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P04382

- DYR_BPT4

UniProt

P04382 - DYR_BPT4

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Protein

Dihydrofolate reductase

Gene

frd

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei7 – 71NADP; via amide nitrogen and carbonyl oxygen

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi22 – 276NADP
Nucleotide bindingi52 – 554NADP
Nucleotide bindingi73 – 775NADP

GO - Molecular functioni

  1. dihydrofolate reductase activity Source: UniProtKB-EC
  2. NADP binding Source: InterPro

GO - Biological processi

  1. glycine biosynthetic process Source: InterPro
  2. nucleotide biosynthetic process Source: InterPro
  3. one-carbon metabolic process Source: UniProtKB-KW
  4. response to antibiotic Source: UniProtKB-KW
  5. response to methotrexate Source: UniProtKB-KW
  6. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Antibiotic resistance, Methotrexate resistance, One-carbon metabolism, Trimethoprim resistance

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase (EC:1.5.1.3)
Gene namesi
Name:frd
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostiEscherichia coli [TaxID: 562]
ProteomesiUP000009087: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 193193Dihydrofolate reductasePRO_0000186434Add
BLAST

Structurei

Secondary structure

1
193
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 1211
Beta strandi18 – 247
Helixi34 – 4411
Beta strandi47 – 526
Helixi53 – 575
Beta strandi68 – 714
Beta strandi88 – 914
Helixi93 – 1008
Beta strandi105 – 1084
Beta strandi115 – 1184
Beta strandi124 – 1285
Helixi130 – 1367
Helixi137 – 1393
Beta strandi141 – 15212
Beta strandi157 – 1604
Helixi162 – 1698
Beta strandi173 – 18210
Beta strandi185 – 1928

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JUVX-ray1.70A1-193[»]
ProteinModelPortaliP04382.
SMRiP04382. Positions 1-193.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04382.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 193193DHFRPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated
Contains 1 DHFR (dihydrofolate reductase) domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04382-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIKLVFRYSP TKTVDGFNEL AFGLGDGLPW GRVKKDLQNF KARTEGTIMI
60 70 80 90 100
MGAKTFQSLP TLLPGRSHIV VCDLARDYPV TKDGDLAHFY ITWEQYITYI
110 120 130 140 150
SGGEIQVSSP NAPFETMLDQ NSKVSVIGGP ALLYAALPYA DEVVVSRIVK
160 170 180 190
RHRVNSTVQL DASFLDDISK REMVETHWYK IDEVTTLTES VYK
Length:193
Mass (Da):21,713
Last modified:March 20, 1987 - v1
Checksum:i64E033916E628178
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K01804 Genomic DNA. Translation: AAA32491.1.
AF158101 Genomic DNA. Translation: AAD42577.1.
M12742 Genomic DNA. Translation: AAC12815.1.
PIRiA00396. RDBPT4.
RefSeqiNP_049850.1. NC_000866.4.

Genome annotation databases

GeneIDi1258671.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K01804 Genomic DNA. Translation: AAA32491.1 .
AF158101 Genomic DNA. Translation: AAD42577.1 .
M12742 Genomic DNA. Translation: AAC12815.1 .
PIRi A00396. RDBPT4.
RefSeqi NP_049850.1. NC_000866.4.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1JUV X-ray 1.70 A 1-193 [» ]
ProteinModelPortali P04382.
SMRi P04382. Positions 1-193.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1258671.

Enzyme and pathway databases

UniPathwayi UPA00077 ; UER00158 .

Miscellaneous databases

EvolutionaryTracei P04382.

Family and domain databases

Gene3Di 3.40.430.10. 1 hit.
InterProi IPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view ]
Pfami PF00186. DHFR_1. 1 hit.
[Graphical view ]
PRINTSi PR00070. DHFR.
SUPFAMi SSF53597. SSF53597. 1 hit.
PROSITEi PS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence reveals overlap between T4 phage genes encoding dihydrofolate reductase and thymidylate synthase."
    Purohit S., Mathews C.K.
    J. Biol. Chem. 259:6261-6266(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Characterization of the intron in the phage T4 thymidylate synthase gene and evidence for its self-excision from the primary transcript."
    Chu F.K., Maley G.F., West D.K., Belfort M., Maley F.
    Cell 45:157-166(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 123-193.
    Strain: ALC4.

Entry informationi

Entry nameiDYR_BPT4
AccessioniPrimary (citable) accession number: P04382
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: March 20, 1987
Last modified: October 1, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3