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Protein

Portal protein

Gene

10

Organism
Bacillus phage phi29 (Bacteriophage phi-29)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Forms the portal vertex of the capsid (PubMed:10801350) (PubMed:19744688, PubMed:21570409). This portal plays critical roles in head assembly, genome packaging, neck/tail attachment, and genome ejection (By similarity). The portal protein multimerizes as a single ring-shaped homododecamer arranged around a central channel (PubMed:11812138, PubMed:21570409). Binds to the 6 packaging RNA molecules (pRNA) forming a double-ring structure which in turn binds to the ATPase gp16 hexamer, forming the active DNA-translocating motor (PubMed:15886394, PubMed:11130079). This complex is essential for the specificity of packaging from the left DNA end.By similarity1 Publication5 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Viral short tail ejection system

Keywords - Biological processi

Viral capsid assembly, Viral genome ejection through host cell envelope, Viral genome packaging, Viral penetration into host cytoplasm, Virus entry into host cell, Virus exit from host cell

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Portal proteinCurated
Alternative name(s):
Connector protein1 Publication
Gene product 10Curated
Short name:
gp10Curated
Gene product 19
Short name:
gp19
Head-to-tail connector1 Publication
Probable portal protein
Protein p10Curated
Upper collar proteinCurated
Gene namesi
Name:10
OrganismiBacillus phage phi29 (Bacteriophage phi-29)
Taxonomic identifieri10756 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaePicovirinaePhi29likevirus
Virus hostiBacillus subtilis [TaxID: 1423]
Proteomesi
  • UP000001207 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

  • viral portal complex Source: UniProtKB
  • viral procapsid Source: CACAO
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001065861 – 309Portal proteinAdd BLAST309

Expressioni

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Homododecamer (PubMed:11130079, PubMed:11526317) (PubMed:11812138). Interacts (via N-terminus) with the pRNA (PubMed:15886394).4 Publications

Structurei

Secondary structure

1309
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi14 – 16Combined sources3
Helixi18 – 36Combined sources19
Beta strandi38 – 43Combined sources6
Helixi50 – 60Combined sources11
Beta strandi61 – 68Combined sources8
Turni69 – 71Combined sources3
Beta strandi72 – 76Combined sources5
Beta strandi78 – 82Combined sources5
Beta strandi86 – 88Combined sources3
Beta strandi92 – 96Combined sources5
Beta strandi97 – 99Combined sources3
Beta strandi101 – 106Combined sources6
Beta strandi110 – 112Combined sources3
Beta strandi118 – 124Combined sources7
Helixi131 – 154Combined sources24
Beta strandi159 – 163Combined sources5
Helixi166 – 168Combined sources3
Helixi171 – 176Combined sources6
Beta strandi180 – 183Combined sources4
Beta strandi195 – 200Combined sources6
Helixi207 – 224Combined sources18
Helixi247 – 272Combined sources26
Beta strandi277 – 281Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FOUX-ray3.20A/B/C/D/E/F/G/H/I/J/K/L1-309[»]
1H5WX-ray2.10A/B/C1-309[»]
1IJGX-ray2.90A/B/C/D/E/F/G/H/I/J/K/L1-309[»]
1JNBX-ray3.20A/B/C/D/E/F/G/H/I/J/K/L1-309[»]
1L4Pmodel-G/H/I/J/K/L/M/N/O/P/Q/R1-309[»]
1VRImodel-1/2/3/4/5/6/7/8/A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d/e/f/g/h/i/j/k/l/m/n/o/p/q/r/s/t/u/v/w/x/y/z1-309[»]
1VRJmodel-A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d/e/f/g/h/i/j1-309[»]
1YWEmodel-1/2/3/4/5/6/7/8/A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d/e/f/g/h/i/j/k/l/m/n/o/p/q/r/s/t/u/v/w/x/y/z1-309[»]
ProteinModelPortaliP04332.
SMRiP04332.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04332.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 14Binding to the pRNA1 PublicationAdd BLAST14

Sequence similaritiesi

Family and domain databases

InterProiIPR008016. Gp10.
[Graphical view]
PfamiPF05352. Phage_connector. 1 hit.
[Graphical view]
ProDomiPD017810. Upper_collar_prot_phg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF56826. SSF56826. 1 hit.

Sequencei

Sequence statusi: Complete.

P04332-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARKRSNTYR SINEIQRQKR NRWFIHYLNY LQSLAYQLFE WENLPPTINP
60 70 80 90 100
SFLEKSIHQF GYVGFYKDPV ISYIACNGAL SGQRDVYNQA TVFRAASPVY
110 120 130 140 150
QKEFKLYNYR DMKEEDMGVV IYNNDMAFPT TPTLELFAAE LAELKEIISV
160 170 180 190 200
NQNAQKTPVL IRANDNNQLS LKQVYNQYEG NAPVIFAHEA LDSDSIEVFK
210 220 230 240 250
TDAPYVVDKL NAQKNAVWNE MMTFLGIKNA NLEKKERMVT DEVSSNDEQI
260 270 280 290 300
ESSGTVFLKS REEACEKINE LYGLNVKVKF RYDIVEQMRR ELQQIENVSR

GTSDGETNE
Length:309
Mass (Da):35,878
Last modified:March 20, 1987 - v1
Checksum:iF5B01C1BE17FDE60
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14782 Genomic DNA. Translation: AAA32283.1.
M12456 Genomic DNA. Translation: AAA32292.1.
EU771092 Genomic DNA. Translation: ACE96033.1.
PIRiE25816. WMBPC9.
RefSeqiYP_002004539.1. NC_011048.1.

Genome annotation databases

GeneIDi6446518.
KEGGivg:6446518.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14782 Genomic DNA. Translation: AAA32283.1.
M12456 Genomic DNA. Translation: AAA32292.1.
EU771092 Genomic DNA. Translation: ACE96033.1.
PIRiE25816. WMBPC9.
RefSeqiYP_002004539.1. NC_011048.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FOUX-ray3.20A/B/C/D/E/F/G/H/I/J/K/L1-309[»]
1H5WX-ray2.10A/B/C1-309[»]
1IJGX-ray2.90A/B/C/D/E/F/G/H/I/J/K/L1-309[»]
1JNBX-ray3.20A/B/C/D/E/F/G/H/I/J/K/L1-309[»]
1L4Pmodel-G/H/I/J/K/L/M/N/O/P/Q/R1-309[»]
1VRImodel-1/2/3/4/5/6/7/8/A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d/e/f/g/h/i/j/k/l/m/n/o/p/q/r/s/t/u/v/w/x/y/z1-309[»]
1VRJmodel-A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d/e/f/g/h/i/j1-309[»]
1YWEmodel-1/2/3/4/5/6/7/8/A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d/e/f/g/h/i/j/k/l/m/n/o/p/q/r/s/t/u/v/w/x/y/z1-309[»]
ProteinModelPortaliP04332.
SMRiP04332.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi6446518.
KEGGivg:6446518.

Miscellaneous databases

EvolutionaryTraceiP04332.

Family and domain databases

InterProiIPR008016. Gp10.
[Graphical view]
PfamiPF05352. Phage_connector. 1 hit.
[Graphical view]
ProDomiPD017810. Upper_collar_prot_phg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF56826. SSF56826. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPORTL_BPPH2
AccessioniPrimary (citable) accession number: P04332
Secondary accession number(s): B3VMP6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: March 20, 1987
Last modified: November 2, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.