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P04351

- IL2_MOUSE

UniProt

P04351 - IL2_MOUSE

Protein

Interleukin-2

Gene

Il2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.

    GO - Molecular functioni

    1. carbohydrate binding Source: Ensembl
    2. cytokine activity Source: MGI
    3. glycosphingolipid binding Source: Ensembl
    4. interleukin-2 receptor binding Source: MGI

    GO - Biological processi

    1. extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
    2. immune response Source: InterPro
    3. negative regulation of B cell apoptotic process Source: MGI
    4. negative regulation of heart contraction Source: Ensembl
    5. negative regulation of inflammatory response Source: MGI
    6. negative regulation of lymphocyte proliferation Source: MGI
    7. negative regulation of protein phosphorylation Source: Ensembl
    8. positive regulation of activated T cell proliferation Source: MGI
    9. positive regulation of B cell proliferation Source: MGI
    10. positive regulation of cytosolic calcium ion concentration Source: Ensembl
    11. positive regulation of dendritic spine development Source: Ensembl
    12. positive regulation of immunoglobulin secretion Source: MGI
    13. positive regulation of interferon-gamma production Source: MGI
    14. positive regulation of interleukin-17 production Source: Ensembl
    15. positive regulation of isotype switching to IgG isotypes Source: MGI
    16. positive regulation of regulatory T cell differentiation Source: Ensembl
    17. positive regulation of T cell proliferation Source: MGI
    18. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    19. positive regulation of tyrosine phosphorylation of Stat5 protein Source: MGI
    20. protein kinase C-activating G-protein coupled receptor signaling pathway Source: Ensembl
    21. regulation of T cell homeostatic proliferation Source: MGI

    Keywords - Molecular functioni

    Cytokine, Growth factor

    Keywords - Biological processi

    Adaptive immunity, Immunity

    Enzyme and pathway databases

    ReactomeiREACT_210793. Interleukin receptor SHC signaling.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Interleukin-2
    Short name:
    IL-2
    Alternative name(s):
    T-cell growth factor
    Short name:
    TCGF
    Gene namesi
    Name:Il2
    Synonyms:Il-2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:96548. Il2.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: MGI

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Add
    BLAST
    Chaini21 – 169149Interleukin-2PRO_0000015493Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi23 – 231O-linked (GalNAc...)By similarity
    Disulfide bondi92 ↔ 140By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiP04351.

    PTM databases

    PhosphoSiteiP04351.

    Expressioni

    Gene expression databases

    BgeeiP04351.
    CleanExiMM_IL2.
    GenevestigatoriP04351.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Il2rgP349021EBI-80538,EBI-80548

    Protein-protein interaction databases

    IntActiP04351. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP04351.
    SMRiP04351. Positions 21-166.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi35 – 4612Poly-GlnAdd
    BLAST

    Sequence similaritiesi

    Belongs to the IL-2 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG41211.
    GeneTreeiENSGT00390000003555.
    HOGENOMiHOG000048727.
    HOVERGENiHBG007496.
    InParanoidiP04351.
    KOiK05429.
    OMAiKWITFCQ.
    OrthoDBiEOG72RN13.
    PhylomeDBiP04351.
    TreeFamiTF338200.

    Family and domain databases

    Gene3Di1.20.1250.10. 1 hit.
    InterProiIPR009079. 4_helix_cytokine-like_core.
    IPR012351. 4_helix_cytokine_core.
    IPR000779. IL-2.
    [Graphical view]
    PANTHERiPTHR11443. PTHR11443. 1 hit.
    PfamiPF00715. IL2. 1 hit.
    [Graphical view]
    PRINTSiPR00265. INTERLEUKIN2.
    ProDomiPD003649. Interleukin-2. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00189. IL2. 1 hit.
    [Graphical view]
    SUPFAMiSSF47266. SSF47266. 1 hit.
    PROSITEiPS00424. INTERLEUKIN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P04351-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYSMQLASCV TLTLVLLVNS APTSSSTSSS TAEAQQQQQQ QQQQQQHLEQ    50
    LLMDLQELLS RMENYRNLKL PRMLTFKFYL PKQATELKDL QCLEDELGPL 100
    RHVLDLTQSK SFQLEDAENF ISNIRVTVVK LKGSDNTFEC QFDDESATVV 150
    DFLRRWIAFC QSIISTSPQ 169
    Length:169
    Mass (Da):19,400
    Last modified:March 20, 1987 - v1
    Checksum:iB3B17644F6EF83CA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti108 – 1081Q → E in AAA39281. (PubMed:3003564)Curated

    Polymorphismi

    The poly-Gln region is highly polymorphic.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti26 – 261S → P in strain: RF/J and CAST/Ei.
    Natural varianti30 – 301S → P in strain: RF/J.
    Natural varianti32 – 398AEAQQQQQ → SSSTAEA in strain: CAST/Ei.
    Natural varianti32 – 387AEAQQQQ → SSSTAEA in strain: RF/J.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X01663 Genomic DNA. Translation: CAA25823.1.
    X01664 Genomic DNA. Translation: CAA25824.1.
    X01665 Genomic DNA. Translation: CAA25825.1.
    X01772 mRNA. Translation: CAA25909.1.
    K02292 mRNA. Translation: AAA39289.1.
    M16762, M16760, M16761 Genomic DNA. Translation: AAA39281.1.
    AF195956 Genomic DNA. Translation: AAF32272.1.
    AF065914 mRNA. Translation: AAD25890.1.
    AL662823 Genomic DNA. Translation: CAM18418.1.
    X66058 mRNA. Translation: CAA46854.1.
    L07574 Genomic DNA. Translation: AAA39326.1.
    L07576 Genomic DNA. Translation: AAA39328.1.
    CCDSiCCDS17316.1.
    PIRiA93550. ICMS2.
    I54512.
    I68871.
    RefSeqiNP_032392.1. NM_008366.3.
    UniGeneiMm.14190.

    Genome annotation databases

    EnsembliENSMUST00000029275; ENSMUSP00000029275; ENSMUSG00000027720.
    GeneIDi16183.
    KEGGimmu:16183.
    UCSCiuc008pai.3. mouse.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X01663 Genomic DNA. Translation: CAA25823.1 .
    X01664 Genomic DNA. Translation: CAA25824.1 .
    X01665 Genomic DNA. Translation: CAA25825.1 .
    X01772 mRNA. Translation: CAA25909.1 .
    K02292 mRNA. Translation: AAA39289.1 .
    M16762 , M16760 , M16761 Genomic DNA. Translation: AAA39281.1 .
    AF195956 Genomic DNA. Translation: AAF32272.1 .
    AF065914 mRNA. Translation: AAD25890.1 .
    AL662823 Genomic DNA. Translation: CAM18418.1 .
    X66058 mRNA. Translation: CAA46854.1 .
    L07574 Genomic DNA. Translation: AAA39326.1 .
    L07576 Genomic DNA. Translation: AAA39328.1 .
    CCDSi CCDS17316.1.
    PIRi A93550. ICMS2.
    I54512.
    I68871.
    RefSeqi NP_032392.1. NM_008366.3.
    UniGenei Mm.14190.

    3D structure databases

    ProteinModelPortali P04351.
    SMRi P04351. Positions 21-166.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P04351. 1 interaction.

    Chemistry

    BindingDBi P04351.
    ChEMBLi CHEMBL2466.

    PTM databases

    PhosphoSitei P04351.

    Proteomic databases

    PRIDEi P04351.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000029275 ; ENSMUSP00000029275 ; ENSMUSG00000027720 .
    GeneIDi 16183.
    KEGGi mmu:16183.
    UCSCi uc008pai.3. mouse.

    Organism-specific databases

    CTDi 3558.
    MGIi MGI:96548. Il2.

    Phylogenomic databases

    eggNOGi NOG41211.
    GeneTreei ENSGT00390000003555.
    HOGENOMi HOG000048727.
    HOVERGENi HBG007496.
    InParanoidi P04351.
    KOi K05429.
    OMAi KWITFCQ.
    OrthoDBi EOG72RN13.
    PhylomeDBi P04351.
    TreeFami TF338200.

    Enzyme and pathway databases

    Reactomei REACT_210793. Interleukin receptor SHC signaling.

    Miscellaneous databases

    NextBioi 289069.
    PROi P04351.
    SOURCEi Search...

    Gene expression databases

    Bgeei P04351.
    CleanExi MM_IL2.
    Genevestigatori P04351.

    Family and domain databases

    Gene3Di 1.20.1250.10. 1 hit.
    InterProi IPR009079. 4_helix_cytokine-like_core.
    IPR012351. 4_helix_cytokine_core.
    IPR000779. IL-2.
    [Graphical view ]
    PANTHERi PTHR11443. PTHR11443. 1 hit.
    Pfami PF00715. IL2. 1 hit.
    [Graphical view ]
    PRINTSi PR00265. INTERLEUKIN2.
    ProDomi PD003649. Interleukin-2. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00189. IL2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47266. SSF47266. 1 hit.
    PROSITEi PS00424. INTERLEUKIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: BALB/c.
    2. "Unique structure of murine interleukin-2 as deduced from cloned cDNAs."
      Kashima N., Nishi-Takaoka C., Fujita T., Taki S., Yamada G., Hamuro J., Taniguchi T.
      Nature 313:402-404(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: B10BR.
    3. "Use of a cDNA expression vector for isolation of mouse interleukin 2 cDNA clones: expression of T-cell growth-factor activity after transfection of monkey cells."
      Yokota T., Arai N., Lee F., Rennick D., Mosmann T., Arai K.
      Proc. Natl. Acad. Sci. U.S.A. 82:68-72(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
    4. "Cloning and structure of a mouse interleukin-2 chromosomal gene."
      Degrave W., Simons G., Devos R., Plaetinck G., Remaut E., Tavernier J., Fiers W.
      Mol. Biol. Rep. 11:57-61(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    5. "Congenic mapping of the type 1 diabetes locus, Idd3, to a 780-kb region of mouse chromosome 3: identification of a candidate segment of ancestral DNA by haplotype mapping."
      Lyons P.A., Armitage N., Argentina F., Denny P., Hill N.J., Lord C.J., Wilusz M.B., Peterson L.B., Wicker L.S., Todd J.A.
      Genome Res. 10:446-453(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: C57BL/6.
    6. Ma R.Z., Teuscher C.
      Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6J.
      Tissue: Spleen.
    7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    8. "A new cDNA sequence for the murine interleukin-2 gene."
      Matesanz F., Alcina A., Pellicer A.
      Biochim. Biophys. Acta 1132:335-336(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-63.
      Strain: RF.
      Tissue: Spleen.
    9. "Existence of at least five interleukin-2 molecules in different mouse strains."
      Matesanz F., Alcina A., Pellicer A.
      Immunogenetics 38:300-303(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-63.
      Strain: CAST/Ei and RF/J.
      Tissue: Spleen.
    10. "Definition and spatial location of mouse interleukin-2 residues that interact with its heterotrimeric receptor."
      Zurawski S., Vega F. Jr., Doyel E.L., Huyghe B., Flaherty K., McKay D.B., Zurawski G.
      EMBO J. 12:5113-5119(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING.

    Entry informationi

    Entry nameiIL2_MOUSE
    AccessioniPrimary (citable) accession number: P04351
    Secondary accession number(s): P97945, Q791T3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 20, 1987
    Last sequence update: March 20, 1987
    Last modified: October 1, 2014
    This is version 133 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3