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P04385 (GAL1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Galactokinase
EC=2.7.1.6
Alternative name(s):
Galactose kinase
Gene names
Name:GAL1
Ordered Locus Names:YBR020W
ORF Names:YBR0302
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length528 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + D-galactose = ADP + alpha-D-galactose 1-phosphate.

Pathway

Carbohydrate metabolism; galactose metabolism.

Sequence similarities

Belongs to the GHMP kinase family. GalK subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.6
Chain2 – 528527Galactokinase
PRO_0000184657

Regions

Nucleotide binding163 – 17311ATP Potential

Amino acid modifications

Modified residue3491Phosphoserine Ref.7

Experimental info

Sequence conflict61S → R AA sequence Ref.6
Sequence conflict1621V → I in AAA34631. Ref.1
Sequence conflict206 – 23934EHYVG…KPQLK → DIMLVLTMAVWIRLPLFAVR KIMLYTLSSNAVE in AAA34631. Ref.1
Sequence conflict2971L → P in AAA34631. Ref.1
Sequence conflict4281A → V in AAA34631. Ref.1
Sequence conflict4831N → NN in AAA34631. Ref.1

Secondary structure

1 ..................................................................... 528
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P04385 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: 1DA31A1915C19107

FASTA52857,944
        10         20         30         40         50         60 
MTKSHSEEVI VPEFNSSAKE LPRPLAEKCP SIIKKFISAY DAKPDFVARS PGRVNLIGEH 

        70         80         90        100        110        120 
IDYCDFSVLP LAIDFDMLCA VKVLNEKNPS ITLINADPKF AQRKFDLPLD GSYVTIDPSV 

       130        140        150        160        170        180 
SDWSNYFKCG LHVAHSFLKK LAPERFASAP LAGLQVFCEG DVPTGSGLSS SAAFICAVAL 

       190        200        210        220        230        240 
AVVKANMGPG YHMSKQNLMR ITVVAEHYVG VNNGGMDQAA SVCGEEDHAL YVEFKPQLKA 

       250        260        270        280        290        300 
TPFKFPQLKN HEISFVIANT LVVSNKFETA PTNYNLRVVE VTTAANVLAA TYGVVLLSGK 

       310        320        330        340        350        360 
EGSSTNKGNL RDFMNVYYAR YHNISTPWNG DIESGIERLT KMLVLVEESL ANKKQGFSVD 

       370        380        390        400        410        420 
DVAQSLNCSR EEFTRDYLTT SPVRFQVLKL YQRAKHVYSE SLRVLKAVKL MTTASFTADE 

       430        440        450        460        470        480 
DFFKQFGALM NESQASCDKL YECSCPEIDK ICSIALSNGS YGSRLTGAGW GGCTVHLVPG 

       490        500        510        520 
GPNGNIEKVK EALANEFYKV KYPKITDAEL ENAIIVSKPA LGSCLYEL

« Hide

References

« Hide 'large scale' references
[1]"Sequence of the Saccharomyces GAL region and its transcription in vivo."
Citron B.A., Donelson J.E.
J. Bacteriol. 158:269-278(1984) [PubMed: 6715281] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Carlsbergensis.
[2]"The complete sequence of a 33 kb fragment on the right arm of chromosome II from Saccharomyces cerevisiae reveals 16 open reading frames, including ten new open reading frames, five previously identified genes and a homologue of the SCO1 gene."
Smits P.H.M., de Haan M., Maat C., Grivell L.A.
Yeast 10:S75-S80(1994) [PubMed: 8091864] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae."
Johnston M., Davis R.W.
Mol. Cell. Biol. 4:1440-1448(1984) [PubMed: 6092912] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-29.
[6]"The NH2-terminal sequences of galactokinase from Escherichia coli and Saccharomyces cerevisiae."
Schlesinger D.H., Schell M.A., Wilson D.B.
FEBS Lett. 83:45-47(1977) [PubMed: 200486] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-12.
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-349, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		K01609 Genomic DNA. Translation: AAA34631.1.
X76078 Genomic DNA. Translation: CAA53677.1.
Z35889 Genomic DNA. Translation: CAA84962.1.
K02115 Genomic DNA. Translation: AAA34621.1.
BK006936 Genomic DNA. Translation: DAA07142.1.
PIRKIBYGG. S45876.
RefSeqNP_009576.1. NM_001178368.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2AJ4X-ray2.40A/B2-527[»]
ProteinModelPortalP04385.
SMRP04385. Positions 9-528.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-2333N.
MINTMINT-551876.
STRINGP04385.

Proteomic databases

PeptideAtlasP04385.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBR020W; YBR020W; YBR020W.
GeneID852308.
KEGGsce:YBR020W.
NMPDRfig|4932.3.peg.271.

Organism-specific databases

SGDS000000224. GAL1.

Phylogenomic databases

eggNOGfuNOG06496.
GeneTreeEFGT00050000006818.
HOGENOMHBG725121.
OMAHVFTEAL.
OrthoDBEOG4GTPNF.

Gene expression databases

ArrayExpressP04385.
GenevestigatorP04385.
GermOnlineYBR020W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR000705. Galactokinase.
IPR019741. Galactokinase_CS.
IPR019539. GalKase_gal-bd.
IPR006204. GHMP_kinase.
IPR013750. GHMP_kinase_C.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR006206. Mevalonate/galactokinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
Gene3DG3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KOK00849.
PfamPF10509. GalKase_gal_bdg. 1 hit.
PF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFPIRSF000530. Galactokinase. 1 hit.
PRINTSPR00473. GALCTOKINASE.
PR00959. MEVGALKINASE.
SUPFAMSSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMsTIGR00131. Gal_kin. 1 hit.
PROSITEPS00106. GALACTOKINASE. 1 hit.
PS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio970984.

Entry information

Entry nameGAL1_YEAST
AccessionPrimary (citable) accession number: P04385
Secondary accession number(s): D6VQ22
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: January 23, 2007
Last modified: December 14, 2011
This is version 121 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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