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P04296 (DNBI_HHV11) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Major DNA-binding protein
Alternative name(s):
Infected cell protein 8
Short name=ICP-8 protein
Gene names
Name:DBP
Synonyms:ICP8
ORF Names:UL29
OrganismHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) [Reference proteome]
Taxonomic identifier10299 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length1196 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays several crucial roles in viral infection. Participates in the opening of the viral DNA origin to initiate replication by interacting with the origin-binding protein UL9. May disrupt loops, hairpins and other secondary structures present on ssDNA to reduce and eliminate pausing of viral DNA polymerase at specific sites during elongation. Promotes viral DNA recombination by performing strand-transfer, characterized by the ability to transfer a DNA strand from a linear duplex to a complementary single-stranded DNA circle. Can also catalyze the renaturation of complementary single strands. Ref.6 Ref.8

Subunit structure

Interacts with UL9. Interacts with ICP27; this interaction plays a role in the stimulation of late gene transcription. Interacts with the alkaline exonuclease UL12; this interaction increases its nuclease processivity. Ref.6 Ref.8 Ref.9

Subcellular location

Host nucleus. Note: In the absence of DNA replication, found in the nuclear framework-associated structures (prereplicative sites). As viral DNA replication proceeds, it migrates to globular intranuclear structures (replication compartments). Ref.7

Sequence similarities

Belongs to the herpesviridae DNA-binding protein family.

Ontologies

Keywords
   Biological processDNA replication
   Cellular componentHost nucleus
   DomainZinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componenthost cell nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

single-stranded DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11961196Major DNA-binding protein
PRO_0000115743

Regions

Zinc finger499 – 51214 Potential
Compositional bias929 – 9324Poly-Ala

Natural variations

Natural variant441S → A in strain: Nonneuroinvasive mutant HF10.
Natural variant2241K → N in strain: Nonneuroinvasive mutant HF10 and 17 syn+.
Natural variant3061A → P in strain: Nonneuroinvasive mutant HF10 and 17 syn+.
Natural variant4281V → A in strain: Nonneuroinvasive mutant HF10.
Natural variant4751A → G in strain: Nonneuroinvasive mutant HF10 and 17 syn+.
Natural variant9641A → T in strain: Nonneuroinvasive mutant HF10.

Secondary structure

................................................................................................................................................. 1196
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P04296 [UniParc].

Last modified March 20, 1987. Version 1.
Checksum: 453799162E5B99E9

FASTA1,196128,350
        10         20         30         40         50         60 
METKPKTATT IKVPPGPLGY VYARACPSEG IELLALLSAR SGDSDVAVAP LVVGLTVESG 

        70         80         90        100        110        120 
FEANVAVVVG SRTTGLGGTA VSLKLTPSHY SSSVYVFHGG RHLDPSTQAP NLTRLCERAR 

       130        140        150        160        170        180 
RHFGFSDYTP RPGDLKHETT GEALCERLGL DPDRALLYLV VTEGFKEAVC INNTFLHLGG 

       190        200        210        220        230        240 
SDKVTIGGAE VHRIPVYPLQ LFMPDFSRVI AEPFNANHRS IGEKFTYPLP FFNRPLNRLL 

       250        260        270        280        290        300 
FEAVVGPAAV ALRCRNVDAV ARAAAHLAFD ENHEGAALPA DITFTAFEAS QGKTPRGGRD 

       310        320        330        340        350        360 
GGGKGAAGGF EQRLASVMAG DAALALESIV SMAVFDEPPT DISAWPLFEG QDTAAARANA 

       370        380        390        400        410        420 
VGAYLARAAG LVGAMVFSTN SALHLTEVDD AGPADPKDHS KPSFYRFFLV PGTHVAANPQ 

       430        440        450        460        470        480 
VDREGHVVPG FEGRPTAPLV GGTQEFAGEH LAMLCGFSPA LLAKMLFYLE RCDGAVIVGR 

       490        500        510        520        530        540 
QEMDVFRYVA DSNQTDVPCN LCTFDTRHAC VHTTLMRLRA RHPKFASAAR GAIGVFGTMN 

       550        560        570        580        590        600 
SMYSDCDVLG NYAAFSALKR ADGSETARTI MQETYRAATE RVMAELETLQ YVDQAVPTAM 

       610        620        630        640        650        660 
GRLETIITNR EALHTVVNNV RQVVDREVEQ LMRNLVEGRN FKFRDGLGEA NHAMSLTLDP 

       670        680        690        700        710        720 
YACGPCPLLQ LLGRRSNLAV YQDLALSQCH GVFAGQSVEG RNFRNQFQPV LRRRVMDMFN 

       730        740        750        760        770        780 
NGFLSAKTLT VALSEGAAIC APSLTAGQTA PAESSFEGDV ARVTLGFPKE LRVKSRVLFA 

       790        800        810        820        830        840 
GASANASEAA KARVASLQSA YQKPDKRVDI LLGPLGFLLK QFHAAIFPNG KPPGSNQPNP 

       850        860        870        880        890        900 
QWFWTALQRN QLPARLLSRE DIETIAFIKK FSLDYGAINF INLAPNNVSE LAMYYMANQI 

       910        920        930        940        950        960 
LRYCDHSTYF INTLTAIIAG SRRPPSVQAA AAWSAQGGAG LEAGARALMD AVDAHPGAWT 

       970        980        990       1000       1010       1020 
SMFASCNLLR PVMAARPMVV LGLSISKYYG MAGNDRVFQA GNWASLMGGK NACPLLIFDR 

      1030       1040       1050       1060       1070       1080 
TRKFVLACPR AGFVCAASSL GGGAHESSLC EQLRGIISEG GAAVASSVFV ATVKSLGPRT 

      1090       1100       1110       1120       1130       1140 
QQLQIEDWLA LLEDEYLSEE MMELTARALE RGNGEWSTDA ALEVAHEAEA LVSQLGNAGE 

      1150       1160       1170       1180       1190 
VFNFGDFGCE DDNATPFGGP GAPGPAFAGR KRAFHGDDPF GEGPPDKKGD LTLDML 

« Hide

References

« Hide 'large scale' references
[1]"DNA sequence of the region in the genome of herpes simplex virus type 1 containing the genes for DNA polymerase and the major DNA binding protein."
Quinn J.P., McGeoch D.J.
Nucleic Acids Res. 13:8143-8163(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1."
McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.
J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Common epitopes of glycoprotein B map within the major DNA-binding proteins of bovine herpesvirus type 2 (BHV-2) and herpes simplex virus type 1 (HSV-1)."
Hammerschmidt W., Conraths F., Mankertz J., Buhk H.-J., Pauli G., Ludwig H.
Virology 165:406-418(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1062-1196.
[4]"Determination and analysis of the DNA sequence of highly attenuated herpes simplex virus type 1 mutant HF10, a potential oncolytic virus."
Ushijima Y., Luo C., Goshima F., Yamauchi Y., Kimura H., Nishiyama Y.
Microbes Infect. 9:142-149(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Nonneuroinvasive mutant HF10.
[5]"Herpes simplex virus type 1 bacterial artificial chromosome."
Cunningham C., Davison A.J.
Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 17 syn+.
[6]"Association of origin binding protein and single strand DNA-binding protein, ICP8, during herpes simplex virus type 1 DNA replication in vivo."
Boehmer P.E., Craigie M.C., Stow N.D., Lehman I.R.
J. Biol. Chem. 269:29329-29334(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH UL9, FUNCTION.
[7]"Herpes simplex virus type 1 prereplicative sites are a heterogeneous population: only a subset are likely to be precursors to replication compartments."
Lukonis C.J., Burkham J., Weller S.K.
J. Virol. 71:4771-4781(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[8]"The UL12.5 gene product of herpes simplex virus type 1 exhibits nuclease and strand exchange activities but does not localize to the nucleus."
Reuven N.B., Antoku S., Weller S.K.
J. Virol. 78:4599-4608(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH UL12, FUNCTION.
[9]"Evidence for a direct interaction between HSV-1 ICP27 and ICP8 proteins."
Olesky M., McNamee E.E., Zhou C., Taylor T.J., Knipe D.M.
Virology 331:94-105(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ICP27.
[10]"The crystal structure of the herpes simplex virus 1 ssDNA-binding protein suggests the structural basis for flexible, cooperative single-stranded DNA binding."
Mapelli M., Panjikar S., Tucker P.A.
J. Biol. Chem. 280:2990-2997(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 1-1136.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X14112 Genomic DNA. Translation: CAA32322.1.
X03181 Genomic DNA. Translation: CAA26940.1.
M21631 Genomic DNA. Translation: AAA45787.1.
DQ889502 Genomic DNA. Translation: ABI63491.1.
FJ593289 Genomic DNA. Translation: ACM62252.1.
PIRDNBEV1. A03790.
RefSeqNP_044631.1. NC_001806.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1URJX-ray3.00A/B1-1136[»]
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid971471. 17 interactions.
IntActP04296. 54 interactions.
MINTMINT-191337.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2703458.

Family and domain databases

InterProIPR000635. Viral_ssDNA-bd.
[Graphical view]
PfamPF00747. Viral_DNA_bp. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP04296.

Entry information

Entry nameDNBI_HHV11
AccessionPrimary (citable) accession number: P04296
Secondary accession number(s): B9VQF7, Q09IA4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: March 20, 1987
Last modified: May 14, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references