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Protein

Metallothionein-2

Gene

Mt2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi5 – 51Divalent metal cation; cluster B
Metal bindingi7 – 71Divalent metal cation; cluster B
Metal bindingi13 – 131Divalent metal cation; cluster B
Metal bindingi15 – 151Divalent metal cation; cluster B
Metal bindingi19 – 191Divalent metal cation; cluster B
Metal bindingi21 – 211Divalent metal cation; cluster B
Metal bindingi24 – 241Divalent metal cation; cluster B
Metal bindingi26 – 261Divalent metal cation; cluster B
Metal bindingi29 – 291Divalent metal cation; cluster B
Metal bindingi33 – 331Divalent metal cation; cluster A
Metal bindingi34 – 341Divalent metal cation; cluster A
Metal bindingi36 – 361Divalent metal cation; cluster A
Metal bindingi37 – 371Divalent metal cation; cluster A
Metal bindingi41 – 411Divalent metal cation; cluster A
Metal bindingi44 – 441Divalent metal cation; cluster A
Metal bindingi48 – 481Divalent metal cation; cluster A
Metal bindingi50 – 501Divalent metal cation; cluster A
Metal bindingi57 – 571Divalent metal cation; cluster A
Metal bindingi59 – 591Divalent metal cation; cluster A
Metal bindingi60 – 601Divalent metal cation; cluster A

GO - Molecular functioni

  1. cadmium ion binding Source: RGD
  2. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. astrocyte activation Source: RGD
  2. cellular response to zinc ion Source: UniProtKB
  3. negative regulation of growth Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Metal-thiolate cluster

Names & Taxonomyi

Protein namesi
Recommended name:
Metallothionein-2
Short name:
MT-2
Alternative name(s):
Metallothionein-II
Short name:
MT-II
Gene namesi
Name:Mt2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 19

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleus Source: UniProtKB
  3. perinuclear region of cytoplasm Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6161Metallothionein-2PRO_0000197222Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP04355.
PRIDEiP04355.

Expressioni

Gene expression databases

ExpressionAtlasiP04355. baseline.
GenevestigatoriP04355.

Interactioni

Protein-protein interaction databases

IntActiP04355. 1 interaction.

Structurei

Secondary structure

1
61
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi9 – 113Combined sources
Beta strandi16 – 183Combined sources
Beta strandi35 – 373Combined sources
Turni38 – 403Combined sources
Turni42 – 465Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MRTNMR-A31-61[»]
2MRTNMR-A1-30[»]
4MT2X-ray2.00A1-61[»]
ProteinModelPortaliP04355.
SMRiP04355. Positions 1-61.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04355.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2929BetaAdd
BLAST
Regioni30 – 6132AlphaAdd
BLAST

Domaini

Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG138665.
GeneTreeiENSGT00730000110883.
HOGENOMiHOG000236262.
HOVERGENiHBG096105.
InParanoidiP04355.
KOiK14739.
OMAiCKKSCCT.
OrthoDBiEOG79CZ35.
PhylomeDBiP04355.
TreeFamiTF336054.

Family and domain databases

Gene3Di4.10.10.10. 1 hit.
InterProiIPR017854. Metalthion_dom.
IPR023587. Metalthion_dom_vert.
IPR003019. Metalthion_sfam_euk.
IPR000006. Metalthion_vert.
IPR018064. Metalthion_vert_metal_BS.
[Graphical view]
PANTHERiPTHR23299. PTHR23299. 1 hit.
PfamiPF00131. Metallothio. 1 hit.
[Graphical view]
PRINTSiPR00860. MTVERTEBRATE.
SUPFAMiSSF57868. SSF57868. 1 hit.
PROSITEiPS00203. METALLOTHIONEIN_VRT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04355-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDPNCSCATD GSCSCAGSCK CKQCKCTSCK KSCCSCCPVG CAKCSQGCIC
60
KEASDKCSCC A
Length:61
Mass (Da):6,145
Last modified:March 20, 1987 - v1
Checksum:iD50D1306A5ED08E9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11794 Genomic DNA. Translation: AAA41640.1.
PIRiB61561. SMRT2.
RefSeqiNP_001131036.1. NM_001137564.1.
UniGeneiRn.115549.

Genome annotation databases

EnsembliENSRNOT00000067391; ENSRNOP00000062585; ENSRNOG00000043098.
GeneIDi689415.
KEGGirno:689415.
UCSCiRGD:1592345. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11794 Genomic DNA. Translation: AAA41640.1.
PIRiB61561. SMRT2.
RefSeqiNP_001131036.1. NM_001137564.1.
UniGeneiRn.115549.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MRTNMR-A31-61[»]
2MRTNMR-A1-30[»]
4MT2X-ray2.00A1-61[»]
ProteinModelPortaliP04355.
SMRiP04355. Positions 1-61.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP04355. 1 interaction.

Proteomic databases

PaxDbiP04355.
PRIDEiP04355.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000067391; ENSRNOP00000062585; ENSRNOG00000043098.
GeneIDi689415.
KEGGirno:689415.
UCSCiRGD:1592345. rat.

Organism-specific databases

CTDi4502.

Phylogenomic databases

eggNOGiNOG138665.
GeneTreeiENSGT00730000110883.
HOGENOMiHOG000236262.
HOVERGENiHBG096105.
InParanoidiP04355.
KOiK14739.
OMAiCKKSCCT.
OrthoDBiEOG79CZ35.
PhylomeDBiP04355.
TreeFamiTF336054.

Miscellaneous databases

EvolutionaryTraceiP04355.
NextBioi738628.

Gene expression databases

ExpressionAtlasiP04355. baseline.
GenevestigatoriP04355.

Family and domain databases

Gene3Di4.10.10.10. 1 hit.
InterProiIPR017854. Metalthion_dom.
IPR023587. Metalthion_dom_vert.
IPR003019. Metalthion_sfam_euk.
IPR000006. Metalthion_vert.
IPR018064. Metalthion_vert_metal_BS.
[Graphical view]
PANTHERiPTHR23299. PTHR23299. 1 hit.
PfamiPF00131. Metallothio. 1 hit.
[Graphical view]
PRINTSiPR00860. MTVERTEBRATE.
SUPFAMiSSF57868. SSF57868. 1 hit.
PROSITEiPS00203. METALLOTHIONEIN_VRT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Structural characterization of the isoforms of neonatal and adult rat liver metallothionein."
    Winge D.R., Nielson K.B., Zeikus R.D., Gray W.R.
    J. Biol. Chem. 259:11419-11425(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, ACETYLATION AT MET-1.
    Tissue: Liver.
  2. "Rat metallothionein multigene family."
    Andersen R.D., Taplitz S.J., Birren B.W., Bristol G., Herschman H.R.
    Experientia Suppl. 52:373-384(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Single crystals of cadmium, zinc metallothionein."
    Melis K.A., Carter D.C., Stout C.D., Winge D.R.
    J. Biol. Chem. 258:6255-6257(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: CRYSTALLIZATION.
  4. Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
  5. "Refined crystal structure of Cd, Zn metallothionein at 2.0-A resolution."
    Robbins A.H., McRee D.E., Williamson M., Collett S.A., Xuong N.H., Furey W.F. Jr., Wang B.C., Stout C.D.
    J. Mol. Biol. 221:1269-1293(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
  6. "Conformation of [Cd7]-metallothionein-2 from rat liver in aqueous solution determined by nuclear magnetic resonance spectroscopy."
    Schultze P., Woergotter E., Braun W., Wagner G., Vasak M., Kaegi J.H.R., Wuethrich K.
    J. Mol. Biol. 203:251-268(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.
  7. "Sequence-specific 1H-NMR assignments in rat-liver metallothionein-2."
    Woergoetter E., Wagner G., Vasak M., Kaegi J.H.R., Wuethrich K.
    Eur. J. Biochem. 167:457-466(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.

Entry informationi

Entry nameiMT2_RAT
AccessioniPrimary (citable) accession number: P04355
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: March 20, 1987
Last modified: January 7, 2015
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Metallothioneins
    Classification of metallothioneins and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.