P04363 (KITH_MONPV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
EC=2.7.1.21

Gene names

Name:	TK
ORF Names:	L2R

Organism

Monkeypox virus

Taxonomic identifier

10244 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus

Virus host

Cynomys gunnisoni (Gunnison's prairie dog) [TaxID: 45479]
Cynomys leucurus (White-tailed prairie dog) [TaxID: 99825]
Cynomys ludovicianus (Black-tailed prairie dog) [TaxID: 45480]
Cynomys mexicanus (Mexican prairie dog) [TaxID: 99826]
Cynomys parvidens (Utah prairie dog) [TaxID: 99827]
Gliridae (dormice) [TaxID: 30650]
Heliosciurus ruwenzorii (Ruwenzori sun squirrel) [TaxID: 226685]
Homo sapiens (Human) [TaxID: 9606]
Mus musculus (Mouse) [TaxID: 10090]

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate.
Sequence similarities	Belongs to the thymidine kinase family.

Ontologies

Keywords
Biological process	DNA synthesis
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW thymidine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 177	177	Thymidine kinase		PRO_0000174931
Regions
	Nucleotide binding	11 – 18	8	ATP By similarity
	Region	157 – 161	5	Substrate binding By similarity
Sites
	Active site	83	1	Proton acceptor Potential
	Metal binding	138	1	Zinc By similarity
	Metal binding	141	1	Zinc By similarity
	Metal binding	170	1	Zinc By similarity
	Metal binding	173	1	Zinc By similarity
	Binding site	113	1	Substrate; via amide nitrogen By similarity
Experimental info
	Sequence conflict	14	1	L → F in AAA80529. Ref.1
	Sequence conflict	90	1	I → V in AAA80529. Ref.1
	Sequence conflict	153	1	A → T in AAA80529. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P04363 [UniParc]. Last modified August 2, 2002. Version 2. Checksum: 8A7BAAB3238C91D2	FASTA	177	19,990
	10 20 30 40 50 60 MNGGHIQLII GPMLSGKSTE LIRRVRRYQI AQYKCVTIKY SNDNRYGTGL WTHDKNNFAA 70 80 90 100 110 120 LEVTKLCDVL EAITDFSVIG IDEGQFFPDI VEFCERMANE GKIVIVAALD GTFQRRPFNN 130 140 150 160 170 ILNLIPLSEM VVKLTAVCMK CFKEASFSKR LGAETEIEII GGNDMYQSVC RKCYIDS

References

[1]	"Nucleotide sequence of the thymidine kinase gene region of monkeypox and variola viruses." Esposito J.J., Knight J.C. Virology 135:561-567(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Human monkeypox and smallpox viruses: genomic comparison." Shchelkunov S.N., Totmenin A.V., Babkin I.V., Safronov P.F., Ryazankina O.I., Petrov N.A., Gutorov V.V., Uvarova E.A., Mikheev M.V., Sisler J.R., Esposito J.J., Jahrling P.B., Moss B., Sandakhchiev L.S. FEBS Lett. 509:66-70(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Isolate Zaire-96-I-16.

Cross-references

Sequence databases
EMBL GenBank DDBJ	K02025 Genomic DNA. Translation: AAA80529.1. AF380138 Genomic DNA. Translation: AAL40544.1.
PIR	KIVZMV. A00610.
RefSeq	NP_536513.1. NC_003310.1.
3D structure databases
ProteinModelPortal	P04363.
SMR	P04363. Positions 4-175.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	928935.
Phylogenomic databases
ProtClustDB	CLSP2509781.
Family and domain databases
InterPro	IPR001267. Thymidine_kinase. IPR020633. Thymidine_kinase_CS. [Graphical view]
PANTHER	PTHR11441. PTHR11441. 1 hit.
Pfam	PF00265. TK. 1 hit. [Graphical view]
PIRSF	PIRSF035805. TK_cell. 1 hit.
PROSITE	PS00603. TK_CELLULAR_TYPE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KITH_MONPV

Accession

Primary (citable) accession number: P04363
Secondary accession number(s): Q8V4Z6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 1987
Last sequence update:	August 2, 2002
Last modified:	April 3, 2013
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program