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Protein

Metallothionein-2

Gene

Mt2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi5 – 51Divalent metal cation; cluster B
Metal bindingi7 – 71Divalent metal cation; cluster B
Metal bindingi13 – 131Divalent metal cation; cluster B
Metal bindingi15 – 151Divalent metal cation; cluster B
Metal bindingi19 – 191Divalent metal cation; cluster B
Metal bindingi21 – 211Divalent metal cation; cluster B
Metal bindingi24 – 241Divalent metal cation; cluster B
Metal bindingi26 – 261Divalent metal cation; cluster B
Metal bindingi29 – 291Divalent metal cation; cluster B
Metal bindingi33 – 331Divalent metal cation; cluster A
Metal bindingi34 – 341Divalent metal cation; cluster A
Metal bindingi36 – 361Divalent metal cation; cluster A
Metal bindingi37 – 371Divalent metal cation; cluster A
Metal bindingi41 – 411Divalent metal cation; cluster A
Metal bindingi44 – 441Divalent metal cation; cluster A
Metal bindingi48 – 481Divalent metal cation; cluster A
Metal bindingi50 – 501Divalent metal cation; cluster A
Metal bindingi57 – 571Divalent metal cation; cluster A
Metal bindingi59 – 591Divalent metal cation; cluster A
Metal bindingi60 – 601Divalent metal cation; cluster A

GO - Molecular functioni

  • cadmium ion binding Source: RGD
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • astrocyte activation Source: RGD
  • cellular response to zinc ion Source: UniProtKB
  • negative regulation of growth Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Metal-thiolate cluster

Names & Taxonomyi

Protein namesi
Recommended name:
Metallothionein-2
Short name:
MT-2
Alternative name(s):
Metallothionein-II
Short name:
MT-II
Gene namesi
Name:Mt2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 19

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6161Metallothionein-2PRO_0000197222Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication
Modified residuei58 – 581PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP04355.
PRIDEiP04355.

Expressioni

Gene expression databases

ExpressionAtlasiP04355. baseline and differential.
GenevisibleiP04355. RN.

Interactioni

Protein-protein interaction databases

IntActiP04355. 1 interaction.
STRINGi10116.ENSRNOP00000062585.

Structurei

Secondary structure

1
61
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi9 – 113Combined sources
Beta strandi16 – 183Combined sources
Beta strandi35 – 373Combined sources
Turni38 – 403Combined sources
Turni42 – 465Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MRTNMR-A31-61[»]
2MRTNMR-A1-30[»]
4MT2X-ray2.00A1-61[»]
ProteinModelPortaliP04355.
SMRiP04355. Positions 1-61.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04355.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2929BetaAdd
BLAST
Regioni30 – 6132AlphaAdd
BLAST

Domaini

Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG138665.
GeneTreeiENSGT00730000110883.
HOGENOMiHOG000236262.
HOVERGENiHBG096105.
InParanoidiP04355.
KOiK14739.
OMAiCKKSCCT.
OrthoDBiEOG79CZ35.
PhylomeDBiP04355.
TreeFamiTF336054.

Family and domain databases

Gene3Di4.10.10.10. 1 hit.
InterProiIPR017854. Metalthion_dom.
IPR023587. Metalthion_dom_vert.
IPR003019. Metalthion_sfam_euk.
IPR000006. Metalthion_vert.
IPR018064. Metalthion_vert_metal_BS.
[Graphical view]
PANTHERiPTHR23299. PTHR23299. 1 hit.
PfamiPF00131. Metallothio. 1 hit.
[Graphical view]
PRINTSiPR00860. MTVERTEBRATE.
SUPFAMiSSF57868. SSF57868. 1 hit.
PROSITEiPS00203. METALLOTHIONEIN_VRT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04355-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPNCSCATD GSCSCAGSCK CKQCKCTSCK KSCCSCCPVG CAKCSQGCIC
60
KEASDKCSCC A
Length:61
Mass (Da):6,145
Last modified:March 20, 1987 - v1
Checksum:iD50D1306A5ED08E9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11794 Genomic DNA. Translation: AAA41640.1.
PIRiB61561. SMRT2.
RefSeqiNP_001131036.1. NM_001137564.1.
UniGeneiRn.115549.

Genome annotation databases

EnsembliENSRNOT00000067391; ENSRNOP00000062585; ENSRNOG00000043098.
GeneIDi689415.
KEGGirno:689415.
UCSCiRGD:1592345. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11794 Genomic DNA. Translation: AAA41640.1.
PIRiB61561. SMRT2.
RefSeqiNP_001131036.1. NM_001137564.1.
UniGeneiRn.115549.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MRTNMR-A31-61[»]
2MRTNMR-A1-30[»]
4MT2X-ray2.00A1-61[»]
ProteinModelPortaliP04355.
SMRiP04355. Positions 1-61.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP04355. 1 interaction.
STRINGi10116.ENSRNOP00000062585.

Proteomic databases

PaxDbiP04355.
PRIDEiP04355.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000067391; ENSRNOP00000062585; ENSRNOG00000043098.
GeneIDi689415.
KEGGirno:689415.
UCSCiRGD:1592345. rat.

Organism-specific databases

CTDi4502.

Phylogenomic databases

eggNOGiNOG138665.
GeneTreeiENSGT00730000110883.
HOGENOMiHOG000236262.
HOVERGENiHBG096105.
InParanoidiP04355.
KOiK14739.
OMAiCKKSCCT.
OrthoDBiEOG79CZ35.
PhylomeDBiP04355.
TreeFamiTF336054.

Miscellaneous databases

EvolutionaryTraceiP04355.
NextBioi738628.
PROiP04355.

Gene expression databases

ExpressionAtlasiP04355. baseline and differential.
GenevisibleiP04355. RN.

Family and domain databases

Gene3Di4.10.10.10. 1 hit.
InterProiIPR017854. Metalthion_dom.
IPR023587. Metalthion_dom_vert.
IPR003019. Metalthion_sfam_euk.
IPR000006. Metalthion_vert.
IPR018064. Metalthion_vert_metal_BS.
[Graphical view]
PANTHERiPTHR23299. PTHR23299. 1 hit.
PfamiPF00131. Metallothio. 1 hit.
[Graphical view]
PRINTSiPR00860. MTVERTEBRATE.
SUPFAMiSSF57868. SSF57868. 1 hit.
PROSITEiPS00203. METALLOTHIONEIN_VRT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Structural characterization of the isoforms of neonatal and adult rat liver metallothionein."
    Winge D.R., Nielson K.B., Zeikus R.D., Gray W.R.
    J. Biol. Chem. 259:11419-11425(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, ACETYLATION AT MET-1.
    Tissue: Liver.
  2. "Rat metallothionein multigene family."
    Andersen R.D., Taplitz S.J., Birren B.W., Bristol G., Herschman H.R.
    Experientia Suppl. 52:373-384(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Single crystals of cadmium, zinc metallothionein."
    Melis K.A., Carter D.C., Stout C.D., Winge D.R.
    J. Biol. Chem. 258:6255-6257(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: CRYSTALLIZATION.
  4. Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
  5. "Refined crystal structure of Cd, Zn metallothionein at 2.0-A resolution."
    Robbins A.H., McRee D.E., Williamson M., Collett S.A., Xuong N.H., Furey W.F. Jr., Wang B.C., Stout C.D.
    J. Mol. Biol. 221:1269-1293(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
  6. "Conformation of [Cd7]-metallothionein-2 from rat liver in aqueous solution determined by nuclear magnetic resonance spectroscopy."
    Schultze P., Woergotter E., Braun W., Wagner G., Vasak M., Kaegi J.H.R., Wuethrich K.
    J. Mol. Biol. 203:251-268(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.
  7. "Sequence-specific 1H-NMR assignments in rat-liver metallothionein-2."
    Woergoetter E., Wagner G., Vasak M., Kaegi J.H.R., Wuethrich K.
    Eur. J. Biochem. 167:457-466(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.

Entry informationi

Entry nameiMT2_RAT
AccessioniPrimary (citable) accession number: P04355
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: March 20, 1987
Last modified: June 24, 2015
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Metallothioneins
    Classification of metallothioneins and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.