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P04363

- KITH_MONPV

UniProt

P04363 - KITH_MONPV

Protein

Thymidine kinase

Gene

TK

Organism
Monkeypox virus
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
  1. Functioni

    Catalytic activityi

    ATP + thymidine = ADP + thymidine 5'-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei83 – 831Proton acceptorSequence Analysis
    Binding sitei113 – 1131Substrate; via amide nitrogenBy similarity
    Metal bindingi138 – 1381ZincBy similarity
    Metal bindingi141 – 1411ZincBy similarity
    Metal bindingi170 – 1701ZincBy similarity
    Metal bindingi173 – 1731ZincBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi11 – 188ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. thymidine kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    DNA synthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidine kinase (EC:2.7.1.21)
    Gene namesi
    Name:TK
    ORF Names:L2R
    OrganismiMonkeypox virus
    Taxonomic identifieri10244 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirus
    Virus hostiCynomys gunnisoni (Gunnison's prairie dog) [TaxID: 45479]
    Cynomys leucurus (White-tailed prairie dog) [TaxID: 99825]
    Cynomys ludovicianus (Black-tailed prairie dog) [TaxID: 45480]
    Cynomys mexicanus (Mexican prairie dog) [TaxID: 99826]
    Cynomys parvidens (Utah prairie dog) [TaxID: 99827]
    Gliridae (dormice) [TaxID: 30650]
    Heliosciurus ruwenzorii (Ruwenzori sun squirrel) [TaxID: 226685]
    Homo sapiens (Human) [TaxID: 9606]
    Mus musculus (Mouse) [TaxID: 10090]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 177177Thymidine kinasePRO_0000174931Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP04363.
    SMRiP04363. Positions 4-175.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni157 – 1615Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the thymidine kinase family.Curated

    Family and domain databases

    InterProiIPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    [Graphical view]
    PANTHERiPTHR11441. PTHR11441. 1 hit.
    PfamiPF00265. TK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF035805. TK_cell. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P04363-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNGGHIQLII GPMLSGKSTE LIRRVRRYQI AQYKCVTIKY SNDNRYGTGL    50
    WTHDKNNFAA LEVTKLCDVL EAITDFSVIG IDEGQFFPDI VEFCERMANE 100
    GKIVIVAALD GTFQRRPFNN ILNLIPLSEM VVKLTAVCMK CFKEASFSKR 150
    LGAETEIEII GGNDMYQSVC RKCYIDS 177
    Length:177
    Mass (Da):19,990
    Last modified:August 2, 2002 - v2
    Checksum:i8A7BAAB3238C91D2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti14 – 141L → F in AAA80529. (PubMed:6330986)Curated
    Sequence conflicti90 – 901I → V in AAA80529. (PubMed:6330986)Curated
    Sequence conflicti153 – 1531A → T in AAA80529. (PubMed:6330986)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K02025 Genomic DNA. Translation: AAA80529.1.
    AF380138 Genomic DNA. Translation: AAL40544.1.
    PIRiA00610. KIVZMV.
    RefSeqiNP_536513.1. NC_003310.1.

    Genome annotation databases

    GeneIDi928935.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K02025 Genomic DNA. Translation: AAA80529.1 .
    AF380138 Genomic DNA. Translation: AAL40544.1 .
    PIRi A00610. KIVZMV.
    RefSeqi NP_536513.1. NC_003310.1.

    3D structure databases

    ProteinModelPortali P04363.
    SMRi P04363. Positions 4-175.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 928935.

    Family and domain databases

    InterProi IPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    [Graphical view ]
    PANTHERi PTHR11441. PTHR11441. 1 hit.
    Pfami PF00265. TK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF035805. TK_cell. 1 hit.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the thymidine kinase gene region of monkeypox and variola viruses."
      Esposito J.J., Knight J.C.
      Virology 135:561-567(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Isolate Zaire-96-I-16.

    Entry informationi

    Entry nameiKITH_MONPV
    AccessioniPrimary (citable) accession number: P04363
    Secondary accession number(s): Q8V4Z6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 20, 1987
    Last sequence update: August 2, 2002
    Last modified: October 1, 2014
    This is version 68 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3