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P04296

- DNBI_HHV11

UniProt

P04296 - DNBI_HHV11

Protein

Major DNA-binding protein

Gene

DBP

Organism
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (20 Mar 1987)
      Previous versions | rss
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    Functioni

    Plays several crucial roles in viral infection. Participates in the opening of the viral DNA origin to initiate replication by interacting with the origin-binding protein UL9. May disrupt loops, hairpins and other secondary structures present on ssDNA to reduce and eliminate pausing of viral DNA polymerase at specific sites during elongation. Promotes viral DNA recombination by performing strand-transfer, characterized by the ability to transfer a DNA strand from a linear duplex to a complementary single-stranded DNA circle. Can also catalyze the renaturation of complementary single strands.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri499 – 51214Sequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. single-stranded DNA binding Source: InterPro

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Major DNA-binding protein
    Alternative name(s):
    Infected cell protein 8
    Short name:
    ICP-8 protein
    Gene namesi
    Name:DBP
    Synonyms:ICP8
    ORF Names:UL29
    OrganismiHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
    Taxonomic identifieri10299 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000009294: Genome

    Subcellular locationi

    Host nucleus 1 Publication
    Note: In the absence of DNA replication, found in the nuclear framework-associated structures (prereplicative sites). As viral DNA replication proceeds, it migrates to globular intranuclear structures (replication compartments).

    GO - Cellular componenti

    1. host cell nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11961196Major DNA-binding proteinPRO_0000115743Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with UL9. Interacts with ICP27; this interaction plays a role in the stimulation of late gene transcription. Interacts with the alkaline exonuclease UL12; this interaction increases its nuclease processivity.3 Publications

    Protein-protein interaction databases

    BioGridi971471. 17 interactions.
    IntActiP04296. 53 interactions.
    MINTiMINT-191337.

    Structurei

    Secondary structure

    1
    1196
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi18 – 258
    Helixi31 – 377
    Beta strandi47 – 504
    Beta strandi64 – 7310
    Beta strandi81 – 9010
    Beta strandi92 – 987
    Helixi100 – 1023
    Helixi112 – 12312
    Helixi132 – 1376
    Helixi141 – 1488
    Beta strandi154 – 1629
    Turni163 – 1653
    Helixi166 – 1705
    Beta strandi174 – 1763
    Helixi178 – 1803
    Beta strandi182 – 1865
    Beta strandi189 – 19810
    Helixi199 – 2024
    Helixi234 – 24310
    Helixi245 – 2517
    Helixi257 – 26913
    Helixi310 – 33122
    Helixi342 – 3443
    Helixi346 – 3483
    Beta strandi353 – 3553
    Helixi358 – 37720
    Turni382 – 3843
    Beta strandi385 – 3906
    Beta strandi406 – 4116
    Turni413 – 4175
    Turni430 – 4334
    Helixi448 – 4547
    Turni455 – 4573
    Helixi459 – 46911
    Helixi485 – 4906
    Turni491 – 4944
    Turni504 – 5096
    Helixi511 – 5177
    Helixi519 – 5213
    Beta strandi533 – 5386
    Helixi575 – 58814
    Helixi603 – 6064
    Helixi610 – 63627
    Helixi643 – 6475
    Beta strandi651 – 6577
    Helixi667 – 69327
    Helixi703 – 71614
    Turni717 – 7204
    Beta strandi721 – 73111
    Turni744 – 7463
    Beta strandi748 – 7503
    Beta strandi759 – 7613
    Beta strandi764 – 77714
    Helixi810 – 8123
    Helixi816 – 8216
    Helixi823 – 8264
    Helixi840 – 84910
    Helixi861 – 87818
    Helixi888 – 90316
    Beta strandi913 – 92311
    Beta strandi925 – 9273
    Helixi928 – 9336
    Helixi941 – 95010
    Helixi951 – 9544
    Beta strandi960 – 9678
    Helixi969 – 9746
    Beta strandi978 – 98710
    Beta strandi999 – 10046
    Helixi1009 – 10113
    Beta strandi1022 – 10287
    Turni1031 – 10333
    Helixi1049 – 10568
    Helixi1064 – 107310
    Helixi1078 – 10825
    Helixi1085 – 10917
    Helixi1095 – 111117
    Helixi1120 – 11256

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1URJX-ray3.00A/B1-1136[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP04296.

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi929 – 9324Poly-Ala

    Sequence similaritiesi

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri499 – 51214Sequence AnalysisAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Family and domain databases

    InterProiIPR000635. Viral_ssDNA-bd.
    [Graphical view]
    PfamiPF00747. Viral_DNA_bp. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P04296-1 [UniParc]FASTAAdd to Basket

    « Hide

    METKPKTATT IKVPPGPLGY VYARACPSEG IELLALLSAR SGDSDVAVAP     50
    LVVGLTVESG FEANVAVVVG SRTTGLGGTA VSLKLTPSHY SSSVYVFHGG 100
    RHLDPSTQAP NLTRLCERAR RHFGFSDYTP RPGDLKHETT GEALCERLGL 150
    DPDRALLYLV VTEGFKEAVC INNTFLHLGG SDKVTIGGAE VHRIPVYPLQ 200
    LFMPDFSRVI AEPFNANHRS IGEKFTYPLP FFNRPLNRLL FEAVVGPAAV 250
    ALRCRNVDAV ARAAAHLAFD ENHEGAALPA DITFTAFEAS QGKTPRGGRD 300
    GGGKGAAGGF EQRLASVMAG DAALALESIV SMAVFDEPPT DISAWPLFEG 350
    QDTAAARANA VGAYLARAAG LVGAMVFSTN SALHLTEVDD AGPADPKDHS 400
    KPSFYRFFLV PGTHVAANPQ VDREGHVVPG FEGRPTAPLV GGTQEFAGEH 450
    LAMLCGFSPA LLAKMLFYLE RCDGAVIVGR QEMDVFRYVA DSNQTDVPCN 500
    LCTFDTRHAC VHTTLMRLRA RHPKFASAAR GAIGVFGTMN SMYSDCDVLG 550
    NYAAFSALKR ADGSETARTI MQETYRAATE RVMAELETLQ YVDQAVPTAM 600
    GRLETIITNR EALHTVVNNV RQVVDREVEQ LMRNLVEGRN FKFRDGLGEA 650
    NHAMSLTLDP YACGPCPLLQ LLGRRSNLAV YQDLALSQCH GVFAGQSVEG 700
    RNFRNQFQPV LRRRVMDMFN NGFLSAKTLT VALSEGAAIC APSLTAGQTA 750
    PAESSFEGDV ARVTLGFPKE LRVKSRVLFA GASANASEAA KARVASLQSA 800
    YQKPDKRVDI LLGPLGFLLK QFHAAIFPNG KPPGSNQPNP QWFWTALQRN 850
    QLPARLLSRE DIETIAFIKK FSLDYGAINF INLAPNNVSE LAMYYMANQI 900
    LRYCDHSTYF INTLTAIIAG SRRPPSVQAA AAWSAQGGAG LEAGARALMD 950
    AVDAHPGAWT SMFASCNLLR PVMAARPMVV LGLSISKYYG MAGNDRVFQA 1000
    GNWASLMGGK NACPLLIFDR TRKFVLACPR AGFVCAASSL GGGAHESSLC 1050
    EQLRGIISEG GAAVASSVFV ATVKSLGPRT QQLQIEDWLA LLEDEYLSEE 1100
    MMELTARALE RGNGEWSTDA ALEVAHEAEA LVSQLGNAGE VFNFGDFGCE 1150
    DDNATPFGGP GAPGPAFAGR KRAFHGDDPF GEGPPDKKGD LTLDML 1196
    Length:1,196
    Mass (Da):128,350
    Last modified:March 20, 1987 - v1
    Checksum:i453799162E5B99E9
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti44 – 441S → A in strain: Nonneuroinvasive mutant HF10.
    Natural varianti224 – 2241K → N in strain: Nonneuroinvasive mutant HF10 and 17 syn+.
    Natural varianti306 – 3061A → P in strain: Nonneuroinvasive mutant HF10 and 17 syn+.
    Natural varianti428 – 4281V → A in strain: Nonneuroinvasive mutant HF10.
    Natural varianti475 – 4751A → G in strain: Nonneuroinvasive mutant HF10 and 17 syn+.
    Natural varianti964 – 9641A → T in strain: Nonneuroinvasive mutant HF10.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14112 Genomic DNA. Translation: CAA32322.1.
    X03181 Genomic DNA. Translation: CAA26940.1.
    M21631 Genomic DNA. Translation: AAA45787.1.
    DQ889502 Genomic DNA. Translation: ABI63491.1.
    FJ593289 Genomic DNA. Translation: ACM62252.1.
    PIRiA03790. DNBEV1.
    RefSeqiNP_044631.1. NC_001806.1.

    Genome annotation databases

    GeneIDi2703458.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14112 Genomic DNA. Translation: CAA32322.1 .
    X03181 Genomic DNA. Translation: CAA26940.1 .
    M21631 Genomic DNA. Translation: AAA45787.1 .
    DQ889502 Genomic DNA. Translation: ABI63491.1 .
    FJ593289 Genomic DNA. Translation: ACM62252.1 .
    PIRi A03790. DNBEV1.
    RefSeqi NP_044631.1. NC_001806.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1URJ X-ray 3.00 A/B 1-1136 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 971471. 17 interactions.
    IntActi P04296. 53 interactions.
    MINTi MINT-191337.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 2703458.

    Miscellaneous databases

    EvolutionaryTracei P04296.

    Family and domain databases

    InterProi IPR000635. Viral_ssDNA-bd.
    [Graphical view ]
    Pfami PF00747. Viral_DNA_bp. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "DNA sequence of the region in the genome of herpes simplex virus type 1 containing the genes for DNA polymerase and the major DNA binding protein."
      Quinn J.P., McGeoch D.J.
      Nucleic Acids Res. 13:8143-8163(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1."
      McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.
      J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "Common epitopes of glycoprotein B map within the major DNA-binding proteins of bovine herpesvirus type 2 (BHV-2) and herpes simplex virus type 1 (HSV-1)."
      Hammerschmidt W., Conraths F., Mankertz J., Buhk H.-J., Pauli G., Ludwig H.
      Virology 165:406-418(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1062-1196.
    4. "Determination and analysis of the DNA sequence of highly attenuated herpes simplex virus type 1 mutant HF10, a potential oncolytic virus."
      Ushijima Y., Luo C., Goshima F., Yamauchi Y., Kimura H., Nishiyama Y.
      Microbes Infect. 9:142-149(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Nonneuroinvasive mutant HF10.
    5. "Herpes simplex virus type 1 bacterial artificial chromosome."
      Cunningham C., Davison A.J.
      Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 17 syn+.
    6. "Association of origin binding protein and single strand DNA-binding protein, ICP8, during herpes simplex virus type 1 DNA replication in vivo."
      Boehmer P.E., Craigie M.C., Stow N.D., Lehman I.R.
      J. Biol. Chem. 269:29329-29334(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH UL9, FUNCTION.
    7. "Herpes simplex virus type 1 prereplicative sites are a heterogeneous population: only a subset are likely to be precursors to replication compartments."
      Lukonis C.J., Burkham J., Weller S.K.
      J. Virol. 71:4771-4781(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    8. "The UL12.5 gene product of herpes simplex virus type 1 exhibits nuclease and strand exchange activities but does not localize to the nucleus."
      Reuven N.B., Antoku S., Weller S.K.
      J. Virol. 78:4599-4608(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH UL12, FUNCTION.
    9. "Evidence for a direct interaction between HSV-1 ICP27 and ICP8 proteins."
      Olesky M., McNamee E.E., Zhou C., Taylor T.J., Knipe D.M.
      Virology 331:94-105(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ICP27.
    10. "The crystal structure of the herpes simplex virus 1 ssDNA-binding protein suggests the structural basis for flexible, cooperative single-stranded DNA binding."
      Mapelli M., Panjikar S., Tucker P.A.
      J. Biol. Chem. 280:2990-2997(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 1-1136.

    Entry informationi

    Entry nameiDNBI_HHV11
    AccessioniPrimary (citable) accession number: P04296
    Secondary accession number(s): B9VQF7, Q09IA4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 20, 1987
    Last sequence update: March 20, 1987
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3