P04355 (MT2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Metallothionein-2
Short name=MT-2

Alternative name(s):
Metallothionein-II
Short name=MT-II

Gene names

Name:

Mt2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	61 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
Domain	Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.
Sequence similarities	Belongs to the metallothionein superfamily. Type 1 family.

Ontologies

Keywords
Ligand	Metal-binding Metal-thiolate cluster
PTM	Acetylation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	astrocyte activation Inferred from expression pattern PubMed 19837066. Source: RGD cellular response to zinc ion Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of growth Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB perinuclear region of cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	cadmium ion binding Inferred from direct assay PubMed 1438200. Source: RGD zinc ion binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 61

61

Metallothionein-2

PRO_0000197222

Regions

Region

1 – 29

29

Beta

Region

30 – 61

32

Alpha

Sites

Metal binding

5

1

Divalent metal cation; cluster B

Metal binding

7

1

Divalent metal cation; cluster B

Metal binding

13

1

Divalent metal cation; cluster B

Metal binding

15

1

Divalent metal cation; cluster B

Metal binding

19

1

Divalent metal cation; cluster B

Metal binding

21

1

Divalent metal cation; cluster B

Metal binding

24

1

Divalent metal cation; cluster B

Metal binding

26

1

Divalent metal cation; cluster B

Metal binding

29

1

Divalent metal cation; cluster B

Metal binding

33

1

Divalent metal cation; cluster A

Metal binding

34

1

Divalent metal cation; cluster A

Metal binding

36

1

Divalent metal cation; cluster A

Metal binding

37

1

Divalent metal cation; cluster A

Metal binding

41

1

Divalent metal cation; cluster A

Metal binding

44

1

Divalent metal cation; cluster A

Metal binding

48

1

Divalent metal cation; cluster A

Metal binding

50

1

Divalent metal cation; cluster A

Metal binding

57

1

Divalent metal cation; cluster A

Metal binding

59

1

Divalent metal cation; cluster A

Metal binding

60

1

Divalent metal cation; cluster A

Amino acid modifications

Modified residue

1

1

N-acetylmethionine Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

61

Helix Strand Turn

Details...

Beta strand

9 – 11

3

Beta strand

16 – 18

3

Beta strand

35 – 37

3

Turn

38 – 40

3

Turn

42 – 46

5

Sequences

	Sequence		Length	Mass (Da)
	P04355 [UniParc]. Last modified March 20, 1987. Version 1. Checksum: D50D1306A5ED08E9	FASTA	61	6,145
	10 20 30 40 50 60 MDPNCSCATD GSCSCAGSCK CKQCKCTSCK KSCCSCCPVG CAKCSQGCIC KEASDKCSCC A

References

[1]	"Structural characterization of the isoforms of neonatal and adult rat liver metallothionein." Winge D.R., Nielson K.B., Zeikus R.D., Gray W.R. J. Biol. Chem. 259:11419-11425(1984) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Liver.
[2]	"Rat metallothionein multigene family." Andersen R.D., Taplitz S.J., Birren B.W., Bristol G., Herschman H.R. Experientia Suppl. 52:373-384(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Single crystals of cadmium, zinc metallothionein." Melis K.A., Carter D.C., Stout C.D., Winge D.R. J. Biol. Chem. 258:6255-6257(1983) [PubMed] [Europe PMC] [Abstract] Cited for: CRYSTALLIZATION.
[4]	"Crystal structure of Cd,Zn metallothionein." Furey W.F. Jr., Robbins A.H., Clancy L.L., Winge D.R., Wand B.C., Stout C.D. Science 231:704-710(1986) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
[5]	"Refined crystal structure of Cd, Zn metallothionein at 2.0-A resolution." Robbins A.H., McRee D.E., Williamson M., Collett S.A., Xuong N.H., Furey W.F. Jr., Wang B.C., Stout C.D. J. Mol. Biol. 221:1269-1293(1991) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
[6]	"Conformation of [Cd7]-metallothionein-2 from rat liver in aqueous solution determined by nuclear magnetic resonance spectroscopy." Schultze P., Woergotter E., Braun W., Wagner G., Vasak M., Kaegi J.H.R., Wuethrich K. J. Mol. Biol. 203:251-268(1988) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
[7]	"Sequence-specific 1H-NMR assignments in rat-liver metallothionein-2." Woergoetter E., Wagner G., Vasak M., Kaegi J.H.R., Wuethrich K. Eur. J. Biochem. 167:457-466(1987) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M11794 Genomic DNA. Translation: AAA41640.1.

IPI

IPI00195036.

PIR

SMRT2. B61561.

RefSeq

NP_001131036.1. NM_001137564.1.

UniGene

Rn.115549.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1MRT	NMR	-	A	31-61	[»]
2MRT	NMR	-	A	1-30	[»]
4MT2	X-ray	2.00	A	1-61	[»]

ProteinModelPortal

P04355.

SMR

P04355. Positions 1-61.

ModBase

Search...

Protein-protein interaction databases

IntAct

P04355. 1 interaction.

Proteomic databases

PaxDb

P04355.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSRNOT00000067391; ENSRNOP00000062585; ENSRNOG00000043098.

GeneID

689415.

KEGG

rno:689415.

UCSC

RGD:1592345. rat.

Organism-specific databases

CTD

4502.

Phylogenomic databases

eggNOG

NOG138665.

GeneTree

ENSGT00390000010461.

HOGENOM

HOG000236262.

HOVERGEN

HBG096105.

InParanoid

P04355.

KO

K14739.

OrthoDB

EOG46WZB6.

Gene expression databases

ArrayExpress

P04355.

Genevestigator

P04355.

GermOnline

ENSRNOG00000028841. Rattus norvegicus.

Family and domain databases

Gene3D

4.10.10.10. 1 hit.

InterPro

IPR017854. Metalthion_dom.
IPR023587. Metalthion_dom_vert.
IPR003019. Metalthion_sfam_euk.
IPR000006. Metalthion_vert.
IPR018064. Metalthion_vert_metal_BS.
[Graphical view]

PANTHER

PTHR23299. PTHR23299. 1 hit.

Pfam

PF00131. Metallothio. 1 hit.
[Graphical view]

PRINTS

PR00860. MTVERTEBRATE.

SUPFAM

SSF57868. Metallothionein_sfam. 1 hit.

PROSITE

PS00203. METALLOTHIONEIN_VRT. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P04355.

NextBio

738628.

Entry information

Entry name

MT2_RAT

Accession

Primary (citable) accession number: P04355

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 1987
Last sequence update:	March 20, 1987
Last modified:	April 3, 2013
This is version 117 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program