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Protein

Galactokinase

Gene

GAL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + alpha-D-galactose = ADP + alpha-D-galactose 1-phosphate.

Pathwayi: galactose metabolism

This protein is involved in the pathway galactose metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway galactose metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei53Transition state stabilizerBy similarity1
Active sitei217Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi163 – 173ATPSequence analysisAdd BLAST11

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • galactokinase activity Source: SGD

GO - Biological processi

  • carbohydrate phosphorylation Source: SGD
  • galactose catabolic process via UDP-galactose Source: SGD
  • positive regulation of transcription by galactose Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Galactose metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YBR020W-MONOMER.
ReactomeiR-SCE-70370. Galactose catabolism.
UniPathwayiUPA00214.

Names & Taxonomyi

Protein namesi
Recommended name:
Galactokinase (EC:2.7.1.6)
Alternative name(s):
Galactose kinase
Gene namesi
Name:GAL1
Ordered Locus Names:YBR020W
ORF Names:YBR0302
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR020W.
SGDiS000000224. GAL1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001846572 – 528GalactokinaseAdd BLAST527

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei381PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP04385.

PTM databases

iPTMnetiP04385.

Interactioni

Protein-protein interaction databases

BioGridi32723. 31 interactors.
DIPiDIP-2333N.
MINTiMINT-551876.

Structurei

Secondary structure

1528
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi23 – 40Combined sources18
Beta strandi45 – 57Combined sources13
Helixi62 – 64Combined sources3
Beta strandi68 – 84Combined sources17
Beta strandi90 – 97Combined sources8
Beta strandi103 – 106Combined sources4
Helixi123 – 141Combined sources19
Helixi143 – 146Combined sources4
Beta strandi147 – 149Combined sources3
Beta strandi153 – 160Combined sources8
Beta strandi166 – 168Combined sources3
Helixi170 – 187Combined sources18
Helixi195 – 202Combined sources8
Helixi203 – 209Combined sources7
Helixi216 – 223Combined sources8
Beta strandi229 – 233Combined sources5
Beta strandi235 – 237Combined sources3
Beta strandi239 – 243Combined sources5
Beta strandi252 – 259Combined sources8
Turni266 – 273Combined sources8
Helixi274 – 291Combined sources18
Helixi310 – 322Combined sources13
Helixi332 – 350Combined sources19
Helixi351 – 353Combined sources3
Helixi359 – 366Combined sources8
Helixi370 – 378Combined sources9
Beta strandi379 – 381Combined sources3
Helixi390 – 412Combined sources23
Helixi419 – 439Combined sources21
Helixi446 – 457Combined sources12
Beta strandi461 – 465Combined sources5
Beta strandi470 – 481Combined sources12
Helixi486 – 496Combined sources11
Helixi498 – 501Combined sources4
Helixi507 – 513Combined sources7
Beta strandi514 – 517Combined sources4
Beta strandi523 – 527Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AJ4X-ray2.40A/B2-528[»]
ProteinModelPortaliP04385.
SMRiP04385.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04385.

Family & Domainsi

Sequence similaritiesi

Belongs to the GHMP kinase family. GalK subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00530000063433.
HOGENOMiHOG000241099.
InParanoidiP04385.
KOiK00849.
OMAiDMLFHDT.
OrthoDBiEOG092C193C.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 2 hits.
InterProiIPR000705. Galactokinase.
IPR019741. Galactokinase_CS.
IPR019539. GalKase_gal-bd.
IPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR006206. Mevalonate/galactokinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10457. PTHR10457. 2 hits.
PfamiPF10509. GalKase_gal_bdg. 1 hit.
PF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000530. Galactokinase. 1 hit.
PRINTSiPR00473. GALCTOKINASE.
PR00959. MEVGALKINASE.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 2 hits.
TIGRFAMsiTIGR00131. gal_kin. 1 hit.
PROSITEiPS00106. GALACTOKINASE. 1 hit.
PS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04385-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKSHSEEVI VPEFNSSAKE LPRPLAEKCP SIIKKFISAY DAKPDFVARS
60 70 80 90 100
PGRVNLIGEH IDYCDFSVLP LAIDFDMLCA VKVLNEKNPS ITLINADPKF
110 120 130 140 150
AQRKFDLPLD GSYVTIDPSV SDWSNYFKCG LHVAHSFLKK LAPERFASAP
160 170 180 190 200
LAGLQVFCEG DVPTGSGLSS SAAFICAVAL AVVKANMGPG YHMSKQNLMR
210 220 230 240 250
ITVVAEHYVG VNNGGMDQAA SVCGEEDHAL YVEFKPQLKA TPFKFPQLKN
260 270 280 290 300
HEISFVIANT LVVSNKFETA PTNYNLRVVE VTTAANVLAA TYGVVLLSGK
310 320 330 340 350
EGSSTNKGNL RDFMNVYYAR YHNISTPWNG DIESGIERLT KMLVLVEESL
360 370 380 390 400
ANKKQGFSVD DVAQSLNCSR EEFTRDYLTT SPVRFQVLKL YQRAKHVYSE
410 420 430 440 450
SLRVLKAVKL MTTASFTADE DFFKQFGALM NESQASCDKL YECSCPEIDK
460 470 480 490 500
ICSIALSNGS YGSRLTGAGW GGCTVHLVPG GPNGNIEKVK EALANEFYKV
510 520
KYPKITDAEL ENAIIVSKPA LGSCLYEL
Length:528
Mass (Da):57,944
Last modified:January 23, 2007 - v4
Checksum:i1DA31A1915C19107
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6S → R AA sequence (PubMed:200486).Curated1
Sequence conflicti162V → I in AAA34631 (PubMed:6715281).Curated1
Sequence conflicti206 – 239EHYVG…KPQLK → DIMLVLTMAVWIRLPLFAVR KIMLYTLSSNAVE in AAA34631 (PubMed:6715281).CuratedAdd BLAST34
Sequence conflicti297L → P in AAA34631 (PubMed:6715281).Curated1
Sequence conflicti428A → V in AAA34631 (PubMed:6715281).Curated1
Sequence conflicti483N → NN in AAA34631 (PubMed:6715281).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01609 Genomic DNA. Translation: AAA34631.1.
X76078 Genomic DNA. Translation: CAA53677.1.
Z35889 Genomic DNA. Translation: CAA84962.1.
K02115 Genomic DNA. Translation: AAA34621.1.
BK006936 Genomic DNA. Translation: DAA07142.1.
PIRiS45876. KIBYGG.
RefSeqiNP_009576.1. NM_001178368.1.

Genome annotation databases

EnsemblFungiiYBR020W; YBR020W; YBR020W.
GeneIDi852308.
KEGGisce:YBR020W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01609 Genomic DNA. Translation: AAA34631.1.
X76078 Genomic DNA. Translation: CAA53677.1.
Z35889 Genomic DNA. Translation: CAA84962.1.
K02115 Genomic DNA. Translation: AAA34621.1.
BK006936 Genomic DNA. Translation: DAA07142.1.
PIRiS45876. KIBYGG.
RefSeqiNP_009576.1. NM_001178368.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AJ4X-ray2.40A/B2-528[»]
ProteinModelPortaliP04385.
SMRiP04385.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32723. 31 interactors.
DIPiDIP-2333N.
MINTiMINT-551876.

PTM databases

iPTMnetiP04385.

Proteomic databases

PRIDEiP04385.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR020W; YBR020W; YBR020W.
GeneIDi852308.
KEGGisce:YBR020W.

Organism-specific databases

EuPathDBiFungiDB:YBR020W.
SGDiS000000224. GAL1.

Phylogenomic databases

GeneTreeiENSGT00530000063433.
HOGENOMiHOG000241099.
InParanoidiP04385.
KOiK00849.
OMAiDMLFHDT.
OrthoDBiEOG092C193C.

Enzyme and pathway databases

UniPathwayiUPA00214.
BioCyciYEAST:YBR020W-MONOMER.
ReactomeiR-SCE-70370. Galactose catabolism.

Miscellaneous databases

EvolutionaryTraceiP04385.
PROiP04385.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 2 hits.
InterProiIPR000705. Galactokinase.
IPR019741. Galactokinase_CS.
IPR019539. GalKase_gal-bd.
IPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR006206. Mevalonate/galactokinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10457. PTHR10457. 2 hits.
PfamiPF10509. GalKase_gal_bdg. 1 hit.
PF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000530. Galactokinase. 1 hit.
PRINTSiPR00473. GALCTOKINASE.
PR00959. MEVGALKINASE.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 2 hits.
TIGRFAMsiTIGR00131. gal_kin. 1 hit.
PROSITEiPS00106. GALACTOKINASE. 1 hit.
PS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGAL1_YEAST
AccessioniPrimary (citable) accession number: P04385
Secondary accession number(s): D6VQ22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 160 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.