Reviewed, UniProtKB/Swiss-Prot P04298 (MCEL_VACCW)

Names and origin

Protein names

Recommended name:
    mRNA-capping enzyme large subunit
Including the following 2 domains:
    1- Recommended name:
            Polynucleotide 5'-triphosphatase
              EC=3.1.3.33
        Alternative name(s):
            mRNA 5'-triphosphatase
              Short name=TPase
    2- Recommended name:
            mRNA guanylyltransferase
              EC=2.7.7.50
        Alternative name(s):
            GTP--RNA guanylyltransferase
              Short name=GTase

Gene names

Ordered Locus Names:	VACWR106
ORF Names:	D1R

Organism

Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))

Taxonomic identifier

10254 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus › Vaccinia virus

Virus host

Bos taurus (Bovine) [TaxID: 9913]

Protein attributes

Sequence length	844 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the first two reactions in the mRNA cap formation pathway.
Catalytic activity	A 5'-phosphopolynucleotide + H2O = a polynucleotide + phosphate. GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
Subunit structure	Heterodimer of a large and a small subunit. Ref.3
Sequence similarities	Belongs to the viral GTase family.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Ligand	GTP-binding Nucleotide-binding
Molecular function	Hydrolase Nucleotidyltransferase Transferase
Technical term	3D-structure Direct protein sequencing Multifunctional enzyme
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW mRNA guanylyltransferase activity Inferred from electronic annotation. Source: EC polynucleotide 5'-phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 844

844

mRNA-capping enzyme large subunit

PRO_0000210124

Sites

Active site

260

1

N6-GMP-lysine intermediate Potential

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

844

Helix Strand Turn

Details...

Helix

563 – 580

18

Turn

583 – 585

3

Beta strand

593 – 596

4

Turn

600 – 604

5

Helix

605 – 610

6

Beta strand

614 – 621

8

Helix

623 – 636

14

Beta strand

646 – 651

6

Beta strand

656 – 658

3

Helix

659 – 664

6

Beta strand

672 – 679

8

Helix

681 – 683

3

Turn

687 – 689

3

Helix

690 – 700

11

Beta strand

701 – 712

12

Helix

714 – 717

4

Beta strand

724 – 727

4

Beta strand

730 – 732

3

Turn

734 – 736

3

Beta strand

737 – 741

5

Beta strand

743 – 745

3

Beta strand

748 – 752

5

Turn

754 – 756

3

Beta strand

757 – 759

3

Beta strand

761 – 764

4

Helix

768 – 777

10

Beta strand

780 – 787

8

Helix

788 – 793

6

Helix

796 – 800

5

Helix

802 – 805

4

Helix

809 – 823

15

Helix

829 – 833

5

Beta strand

836 – 843

8

Sequences

	Sequence		Length	Mass (Da)
	P04298-1 [UniParc]. Last modified March 20, 1987. Version 1. Checksum: 8B9FD3DE836FA6E7	FASTA	844	96,734
	10 20 30 40 50 60 MDANVVSSST IATYIDALAK NASELEQRST AYEINNELEL VFIKPPLITL TNVVNISTIQ 70 80 90 100 110 120 ESFIRFTVTN KEGVKIRTKI PLSKVHGLDV KNVQLVDAID NIVWEKKSLV TENRLHKECL 130 140 150 160 170 180 LRLSTEERHI FLDYKKYGSS IRLELVNLIQ AKTKNFTIDF KLKYFLGSGA QSKSSLLHAI 190 200 210 220 230 240 NHPKSRPNTS LEIEFTPRDN ETVPYDELIK ELTTLSRHIF MASPENVILS PPINAPIKTF 250 260 270 280 290 300 MLPKQDIVGL DLENLYAVTK TDGIPITIRV TSNGLYCYFT HLGYIIRYPV KRIIDSEVVV 310 320 330 340 350 360 FGEAVKDKNW TVYLIKLIEP VNAINDRLEE SKYVESKLVD ICDRIVFKSK KYEGPFTTTS 370 380 390 400 410 420 EVVDMLSTYL PKQPEGVILF YSKGPKSNID FKIKKENTID QTANVVFRYM SSEPIIFGES 430 440 450 460 470 480 SIFVEYKKFS NDKGFPKEYG SGKIVLYNGV NYLNNIYCLE YINTHNEVGI KSVVVPIKFI 490 500 510 520 530 540 AEFLVNGEIL KPRIDKTMKY INSEDYYGNQ HNIIVEHLRD QSIKIGDIFN EDKLSDVGHQ 550 560 570 580 590 600 YANNDKFRLN PEVSYFTNKR TRGPLGILSN YVKTLLISMY CSKTFLDDSN KRKVLAIDFG 610 620 630 640 650 660 NGADLEKYFY GEIALLVATD PDADAIARGN ERYNKLNSGI KTKYYKFDYI QETIRSDTFV 670 680 690 700 710 720 SSVREVFYFG KFNIIDWQFA IHYSFHPRHY ATVMNNLSEL TASGGKVLIT TMDGDKLSKL 730 740 750 760 770 780 TDKKTFIIHK NLPSSENYMS VEKIADDRIV VYNPSTMSTP MTEYIIKKND IVRVFNEYGF 790 800 810 820 830 840 VLVDNVDFAT IIERSKKFIN GASTMEDRPS TRNFFELNRG AIKCEGLDVE DLLSYYVVYV FSKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence and genetic map of the 16-kb vaccinia virus HindIII D fragment." Niles E.G., Condit R.C., Caro P., Davidson K., Matusick L., Seto J. Virology 153:96-112(1986) [PubMed: 3739227] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb." Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Interaction and mutual stabilization of the two subunits of vaccinia virus mRNA capping enzyme coexpressed in Escherichia coli." Guo P., Moss B. Proc. Natl. Acad. Sci. U.S.A. 87:4023-4027(1990) [PubMed: 2161527] [Abstract] Cited for: INTERACTION WITH SMALL SUBUNIT, PROTEIN SEQUENCE OF 1-20 AND 498-517.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M15058 Genomic DNA. Translation: AAA48253.1.
AY243312 Genomic DNA. Translation: AAO89385.1.

PIR

QQVZ1. A03872.

RefSeq

YP_232988.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2VDW	X-ray	2.70	A/C/E/G	545-844	[»]

ModBase

Search...

Genome annotation databases

GeneID

3707562.

Family and domain databases

InterPro

IPR019602. mRNA_cap_ATPase/GuylTrfase_vir.
IPR004971. Pox_MCEL.
[Graphical view]

Pfam

PF10640. Pox_ATPase-GT. 1 hit.
PF03291. Pox_MCEL. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

MCEL_VACCW

Accession

Primary (citable) accession number: P04298
Secondary accession number(s): Q76ZS6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 1987
Last sequence update:	March 20, 1987
Last modified:	December 15, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)