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Protein

Galactokinase

Gene

GAL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + alpha-D-galactose = ADP + alpha-D-galactose 1-phosphate.

Pathwayi: galactose metabolism

This protein is involved in the pathway galactose metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway galactose metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei53 – 531Transition state stabilizerBy similarity
Active sitei217 – 2171Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi163 – 17311ATPSequence analysisAdd
BLAST

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • galactokinase activity Source: SGD

GO - Biological processi

  • carbohydrate phosphorylation Source: SGD
  • galactose catabolic process via UDP-galactose Source: SGD
  • positive regulation of transcription by galactose Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Galactose metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YBR020W-MONOMER.
ReactomeiR-SCE-70370. Galactose catabolism.
UniPathwayiUPA00214.

Names & Taxonomyi

Protein namesi
Recommended name:
Galactokinase (EC:2.7.1.6)
Alternative name(s):
Galactose kinase
Gene namesi
Name:GAL1
Ordered Locus Names:YBR020W
ORF Names:YBR0302
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR020W.
SGDiS000000224. GAL1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 528527GalactokinasePRO_0000184657Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei381 – 3811PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PeptideAtlasiP04385.

PTM databases

iPTMnetiP04385.

Interactioni

Protein-protein interaction databases

BioGridi32723. 31 interactions.
DIPiDIP-2333N.
MINTiMINT-551876.

Structurei

Secondary structure

1
528
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi23 – 4018Combined sources
Beta strandi45 – 5713Combined sources
Helixi62 – 643Combined sources
Beta strandi68 – 8417Combined sources
Beta strandi90 – 978Combined sources
Beta strandi103 – 1064Combined sources
Helixi123 – 14119Combined sources
Helixi143 – 1464Combined sources
Beta strandi147 – 1493Combined sources
Beta strandi153 – 1608Combined sources
Beta strandi166 – 1683Combined sources
Helixi170 – 18718Combined sources
Helixi195 – 2028Combined sources
Helixi203 – 2097Combined sources
Helixi216 – 2238Combined sources
Beta strandi229 – 2335Combined sources
Beta strandi235 – 2373Combined sources
Beta strandi239 – 2435Combined sources
Beta strandi252 – 2598Combined sources
Turni266 – 2738Combined sources
Helixi274 – 29118Combined sources
Helixi310 – 32213Combined sources
Helixi332 – 35019Combined sources
Helixi351 – 3533Combined sources
Helixi359 – 3668Combined sources
Helixi370 – 3789Combined sources
Beta strandi379 – 3813Combined sources
Helixi390 – 41223Combined sources
Helixi419 – 43921Combined sources
Helixi446 – 45712Combined sources
Beta strandi461 – 4655Combined sources
Beta strandi470 – 48112Combined sources
Helixi486 – 49611Combined sources
Helixi498 – 5014Combined sources
Helixi507 – 5137Combined sources
Beta strandi514 – 5174Combined sources
Beta strandi523 – 5275Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AJ4X-ray2.40A/B2-528[»]
ProteinModelPortaliP04385.
SMRiP04385. Positions 9-528.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04385.

Family & Domainsi

Sequence similaritiesi

Belongs to the GHMP kinase family. GalK subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00530000063433.
HOGENOMiHOG000241099.
InParanoidiP04385.
KOiK00849.
OMAiEICKCLG.
OrthoDBiEOG7FZ075.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 2 hits.
InterProiIPR000705. Galactokinase.
IPR019741. Galactokinase_CS.
IPR019539. GalKase_gal-bd.
IPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR006206. Mevalonate/galactokinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10457. PTHR10457. 2 hits.
PfamiPF10509. GalKase_gal_bdg. 1 hit.
PF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000530. Galactokinase. 1 hit.
PRINTSiPR00473. GALCTOKINASE.
PR00959. MEVGALKINASE.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 2 hits.
TIGRFAMsiTIGR00131. gal_kin. 1 hit.
PROSITEiPS00106. GALACTOKINASE. 1 hit.
PS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04385-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKSHSEEVI VPEFNSSAKE LPRPLAEKCP SIIKKFISAY DAKPDFVARS
60 70 80 90 100
PGRVNLIGEH IDYCDFSVLP LAIDFDMLCA VKVLNEKNPS ITLINADPKF
110 120 130 140 150
AQRKFDLPLD GSYVTIDPSV SDWSNYFKCG LHVAHSFLKK LAPERFASAP
160 170 180 190 200
LAGLQVFCEG DVPTGSGLSS SAAFICAVAL AVVKANMGPG YHMSKQNLMR
210 220 230 240 250
ITVVAEHYVG VNNGGMDQAA SVCGEEDHAL YVEFKPQLKA TPFKFPQLKN
260 270 280 290 300
HEISFVIANT LVVSNKFETA PTNYNLRVVE VTTAANVLAA TYGVVLLSGK
310 320 330 340 350
EGSSTNKGNL RDFMNVYYAR YHNISTPWNG DIESGIERLT KMLVLVEESL
360 370 380 390 400
ANKKQGFSVD DVAQSLNCSR EEFTRDYLTT SPVRFQVLKL YQRAKHVYSE
410 420 430 440 450
SLRVLKAVKL MTTASFTADE DFFKQFGALM NESQASCDKL YECSCPEIDK
460 470 480 490 500
ICSIALSNGS YGSRLTGAGW GGCTVHLVPG GPNGNIEKVK EALANEFYKV
510 520
KYPKITDAEL ENAIIVSKPA LGSCLYEL
Length:528
Mass (Da):57,944
Last modified:January 23, 2007 - v4
Checksum:i1DA31A1915C19107
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 61S → R AA sequence (PubMed:200486).Curated
Sequence conflicti162 – 1621V → I in AAA34631 (PubMed:6715281).Curated
Sequence conflicti206 – 23934EHYVG…KPQLK → DIMLVLTMAVWIRLPLFAVR KIMLYTLSSNAVE in AAA34631 (PubMed:6715281).CuratedAdd
BLAST
Sequence conflicti297 – 2971L → P in AAA34631 (PubMed:6715281).Curated
Sequence conflicti428 – 4281A → V in AAA34631 (PubMed:6715281).Curated
Sequence conflicti483 – 4831N → NN in AAA34631 (PubMed:6715281).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01609 Genomic DNA. Translation: AAA34631.1.
X76078 Genomic DNA. Translation: CAA53677.1.
Z35889 Genomic DNA. Translation: CAA84962.1.
K02115 Genomic DNA. Translation: AAA34621.1.
BK006936 Genomic DNA. Translation: DAA07142.1.
PIRiS45876. KIBYGG.
RefSeqiNP_009576.1. NM_001178368.1.

Genome annotation databases

EnsemblFungiiYBR020W; YBR020W; YBR020W.
GeneIDi852308.
KEGGisce:YBR020W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01609 Genomic DNA. Translation: AAA34631.1.
X76078 Genomic DNA. Translation: CAA53677.1.
Z35889 Genomic DNA. Translation: CAA84962.1.
K02115 Genomic DNA. Translation: AAA34621.1.
BK006936 Genomic DNA. Translation: DAA07142.1.
PIRiS45876. KIBYGG.
RefSeqiNP_009576.1. NM_001178368.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AJ4X-ray2.40A/B2-528[»]
ProteinModelPortaliP04385.
SMRiP04385. Positions 9-528.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32723. 31 interactions.
DIPiDIP-2333N.
MINTiMINT-551876.

PTM databases

iPTMnetiP04385.

Proteomic databases

PeptideAtlasiP04385.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR020W; YBR020W; YBR020W.
GeneIDi852308.
KEGGisce:YBR020W.

Organism-specific databases

EuPathDBiFungiDB:YBR020W.
SGDiS000000224. GAL1.

Phylogenomic databases

GeneTreeiENSGT00530000063433.
HOGENOMiHOG000241099.
InParanoidiP04385.
KOiK00849.
OMAiEICKCLG.
OrthoDBiEOG7FZ075.

Enzyme and pathway databases

UniPathwayiUPA00214.
BioCyciYEAST:YBR020W-MONOMER.
ReactomeiR-SCE-70370. Galactose catabolism.

Miscellaneous databases

EvolutionaryTraceiP04385.
NextBioi970984.
PROiP04385.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 2 hits.
InterProiIPR000705. Galactokinase.
IPR019741. Galactokinase_CS.
IPR019539. GalKase_gal-bd.
IPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR006206. Mevalonate/galactokinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10457. PTHR10457. 2 hits.
PfamiPF10509. GalKase_gal_bdg. 1 hit.
PF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000530. Galactokinase. 1 hit.
PRINTSiPR00473. GALCTOKINASE.
PR00959. MEVGALKINASE.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 2 hits.
TIGRFAMsiTIGR00131. gal_kin. 1 hit.
PROSITEiPS00106. GALACTOKINASE. 1 hit.
PS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of the Saccharomyces GAL region and its transcription in vivo."
    Citron B.A., Donelson J.E.
    J. Bacteriol. 158:269-278(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Carlsbergensis.
  2. "The complete sequence of a 33 kb fragment on the right arm of chromosome II from Saccharomyces cerevisiae reveals 16 open reading frames, including ten new open reading frames, five previously identified genes and a homologue of the SCO1 gene."
    Smits P.H.M., de Haan M., Maat C., Grivell L.A.
    Yeast 10:S75-S80(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae."
    Johnston M., Davis R.W.
    Mol. Cell. Biol. 4:1440-1448(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-29.
  6. "The NH2-terminal sequences of galactokinase from Escherichia coli and Saccharomyces cerevisiae."
    Schlesinger D.H., Schell M.A., Wilson D.B.
    FEBS Lett. 83:45-47(1977) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-12.
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-381, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGAL1_YEAST
AccessioniPrimary (citable) accession number: P04385
Secondary accession number(s): D6VQ22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 155 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.