P04377 (AZUP_ALCFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Pseudoazurin Alternative name(s): Blue copper protein Cupredoxin
Organism	Alcaligenes faecalis
Taxonomic identifier	511 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Alcaligenes

Protein attributes

Sequence length	146 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This soluble electron transfer copper protein is required for the inactivation of copper-containing nitrite reductase in the presence of oxygen. Serves as a direct electron donor to the nitrite reductase.
Cofactor	Binds 1 copper ion per subunit.
Subcellular location	Periplasm.
Induction	Under anaerobic growth conditions and by nitrite.
Sequence similarities	Contains 1 plastocyanin-like domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Periplasm
Domain	Signal
Ligand	Copper Metal-binding
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	copper ion binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 23

23

Ref.2

Chain

24 – 146

123

Pseudoazurin

PRO_0000002849

Regions

Domain

28 – 116

89

Plastocyanin-like

Sites

Metal binding

63

1

Copper Ref.4

Metal binding

101

1

Copper Ref.4

Metal binding

104

1

Copper Ref.4

Metal binding

109

1

Copper Ref.4

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

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.

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146

Helix Strand Turn

Details...

Beta strand

25 – 34

10

Beta strand

37 – 48

12

Beta strand

53 – 62

10

Beta strand

87 – 90

4

Beta strand

95 – 100

6

Turn

102 – 104

3

Helix

105 – 107

3

Beta strand

110 – 118

9

Helix

122 – 127

6

Helix

132 – 142

11

Sequences

	Sequence		Length	Mass (Da)
	P04377 [UniParc]. Last modified July 1, 1989. Version 2. Checksum: E4093B9A289F947F	FASTA	146	15,647
	10 20 30 40 50 60 MRNIAIKFAA AGILAMLAAP ALAENIEVHM LNKGAEGAMV FEPAYIKANP GDTVTFIPVD 70 80 90 100 110 120 KGHNVESIKD MIPEGAEKFK SKINENYVLT VTQPGAYLVK CTPHYAMGMI ALIAVGDSPA 130 140 NLDQIVSAKK PKIVQERLEK VIASAK

References

[1]	"The blue copper protein gene of Alcaligenes faecalis S-6 directs secretion of blue copper protein from Escherichia coli cells." Yamamoto K., Uozumi T., Beppu T. J. Bacteriol. 169:5648-5652(1987) [PubMed: 2824441] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S-6.
[2]	"The amino acid sequence of the blue copper protein of Alcaligenes faecalis." Hormel S., Adman E.T., Walsh K.A., Beppu T., Titani K. FEBS Lett. 197:301-304(1986) [PubMed: 3512305] [Abstract] Cited for: PROTEIN SEQUENCE OF 24-146. Strain: S-6.
[3]	"The crystal structure of pseudoazurin from Alcaligenes faecalis S-6 determined at 2.9-A resolution." Petratos K., Banner D.W., Beppu T., Wilson K.S., Tsernoglou D. FEBS Lett. 218:209-214(1987) [PubMed: 3595868] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS). Strain: S-6.
[4]	"A 2.0-A structure of the blue copper protein (cupredoxin) from Alcaligenes faecalis S-6." Adman E.T., Turley S., Bramson R., Petratos K., Banner D., Tsernoglou D., Beppu T., Watanabe H. J. Biol. Chem. 264:87-99(1989) [PubMed: 2909547] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS). Strain: S-6.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M18267 Genomic DNA. Translation: AAA21955.1.

PIR

CUALBF. A28385.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1PAZ	X-ray	1.55	A	24-146	[»]
1PY0	X-ray	2.00	A	24-146	[»]
1PZA	X-ray	1.80	A	24-146	[»]
1PZB	X-ray	1.80	A	24-146	[»]
1PZC	X-ray	1.85	A	24-146	[»]
2P80	NMR	-	D	24-146	[»]
3NYK	X-ray	1.86	A	24-146	[»]
3PAZ	X-ray	1.73	A	24-146	[»]
4PAZ	X-ray	1.76	A	24-146	[»]
5PAZ	X-ray	1.76	A	24-146	[»]
6PAZ	X-ray	1.91	A	24-146	[»]
7PAZ	X-ray	2.00	A	24-146	[»]
8PAZ	X-ray	1.60	A	24-146	[»]

ProteinModelPortal

P04377.

SMR

P04377. Positions 24-146.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR002386. Amicyanin.
IPR000923. BlueCu_1.
IPR001235. Copper_blue.
IPR008972. Cupredoxin.
IPR012745. Pseudoazurin.
[Graphical view]

Gene3D

G3DSA:2.60.40.420. Cupredoxin. 1 hit.

Pfam

PF00127. Copper-bind. 1 hit.
[Graphical view]

PRINTS

PR00155. AMICYANIN.
PR00156. COPPERBLUE.

SUPFAM

SSF49503. Cupredoxin. 1 hit.

TIGRFAMs

TIGR02375. Pseudoazurin. 1 hit.

PROSITE

PS00196. COPPER_BLUE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

AZUP_ALCFA

Accession

Primary (citable) accession number: P04377

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 1987
Last sequence update:	July 1, 1989
Last modified:	May 31, 2011
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program