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Protein

Calbindin

Gene

CALB1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Buffers cytosolic calcium. May stimulate a membrane Ca2+-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi25 – 36121Add
BLAST
Calcium bindingi112 – 123122Add
BLAST
Calcium bindingi156 – 167123Add
BLAST
Calcium bindingi200 – 211124Add
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding, Vitamin D

Names & Taxonomyi

Protein namesi
Recommended name:
Calbindin
Alternative name(s):
Calbindin D28
D-28K
Vitamin D-dependent calcium-binding protein, avian-type
Gene namesi
Name:CALB1
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 2

Subcellular locationi

GO - Cellular componenti

  • axon Source: Ensembl
  • cytosol Source: GO_Central
  • dendrite Source: Ensembl
  • extracellular exosome Source: Ensembl
  • neuronal cell body Source: Ensembl
  • neuron projection Source: GO_Central
  • nucleus Source: GO_Central
  • postsynaptic membrane Source: UniProtKB
  • vesicle membrane Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 262261CalbindinPRO_0000073476Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP04354.

Expressioni

Tissue specificityi

Highly abundant in supporting cells. Also present in hair cells.1 Publication

Gene expression databases

ExpressionAtlasiP04354. baseline and differential.

Interactioni

Protein-protein interaction databases

BioGridi676764. 1 interaction.
IntActiP04354. 1 interaction.
STRINGi9031.ENSGALP00000026395.

Structurei

3D structure databases

ProteinModelPortaliP04354.
SMRiP04354. Positions 3-262.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 4736EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini54 – 8936EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini99 – 13436EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini143 – 17836EF-hand 4PROSITE-ProRule annotationAdd
BLAST
Domaini187 – 22236EF-hand 5PROSITE-ProRule annotationAdd
BLAST

Domaini

This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium.

Sequence similaritiesi

Belongs to the calbindin family.Curated
Contains 5 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0027. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00390000013118.
HOGENOMiHOG000231866.
HOVERGENiHBG000855.
InParanoidiP04354.
KOiK14757.
OMAiFFRCQQL.
OrthoDBiEOG71G9VB.
PhylomeDBiP04354.
TreeFamiTF325083.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR029634. Calbindin.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PANTHERiPTHR19972:SF3. PTHR19972:SF3. 1 hit.
PfamiPF13405. EF-hand_6. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 2 hits.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 5 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04354-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAETHLQGV EISAAQFFEI WHHYDSDGNG YMDGKELQNF IQELQQARKK
60 70 80 90 100
AGLDLTPEMK AFVDQYGKAT DGKIGIVELA QVLPTEENFL LFFRCQQLKS
110 120 130 140 150
SEDFMQTWRK YDSDHSGFID SEELKSFLKD LLQKANKQIE DSKLTEYTEI
160 170 180 190 200
MLRMFDANND GKLELTELAR LLPVQENFLI KFQGVKMCAK EFNKAFEMYD
210 220 230 240 250
QDGNGYIDEN ELDALLKDLC EKNKKELDIN NLATYKKSIM ALSDGGKLYR
260
AELALILCAE EN
Length:262
Mass (Da):30,167
Last modified:January 23, 2007 - v3
Checksum:i52081141364F08A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03343 mRNA. Translation: CAA27049.1.
M14230 mRNA. Translation: AAA48659.1.
M31143
, M31139, M31140, M31141, M31142 Genomic DNA. Translation: AAA83832.1.
X06629
, X06630, X06631, X06632, X06633 Genomic DNA. Translation: CAA29843.1.
PIRiA40926. KLCHI.
RefSeqiNP_990844.1. NM_205513.1.
UniGeneiGga.3286.

Genome annotation databases

EnsembliENSGALT00000026445; ENSGALP00000026395; ENSGALG00000015914.
GeneIDi396519.
KEGGigga:396519.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03343 mRNA. Translation: CAA27049.1.
M14230 mRNA. Translation: AAA48659.1.
M31143
, M31139, M31140, M31141, M31142 Genomic DNA. Translation: AAA83832.1.
X06629
, X06630, X06631, X06632, X06633 Genomic DNA. Translation: CAA29843.1.
PIRiA40926. KLCHI.
RefSeqiNP_990844.1. NM_205513.1.
UniGeneiGga.3286.

3D structure databases

ProteinModelPortaliP04354.
SMRiP04354. Positions 3-262.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi676764. 1 interaction.
IntActiP04354. 1 interaction.
STRINGi9031.ENSGALP00000026395.

Proteomic databases

PaxDbiP04354.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000026445; ENSGALP00000026395; ENSGALG00000015914.
GeneIDi396519.
KEGGigga:396519.

Organism-specific databases

CTDi793.

Phylogenomic databases

eggNOGiKOG0027. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00390000013118.
HOGENOMiHOG000231866.
HOVERGENiHBG000855.
InParanoidiP04354.
KOiK14757.
OMAiFFRCQQL.
OrthoDBiEOG71G9VB.
PhylomeDBiP04354.
TreeFamiTF325083.

Miscellaneous databases

PROiP04354.

Gene expression databases

ExpressionAtlasiP04354. baseline and differential.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR029634. Calbindin.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PANTHERiPTHR19972:SF3. PTHR19972:SF3. 1 hit.
PfamiPF13405. EF-hand_6. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 2 hits.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 5 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Putative amino acid sequence of chick calcium-binding protein deduced from a complementary DNA sequence."
    Wilson P.W., Harding M., Lawson D.E.M.
    Nucleic Acids Res. 13:8867-8881(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The 28-kDa vitamin D-dependent calcium-binding protein has a six-domain structure."
    Hunziker W.
    Proc. Natl. Acad. Sci. U.S.A. 83:7578-7582(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Molecular structure of the chicken vitamin D-induced calbindin-D28K gene reveals eleven exons, six Ca2+-binding domains, and numerous promoter regulatory elements."
    Minghetti P.P., Cancela L., Fujisawa Y., Theofan G., Norman A.W.
    Mol. Endocrinol. 2:355-367(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Structure of chick chromosomal genes for calbindin and calretinin."
    Wilson P.W., Rogers J.H., Harding M., Pohl V., Pattyn G., Lawson D.E.M.
    J. Mol. Biol. 200:615-625(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "Chicken intestinal 28-kilodalton calbindin-D: complete amino acid sequence and structural considerations."
    Fullmer C.S., Wasserman R.H.
    Proc. Natl. Acad. Sci. U.S.A. 84:4772-4776(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-262, ACETYLATION AT THR-2.
  6. "Molecular markers for cell types of the inner ear and candidate genes for hearing disorders."
    Heller S., Sheane C.A., Javed Z., Hudspeth A.J.
    Proc. Natl. Acad. Sci. U.S.A. 95:11400-11405(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
    Strain: White leghorn.

Entry informationi

Entry nameiCALB1_CHICK
AccessioniPrimary (citable) accession number: P04354
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.