Reviewed, UniProtKB/Swiss-Prot P04385 (GAL1_YEAST)

Names and origin

Protein names

Recommended name:
    Galactokinase
    EC=2.7.1.6
Alternative name(s):
    Galactose kinase

Gene names

Name:	GAL1
Ordered Locus Names:	YBR020W
ORF Names:	YBR0302

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	528 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + D-galactose = ADP + alpha-D-galactose 1-phosphate.
Pathway	Carbohydrate metabolism; galactose metabolism.
Sequence similarities	Belongs to the GHMP kinase family. GalK subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Galactose metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate phosphorylation Inferred from direct assay. Source: SGD galactose catabolic process via UDP-galactose Inferred from direct assay. Source: SGD galactose transport Inferred from mutant phenotype. Source: SGD positive regulation of transcription by galactose Inferred from genetic interaction. Source: SGD
Cellular component	cytoplasm Inferred from genetic interaction. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW galactokinase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.5

Chain

2 – 528

527

Galactokinase

PRO_0000184657

Regions

Nucleotide binding

163 – 173

11

ATP Potential

Amino acid modifications

Modified residue

349

1

Phosphoserine Ref.6

Experimental info

Sequence conflict

6

1

S → R Ref.4

Sequence conflict

162

1

V → I in AAA34631. Ref.1

Sequence conflict

206 – 239

34

EHYVG…KPQLK → DIMLVLTMAVWIRLPLFAVR KIMLYTLSSNAVE Ref.1

Sequence conflict

297

1

L → P in AAA34631. Ref.1

Sequence conflict

428

1

A → V in AAA34631. Ref.1

Sequence conflict

483

1

N → NN in AAA34631. Ref.1

Secondary structure

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528

Helix Strand Turn

Details...

Helix

23 – 40

18

Beta strand

45 – 57

13

Helix

62 – 64

3

Beta strand

68 – 84

17

Beta strand

90 – 97

8

Beta strand

103 – 106

4

Helix

123 – 141

19

Helix

143 – 146

4

Beta strand

147 – 149

3

Beta strand

153 – 160

8

Beta strand

166 – 168

3

Helix

170 – 187

18

Helix

195 – 202

8

Helix

203 – 209

7

Helix

216 – 223

8

Beta strand

229 – 233

5

Beta strand

235 – 237

3

Beta strand

239 – 243

5

Beta strand

252 – 259

8

Turn

266 – 273

8

Helix

274 – 291

18

Helix

310 – 322

13

Helix

332 – 350

19

Helix

351 – 353

3

Helix

359 – 366

8

Helix

370 – 378

9

Beta strand

379 – 381

3

Helix

390 – 412

23

Helix

419 – 439

21

Helix

446 – 457

12

Beta strand

461 – 465

5

Beta strand

470 – 481

12

Helix

486 – 496

11

Helix

498 – 501

4

Helix

507 – 513

7

Beta strand

514 – 517

4

Beta strand

523 – 527

5

Sequences

	Sequence		Length	Mass (Da)
	P04385-1 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 1DA31A1915C19107	FASTA	528	57,944
	10 20 30 40 50 60 MTKSHSEEVI VPEFNSSAKE LPRPLAEKCP SIIKKFISAY DAKPDFVARS PGRVNLIGEH 70 80 90 100 110 120 IDYCDFSVLP LAIDFDMLCA VKVLNEKNPS ITLINADPKF AQRKFDLPLD GSYVTIDPSV 130 140 150 160 170 180 SDWSNYFKCG LHVAHSFLKK LAPERFASAP LAGLQVFCEG DVPTGSGLSS SAAFICAVAL 190 200 210 220 230 240 AVVKANMGPG YHMSKQNLMR ITVVAEHYVG VNNGGMDQAA SVCGEEDHAL YVEFKPQLKA 250 260 270 280 290 300 TPFKFPQLKN HEISFVIANT LVVSNKFETA PTNYNLRVVE VTTAANVLAA TYGVVLLSGK 310 320 330 340 350 360 EGSSTNKGNL RDFMNVYYAR YHNISTPWNG DIESGIERLT KMLVLVEESL ANKKQGFSVD 370 380 390 400 410 420 DVAQSLNCSR EEFTRDYLTT SPVRFQVLKL YQRAKHVYSE SLRVLKAVKL MTTASFTADE 430 440 450 460 470 480 DFFKQFGALM NESQASCDKL YECSCPEIDK ICSIALSNGS YGSRLTGAGW GGCTVHLVPG 490 500 510 520 GPNGNIEKVK EALANEFYKV KYPKITDAEL ENAIIVSKPA LGSCLYEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of the Saccharomyces GAL region and its transcription in vivo." Citron B.A., Donelson J.E. J. Bacteriol. 158:269-278(1984) [PubMed: 6715281] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Carlsbergensis.
[2]	"The complete sequence of a 33 kb fragment on the right arm of chromosome II from Saccharomyces cerevisiae reveals 16 open reading frames, including ten new open reading frames, five previously identified genes and a homologue of the SCO1 gene." Smits P.H.M., de Haan M., Maat C., Grivell L.A. Yeast 10:S75-S80(1994) [PubMed: 8091864] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae." Johnston M., Davis R.W. Mol. Cell. Biol. 4:1440-1448(1984) [PubMed: 6092912] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-29.
[5]	"The NH2-terminal sequences of galactokinase from Escherichia coli and Saccharomyces cerevisiae." Schlesinger D.H., Schell M.A., Wilson D.B. FEBS Lett. 83:45-47(1977) [PubMed: 200486] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-12.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-349, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

K01609 Genomic DNA. Translation: AAA34631.1.
X76078 Genomic DNA. Translation: CAA53677.1.
Z35889 Genomic DNA. Translation: CAA84962.1.
K02115 Genomic DNA. Translation: AAA34621.1.

PIR

KIBYGG. S45876.

RefSeq

NP_009576.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2AJ4	X-ray	2.40	A/B	2-527	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:2333N.

IntAct

P04385. 2 interactions.

Proteomic databases

PeptideAtlas

P04385.

PRIDE

P04385.

Genome annotation databases

Ensembl

YBR020W. Saccharomyces cerevisiae. [Contig view]

GeneID

852308.

GenomeReviews

Gene locus YBR020W in contig Y13134_GR.

KEGG

sce:YBR020W.

NMPDR

fig|4932.3.peg.271.

Organism-specific databases

CYGD

YBR020w.

SGD

S000000224. GAL1.

Yeast-GFP

Search...

Phylogenomic databases

HOGENOM

P04385.

OMA

P04385. PMAITAD.

Enzyme and pathway databases

BRENDA

2.7.1.6. 250.

Gene expression databases

ArrayExpress

P04385.

GermOnline

YBR020W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR000705. Galactokinase.
IPR019741. Galactokinase_CS.
IPR019539. GalKase_gal_bd.
IPR006204. GHMP_kinase.
IPR013750. GHMP_kinase_C.
IPR006203. GHMP_knse_ATP_bd_CS.
IPR006206. Mev_galkinase.
IPR014721. Ribosomal_S5_D2-type_fold.
[Graphical view]

Gene3D

G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.

Pfam

PF10509. GalKase_gal_bdg. 1 hit.
PF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]

PIRSF

PIRSF000530. Galactokinase. 1 hit.

PRINTS

PR00473. GALCTOKINASE.
PR00959. MEVGALKINASE.

TIGRFAMs

TIGR00131. gal_kin. 1 hit.

PROSITE

PS00106. GALACTOKINASE. 1 hit.
PS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

970984.

Entry information

Entry name

GAL1_YEAST

Accession

Primary (citable) accession number: P04385

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 1987
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 95 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)