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Protein

Metallothionein-2

Gene

Mt2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi5Divalent metal cation; cluster B1
Metal bindingi7Divalent metal cation; cluster B1
Metal bindingi13Divalent metal cation; cluster B1
Metal bindingi15Divalent metal cation; cluster B1
Metal bindingi19Divalent metal cation; cluster B1
Metal bindingi21Divalent metal cation; cluster B1
Metal bindingi24Divalent metal cation; cluster B1
Metal bindingi26Divalent metal cation; cluster B1
Metal bindingi29Divalent metal cation; cluster B1
Metal bindingi33Divalent metal cation; cluster A1
Metal bindingi34Divalent metal cation; cluster A1
Metal bindingi36Divalent metal cation; cluster A1
Metal bindingi37Divalent metal cation; cluster A1
Metal bindingi41Divalent metal cation; cluster A1
Metal bindingi44Divalent metal cation; cluster A1
Metal bindingi48Divalent metal cation; cluster A1
Metal bindingi50Divalent metal cation; cluster A1
Metal bindingi57Divalent metal cation; cluster A1
Metal bindingi59Divalent metal cation; cluster A1
Metal bindingi60Divalent metal cation; cluster A1

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandMetal-binding, Metal-thiolate cluster

Enzyme and pathway databases

ReactomeiR-RNO-5661231 Metallothioneins bind metals

Names & Taxonomyi

Protein namesi
Recommended name:
Metallothionein-2
Short name:
MT-2
Alternative name(s):
Metallothionein-II
Short name:
MT-II
Gene namesi
Name:Mt2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 19

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001972221 – 61Metallothionein-2Add BLAST61

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1
Modified residuei58PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP04355
PRIDEiP04355

PTM databases

iPTMnetiP04355

Expressioni

Gene expression databases

BgeeiENSRNOG00000043098
ExpressionAtlasiP04355 baseline
GenevisibleiP04355 RN

Interactioni

Protein-protein interaction databases

IntActiP04355, 1 interactor
STRINGi10116.ENSRNOP00000062585

Structurei

Secondary structure

161
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi9 – 11Combined sources3
Beta strandi16 – 18Combined sources3
Beta strandi35 – 37Combined sources3
Turni38 – 40Combined sources3
Turni42 – 46Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MRTNMR-A31-61[»]
2MRTNMR-A1-30[»]
4MT2X-ray2.00A1-61[»]
ProteinModelPortaliP04355
SMRiP04355
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04355

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 29BetaAdd BLAST29
Regioni30 – 61AlphaAdd BLAST32

Domaini

Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410JFS1 Eukaryota
ENOG41113QR LUCA
GeneTreeiENSGT00730000110883
HOGENOMiHOG000236262
HOVERGENiHBG096105
InParanoidiP04355
KOiK14739
OMAiCNCAGSC
PhylomeDBiP04355
TreeFamiTF336054

Family and domain databases

Gene3Di4.10.10.10, 1 hit
InterProiView protein in InterPro
IPR003019 Metalthion
IPR017854 Metalthion_dom_sf
IPR023587 Metalthion_dom_sf_vert
IPR000006 Metalthion_vert
IPR018064 Metalthion_vert_metal_BS
PANTHERiPTHR23299 PTHR23299, 1 hit
PfamiView protein in Pfam
PF00131 Metallothio, 1 hit
PRINTSiPR00860 MTVERTEBRATE
SUPFAMiSSF57868 SSF57868, 1 hit
PROSITEiView protein in PROSITE
PS00203 METALLOTHIONEIN_VRT, 1 hit

Sequencei

Sequence statusi: Complete.

P04355-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPNCSCATD GSCSCAGSCK CKQCKCTSCK KSCCSCCPVG CAKCSQGCIC
60
KEASDKCSCC A
Length:61
Mass (Da):6,145
Last modified:March 20, 1987 - v1
Checksum:iD50D1306A5ED08E9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11794 Genomic DNA Translation: AAA41640.1
PIRiB61561 SMRT2
RefSeqiNP_001131036.1, NM_001137564.1
UniGeneiRn.115549

Genome annotation databases

EnsembliENSRNOT00000067391; ENSRNOP00000062585; ENSRNOG00000043098
GeneIDi689415
KEGGirno:689415
UCSCiRGD:1592345 rat

Similar proteinsi

Entry informationi

Entry nameiMT2_RAT
AccessioniPrimary (citable) accession number: P04355
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: March 20, 1987
Last modified: April 25, 2018
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health