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Protein

mRNA-capping enzyme regulatory subunit

Gene

VACWR117

Organism
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit of the mRNA cap enzyme which stabilizes the catalytic subunit and enhances its methyltransferase activity through an allosteric mechanism. Heterodimeric mRNA capping enzyme catalyzes the linkage of a N7-methyl-guanosine moiety to the first transcribed nucleotide (cap 0 structure), whereas the polymerase associated VP39 is responsible for a second methylation at the 2'-O position of the ribose (cap 1 structure).
The heterodimeric enzyme is also involved in early viral gene transcription termination and intermediate viral gene transcription initiation. Early gene transcription termination requires the termination factor VTF, the DNA-dependent ATPase NPH-I and the Rap94 subunit of the viral RNA polymerase, as well as the presence of a specific termination motif. Binds, together with RAP94, to the termination motif 5'-UUUUUNU-3' in the nascent early mRNA.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processmRNA capping, mRNA processing, Transcription, Transcription regulation, Transcription termination

Names & Taxonomyi

Protein namesi
Recommended name:
mRNA-capping enzyme regulatory subunit
Alternative name(s):
Virus termination factor small subunit
Short name:
VTF small subunit
mRNA-capping enzyme 33 kDa subunit
mRNA-capping enzyme D12 subunit
mRNA-capping enzyme small subunit
Gene namesi
Ordered Locus Names:VACWR117
ORF Names:D12L
OrganismiVaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Taxonomic identifieri10254 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostiBos taurus (Bovine) [TaxID: 9913]
Proteomesi
  • UP000000344 Componenti: Genome

Subcellular locationi

  • Virion Curated

  • Note: All the enzymes and other proteins required to synthesize early mRNAs are packaged within the virion core along with the DNA genome.

GO - Cellular componenti

  • virion Source: UniProtKB-SubCell

Keywords - Cellular componenti

Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi23P → S in ts9383; conditional lethal mutant defective in the ability to convert the replicated form of the viral telomere to hairpin termini. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002101371 – 287mRNA-capping enzyme regulatory subunitAdd BLAST287

Interactioni

Subunit structurei

Heterodimer of a catalytic and a regulatory subunit. Intrinsic methyltransferase activity of the catalytic subunit is weak and needs to be stimulated 30- to 50-fold by the regulatory subunit, which is itself catalytically inert.1 Publication

Protein-protein interaction databases

DIPiDIP-60665N.

Structurei

Secondary structure

1287
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 10Combined sources8
Beta strandi12 – 15Combined sources4
Beta strandi28 – 30Combined sources3
Turni37 – 40Combined sources4
Helixi43 – 46Combined sources4
Helixi48 – 54Combined sources7
Helixi58 – 65Combined sources8
Helixi66 – 68Combined sources3
Helixi74 – 81Combined sources8
Beta strandi87 – 90Combined sources4
Beta strandi97 – 102Combined sources6
Beta strandi110 – 112Combined sources3
Beta strandi128 – 131Combined sources4
Helixi133 – 135Combined sources3
Helixi138 – 141Combined sources4
Helixi142 – 146Combined sources5
Beta strandi151 – 158Combined sources8
Helixi163 – 175Combined sources13
Beta strandi176 – 183Combined sources8
Beta strandi189 – 191Combined sources3
Beta strandi194 – 201Combined sources8
Helixi203 – 217Combined sources15
Helixi224 – 226Combined sources3
Beta strandi230 – 235Combined sources6
Helixi239 – 268Combined sources30
Beta strandi273 – 276Combined sources4
Helixi277 – 283Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VDWX-ray2.70B/D/F/H1-287[»]
4CKBX-ray2.80B/E1-287[»]
4CKCX-ray2.90B/E1-287[»]
4CKEX-ray2.90B/E1-287[»]
ProteinModelPortaliP04318.
SMRiP04318.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04318.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

OrthoDBiVOG090000HH.

Family and domain databases

InterProiView protein in InterPro
IPR005009. Poxvirus_mRNA-cap_ssu.
PfamiView protein in Pfam
PF03341. Pox_mRNA-cap. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD009934. Pox_mRNA-cap. 1 hit.

Sequencei

Sequence statusi: Complete.

P04318-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDEIVKNIRE GTHVLLPFYE TLPELNLSLG KSPLPSLEYG ANYFLQISRV
60 70 80 90 100
NDLNRMPTDM LKLFTHDIML PESDLDKVYE ILKINSVKYY GRSTKADAVV
110 120 130 140 150
ADLSARNKLF KRERDAIKSN NHLTENNLYI SDYKMLTFDV FRPLFDFVNE
160 170 180 190 200
KYCIIKLPTL FGRGVIDTMR IYCSLFKNVR LLKCVSDSWL KDSAIMVASD
210 220 230 240 250
VCKKNLDLFM SHVKSVTKSS SWKDVNSVQF SILNNPVDTE FINKFLEFSN
260 270 280
RVYEALYYVH SLLYSSMTSD SKSIENKHQR RLVKLLL
Length:287
Mass (Da):33,352
Last modified:March 20, 1987 - v1
Checksum:iEA9CE30A7661A7ED
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti134K → N in CAA27369 (PubMed:3008103).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15058 Genomic DNA. Translation: AAA48270.1.
X03729 Genomic DNA. Translation: CAA27369.1.
AY243312 Genomic DNA. Translation: AAO89396.1.
PIRiA03892. QQVZ22.
RefSeqiYP_232999.1. NC_006998.1.

Genome annotation databases

GeneIDi3707515.
KEGGivg:3707515.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15058 Genomic DNA. Translation: AAA48270.1.
X03729 Genomic DNA. Translation: CAA27369.1.
AY243312 Genomic DNA. Translation: AAO89396.1.
PIRiA03892. QQVZ22.
RefSeqiYP_232999.1. NC_006998.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VDWX-ray2.70B/D/F/H1-287[»]
4CKBX-ray2.80B/E1-287[»]
4CKCX-ray2.90B/E1-287[»]
4CKEX-ray2.90B/E1-287[»]
ProteinModelPortaliP04318.
SMRiP04318.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60665N.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3707515.
KEGGivg:3707515.

Phylogenomic databases

OrthoDBiVOG090000HH.

Miscellaneous databases

EvolutionaryTraceiP04318.

Family and domain databases

InterProiView protein in InterPro
IPR005009. Poxvirus_mRNA-cap_ssu.
PfamiView protein in Pfam
PF03341. Pox_mRNA-cap. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD009934. Pox_mRNA-cap. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMCES_VACCW
AccessioniPrimary (citable) accession number: P04318
Secondary accession number(s): Q76ZR5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: March 20, 1987
Last modified: February 15, 2017
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.