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Protein

Outer capsid glycoprotein VP7

Gene
N/A
Organism
Rotavirus A (isolate Human/Australia/Hu5/1977 G2-Px[x]-Ix-Rx-Cx-Mx-Ax-Nx-Tx-Ex-Hx) (RV-A) (Rotavirus A (isolate Hu/5))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Outer capsid protein involved in attachment and possibly entry into the host epithelial cell. It is subsequently lost, together with VP4, following virus entry into the host cell. The outer layer contains 780 copies of VP7, grouped as 260 trimers. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. In integrin-dependent strains, VP7 seems to essentially target the integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3 at a postbinding stage, once the initial attachment by VP4 has been achieved (By similarity).By similarity

GO - Biological processi

  1. viral process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Outer capsid glycoprotein VP7
OrganismiRotavirus A (isolate Human/Australia/Hu5/1977 G2-Px[x]-Ix-Rx-Cx-Mx-Ax-Nx-Tx-Ex-Hx) (RV-A) (Rotavirus A (isolate Hu/5))
Taxonomic identifieri10948 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiHomo sapiens (Human) [TaxID: 9606]

Subcellular locationi

Virion By similarity. Host endoplasmic reticulum lumen Curated
Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles (By similarity).By similarity

GO - Cellular componenti

  1. host cell endoplasmic reticulum lumen Source: UniProtKB-SubCell
  2. T=13 icosahedral viral capsid Source: UniProtKB-KW
  3. viral outer capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host endoplasmic reticulum, Outer capsid protein, T=13 icosahedral capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 5050Sequence AnalysisAdd
BLAST
Chaini51 – 326276Outer capsid glycoprotein VP7Sequence AnalysisPRO_0000149595Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi69 – 691N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi146 – 1461N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi238 – 2381N-linked (GlcNAc...); by hostSequence Analysis

Post-translational modificationi

The N-terminus is blocked possibly by pyroglutamic acid.By similarity
N-glycosylated.By similarity
Intramolecular disulfide bonds.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homotrimer; in the presence of calcium (By similarity). Acquisition of the capsid outer layer requires a high calcium concentration inside the endoplasmic reticulum. Following cell entry, the low calcium concentration in the cytoplasm is probably responsible for the solubilization of the outer layer. Interacts with host integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3 (By similarity).By similarity

Family & Domainsi

Sequence similaritiesi

Belongs to the rotavirus VP7 family.Curated

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR001963. VP7.
[Graphical view]
PfamiPF00434. VP7. 1 hit.
[Graphical view]
ProDomiPD000191. VP7. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 1 (identifier: P04328-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYGIEYTTIL TILISIILLN YILKTITNTM DYIIFRFLLL IALISPFVRT
60 70 80 90 100
QNYGMYLPIT GSLDAVYTNS TSGEPFLTST LCLYYPAEAK NEISDDEWEN
110 120 130 140 150
TLSQLFLTKG WPIGSVYFKD YNDINTFSVN PQLYCDYNVV LMRYDNTSEL
160 170 180 190 200
DASELADLIL NEWLCNPMDI SLYYYQQSSE SNKWISMGTD CTVKVCPLNT
210 220 230 240 250
QTLGIGCKTT DVNTFEIVAS SEKLVITDVV NGVNHNINIS INTCTIRNCN
260 270 280 290 300
KLGPRENVAI IQVGGPNALD ITADPTTVPQ VQRIMRINWK KWWQVFYTVV
310 320
DYINQVIQVM SKRSRSLDAA AFYYRI
Length:326
Mass (Da):37,203
Last modified:March 19, 1987 - v1
Checksum:i2D438E6EEC86FCB0
GO
Isoform 2 (identifier: P04328-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: Missing.

Note: Produced by alternative initiation at Met-30 of isoform 1. No experimental confirmation available.

Show »
Length:297
Mass (Da):33,875
Checksum:i0D830F4CA983E543
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2929Missing in isoform 2. CuratedVSP_038594Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00572 mRNA. Translation: CAA25236.1.
A01028 Unassigned RNA. Translation: CAA00124.1.
PIRiG27620. VGXRHU.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00572 mRNA. Translation: CAA25236.1.
A01028 Unassigned RNA. Translation: CAA00124.1.
PIRiG27620. VGXRHU.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR001963. VP7.
[Graphical view]
PfamiPF00434. VP7. 1 hit.
[Graphical view]
ProDomiPD000191. VP7. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Publicationsi

  1. "Sequence homology between human and animal rotavirus serotype-specific glycoproteins."
    Dyall-Smith M.L., Holmes I.H.
    Nucleic Acids Res. 12:3973-3982(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Comparison of the amino acid sequences of the major neutralization protein of four human rotavirus serotypes."
    Green K.Y., Midthun K., Gorziglia M., Hoshino Y., Kapikian A.Z., Chanock R.M., Flores J.
    Virology 161:153-159(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiVP7_ROTHA
AccessioniPrimary (citable) accession number: P04328
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 19, 1987
Last sequence update: March 19, 1987
Last modified: January 6, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

In group A rotaviruses, VP7 defines the G serotype.

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.