P04351 (IL2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Interleukin-2
Short name=IL-2

Alternative name(s):
T-cell growth factor
Short name=TCGF

Gene names

Name:	Il2
Synonyms:	Il-2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	169 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
Subcellular location	Secreted.
Polymorphism	The poly-Gln region is highly polymorphic.
Sequence similarities	Belongs to the IL-2 family.

Ontologies

Keywords
Biological process	Adaptive immunity Immunity
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Cytokine Growth factor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	elevation of cytosolic calcium ion concentration Inferred from electronic annotation. Source: Compara immune response Inferred from electronic annotation. Source: InterPro negative regulation of B cell apoptotic process Inferred from sequence orthology PubMed 9184696. Source: MGI negative regulation of heart contraction Inferred from electronic annotation. Source: Compara negative regulation of inflammatory response Inferred from mutant phenotype PubMed 7589135PubMed 8402910. Source: MGI negative regulation of lymphocyte proliferation Inferred from mutant phenotype PubMed 16227984. Source: MGI negative regulation of protein phosphorylation Inferred from electronic annotation. Source: Compara positive regulation of B cell proliferation Inferred from sequence orthology PubMed 9184696. Source: MGI positive regulation of activated T cell proliferation Inferred from direct assay PubMed 10485657PubMed 7584142. Source: MGI positive regulation of dendritic spine development Inferred from electronic annotation. Source: Compara positive regulation of immunoglobulin secretion Inferred from mutant phenotype PubMed 1830926. Source: MGI positive regulation of interferon-gamma production Inferred from direct assay PubMed 17213291. Source: MGI positive regulation of interleukin-17 production Inferred from electronic annotation. Source: Compara positive regulation of isotype switching to IgG isotypes Inferred from mutant phenotype PubMed 1830926. Source: MGI positive regulation of regulatory T cell differentiation Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 10485657. Source: MGI positive regulation of tyrosine phosphorylation of Stat5 protein Inferred from direct assay PubMed 10485657. Source: MGI protein kinase C-activating G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: Compara regulation of T cell homeostatic proliferation Inferred from mutant phenotype PubMed 16227984. Source: MGI
Cellular_component	extracellular space Inferred from direct assay PubMed 10072077PubMed 11728336PubMed 12874832PubMed 16769892PubMed 1830926PubMed 19139201PubMed 7688139PubMed 8612131PubMed 9492004. Source: MGI
Molecular_function	carbohydrate binding Inferred from electronic annotation. Source: Compara cytokine activity Inferred from direct assay PubMed 10485657PubMed 1588041. Source: MGI glycosphingolipid binding Inferred from electronic annotation. Source: Compara interleukin-2 receptor binding Inferred from direct assay PubMed 1588041PubMed 7584142Ref.10. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Il2rg	P34902	1	EBI-80538,EBI-80548

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20
	Chain	21 – 169	149	Interleukin-2		PRO_0000015493
Regions
	Compositional bias	35 – 46	12	Poly-Gln
Amino acid modifications
	Glycosylation	23	1	O-linked (GalNAc...) By similarity
	Disulfide bond	92 ↔ 140		By similarity
Natural variations
	Natural variant	26	1	S → P in strain: RF/J and CAST/Ei.
	Natural variant	30	1	S → P in strain: RF/J.
	Natural variant	32 – 39	8	AEAQQQQQ → SSSTAEA in strain: CAST/Ei.
	Natural variant	32 – 38	7	AEAQQQQ → SSSTAEA in strain: RF/J.
Experimental info
	Sequence conflict	108	1	Q → E in AAA39281. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P04351 [UniParc]. Last modified March 20, 1987. Version 1. Checksum: B3B17644F6EF83CA	FASTA	169	19,400
	10 20 30 40 50 60 MYSMQLASCV TLTLVLLVNS APTSSSTSSS TAEAQQQQQQ QQQQQQHLEQ LLMDLQELLS 70 80 90 100 110 120 RMENYRNLKL PRMLTFKFYL PKQATELKDL QCLEDELGPL RHVLDLTQSK SFQLEDAENF 130 140 150 160 ISNIRVTVVK LKGSDNTFEC QFDDESATVV DFLRRWIAFC QSIISTSPQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Organization and structure of the mouse interleukin-2 gene." Fuse A., Fujita T., Yasumitsu H., Kashima N., Hasegawa K., Taniguchi T. Nucleic Acids Res. 12:9323-9331(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BALB/c.
[2]	"Unique structure of murine interleukin-2 as deduced from cloned cDNAs." Kashima N., Nishi-Takaoka C., Fujita T., Taki S., Yamada G., Hamuro J., Taniguchi T. Nature 313:402-404(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: B10BR.
[3]	"Use of a cDNA expression vector for isolation of mouse interleukin 2 cDNA clones: expression of T-cell growth-factor activity after transfection of monkey cells." Yokota T., Arai N., Lee F., Rennick D., Mosmann T., Arai K. Proc. Natl. Acad. Sci. U.S.A. 82:68-72(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6.
[4]	"Cloning and structure of a mouse interleukin-2 chromosomal gene." Degrave W., Simons G., Devos R., Plaetinck G., Remaut E., Tavernier J., Fiers W. Mol. Biol. Rep. 11:57-61(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Congenic mapping of the type 1 diabetes locus, Idd3, to a 780-kb region of mouse chromosome 3: identification of a candidate segment of ancestral DNA by haplotype mapping." Lyons P.A., Armitage N., Argentina F., Denny P., Hill N.J., Lord C.J., Wilusz M.B., Peterson L.B., Wicker L.S., Todd J.A. Genome Res. 10:446-453(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C57BL/6.
[6]	Ma R.Z., Teuscher C. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J. Tissue: Spleen.
[7]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[8]	"A new cDNA sequence for the murine interleukin-2 gene." Matesanz F., Alcina A., Pellicer A. Biochim. Biophys. Acta 1132:335-336(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-63. Strain: RF. Tissue: Spleen.
[9]	"Existence of at least five interleukin-2 molecules in different mouse strains." Matesanz F., Alcina A., Pellicer A. Immunogenetics 38:300-303(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-63. Strain: CAST/Ei and RF/J. Tissue: Spleen.
[10]	"Definition and spatial location of mouse interleukin-2 residues that interact with its heterotrimeric receptor." Zurawski S., Vega F. Jr., Doyel E.L., Huyghe B., Flaherty K., McKay D.B., Zurawski G. EMBO J. 12:5113-5119(1993) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X01663 Genomic DNA. Translation: CAA25823.1. X01664 Genomic DNA. Translation: CAA25824.1. X01665 Genomic DNA. Translation: CAA25825.1. X01772 mRNA. Translation: CAA25909.1. K02292 mRNA. Translation: AAA39289.1. M16762, M16760, M16761 Genomic DNA. Translation: AAA39281.1. AF195956 Genomic DNA. Translation: AAF32272.1. AF065914 mRNA. Translation: AAD25890.1. AL662823 Genomic DNA. Translation: CAM18418.1. X66058 mRNA. Translation: CAA46854.1. L07574 Genomic DNA. Translation: AAA39326.1. L07576 Genomic DNA. Translation: AAA39328.1.
IPI	IPI00115183.
PIR	ICMS2. A93550. I54512. I68871.
RefSeq	NP_032392.1. NM_008366.3.
UniGene	Mm.14190.
3D structure databases
ProteinModelPortal	P04351.
SMR	P04351. Positions 21-166.
ModBase	Search...
Protein-protein interaction databases
IntAct	P04351. 1 interaction.
PTM databases
PhosphoSite	P04351.
Proteomic databases
PRIDE	P04351.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000029275; ENSMUSP00000029275; ENSMUSG00000027720.
GeneID	16183.
KEGG	mmu:16183.
Organism-specific databases
CTD	3558.
MGI	MGI:96548. Il2.
Phylogenomic databases
eggNOG	NOG41211.
GeneTree	ENSGT00390000003555.
HOGENOM	HOG000048727.
HOVERGEN	HBG007496.
InParanoid	P04351.
KO	K05429.
OMA	KWITFCQ.
OrthoDB	EOG46Q6V2.
Gene expression databases
ArrayExpress	P04351.
Bgee	P04351.
CleanEx	MM_IL2.
Genevestigator	P04351.
GermOnline	ENSMUSG00000027720. Mus musculus.
Family and domain databases
Gene3D	1.20.1250.10. 1 hit.
InterPro	IPR009079. 4_helix_cytokine-like_core. IPR012351. 4_helix_cytokine_core. IPR000779. Interleukin-2. [Graphical view]
PANTHER	PTHR11443. PTHR11443. 1 hit.
Pfam	PF00715. IL2. 1 hit. [Graphical view]
PRINTS	PR00265. INTERLEUKIN2.
ProDom	PD003649. Interleukin-2. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00189. IL2. 1 hit. [Graphical view]
SUPFAM	SSF47266. 4_helix_cytokine. 1 hit.
PROSITE	PS00424. INTERLEUKIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P04351.
ChEMBL	CHEMBL2466.
NextBio	289069.
SOURCE	Search...

Entry information

Entry name

IL2_MOUSE

Accession

Primary (citable) accession number: P04351
Secondary accession number(s): P97945, Q791T3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 1987
Last sequence update:	March 20, 1987
Last modified:	May 1, 2013
This is version 119 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program