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P04382 (DYR_BPT4) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dihydrofolate reductase

EC=1.5.1.3
Gene names
Name:frd
OrganismEnterobacteria phage T4 (Bacteriophage T4) [Reference proteome]
Taxonomic identifier10665 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostEscherichia coli [TaxID: 562]

Protein attributes

Sequence length193 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.

Catalytic activity

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.

Sequence similarities

Belongs to the dihydrofolate reductase family.

Contains 1 DHFR (dihydrofolate reductase) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 193193Dihydrofolate reductase
PRO_0000186434

Regions

Domain1 – 193193DHFR
Nucleotide binding22 – 276NADP
Nucleotide binding52 – 554NADP
Nucleotide binding73 – 775NADP

Sites

Binding site71NADP; via amide nitrogen and carbonyl oxygen

Secondary structure

................................... 193
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P04382 [UniParc].

Last modified March 20, 1987. Version 1.
Checksum: 64E033916E628178

FASTA19321,713
        10         20         30         40         50         60 
MIKLVFRYSP TKTVDGFNEL AFGLGDGLPW GRVKKDLQNF KARTEGTIMI MGAKTFQSLP 

        70         80         90        100        110        120 
TLLPGRSHIV VCDLARDYPV TKDGDLAHFY ITWEQYITYI SGGEIQVSSP NAPFETMLDQ 

       130        140        150        160        170        180 
NSKVSVIGGP ALLYAALPYA DEVVVSRIVK RHRVNSTVQL DASFLDDISK REMVETHWYK 

       190 
IDEVTTLTES VYK 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence reveals overlap between T4 phage genes encoding dihydrofolate reductase and thymidylate synthase."
Purohit S., Mathews C.K.
J. Biol. Chem. 259:6261-6266(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Bacteriophage T4 genome."
Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.
Microbiol. Mol. Biol. Rev. 67:86-156(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Characterization of the intron in the phage T4 thymidylate synthase gene and evidence for its self-excision from the primary transcript."
Chu F.K., Maley G.F., West D.K., Belfort M., Maley F.
Cell 45:157-166(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 123-193.
Strain: ALC4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
K01804 Genomic DNA. Translation: AAA32491.1.
AF158101 Genomic DNA. Translation: AAD42577.1.
M12742 Genomic DNA. Translation: AAC12815.1.
PIRRDBPT4. A00396.
RefSeqNP_049850.1. NC_000866.4.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1JUVX-ray1.70A1-193[»]
ProteinModelPortalP04382.
SMRP04382. Positions 1-193.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1258671.

Phylogenomic databases

ProtClustDBCLSP2343408.

Enzyme and pathway databases

UniPathwayUPA00077; UER00158.

Family and domain databases

Gene3D3.40.430.10. 1 hit.
InterProIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamPF00186. DHFR_1. 1 hit.
[Graphical view]
PRINTSPR00070. DHFR.
SUPFAMSSF53597. SSF53597. 1 hit.
PROSITEPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP04382.

Entry information

Entry nameDYR_BPT4
AccessionPrimary (citable) accession number: P04382
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: March 20, 1987
Last modified: October 16, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways