITTQTN
810 820 830 840 850
SQALSQPIAS SNVHDNFMNN EITASKIDDG NNSKPLSPGW TDQTAYNAFG
860 870 880
ITTGMFNTTT MDDVYNYLFD DEDTPPNPKK E
Length:881
Mass (Da):99,403
Last modified:November 1, 1995 - v2
Checksum:i29D7FF68B0B05880
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01486 Genomic DNA Translation: AAA34626.1
Z67751 Genomic DNA Translation: CAA91596.1
Z73604 Genomic DNA Translation: CAA97969.1
BK006949 Genomic DNA Translation: DAA11189.1
PIRiA05022 RGBYG4
RefSeqiNP_015076.1, NM_001184062.1

Genome annotation databases

EnsemblFungiiYPL248C; YPL248C; YPL248C
GeneIDi855828
KEGGisce:YPL248C

Similar proteinsi

Entry informationi

Entry nameiGAL4_YEAST
AccessioniPrimary (citable) accession number: P04386
Secondary accession number(s): D6W3C3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 182 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

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