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P04364

- KITH_VAR67

UniProt

P04364 - KITH_VAR67

Protein

Thymidine kinase

Gene

TK

Organism
Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 2 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Phosphorylates thymidine and thymidine analogs, such as azidothymidine (AZT). Part of the salvage pathway for pyrimidine deoxyribonucleotide synthesis By similarity.By similarity

    Catalytic activityi

    ATP + thymidine = ADP + thymidine 5'-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei83 – 831Proton acceptorSequence Analysis
    Binding sitei113 – 1131Substrate; via amide nitrogenBy similarity
    Metal bindingi138 – 1381ZincBy similarity
    Metal bindingi141 – 1411ZincBy similarity
    Metal bindingi170 – 1701ZincBy similarity
    Metal bindingi173 – 1731ZincBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi11 – 188ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. thymidine kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    DNA synthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidine kinase (EC:2.7.1.21)
    Gene namesi
    Name:TK
    ORF Names:J2R, L2R, M2R
    OrganismiVariola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus)
    Taxonomic identifieri587200 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000002060: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 177177Thymidine kinasePRO_0000174941Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer. Two molecules of substrate bind to each enzyme tetramer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP04364.
    SMRiP04364. Positions 4-175.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni157 – 1615Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the thymidine kinase family.Curated

    Family and domain databases

    InterProiIPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    [Graphical view]
    PANTHERiPTHR11441. PTHR11441. 1 hit.
    PfamiPF00265. TK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF035805. TK_cell. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P04364-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNGGHIQLII GPMFSGKSTE LIRRVRRYQI AQYKCVTIKY SNDNRYGTGL    50
    WTHDKNNFEA LEATKLCDVL EAITDFSVIG IDEGQFFPDI VEFCERMANE 100
    GKIVIVAALD GTFQRKPFNN ILDLIPLSEM VVKLTAVCMK CFKEASFSKR 150
    LGTETKIEII GGIDMYQSVC RKCYIDS 177
    Length:177
    Mass (Da):20,055
    Last modified:October 1, 1993 - v2
    Checksum:i580FF33308BE5A35
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti90 – 901I → V in strain: Bangladesh-1975.
    Natural varianti153 – 1531T → A in strain: Garcia-1966.
    Natural varianti163 – 1631I → N in strain: Bangladesh-1975.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X67119 Genomic DNA. Translation: CAA47578.1.
    K02031 Genomic DNA. Translation: AAA80461.1.
    S55844 Genomic DNA. Translation: AAB24675.1.
    X69198 Genomic DNA. Translation: CAA49020.1.
    L22579 Genomic DNA. Translation: AAA60827.1.
    Y16780 Genomic DNA. Translation: CAB54679.1.
    PIRiA00611. KIVZVV.
    E72160.
    T28517.
    RefSeqiNP_042123.1. NC_001611.1.

    Genome annotation databases

    GeneIDi1486465.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X67119 Genomic DNA. Translation: CAA47578.1 .
    K02031 Genomic DNA. Translation: AAA80461.1 .
    S55844 Genomic DNA. Translation: AAB24675.1 .
    X69198 Genomic DNA. Translation: CAA49020.1 .
    L22579 Genomic DNA. Translation: AAA60827.1 .
    Y16780 Genomic DNA. Translation: CAB54679.1 .
    PIRi A00611. KIVZVV.
    E72160.
    T28517.
    RefSeqi NP_042123.1. NC_001611.1.

    3D structure databases

    ProteinModelPortali P04364.
    SMRi P04364. Positions 4-175.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1486465.

    Family and domain databases

    InterProi IPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    [Graphical view ]
    PANTHERi PTHR11441. PTHR11441. 1 hit.
    Pfami PF00265. TK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF035805. TK_cell. 1 hit.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Genes of variola and vaccinia viruses necessary to overcome the host protective mechanisms."
      Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.
      FEBS Lett. 319:80-83(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "Nucleotide sequence of the thymidine kinase gene region of monkeypox and variola viruses."
      Esposito J.J., Knight J.C.
      Virology 135:561-567(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Bangladesh-1975.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Bangladesh-1975.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Garcia-1966.

    Entry informationi

    Entry nameiKITH_VAR67
    AccessioniPrimary (citable) accession number: P04364
    Secondary accession number(s): Q9QNJ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 20, 1987
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 83 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3