Reviewed, UniProtKB/Swiss-Prot P04382 (DYR_BPT4)

Names and origin

Protein names

Recommended name:
    Dihydrofolate reductase
    EC=1.5.1.3

Gene names

Name:

frd

Organism

Enterobacteria phage T4 (Bacteriophage T4)

Taxonomic identifier

10665 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Myoviridae › T4-like viruses

Virus host

Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; tetrahydrofolate from dihydrofolate: step 1/1.
Miscellaneous	The reaction catalyzed by this enzyme represents an essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.
Sequence similarities	Belongs to the dihydrofolate reductase family. Contains 1 DHFR (dihydrofolate reductase) domain.

Ontologies

Keywords
Biological process	Antibiotic resistance Methotrexate resistance One-carbon metabolism Trimethoprim resistance
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	glycine biosynthetic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon compound metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW response to methotrexate Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro dihydrofolate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 193

193

Dihydrofolate reductase

PRO_0000186434

Regions

Domain

1 – 193

193

DHFR

Secondary structure

1

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193

Helix Strand Turn

Details...

Beta strand

2 – 12

11

Beta strand

18 – 24

7

Helix

34 – 44

11

Beta strand

47 – 52

6

Helix

53 – 57

5

Beta strand

68 – 71

4

Beta strand

88 – 91

4

Helix

93 – 100

8

Beta strand

105 – 108

4

Beta strand

115 – 118

4

Beta strand

124 – 128

5

Helix

130 – 136

7

Helix

137 – 139

3

Beta strand

141 – 152

12

Beta strand

157 – 160

4

Helix

162 – 169

8

Beta strand

173 – 182

10

Beta strand

185 – 192

8

Sequences

	Sequence		Length	Mass (Da)
	P04382-1 [UniParc]. Last modified March 20, 1987. Version 1. Checksum: 64E033916E628178	FASTA	193	21,713
	10 20 30 40 50 60 MIKLVFRYSP TKTVDGFNEL AFGLGDGLPW GRVKKDLQNF KARTEGTIMI MGAKTFQSLP 70 80 90 100 110 120 TLLPGRSHIV VCDLARDYPV TKDGDLAHFY ITWEQYITYI SGGEIQVSSP NAPFETMLDQ 130 140 150 160 170 180 NSKVSVIGGP ALLYAALPYA DEVVVSRIVK RHRVNSTVQL DASFLDDISK REMVETHWYK 190 IDEVTTLTES VYK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence reveals overlap between T4 phage genes encoding dihydrofolate reductase and thymidylate synthase." Purohit S., Mathews C.K. J. Biol. Chem. 259:6261-6266(1984) [PubMed: 6327673] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Bacteriophage T4 genome." Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W. Microbiol. Mol. Biol. Rev. 67:86-156(2003) [PubMed: 12626685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Characterization of the intron in the phage T4 thymidylate synthase gene and evidence for its self-excision from the primary transcript." Chu F.K., Maley G.F., West D.K., Belfort M., Maley F. Cell 45:157-166(1986) [PubMed: 3698096] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 123-193. Strain: ALC4.

Cross-references

Sequence databases

EMBLGenBankDDBJ

K01804 Genomic DNA. Translation: AAA32491.1.
AF158101 Genomic DNA. Translation: AAD42577.1.
M12742 Genomic DNA. Translation: AAC12815.1.

PIR

RDBPT4. A00396.

RefSeq

NP_049850.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JUV	X-ray	1.70	A	1-193	[»]

ModBase

Search...

Genome annotation databases

GeneID

1258671.

GenomeReviews

Gene locus T4p240 in contig AF158101_GR.

Enzyme and pathway databases

BRENDA

1.5.1.3. 1108.

Family and domain databases

InterPro

IPR012259. DHFR.
IPR001796. DHFR_reg.
IPR017925. Dihydrofolate_reductase_CS.
[Graphical view]

PANTHER

PTHR11549:SF1. DHFR. 1 hit.

Pfam

PF00186. DHFR_1. 1 hit.
[Graphical view]

PRINTS

PR00070. DHFR.

PROSITE

PS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

DYR_BPT4

Accession

Primary (citable) accession number: P04382

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 1987
Last sequence update:	March 20, 1987
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)