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P04364

- KITH_VAR67

UniProt

P04364 - KITH_VAR67

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Protein

Thymidine kinase

Gene

TK

Organism
Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Phosphorylates thymidine and thymidine analogs, such as azidothymidine (AZT). Part of the salvage pathway for pyrimidine deoxyribonucleotide synthesis (By similarity).By similarity

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei83 – 831Proton acceptorSequence Analysis
Binding sitei113 – 1131Substrate; via amide nitrogenBy similarity
Metal bindingi138 – 1381ZincBy similarity
Metal bindingi141 – 1411ZincBy similarity
Metal bindingi170 – 1701ZincBy similarity
Metal bindingi173 – 1731ZincBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 188ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. thymidine kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase (EC:2.7.1.21)
Gene namesi
Name:TK
ORF Names:J2R, L2R, M2R
OrganismiVariola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus)
Taxonomic identifieri587200 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000002060: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 177177Thymidine kinasePRO_0000174941Add
BLAST

Interactioni

Subunit structurei

Homotetramer. Two molecules of substrate bind to each enzyme tetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP04364.
SMRiP04364. Positions 4-175.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni157 – 1615Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the thymidine kinase family.Curated

Family and domain databases

InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04364-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNGGHIQLII GPMFSGKSTE LIRRVRRYQI AQYKCVTIKY SNDNRYGTGL
60 70 80 90 100
WTHDKNNFEA LEATKLCDVL EAITDFSVIG IDEGQFFPDI VEFCERMANE
110 120 130 140 150
GKIVIVAALD GTFQRKPFNN ILDLIPLSEM VVKLTAVCMK CFKEASFSKR
160 170
LGTETKIEII GGIDMYQSVC RKCYIDS
Length:177
Mass (Da):20,055
Last modified:October 1, 1993 - v2
Checksum:i580FF33308BE5A35
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti90 – 901I → V in strain: Bangladesh-1975.
Natural varianti153 – 1531T → A in strain: Garcia-1966.
Natural varianti163 – 1631I → N in strain: Bangladesh-1975.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67119 Genomic DNA. Translation: CAA47578.1.
K02031 Genomic DNA. Translation: AAA80461.1.
S55844 Genomic DNA. Translation: AAB24675.1.
X69198 Genomic DNA. Translation: CAA49020.1.
L22579 Genomic DNA. Translation: AAA60827.1.
Y16780 Genomic DNA. Translation: CAB54679.1.
PIRiA00611. KIVZVV.
E72160.
T28517.
RefSeqiNP_042123.1. NC_001611.1.

Genome annotation databases

GeneIDi1486465.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67119 Genomic DNA. Translation: CAA47578.1 .
K02031 Genomic DNA. Translation: AAA80461.1 .
S55844 Genomic DNA. Translation: AAB24675.1 .
X69198 Genomic DNA. Translation: CAA49020.1 .
L22579 Genomic DNA. Translation: AAA60827.1 .
Y16780 Genomic DNA. Translation: CAB54679.1 .
PIRi A00611. KIVZVV.
E72160.
T28517.
RefSeqi NP_042123.1. NC_001611.1.

3D structure databases

ProteinModelPortali P04364.
SMRi P04364. Positions 4-175.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1486465.

Family and domain databases

InterProi IPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view ]
PANTHERi PTHR11441. PTHR11441. 1 hit.
Pfami PF00265. TK. 1 hit.
[Graphical view ]
PIRSFi PIRSF035805. TK_cell. 1 hit.
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Genes of variola and vaccinia viruses necessary to overcome the host protective mechanisms."
    Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.
    FEBS Lett. 319:80-83(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Nucleotide sequence of the thymidine kinase gene region of monkeypox and variola viruses."
    Esposito J.J., Knight J.C.
    Virology 135:561-567(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Bangladesh-1975.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Bangladesh-1975.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Garcia-1966.

Entry informationi

Entry nameiKITH_VAR67
AccessioniPrimary (citable) accession number: P04364
Secondary accession number(s): Q9QNJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: October 1, 1993
Last modified: October 1, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3