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Protein

Interleukin-2

Gene

Il2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.

GO - Molecular functioni

  • carbohydrate binding Source: Ensembl
  • cytokine activity Source: MGI
  • glycosphingolipid binding Source: Ensembl
  • interleukin-2 receptor binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Growth factor

Keywords - Biological processi

Adaptive immunity, Immunity

Enzyme and pathway databases

ReactomeiR-MMU-114604. GPVI-mediated activation cascade.
R-MMU-392451. G beta:gamma signalling through PI3Kgamma.
R-MMU-451927. Interleukin-2 signaling.
R-MMU-5673001. RAF/MAP kinase cascade.
R-MMU-912526. Interleukin receptor SHC signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Interleukin-2
Short name:
IL-2
Alternative name(s):
T-cell growth factor
Short name:
TCGF
Gene namesi
Name:Il2
Synonyms:Il-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:96548. Il2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2466.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Add
BLAST
Chaini21 – 169149Interleukin-2PRO_0000015493Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi23 – 231O-linked (GalNAc...)By similarity
Disulfide bondi92 ↔ 140By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP04351.
PRIDEiP04351.

PTM databases

PhosphoSiteiP04351.

Expressioni

Gene expression databases

BgeeiP04351.
CleanExiMM_IL2.
GenevisibleiP04351. MM.

Interactioni

GO - Molecular functioni

  • cytokine activity Source: MGI
  • interleukin-2 receptor binding Source: MGI

Protein-protein interaction databases

IntActiP04351. 1 interaction.
STRINGi10090.ENSMUSP00000029275.

Chemistry

BindingDBiP04351.

Structurei

Secondary structure

1
169
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi47 – 6014Combined sources
Helixi70 – 734Combined sources
Beta strandi75 – 795Combined sources
Helixi87 – 904Combined sources
Helixi91 – 944Combined sources
Helixi97 – 1037Combined sources
Helixi118 – 13215Combined sources
Beta strandi134 – 1363Combined sources
Beta strandi144 – 1485Combined sources
Helixi149 – 16416Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4YQXX-ray2.83A/M47-169[»]
4YUEX-ray2.19C47-169[»]
ProteinModelPortaliP04351.
SMRiP04351. Positions 47-167.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi35 – 4612Poly-GlnAdd
BLAST

Sequence similaritiesi

Belongs to the IL-2 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J049. Eukaryota.
ENOG41119SF. LUCA.
GeneTreeiENSGT00390000003555.
HOGENOMiHOG000048727.
HOVERGENiHBG007496.
InParanoidiP04351.
KOiK05429.
OMAiLMSNINV.
OrthoDBiEOG72RN13.
PhylomeDBiP04351.
TreeFamiTF338200.

Family and domain databases

Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR000779. IL-2.
IPR030477. IL-2_CS.
[Graphical view]
PfamiPF00715. IL2. 1 hit.
[Graphical view]
PRINTSiPR00265. INTERLEUKIN2.
ProDomiPD003649. Interleukin-2. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00189. IL2. 1 hit.
[Graphical view]
SUPFAMiSSF47266. SSF47266. 1 hit.
PROSITEiPS00424. INTERLEUKIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04351-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYSMQLASCV TLTLVLLVNS APTSSSTSSS TAEAQQQQQQ QQQQQQHLEQ
60 70 80 90 100
LLMDLQELLS RMENYRNLKL PRMLTFKFYL PKQATELKDL QCLEDELGPL
110 120 130 140 150
RHVLDLTQSK SFQLEDAENF ISNIRVTVVK LKGSDNTFEC QFDDESATVV
160
DFLRRWIAFC QSIISTSPQ
Length:169
Mass (Da):19,400
Last modified:March 20, 1987 - v1
Checksum:iB3B17644F6EF83CA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti108 – 1081Q → E in AAA39281 (PubMed:3003564).Curated

Polymorphismi

The poly-Gln region is highly polymorphic.Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti26 – 261S → P in strain: RF/J and CAST/Ei.
Natural varianti30 – 301S → P in strain: RF/J.
Natural varianti32 – 398AEAQQQQQ → SSSTAEA in strain: CAST/Ei.
Natural varianti32 – 387AEAQQQQ → SSSTAEA in strain: RF/J.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01663 Genomic DNA. Translation: CAA25823.1.
X01664 Genomic DNA. Translation: CAA25824.1.
X01665 Genomic DNA. Translation: CAA25825.1.
X01772 mRNA. Translation: CAA25909.1.
K02292 mRNA. Translation: AAA39289.1.
M16762, M16760, M16761 Genomic DNA. Translation: AAA39281.1.
AF195956 Genomic DNA. Translation: AAF32272.1.
AF065914 mRNA. Translation: AAD25890.1.
AL662823 Genomic DNA. Translation: CAM18418.1.
X66058 mRNA. Translation: CAA46854.1.
L07574 Genomic DNA. Translation: AAA39326.1.
L07576 Genomic DNA. Translation: AAA39328.1.
CCDSiCCDS17316.1.
PIRiA93550. ICMS2.
I54512.
I68871.
RefSeqiNP_032392.1. NM_008366.3.
UniGeneiMm.14190.

Genome annotation databases

EnsembliENSMUST00000029275; ENSMUSP00000029275; ENSMUSG00000027720.
GeneIDi16183.
KEGGimmu:16183.
UCSCiuc008pai.3. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01663 Genomic DNA. Translation: CAA25823.1.
X01664 Genomic DNA. Translation: CAA25824.1.
X01665 Genomic DNA. Translation: CAA25825.1.
X01772 mRNA. Translation: CAA25909.1.
K02292 mRNA. Translation: AAA39289.1.
M16762, M16760, M16761 Genomic DNA. Translation: AAA39281.1.
AF195956 Genomic DNA. Translation: AAF32272.1.
AF065914 mRNA. Translation: AAD25890.1.
AL662823 Genomic DNA. Translation: CAM18418.1.
X66058 mRNA. Translation: CAA46854.1.
L07574 Genomic DNA. Translation: AAA39326.1.
L07576 Genomic DNA. Translation: AAA39328.1.
CCDSiCCDS17316.1.
PIRiA93550. ICMS2.
I54512.
I68871.
RefSeqiNP_032392.1. NM_008366.3.
UniGeneiMm.14190.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4YQXX-ray2.83A/M47-169[»]
4YUEX-ray2.19C47-169[»]
ProteinModelPortaliP04351.
SMRiP04351. Positions 47-167.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP04351. 1 interaction.
STRINGi10090.ENSMUSP00000029275.

Chemistry

BindingDBiP04351.
ChEMBLiCHEMBL2466.

PTM databases

PhosphoSiteiP04351.

Proteomic databases

PaxDbiP04351.
PRIDEiP04351.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029275; ENSMUSP00000029275; ENSMUSG00000027720.
GeneIDi16183.
KEGGimmu:16183.
UCSCiuc008pai.3. mouse.

Organism-specific databases

CTDi3558.
MGIiMGI:96548. Il2.

Phylogenomic databases

eggNOGiENOG410J049. Eukaryota.
ENOG41119SF. LUCA.
GeneTreeiENSGT00390000003555.
HOGENOMiHOG000048727.
HOVERGENiHBG007496.
InParanoidiP04351.
KOiK05429.
OMAiLMSNINV.
OrthoDBiEOG72RN13.
PhylomeDBiP04351.
TreeFamiTF338200.

Enzyme and pathway databases

ReactomeiR-MMU-114604. GPVI-mediated activation cascade.
R-MMU-392451. G beta:gamma signalling through PI3Kgamma.
R-MMU-451927. Interleukin-2 signaling.
R-MMU-5673001. RAF/MAP kinase cascade.
R-MMU-912526. Interleukin receptor SHC signaling.

Miscellaneous databases

PROiP04351.
SOURCEiSearch...

Gene expression databases

BgeeiP04351.
CleanExiMM_IL2.
GenevisibleiP04351. MM.

Family and domain databases

Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR000779. IL-2.
IPR030477. IL-2_CS.
[Graphical view]
PfamiPF00715. IL2. 1 hit.
[Graphical view]
PRINTSiPR00265. INTERLEUKIN2.
ProDomiPD003649. Interleukin-2. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00189. IL2. 1 hit.
[Graphical view]
SUPFAMiSSF47266. SSF47266. 1 hit.
PROSITEiPS00424. INTERLEUKIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BALB/cJ.
  2. "Unique structure of murine interleukin-2 as deduced from cloned cDNAs."
    Kashima N., Nishi-Takaoka C., Fujita T., Taki S., Yamada G., Hamuro J., Taniguchi T.
    Nature 313:402-404(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: B10BR.
  3. "Use of a cDNA expression vector for isolation of mouse interleukin 2 cDNA clones: expression of T-cell growth-factor activity after transfection of monkey cells."
    Yokota T., Arai N., Lee F., Rennick D., Mosmann T., Arai K.
    Proc. Natl. Acad. Sci. U.S.A. 82:68-72(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
  4. "Cloning and structure of a mouse interleukin-2 chromosomal gene."
    Degrave W., Simons G., Devos R., Plaetinck G., Remaut E., Tavernier J., Fiers W.
    Mol. Biol. Rep. 11:57-61(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "Congenic mapping of the type 1 diabetes locus, Idd3, to a 780-kb region of mouse chromosome 3: identification of a candidate segment of ancestral DNA by haplotype mapping."
    Lyons P.A., Armitage N., Argentina F., Denny P., Hill N.J., Lord C.J., Wilusz M.B., Peterson L.B., Wicker L.S., Todd J.A.
    Genome Res. 10:446-453(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: C57BL/6J.
  6. Ma R.Z., Teuscher C.
    Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
    Tissue: Spleen.
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  8. "A new cDNA sequence for the murine interleukin-2 gene."
    Matesanz F., Alcina A., Pellicer A.
    Biochim. Biophys. Acta 1132:335-336(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-63.
    Strain: RF.
    Tissue: Spleen.
  9. "Existence of at least five interleukin-2 molecules in different mouse strains."
    Matesanz F., Alcina A., Pellicer A.
    Immunogenetics 38:300-303(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-63.
    Strain: CAST/EiJ and RF/J.
    Tissue: Spleen.
  10. "Definition and spatial location of mouse interleukin-2 residues that interact with its heterotrimeric receptor."
    Zurawski S., Vega F. Jr., Doyel E.L., Huyghe B., Flaherty K., McKay D.B., Zurawski G.
    EMBO J. 12:5113-5119(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING.

Entry informationi

Entry nameiIL2_MOUSE
AccessioniPrimary (citable) accession number: P04351
Secondary accession number(s): P97945, Q791T3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: March 20, 1987
Last modified: July 6, 2016
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.