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P04351 (IL2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Interleukin-2

Short name=IL-2
Alternative name(s):
T-cell growth factor
Short name=TCGF
Gene names
Name:Il2
Synonyms:Il-2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length169 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.

Subcellular location

Secreted.

Polymorphism

The poly-Gln region is highly polymorphic.

Sequence similarities

Belongs to the IL-2 family.

Ontologies

Keywords
   Biological processAdaptive immunity
Immunity
   Cellular componentSecreted
   Coding sequence diversityPolymorphism
   DomainSignal
   Molecular functionCytokine
Growth factor
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processextrinsic apoptotic signaling pathway in absence of ligand

Inferred from direct assay PubMed 12082633. Source: MGI

immune response

Inferred from electronic annotation. Source: InterPro

negative regulation of B cell apoptotic process

Inferred from sequence orthology PubMed 9184696. Source: MGI

negative regulation of heart contraction

Inferred from electronic annotation. Source: Ensembl

negative regulation of inflammatory response

Inferred from mutant phenotype PubMed 7589135PubMed 8402910. Source: MGI

negative regulation of lymphocyte proliferation

Inferred from mutant phenotype PubMed 16227984. Source: MGI

negative regulation of protein phosphorylation

Inferred from electronic annotation. Source: Ensembl

positive regulation of B cell proliferation

Inferred from sequence orthology PubMed 9184696. Source: MGI

positive regulation of T cell proliferation

Inferred from direct assay PubMed 1588041. Source: MGI

positive regulation of activated T cell proliferation

Inferred from direct assay PubMed 10485657PubMed 7584142. Source: MGI

positive regulation of cytosolic calcium ion concentration

Inferred from electronic annotation. Source: Ensembl

positive regulation of dendritic spine development

Inferred from electronic annotation. Source: Ensembl

positive regulation of immunoglobulin secretion

Inferred from mutant phenotype PubMed 1830926. Source: MGI

positive regulation of interferon-gamma production

Inferred from direct assay PubMed 17213291. Source: MGI

positive regulation of interleukin-17 production

Inferred from electronic annotation. Source: Ensembl

positive regulation of isotype switching to IgG isotypes

Inferred from mutant phenotype PubMed 1830926. Source: MGI

positive regulation of regulatory T cell differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 10485657. Source: MGI

positive regulation of tyrosine phosphorylation of Stat5 protein

Inferred from direct assay PubMed 10485657. Source: MGI

protein kinase C-activating G-protein coupled receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

regulation of T cell homeostatic proliferation

Inferred from mutant phenotype PubMed 16227984. Source: MGI

   Cellular_componentextracellular space

Inferred from direct assay PubMed 10072077PubMed 11728336PubMed 12874832PubMed 16769892PubMed 1830926PubMed 19139201PubMed 7688139PubMed 8612131PubMed 9492004. Source: MGI

   Molecular_functioncarbohydrate binding

Inferred from electronic annotation. Source: Ensembl

cytokine activity

Inferred from direct assay PubMed 10485657PubMed 1588041. Source: MGI

glycosphingolipid binding

Inferred from electronic annotation. Source: Ensembl

interleukin-2 receptor binding

Inferred from direct assay PubMed 1588041PubMed 7584142Ref.10. Source: MGI

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Il2rgP349021EBI-80538,EBI-80548

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020
Chain21 – 169149Interleukin-2
PRO_0000015493

Regions

Compositional bias35 – 4612Poly-Gln

Amino acid modifications

Glycosylation231O-linked (GalNAc...) By similarity
Disulfide bond92 ↔ 140 By similarity

Natural variations

Natural variant261S → P in strain: RF/J and CAST/Ei.
Natural variant301S → P in strain: RF/J.
Natural variant32 – 398AEAQQQQQ → SSSTAEA in strain: CAST/Ei.
Natural variant32 – 387AEAQQQQ → SSSTAEA in strain: RF/J.

Experimental info

Sequence conflict1081Q → E in AAA39281. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P04351 [UniParc].

Last modified March 20, 1987. Version 1.
Checksum: B3B17644F6EF83CA

FASTA16919,400
        10         20         30         40         50         60 
MYSMQLASCV TLTLVLLVNS APTSSSTSSS TAEAQQQQQQ QQQQQQHLEQ LLMDLQELLS 

        70         80         90        100        110        120 
RMENYRNLKL PRMLTFKFYL PKQATELKDL QCLEDELGPL RHVLDLTQSK SFQLEDAENF 

       130        140        150        160 
ISNIRVTVVK LKGSDNTFEC QFDDESATVV DFLRRWIAFC QSIISTSPQ 

« Hide

References

« Hide 'large scale' references
[1]"Organization and structure of the mouse interleukin-2 gene."
Fuse A., Fujita T., Yasumitsu H., Kashima N., Hasegawa K., Taniguchi T.
Nucleic Acids Res. 12:9323-9331(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: BALB/c.
[2]"Unique structure of murine interleukin-2 as deduced from cloned cDNAs."
Kashima N., Nishi-Takaoka C., Fujita T., Taki S., Yamada G., Hamuro J., Taniguchi T.
Nature 313:402-404(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: B10BR.
[3]"Use of a cDNA expression vector for isolation of mouse interleukin 2 cDNA clones: expression of T-cell growth-factor activity after transfection of monkey cells."
Yokota T., Arai N., Lee F., Rennick D., Mosmann T., Arai K.
Proc. Natl. Acad. Sci. U.S.A. 82:68-72(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
[4]"Cloning and structure of a mouse interleukin-2 chromosomal gene."
Degrave W., Simons G., Devos R., Plaetinck G., Remaut E., Tavernier J., Fiers W.
Mol. Biol. Rep. 11:57-61(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"Congenic mapping of the type 1 diabetes locus, Idd3, to a 780-kb region of mouse chromosome 3: identification of a candidate segment of ancestral DNA by haplotype mapping."
Lyons P.A., Armitage N., Argentina F., Denny P., Hill N.J., Lord C.J., Wilusz M.B., Peterson L.B., Wicker L.S., Todd J.A.
Genome Res. 10:446-453(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: C57BL/6.
[6]Ma R.Z., Teuscher C.
Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6J.
Tissue: Spleen.
[7]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[8]"A new cDNA sequence for the murine interleukin-2 gene."
Matesanz F., Alcina A., Pellicer A.
Biochim. Biophys. Acta 1132:335-336(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-63.
Strain: RF.
Tissue: Spleen.
[9]"Existence of at least five interleukin-2 molecules in different mouse strains."
Matesanz F., Alcina A., Pellicer A.
Immunogenetics 38:300-303(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-63.
Strain: CAST/Ei and RF/J.
Tissue: Spleen.
[10]"Definition and spatial location of mouse interleukin-2 residues that interact with its heterotrimeric receptor."
Zurawski S., Vega F. Jr., Doyel E.L., Huyghe B., Flaherty K., McKay D.B., Zurawski G.
EMBO J. 12:5113-5119(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE MODELING.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X01663 Genomic DNA. Translation: CAA25823.1.
X01664 Genomic DNA. Translation: CAA25824.1.
X01665 Genomic DNA. Translation: CAA25825.1.
X01772 mRNA. Translation: CAA25909.1.
K02292 mRNA. Translation: AAA39289.1.
M16762, M16760, M16761 Genomic DNA. Translation: AAA39281.1.
AF195956 Genomic DNA. Translation: AAF32272.1.
AF065914 mRNA. Translation: AAD25890.1.
AL662823 Genomic DNA. Translation: CAM18418.1.
X66058 mRNA. Translation: CAA46854.1.
L07574 Genomic DNA. Translation: AAA39326.1.
L07576 Genomic DNA. Translation: AAA39328.1.
CCDSCCDS17316.1.
PIRICMS2. A93550.
I54512.
I68871.
RefSeqNP_032392.1. NM_008366.3.
UniGeneMm.14190.

3D structure databases

ProteinModelPortalP04351.
SMRP04351. Positions 21-166.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP04351. 1 interaction.

Chemistry

BindingDBP04351.
ChEMBLCHEMBL2466.

PTM databases

PhosphoSiteP04351.

Proteomic databases

PRIDEP04351.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000029275; ENSMUSP00000029275; ENSMUSG00000027720.
GeneID16183.
KEGGmmu:16183.
UCSCuc008pai.3. mouse.

Organism-specific databases

CTD3558.
MGIMGI:96548. Il2.

Phylogenomic databases

eggNOGNOG41211.
GeneTreeENSGT00390000003555.
HOGENOMHOG000048727.
HOVERGENHBG007496.
InParanoidP04351.
KOK05429.
OMAKWITFCQ.
OrthoDBEOG72RN13.
PhylomeDBP04351.
TreeFamTF338200.

Gene expression databases

BgeeP04351.
CleanExMM_IL2.
GenevestigatorP04351.

Family and domain databases

Gene3D1.20.1250.10. 1 hit.
InterProIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR000779. IL-2.
[Graphical view]
PANTHERPTHR11443. PTHR11443. 1 hit.
PfamPF00715. IL2. 1 hit.
[Graphical view]
PRINTSPR00265. INTERLEUKIN2.
ProDomPD003649. Interleukin-2. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00189. IL2. 1 hit.
[Graphical view]
SUPFAMSSF47266. SSF47266. 1 hit.
PROSITEPS00424. INTERLEUKIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio289069.
PROP04351.
SOURCESearch...

Entry information

Entry nameIL2_MOUSE
AccessionPrimary (citable) accession number: P04351
Secondary accession number(s): P97945, Q791T3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: March 20, 1987
Last modified: July 9, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot