Reviewed,
UniProtKB/Swiss-Prot P04386 (GAL4_YEAST)
Last modified
November 3, 2009.
Version 113.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Regulatory protein GAL4 | ||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||
| Taxonomic identifier | 4932 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 881 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which encode for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes. |
| Subunit structure | Binds DNA as a homodimer. Interacts directly with the mediator subunits GAL11/MED15 and SRB4/MED17. Ref.4 Ref.5 |
| Subcellular location | |
| Post-translational modification | Association between GAL11 and GAL4 may serve to expedite phosphorylation of GAL4. |
| Miscellaneous | Present with 166 molecules/cell in log phase SD medium. Ref.6 |
| Sequence similarities | Contains 1 Zn(2)-C6 fungal-type DNA-binding domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Carbohydrate metabolism Galactose metabolism Transcription Transcription regulation |
| Cellular component | Nucleus |
| Ligand | DNA-binding Metal-binding Zinc |
| Molecular function | Activator |
| PTM | Phosphoprotein |
| Technical term | 3D-structure Complete proteome |
| Gene Ontology (GO) | |
| Biological process | galactose metabolic process Inferred from mutant phenotype. Source: SGD positive regulation of transcription by galactoseInferred from direct assay. Source: SGD transcriptionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | nucleus Inferred from direct assay. Source: SGD |
| Molecular function | sequence-specific DNA binding Inferred from direct assay. Source: SGD transcription activator activityInferred from direct assay. Source: SGD transcription factor activityInferred from direct assay. Source: SGD zinc ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||||||||||||||
Molecule processing | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 881 | 881 | Regulatory protein GAL4 | PRO_0000114951 | |||||||||||||||||||
Regions | |||||||||||||||||||||||
| DNA binding | 11 – 38 | 28 | Zn(2)-C6 fungal-type | ||||||||||||||||||||
Sites | |||||||||||||||||||||||
| Metal binding | 11 | 1 | Zinc 1 | ||||||||||||||||||||
| Metal binding | 11 | 1 | Zinc 2 | ||||||||||||||||||||
| Metal binding | 14 | 1 | Zinc 1 | ||||||||||||||||||||
| Metal binding | 21 | 1 | Zinc 1 | ||||||||||||||||||||
| Metal binding | 28 | 1 | Zinc 1 | ||||||||||||||||||||
| Metal binding | 28 | 1 | Zinc 2 | ||||||||||||||||||||
| Metal binding | 31 | 1 | Zinc 2 | ||||||||||||||||||||
| Metal binding | 38 | 1 | Zinc 2 | ||||||||||||||||||||
Amino acid modifications | |||||||||||||||||||||||
| Modified residue | 712 | 1 | Phosphoserine Ref.7 | ||||||||||||||||||||
Experimental info | |||||||||||||||||||||||
| Mutagenesis | 26 | 1 | P → L: Loss of DNA-binding. | ||||||||||||||||||||
Secondary structure | |||||||||||||||||||||||
Helix Strand Turn | |||||||||||||||||||||||
| Helix | 12 – 17 | 6 | |||||||||||||||||||||
| Beta strand | 25 – 27 | 3 | |||||||||||||||||||||
| Helix | 29 – 33 | 5 | |||||||||||||||||||||
| Helix | 51 – 71 | 21 | |||||||||||||||||||||
| Beta strand | 73 – 75 | 3 | |||||||||||||||||||||
| Helix | 77 – 82 | 6 | |||||||||||||||||||||
| Helix | 86 – 93 | 8 | |||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Primary structure of the Saccharomyces cerevisiae GAL4 gene." Laughon A., Gesteland R.F. Mol. Cell. Biol. 4:260-267(1984) [PubMed: 6366516] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. Hani J.Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [3] | "Genetic evidence that zinc is an essential co-factor in the DNA binding domain of GAL4 protein." Johnston M. Nature 328:353-355(1987) [PubMed: 3299106] [Abstract] Cited for: MUTAGENESIS, ZINC REQUIREMENT. |
| [4] | "An activator target in the RNA polymerase II holoenzyme." Koh S.S., Ansari A.Z., Ptashne M., Young R.A. Mol. Cell 1:895-904(1998) [PubMed: 9660972] [Abstract] Cited for: INTERACTION WITH SRB4. |
| [5] | "Evidence that Gal11 protein is a target of the Gal4 activation domain in the mediator." Jeong C.-J., Yang S.-H., Xie Y., Zhang L., Johnston S.A., Kodadek T. Biochemistry 40:9421-9427(2001) [PubMed: 11478912] [Abstract] Cited for: INTERACTION WITH GAL11. |
| [6] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [7] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-712, MASS SPECTROMETRY. |
| [8] | "DNA recognition by GAL4: structure of a protein-DNA complex." Marmorstein R., Carey M., Ptashne M., Harrison S.C. Nature 356:408-414(1992) [PubMed: 1557122] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 1-65. |
| [9] | "GAL4 transcription factor is not a 'zinc finger' but forms a Zn(II)2Cys6 binuclear cluster." Pan T., Coleman J.E. Proc. Natl. Acad. Sci. U.S.A. 87:2077-2081(1990) [PubMed: 2107541] [Abstract] Cited for: STRUCTURE BY NMR OF ZINC-BINDING REGION. |
| [10] | "Sequential assignments of the 1H NMR resonances of Zn(II)2 and 113Cd(II)2 derivatives of the DNA-binding domain of the GAL4 transcription factor reveal a novel structural motif for specific DNA recognition." Pan T., Coleman J.E. Biochemistry 30:4212-4222(1991) [PubMed: 2021614] [Abstract] Cited for: STRUCTURE BY NMR OF ZINC-BINDING REGION. |
| [11] | "Complete assignment of the 1H NMR spectrum and secondary structure of the DNA binding domain of GAL4." Gadhavi P.L., Raine A.R.C., Alefounder P.R., Laue E.D. FEBS Lett. 276:49-53(1990) [PubMed: 2265711] [Abstract] Cited for: STRUCTURE BY NMR OF ZINC-BINDING REGION. |
| [12] | "Structure of the DNA-binding domain of zinc GAL4." Kraulis P.J., Raine A.R.C., Gadhavi P.L., Laue E.D. Nature 356:448-450(1992) [PubMed: 1557129] [Abstract] Cited for: STRUCTURE BY NMR OF ZINC-BINDING REGION. |
| [13] | "Solution structure of the DNA-binding domain of Cd2-GAL4 from S. cerevisiae." Baleja J.D., Marmorstein R., Harrison S.C., Wagner G. Nature 356:450-453(1992) [PubMed: 1557130] [Abstract] Cited for: STRUCTURE BY NMR OF ZINC-BINDING REGION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| K01486 Genomic DNA. Translation: AAA34626.1. Z67751 Genomic DNA. Translation: CAA91596.1. Z73604 Genomic DNA. Translation: CAA97969.1. | |||||||||||||||||||||||||||||||||||||
| PIR | RGBYG4. A05022. | ||||||||||||||||||||||||||||||||||||
| RefSeq | NP_015076.1. | ||||||||||||||||||||||||||||||||||||
3D structure databases | |||||||||||||||||||||||||||||||||||||
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| ModBase | Search... | ||||||||||||||||||||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||||||||||||||||||||
| DIP | DIP:593N. | ||||||||||||||||||||||||||||||||||||
| STRING | P04386. | ||||||||||||||||||||||||||||||||||||
Proteomic databases | |||||||||||||||||||||||||||||||||||||
| PeptideAtlas | P04386. | ||||||||||||||||||||||||||||||||||||
Genome annotation databases | |||||||||||||||||||||||||||||||||||||
| Ensembl | YPL248C; YPL248C; YPL248C; Saccharomyces cerevisiae. [Genome view] | ||||||||||||||||||||||||||||||||||||
| GeneID | 855828. | ||||||||||||||||||||||||||||||||||||
| GenomeReviews | Gene locus YPL248C in contig U00094_GR. | ||||||||||||||||||||||||||||||||||||
| KEGG | sce:YPL248C. | ||||||||||||||||||||||||||||||||||||
| NMPDR | fig|4932.3.peg.6203. | ||||||||||||||||||||||||||||||||||||
Organism-specific databases | |||||||||||||||||||||||||||||||||||||
| CYGD | YPL248c. | ||||||||||||||||||||||||||||||||||||
| SGD | S000006169. GAL4. | ||||||||||||||||||||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||||||||||||||||||||
| OMA | IYESELT. | ||||||||||||||||||||||||||||||||||||
Gene expression databases | |||||||||||||||||||||||||||||||||||||
| ArrayExpress | P04386. | ||||||||||||||||||||||||||||||||||||
| Genevestigator | P04386. | ||||||||||||||||||||||||||||||||||||
| GermOnline | YPL248C. Saccharomyces cerevisiae. | ||||||||||||||||||||||||||||||||||||
Family and domain databases | |||||||||||||||||||||||||||||||||||||
| InterPro | IPR007219. Fungal_trans. IPR001138. Fungi_Trscrp_N. IPR005600. Gal4_dimer. [Graphical view] | ||||||||||||||||||||||||||||||||||||
| Pfam | PF04082. Fungal_trans. 1 hit. PF03902. Gal4_dimer. 1 hit. PF00172. Zn_clus. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||||||||
| SMART | SM00066. GAL4. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||||||||
| PROSITE | PS00463. ZN2_CY6_FUNGAL_1. 1 hit. PS50048. ZN2_CY6_FUNGAL_2. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||||||||||||||||||||
Other Resources | |||||||||||||||||||||||||||||||||||||
| NextBio | 980383. | ||||||||||||||||||||||||||||||||||||
Entry information
| Entry name | GAL4_YEAST | ||||||||
| Accession | Primary (citable) accession number: P04386 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |

Clusters with


