P04323 (POL3_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Retrovirus-related Pol polyprotein from transposon 17.6

Including the following 3 domains:

1. Protease
   EC=3.4.23.-
2. Reverse transcriptase
   EC=2.7.7.49
3. Endonuclease

Gene names

Name:

pol

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1058 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Miscellaneous	The open reading frame is located in a copia-like transposable element called 17.6.
Sequence similarities	Contains 1 integrase catalytic domain. Contains 1 peptidase A2 domain. Contains 1 reverse transcriptase domain.

Ontologies

Keywords
Molecular function	Aspartyl protease Endonuclease Hydrolase Nuclease Nucleotidyltransferase Protease RNA-directed DNA polymerase Transferase
Technical term	Transposable element
Gene Ontology (GO)
Biological process	DNA integration Inferred from electronic annotation. Source: InterPro RNA-dependent DNA replication Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1058	1058	Retrovirus-related Pol polyprotein from transposon 17.6		PRO_0000199557
Regions
	Domain	231 – 415	185	Reverse transcriptase
	Domain	810 – 967	158	Integrase catalytic
Sites
	Active site	30	1	For protease activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P04323 [UniParc]. Last modified March 20, 1987. Version 1. Checksum: C893F5C4A7E1F091	FASTA	1,058	122,698
	10 20 30 40 50 60 TGRKFSATSL GKPQYITIKY KENNLKCLID TGSTVNMTSK NIFDLPIQNT STFIHTSNGP 70 80 90 100 110 120 LIVNKSIIIP SKILFPTTNE FLLHPFSENY DLLLGRKLLA EAKATISYRD QEVTLYNNKY 130 140 150 160 170 180 KLIEGIATHE QSHFQNVNMI PDTMLRQPNK ISPILESDLY RLEHLNNEEK QRLCALLQKY 190 200 210 220 230 240 HDIQYHEGDK LTFTNQTKHT INTKHNLPLY SKYSYPQAYE QEVESQIQDM LNQGIIRTSN 250 260 270 280 290 300 SPYNSPIWVV PKKQDASGKQ KFRIVIDYRK LNEITVGDRH PIPNMDEILG KLGRCNYFTT 310 320 330 340 350 360 IDLAKGFHQI EMDPESVSKT AFSTKHGHYE YLRMPFGLKN APATFQRCMN DILRPLLNKH 370 380 390 400 410 420 CLVYLDDIIV FSTSLDEHLQ SLGLVFEKLA KANLKLQLDK CEFLKQETTF LGHVLTPDGI 430 440 450 460 470 480 KPNPEKIEAI QKYPIPTKPK EIKAFLGLTG YYRKFIPNFA DIAKPMTKCL KKNMKIDTTN 490 500 510 520 530 540 PEYDSAFKKL KYLISEDPIL KVPDFTKKFT LTTDASDVAL GAVLSQDGHP LSYISRTLNE 550 560 570 580 590 600 HEINYSTIEK ELLAIVWATK TFRHYLLGRH FEISSDHQPL SWLYRMKDPN SKLTRWRVKL 610 620 630 640 650 660 SEFDFDIKYI KGKENCVADA LSRIKLEETY LSEQTQHSAE EDNSDLIFIT ERPLNTFNRQ 670 680 690 700 710 720 VIFSKGPPDI KVTKYFKKHI TQIFYDIMTR EKAEQYLIDH FCGKKSALYI ESDADFEVIQ 730 740 750 760 770 780 AAHKLAINTK YTKILRSTIL LKNITTYAEF KELILTAHEK LLHPGIQKTT KLFGETYYFP 790 800 810 820 830 840 NSQLLIQNII NECSICNLAK TEHRNTDMPT KTTPKPEHCR EKFMIDIYSS EGKHYVSCID 850 860 870 880 890 900 IYSKFATLEE IKTKDWIECK NALMRIFNQL GKPKLLKADR DGAFSSLALK RWLESEEVEL 910 920 930 940 950 960 QLNTTKTGVA DIERLHKTIN EKIRIIKTSD DEETKLSKME TVLNIYNHKT KHDTTGQTPA 970 980 990 1000 1010 1020 HIFLYAGQPI LDTQQNKENK INKINNDRVE YEVDTRYRKG PLQKGKLENP FKPTKNVEQT 1030 1040 1050 DSDHYKITNR NRITHYYKTQ FKKRKKNNQL SISQAPGT

References

[1]	"Identification of the coding sequence for a reverse transcriptase-like enzyme in a transposable genetic element in Drosophila melanogaster." Saigo K., Kugimiya W., Matsuo Y., Inouye S., Yoshioka K., Yuki S. Nature 312:659-661(1984) [PubMed: 6209583] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X01472 Genomic DNA. Translation: CAA25702.1.
PIR	GNFF17. A03971.
3D structure databases
ProteinModelPortal	P04323.
ModBase	Search...
Protein family/group databases
MEROPS	A02.052.
Proteomic databases
PRIDE	P04323.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
FlyBase	FBgn0014453. 17.6\pol.
Family and domain databases
InterPro	IPR001584. Integrase_cat-core. IPR018061. Pept_A2A_retrovirus_sg. IPR021109. Peptidase_aspartic. IPR012337. RNaseH-like_dom. IPR000477. RVT. [Graphical view]
Pfam	PF00665. rve. 1 hit. PF00077. RVP. 1 hit. PF00078. RVT_1. 1 hit. [Graphical view]
SUPFAM	SSF50630. Pept_Aspartic. 1 hit. SSF53098. RNaseH_fold. 1 hit.
PROSITE	PS00141. ASP_PROTEASE. 1 hit. PS50994. INTEGRASE. 1 hit. PS50878. RT_POL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POL3_DROME

Accession

Primary (citable) accession number: P04323

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 1987
Last sequence update:	March 20, 1987
Last modified:	December 14, 2011
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project