Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P04355

- MT2_RAT

UniProt

P04355 - MT2_RAT

Protein

Metallothionein-2

Gene

Mt2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi5 – 51Divalent metal cation; cluster B
    Metal bindingi7 – 71Divalent metal cation; cluster B
    Metal bindingi13 – 131Divalent metal cation; cluster B
    Metal bindingi15 – 151Divalent metal cation; cluster B
    Metal bindingi19 – 191Divalent metal cation; cluster B
    Metal bindingi21 – 211Divalent metal cation; cluster B
    Metal bindingi24 – 241Divalent metal cation; cluster B
    Metal bindingi26 – 261Divalent metal cation; cluster B
    Metal bindingi29 – 291Divalent metal cation; cluster B
    Metal bindingi33 – 331Divalent metal cation; cluster A
    Metal bindingi34 – 341Divalent metal cation; cluster A
    Metal bindingi36 – 361Divalent metal cation; cluster A
    Metal bindingi37 – 371Divalent metal cation; cluster A
    Metal bindingi41 – 411Divalent metal cation; cluster A
    Metal bindingi44 – 441Divalent metal cation; cluster A
    Metal bindingi48 – 481Divalent metal cation; cluster A
    Metal bindingi50 – 501Divalent metal cation; cluster A
    Metal bindingi57 – 571Divalent metal cation; cluster A
    Metal bindingi59 – 591Divalent metal cation; cluster A
    Metal bindingi60 – 601Divalent metal cation; cluster A

    GO - Molecular functioni

    1. cadmium ion binding Source: RGD
    2. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. astrocyte activation Source: RGD
    2. cellular response to zinc ion Source: UniProtKB
    3. negative regulation of growth Source: UniProtKB

    Keywords - Ligandi

    Metal-binding, Metal-thiolate cluster

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Metallothionein-2
    Short name:
    MT-2
    Alternative name(s):
    Metallothionein-II
    Short name:
    MT-II
    Gene namesi
    Name:Mt2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 19

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: UniProtKB
    3. perinuclear region of cytoplasm Source: UniProtKB

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 6161Metallothionein-2PRO_0000197222Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP04355.
    PRIDEiP04355.

    Expressioni

    Gene expression databases

    GenevestigatoriP04355.

    Interactioni

    Protein-protein interaction databases

    IntActiP04355. 1 interaction.

    Structurei

    Secondary structure

    1
    61
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi9 – 113
    Beta strandi16 – 183
    Beta strandi35 – 373
    Turni38 – 403
    Turni42 – 465

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1MRTNMR-A31-61[»]
    2MRTNMR-A1-30[»]
    4MT2X-ray2.00A1-61[»]
    ProteinModelPortaliP04355.
    SMRiP04355. Positions 1-61.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP04355.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 2929BetaAdd
    BLAST
    Regioni30 – 6132AlphaAdd
    BLAST

    Domaini

    Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG138665.
    GeneTreeiENSGT00730000110883.
    HOGENOMiHOG000236262.
    HOVERGENiHBG096105.
    InParanoidiP04355.
    KOiK14739.
    OMAiNCAGSCK.
    OrthoDBiEOG79CZ35.
    PhylomeDBiP04355.
    TreeFamiTF336054.

    Family and domain databases

    Gene3Di4.10.10.10. 1 hit.
    InterProiIPR017854. Metalthion_dom.
    IPR023587. Metalthion_dom_vert.
    IPR003019. Metalthion_sfam_euk.
    IPR000006. Metalthion_vert.
    IPR018064. Metalthion_vert_metal_BS.
    [Graphical view]
    PANTHERiPTHR23299. PTHR23299. 1 hit.
    PfamiPF00131. Metallothio. 1 hit.
    [Graphical view]
    PRINTSiPR00860. MTVERTEBRATE.
    SUPFAMiSSF57868. SSF57868. 1 hit.
    PROSITEiPS00203. METALLOTHIONEIN_VRT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P04355-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDPNCSCATD GSCSCAGSCK CKQCKCTSCK KSCCSCCPVG CAKCSQGCIC   50
    KEASDKCSCC A 61
    Length:61
    Mass (Da):6,145
    Last modified:March 20, 1987 - v1
    Checksum:iD50D1306A5ED08E9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11794 Genomic DNA. Translation: AAA41640.1.
    PIRiB61561. SMRT2.
    RefSeqiNP_001131036.1. NM_001137564.1.
    UniGeneiRn.115549.

    Genome annotation databases

    EnsembliENSRNOT00000067391; ENSRNOP00000062585; ENSRNOG00000043098.
    GeneIDi689415.
    KEGGirno:689415.
    UCSCiRGD:1592345. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11794 Genomic DNA. Translation: AAA41640.1 .
    PIRi B61561. SMRT2.
    RefSeqi NP_001131036.1. NM_001137564.1.
    UniGenei Rn.115549.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1MRT NMR - A 31-61 [» ]
    2MRT NMR - A 1-30 [» ]
    4MT2 X-ray 2.00 A 1-61 [» ]
    ProteinModelPortali P04355.
    SMRi P04355. Positions 1-61.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P04355. 1 interaction.

    Proteomic databases

    PaxDbi P04355.
    PRIDEi P04355.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000067391 ; ENSRNOP00000062585 ; ENSRNOG00000043098 .
    GeneIDi 689415.
    KEGGi rno:689415.
    UCSCi RGD:1592345. rat.

    Organism-specific databases

    CTDi 4502.

    Phylogenomic databases

    eggNOGi NOG138665.
    GeneTreei ENSGT00730000110883.
    HOGENOMi HOG000236262.
    HOVERGENi HBG096105.
    InParanoidi P04355.
    KOi K14739.
    OMAi NCAGSCK.
    OrthoDBi EOG79CZ35.
    PhylomeDBi P04355.
    TreeFami TF336054.

    Miscellaneous databases

    EvolutionaryTracei P04355.
    NextBioi 738628.

    Gene expression databases

    Genevestigatori P04355.

    Family and domain databases

    Gene3Di 4.10.10.10. 1 hit.
    InterProi IPR017854. Metalthion_dom.
    IPR023587. Metalthion_dom_vert.
    IPR003019. Metalthion_sfam_euk.
    IPR000006. Metalthion_vert.
    IPR018064. Metalthion_vert_metal_BS.
    [Graphical view ]
    PANTHERi PTHR23299. PTHR23299. 1 hit.
    Pfami PF00131. Metallothio. 1 hit.
    [Graphical view ]
    PRINTSi PR00860. MTVERTEBRATE.
    SUPFAMi SSF57868. SSF57868. 1 hit.
    PROSITEi PS00203. METALLOTHIONEIN_VRT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural characterization of the isoforms of neonatal and adult rat liver metallothionein."
      Winge D.R., Nielson K.B., Zeikus R.D., Gray W.R.
      J. Biol. Chem. 259:11419-11425(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE, ACETYLATION AT MET-1.
      Tissue: Liver.
    2. "Rat metallothionein multigene family."
      Andersen R.D., Taplitz S.J., Birren B.W., Bristol G., Herschman H.R.
      Experientia Suppl. 52:373-384(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Single crystals of cadmium, zinc metallothionein."
      Melis K.A., Carter D.C., Stout C.D., Winge D.R.
      J. Biol. Chem. 258:6255-6257(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: CRYSTALLIZATION.
    4. Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
    5. "Refined crystal structure of Cd, Zn metallothionein at 2.0-A resolution."
      Robbins A.H., McRee D.E., Williamson M., Collett S.A., Xuong N.H., Furey W.F. Jr., Wang B.C., Stout C.D.
      J. Mol. Biol. 221:1269-1293(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
    6. "Conformation of [Cd7]-metallothionein-2 from rat liver in aqueous solution determined by nuclear magnetic resonance spectroscopy."
      Schultze P., Woergotter E., Braun W., Wagner G., Vasak M., Kaegi J.H.R., Wuethrich K.
      J. Mol. Biol. 203:251-268(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.
    7. "Sequence-specific 1H-NMR assignments in rat-liver metallothionein-2."
      Woergoetter E., Wagner G., Vasak M., Kaegi J.H.R., Wuethrich K.
      Eur. J. Biochem. 167:457-466(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.

    Entry informationi

    Entry nameiMT2_RAT
    AccessioniPrimary (citable) accession number: P04355
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 20, 1987
    Last sequence update: March 20, 1987
    Last modified: October 1, 2014
    This is version 126 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Metallothioneins
      Classification of metallothioneins and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3