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P04364 (KITH_VAR67) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidine kinase

EC=2.7.1.21
Gene names
Name:TK
ORF Names:J2R, L2R, M2R
OrganismVariola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus) [Reference proteome]
Taxonomic identifier587200 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length177 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Phosphorylates thymidine and thymidine analogs, such as azidothymidine (AZT). Part of the salvage pathway for pyrimidine deoxyribonucleotide synthesis By similarity.

Catalytic activity

ATP + thymidine = ADP + thymidine 5'-phosphate.

Subunit structure

Homotetramer By similarity. Two molecules of substrate bind to each enzyme tetramer By similarity.

Sequence similarities

Belongs to the thymidine kinase family.

Ontologies

Keywords
   Biological processDNA synthesis
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

thymidine kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 177177Thymidine kinase
PRO_0000174941

Regions

Nucleotide binding11 – 188ATP By similarity
Region157 – 1615Substrate binding By similarity

Sites

Active site831Proton acceptor Potential
Metal binding1381Zinc By similarity
Metal binding1411Zinc By similarity
Metal binding1701Zinc By similarity
Metal binding1731Zinc By similarity
Binding site1131Substrate; via amide nitrogen By similarity

Natural variations

Natural variant901I → V in strain: Bangladesh-1975.
Natural variant1531T → A in strain: Garcia-1966.
Natural variant1631I → N in strain: Bangladesh-1975.

Sequences

Sequence LengthMass (Da)Tools
P04364 [UniParc].

Last modified October 1, 1993. Version 2.
Checksum: 580FF33308BE5A35

FASTA17720,055
        10         20         30         40         50         60 
MNGGHIQLII GPMFSGKSTE LIRRVRRYQI AQYKCVTIKY SNDNRYGTGL WTHDKNNFEA 

        70         80         90        100        110        120 
LEATKLCDVL EAITDFSVIG IDEGQFFPDI VEFCERMANE GKIVIVAALD GTFQRKPFNN 

       130        140        150        160        170 
ILDLIPLSEM VVKLTAVCMK CFKEASFSKR LGTETKIEII GGIDMYQSVC RKCYIDS 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence analysis of variola virus HindIII M, L, I genome fragments."
Shchelkunov S.N., Blinov V.M., Totmenin A.V., Marennikova S.S., Kolykhalov A.A., Frolov I.V., Chizhikov V.E., Gytorov V.V., Gashikov P.V., Belanov E.F., Belavin P.A., Resenchuk S.M., Andzhaparidze O.G., Sandakhchiev L.S.
Virus Res. 27:25-35(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Genes of variola and vaccinia viruses necessary to overcome the host protective mechanisms."
Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.
FEBS Lett. 319:80-83(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Nucleotide sequence of the thymidine kinase gene region of monkeypox and variola viruses."
Esposito J.J., Knight J.C.
Virology 135:561-567(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Bangladesh-1975.
[4]"Potential virulence determinants in terminal regions of variola smallpox virus genome."
Massung R.F., Esposito J.J., Liu L.I., Qi J., Utterback T.R., Knight J.C., Aubin L., Yuran T.E., Parsons J.M., Loparev V.N., Selivanov N.A., Cavallaro K.F., Kerlavage A.R., Mahy B.W.J., Venter J.C.
Nature 366:748-751(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Bangladesh-1975.
[5]"Alastrim smallpox variola minor virus genome DNA sequences."
Shchelkunov S.N., Totmenin A.V., Loparev V.N., Safronov P.F., Gutorov V.V., Chizhikov V.E., Knight J.C., Parsons J.M., Massung R.F., Esposito J.J.
Virology 266:361-386(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Garcia-1966.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X67119 Genomic DNA. Translation: CAA47578.1.
K02031 Genomic DNA. Translation: AAA80461.1.
S55844 Genomic DNA. Translation: AAB24675.1.
X69198 Genomic DNA. Translation: CAA49020.1.
L22579 Genomic DNA. Translation: AAA60827.1.
Y16780 Genomic DNA. Translation: CAB54679.1.
PIRKIVZVV. A00611.
E72160.
T28517.
RefSeqNP_042123.1. NC_001611.1.

3D structure databases

ProteinModelPortalP04364.
SMRP04364. Positions 4-175.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1486465.

Phylogenomic databases

ProtClustDBCLSP2509781.

Family and domain databases

InterProIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERPTHR11441. PTHR11441. 1 hit.
PfamPF00265. TK. 1 hit.
[Graphical view]
PIRSFPIRSF035805. TK_cell. 1 hit.
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKITH_VAR67
AccessionPrimary (citable) accession number: P04364
Secondary accession number(s): Q9QNJ1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: October 1, 1993
Last modified: April 16, 2014
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families