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P04386

- GAL4_YEAST

UniProt

P04386 - GAL4_YEAST

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Protein

Regulatory protein GAL4

Gene

GAL4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which code for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi11 – 111Zinc 1
Metal bindingi11 – 111Zinc 2
Metal bindingi14 – 141Zinc 1
Metal bindingi21 – 211Zinc 1
Metal bindingi28 – 281Zinc 1
Metal bindingi28 – 281Zinc 2
Metal bindingi31 – 311Zinc 2
Metal bindingi38 – 381Zinc 2

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi11 – 3828Zn(2)-C6 fungal-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: SGD
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: SGD
  3. RNA polymerase II transcription factor binding Source: SGD
  4. sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity Source: SGD
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. galactose metabolic process Source: SGD
  2. positive regulation of transcription from RNA polymerase II promoter by galactose Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Carbohydrate metabolism, Galactose metabolism, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-34134-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Regulatory protein GAL4
Gene namesi
Name:GAL4
Ordered Locus Names:YPL248C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPL248c.
SGDiS000006169. GAL4.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi26 – 261P → L: Loss of DNA-binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 881881Regulatory protein GAL4PRO_0000114951Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei694 – 6941Phosphotyrosine1 Publication
Modified residuei696 – 6961Phosphoserine1 Publication
Modified residuei699 – 6991Phosphoserine1 Publication
Modified residuei703 – 7031Phosphoserine1 Publication
Modified residuei712 – 7121Phosphoserine1 Publication

Post-translational modificationi

Association between GAL11 and GAL4 may serve to expedite phosphorylation of GAL4.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP04386.
PeptideAtlasiP04386.

Expressioni

Gene expression databases

GenevestigatoriP04386.

Interactioni

Subunit structurei

Binds DNA as a homodimer. Interacts directly with the mediator subunits GAL11/MED15 and SRB4/MED17.2 Publications

Protein-protein interaction databases

BioGridi35915. 88 interactions.
DIPiDIP-593N.
IntActiP04386. 5 interactions.
MINTiMINT-1510289.
STRINGi4932.YPL248C.

Structurei

Secondary structure

1
881
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi12 – 176
Beta strandi25 – 273
Helixi29 – 335
Helixi51 – 7121
Beta strandi73 – 753
Helixi77 – 826
Helixi86 – 938

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AW6NMR-A1-43[»]
1D66X-ray2.70A/B1-65[»]
1HBWNMR-A/B50-106[»]
3BTSX-ray2.70E/F854-874[»]
3COQX-ray2.40A/B8-96[»]
ProteinModelPortaliP04386.
SMRiP04386. Positions 8-96.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04386.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi862 – 87099aaTAD

Domaini

the 9aaTAD motif (residues 862 to 870) is a transactivation domain present in a large number of yeast and animal transcription factors.1 Publication

Sequence similaritiesi

Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG85363.
HOGENOMiHOG000112689.
InParanoidiP04386.
KOiK09241.
OMAiIYESELT.
OrthoDBiEOG7J44JV.

Family and domain databases

Gene3Di4.10.240.10. 1 hit.
InterProiIPR005600. Gal4_dimer_dom.
IPR007219. Transcription_factor_dom_fun.
IPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
PfamiPF04082. Fungal_trans. 1 hit.
PF03902. Gal4_dimer. 1 hit.
PF00172. Zn_clus. 1 hit.
[Graphical view]
SMARTiSM00906. Fungal_trans. 1 hit.
SM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMiSSF57701. SSF57701. 1 hit.
PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04386-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLLSSIEQA CDICRLKKLK CSKEKPKCAK CLKNNWECRY SPKTKRSPLT
60 70 80 90 100
RAHLTEVESR LERLEQLFLL IFPREDLDMI LKMDSLQDIK ALLTGLFVQD
110 120 130 140 150
NVNKDAVTDR LASVETDMPL TLRQHRISAT SSSEESSNKG QRQLTVSIDS
160 170 180 190 200
AAHHDNSTIP LDFMPRDALH GFDWSEEDDM SDGLPFLKTD PNNNGFFGDG
210 220 230 240 250
SLLCILRSIG FKPENYTNSN VNRLPTMITD RYTLASRSTT SRLLQSYLNN
260 270 280 290 300
FHPYCPIVHS PTLMMLYNNQ IEIASKDQWQ ILFNCILAIG AWCIEGESTD
310 320 330 340 350
IDVFYYQNAK SHLTSKVFES GSIILVTALH LLSRYTQWRQ KTNTSYNFHS
360 370 380 390 400
FSIRMAISLG LNRDLPSSFS DSSILEQRRR IWWSVYSWEI QLSLLYGRSI
410 420 430 440 450
QLSQNTISFP SSVDDVQRTT TGPTIYHGII ETARLLQVFT KIYELDKTVT
460 470 480 490 500
AEKSPICAKK CLMICNEIEE VSRQAPKFLQ MDISTTALTN LLKEHPWLSF
510 520 530 540 550
TRFELKWKQL SLIIYVLRDF FTNFTQKKSQ LEQDQNDHQS YEVKRCSIML
560 570 580 590 600
SDAAQRTVMS VSSYMDNHNV TPYFAWNCSY YLFNAVLVPI KTLLSNSKSN
610 620 630 640 650
AENNETAQLL QQINTVLMLL KKLATFKIQT CEKYIQVLEE VCAPFLLSQC
660 670 680 690 700
AIPLPHISYN NSNGSAIKNI VGSATIAQYP TLPEENVNNI SVKYVSPGSV
710 720 730 740 750
GPSPVPLKSG ASFSDLVKLL SNRPPSRNSP VTIPRSTPSH RSVTPFLGQQ
760 770 780 790 800
QQLQSLVPLT PSALFGGANF NQSGNIADSS LSFTFTNSSN GPNLITTQTN
810 820 830 840 850
SQALSQPIAS SNVHDNFMNN EITASKIDDG NNSKPLSPGW TDQTAYNAFG
860 870 880
ITTGMFNTTT MDDVYNYLFD DEDTPPNPKK E
Length:881
Mass (Da):99,403
Last modified:November 1, 1995 - v2
Checksum:i29D7FF68B0B05880
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K01486 Genomic DNA. Translation: AAA34626.1.
Z67751 Genomic DNA. Translation: CAA91596.1.
Z73604 Genomic DNA. Translation: CAA97969.1.
BK006949 Genomic DNA. Translation: DAA11189.1.
PIRiA05022. RGBYG4.
RefSeqiNP_015076.1. NM_001184062.1.

Genome annotation databases

EnsemblFungiiYPL248C; YPL248C; YPL248C.
GeneIDi855828.
KEGGisce:YPL248C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K01486 Genomic DNA. Translation: AAA34626.1 .
Z67751 Genomic DNA. Translation: CAA91596.1 .
Z73604 Genomic DNA. Translation: CAA97969.1 .
BK006949 Genomic DNA. Translation: DAA11189.1 .
PIRi A05022. RGBYG4.
RefSeqi NP_015076.1. NM_001184062.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1AW6 NMR - A 1-43 [» ]
1D66 X-ray 2.70 A/B 1-65 [» ]
1HBW NMR - A/B 50-106 [» ]
3BTS X-ray 2.70 E/F 854-874 [» ]
3COQ X-ray 2.40 A/B 8-96 [» ]
ProteinModelPortali P04386.
SMRi P04386. Positions 8-96.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35915. 88 interactions.
DIPi DIP-593N.
IntActi P04386. 5 interactions.
MINTi MINT-1510289.
STRINGi 4932.YPL248C.

Proteomic databases

PaxDbi P04386.
PeptideAtlasi P04386.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPL248C ; YPL248C ; YPL248C .
GeneIDi 855828.
KEGGi sce:YPL248C.

Organism-specific databases

CYGDi YPL248c.
SGDi S000006169. GAL4.

Phylogenomic databases

eggNOGi NOG85363.
HOGENOMi HOG000112689.
InParanoidi P04386.
KOi K09241.
OMAi IYESELT.
OrthoDBi EOG7J44JV.

Enzyme and pathway databases

BioCyci YEAST:G3O-34134-MONOMER.

Miscellaneous databases

EvolutionaryTracei P04386.
NextBioi 980383.

Gene expression databases

Genevestigatori P04386.

Family and domain databases

Gene3Di 4.10.240.10. 1 hit.
InterProi IPR005600. Gal4_dimer_dom.
IPR007219. Transcription_factor_dom_fun.
IPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view ]
Pfami PF04082. Fungal_trans. 1 hit.
PF03902. Gal4_dimer. 1 hit.
PF00172. Zn_clus. 1 hit.
[Graphical view ]
SMARTi SM00906. Fungal_trans. 1 hit.
SM00066. GAL4. 1 hit.
[Graphical view ]
SUPFAMi SSF57701. SSF57701. 1 hit.
PROSITEi PS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structure of the Saccharomyces cerevisiae GAL4 gene."
    Laughon A., Gesteland R.F.
    Mol. Cell. Biol. 4:260-267(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Genetic evidence that zinc is an essential co-factor in the DNA binding domain of GAL4 protein."
    Johnston M.
    Nature 328:353-355(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS, ZINC REQUIREMENT.
  5. "An activator target in the RNA polymerase II holoenzyme."
    Koh S.S., Ansari A.Z., Ptashne M., Young R.A.
    Mol. Cell 1:895-904(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SRB4.
  6. "Evidence that Gal11 protein is a target of the Gal4 activation domain in the mediator."
    Jeong C.-J., Yang S.-H., Xie Y., Zhang L., Johnston S.A., Kodadek T.
    Biochemistry 40:9421-9427(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GAL11.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Nine-amino-acid transactivation domain: establishment and prediction utilities."
    Piskacek S., Gregor M., Nemethova M., Grabner M., Kovarik P., Piskacek M.
    Genomics 89:756-768(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-712, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-694; SER-696; SER-699 AND SER-703, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "DNA recognition by GAL4: structure of a protein-DNA complex."
    Marmorstein R., Carey M., Ptashne M., Harrison S.C.
    Nature 356:408-414(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 1-65.
  12. "GAL4 transcription factor is not a 'zinc finger' but forms a Zn(II)2Cys6 binuclear cluster."
    Pan T., Coleman J.E.
    Proc. Natl. Acad. Sci. U.S.A. 87:2077-2081(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF ZINC-BINDING REGION.
  13. "Sequential assignments of the 1H NMR resonances of Zn(II)2 and 113Cd(II)2 derivatives of the DNA-binding domain of the GAL4 transcription factor reveal a novel structural motif for specific DNA recognition."
    Pan T., Coleman J.E.
    Biochemistry 30:4212-4222(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF ZINC-BINDING REGION.
  14. "Complete assignment of the 1H NMR spectrum and secondary structure of the DNA binding domain of GAL4."
    Gadhavi P.L., Raine A.R.C., Alefounder P.R., Laue E.D.
    FEBS Lett. 276:49-53(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF ZINC-BINDING REGION.
  15. "Structure of the DNA-binding domain of zinc GAL4."
    Kraulis P.J., Raine A.R.C., Gadhavi P.L., Laue E.D.
    Nature 356:448-450(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF ZINC-BINDING REGION.
  16. "Solution structure of the DNA-binding domain of Cd2-GAL4 from S. cerevisiae."
    Baleja J.D., Marmorstein R., Harrison S.C., Wagner G.
    Nature 356:450-453(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF ZINC-BINDING REGION.

Entry informationi

Entry nameiGAL4_YEAST
AccessioniPrimary (citable) accession number: P04386
Secondary accession number(s): D6W3C3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 155 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 166 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3