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P04296

- DNBI_HHV11

UniProt

P04296 - DNBI_HHV11

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Protein

Major DNA-binding protein

Gene
DBP, ICP8, UL29
Organism
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Plays several crucial roles in viral infection. Participates in the opening of the viral DNA origin to initiate replication by interacting with the origin-binding protein UL9. May disrupt loops, hairpins and other secondary structures present on ssDNA to reduce and eliminate pausing of viral DNA polymerase at specific sites during elongation. Promotes viral DNA recombination by performing strand-transfer, characterized by the ability to transfer a DNA strand from a linear duplex to a complementary single-stranded DNA circle. Can also catalyze the renaturation of complementary single strands.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri499 – 51214 Reviewed predictionAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. single-stranded DNA binding Source: InterPro

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Major DNA-binding protein
Alternative name(s):
Infected cell protein 8
Short name:
ICP-8 protein
Gene namesi
Name:DBP
Synonyms:ICP8
ORF Names:UL29
OrganismiHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Taxonomic identifieri10299 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000009294: Genome

Subcellular locationi

Host nucleus
Note: In the absence of DNA replication, found in the nuclear framework-associated structures (prereplicative sites). As viral DNA replication proceeds, it migrates to globular intranuclear structures (replication compartments).1 Publication

GO - Cellular componenti

  1. host cell nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11961196Major DNA-binding proteinPRO_0000115743Add
BLAST

Interactioni

Subunit structurei

Interacts with UL9. Interacts with ICP27; this interaction plays a role in the stimulation of late gene transcription. Interacts with the alkaline exonuclease UL12; this interaction increases its nuclease processivity.3 Publications

Protein-protein interaction databases

BioGridi971471. 17 interactions.
IntActiP04296. 54 interactions.
MINTiMINT-191337.

Structurei

Secondary structure

1
1196
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi18 – 258
Helixi31 – 377
Beta strandi47 – 504
Beta strandi64 – 7310
Beta strandi81 – 9010
Beta strandi92 – 987
Helixi100 – 1023
Helixi112 – 12312
Helixi132 – 1376
Helixi141 – 1488
Beta strandi154 – 1629
Turni163 – 1653
Helixi166 – 1705
Beta strandi174 – 1763
Helixi178 – 1803
Beta strandi182 – 1865
Beta strandi189 – 19810
Helixi199 – 2024
Helixi234 – 24310
Helixi245 – 2517
Helixi257 – 26913
Helixi310 – 33122
Helixi342 – 3443
Helixi346 – 3483
Beta strandi353 – 3553
Helixi358 – 37720
Turni382 – 3843
Beta strandi385 – 3906
Beta strandi406 – 4116
Turni413 – 4175
Turni430 – 4334
Helixi448 – 4547
Turni455 – 4573
Helixi459 – 46911
Helixi485 – 4906
Turni491 – 4944
Turni504 – 5096
Helixi511 – 5177
Helixi519 – 5213
Beta strandi533 – 5386
Helixi575 – 58814
Helixi603 – 6064
Helixi610 – 63627
Helixi643 – 6475
Beta strandi651 – 6577
Helixi667 – 69327
Helixi703 – 71614
Turni717 – 7204
Beta strandi721 – 73111
Turni744 – 7463
Beta strandi748 – 7503
Beta strandi759 – 7613
Beta strandi764 – 77714
Helixi810 – 8123
Helixi816 – 8216
Helixi823 – 8264
Helixi840 – 84910
Helixi861 – 87818
Helixi888 – 90316
Beta strandi913 – 92311
Beta strandi925 – 9273
Helixi928 – 9336
Helixi941 – 95010
Helixi951 – 9544
Beta strandi960 – 9678
Helixi969 – 9746
Beta strandi978 – 98710
Beta strandi999 – 10046
Helixi1009 – 10113
Beta strandi1022 – 10287
Turni1031 – 10333
Helixi1049 – 10568
Helixi1064 – 107310
Helixi1078 – 10825
Helixi1085 – 10917
Helixi1095 – 111117
Helixi1120 – 11256

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1URJX-ray3.00A/B1-1136[»]

Miscellaneous databases

EvolutionaryTraceiP04296.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi929 – 9324Poly-Ala

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri499 – 51214 Reviewed predictionAdd
BLAST

Keywords - Domaini

Zinc-finger

Family and domain databases

InterProiIPR000635. Viral_ssDNA-bd.
[Graphical view]
PfamiPF00747. Viral_DNA_bp. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04296-1 [UniParc]FASTAAdd to Basket

« Hide

METKPKTATT IKVPPGPLGY VYARACPSEG IELLALLSAR SGDSDVAVAP     50
LVVGLTVESG FEANVAVVVG SRTTGLGGTA VSLKLTPSHY SSSVYVFHGG 100
RHLDPSTQAP NLTRLCERAR RHFGFSDYTP RPGDLKHETT GEALCERLGL 150
DPDRALLYLV VTEGFKEAVC INNTFLHLGG SDKVTIGGAE VHRIPVYPLQ 200
LFMPDFSRVI AEPFNANHRS IGEKFTYPLP FFNRPLNRLL FEAVVGPAAV 250
ALRCRNVDAV ARAAAHLAFD ENHEGAALPA DITFTAFEAS QGKTPRGGRD 300
GGGKGAAGGF EQRLASVMAG DAALALESIV SMAVFDEPPT DISAWPLFEG 350
QDTAAARANA VGAYLARAAG LVGAMVFSTN SALHLTEVDD AGPADPKDHS 400
KPSFYRFFLV PGTHVAANPQ VDREGHVVPG FEGRPTAPLV GGTQEFAGEH 450
LAMLCGFSPA LLAKMLFYLE RCDGAVIVGR QEMDVFRYVA DSNQTDVPCN 500
LCTFDTRHAC VHTTLMRLRA RHPKFASAAR GAIGVFGTMN SMYSDCDVLG 550
NYAAFSALKR ADGSETARTI MQETYRAATE RVMAELETLQ YVDQAVPTAM 600
GRLETIITNR EALHTVVNNV RQVVDREVEQ LMRNLVEGRN FKFRDGLGEA 650
NHAMSLTLDP YACGPCPLLQ LLGRRSNLAV YQDLALSQCH GVFAGQSVEG 700
RNFRNQFQPV LRRRVMDMFN NGFLSAKTLT VALSEGAAIC APSLTAGQTA 750
PAESSFEGDV ARVTLGFPKE LRVKSRVLFA GASANASEAA KARVASLQSA 800
YQKPDKRVDI LLGPLGFLLK QFHAAIFPNG KPPGSNQPNP QWFWTALQRN 850
QLPARLLSRE DIETIAFIKK FSLDYGAINF INLAPNNVSE LAMYYMANQI 900
LRYCDHSTYF INTLTAIIAG SRRPPSVQAA AAWSAQGGAG LEAGARALMD 950
AVDAHPGAWT SMFASCNLLR PVMAARPMVV LGLSISKYYG MAGNDRVFQA 1000
GNWASLMGGK NACPLLIFDR TRKFVLACPR AGFVCAASSL GGGAHESSLC 1050
EQLRGIISEG GAAVASSVFV ATVKSLGPRT QQLQIEDWLA LLEDEYLSEE 1100
MMELTARALE RGNGEWSTDA ALEVAHEAEA LVSQLGNAGE VFNFGDFGCE 1150
DDNATPFGGP GAPGPAFAGR KRAFHGDDPF GEGPPDKKGD LTLDML 1196
Length:1,196
Mass (Da):128,350
Last modified:March 20, 1987 - v1
Checksum:i453799162E5B99E9
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti44 – 441S → A in strain: Nonneuroinvasive mutant HF10.
Natural varianti224 – 2241K → N in strain: Nonneuroinvasive mutant HF10 and 17 syn+.
Natural varianti306 – 3061A → P in strain: Nonneuroinvasive mutant HF10 and 17 syn+.
Natural varianti428 – 4281V → A in strain: Nonneuroinvasive mutant HF10.
Natural varianti475 – 4751A → G in strain: Nonneuroinvasive mutant HF10 and 17 syn+.
Natural varianti964 – 9641A → T in strain: Nonneuroinvasive mutant HF10.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X14112 Genomic DNA. Translation: CAA32322.1.
X03181 Genomic DNA. Translation: CAA26940.1.
M21631 Genomic DNA. Translation: AAA45787.1.
DQ889502 Genomic DNA. Translation: ABI63491.1.
FJ593289 Genomic DNA. Translation: ACM62252.1.
PIRiA03790. DNBEV1.
RefSeqiNP_044631.1. NC_001806.1.

Genome annotation databases

GeneIDi2703458.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X14112 Genomic DNA. Translation: CAA32322.1 .
X03181 Genomic DNA. Translation: CAA26940.1 .
M21631 Genomic DNA. Translation: AAA45787.1 .
DQ889502 Genomic DNA. Translation: ABI63491.1 .
FJ593289 Genomic DNA. Translation: ACM62252.1 .
PIRi A03790. DNBEV1.
RefSeqi NP_044631.1. NC_001806.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1URJ X-ray 3.00 A/B 1-1136 [» ]
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 971471. 17 interactions.
IntActi P04296. 54 interactions.
MINTi MINT-191337.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2703458.

Miscellaneous databases

EvolutionaryTracei P04296.

Family and domain databases

InterProi IPR000635. Viral_ssDNA-bd.
[Graphical view ]
Pfami PF00747. Viral_DNA_bp. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "DNA sequence of the region in the genome of herpes simplex virus type 1 containing the genes for DNA polymerase and the major DNA binding protein."
    Quinn J.P., McGeoch D.J.
    Nucleic Acids Res. 13:8143-8163(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1."
    McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.
    J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Common epitopes of glycoprotein B map within the major DNA-binding proteins of bovine herpesvirus type 2 (BHV-2) and herpes simplex virus type 1 (HSV-1)."
    Hammerschmidt W., Conraths F., Mankertz J., Buhk H.-J., Pauli G., Ludwig H.
    Virology 165:406-418(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1062-1196.
  4. "Determination and analysis of the DNA sequence of highly attenuated herpes simplex virus type 1 mutant HF10, a potential oncolytic virus."
    Ushijima Y., Luo C., Goshima F., Yamauchi Y., Kimura H., Nishiyama Y.
    Microbes Infect. 9:142-149(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Nonneuroinvasive mutant HF10.
  5. "Herpes simplex virus type 1 bacterial artificial chromosome."
    Cunningham C., Davison A.J.
    Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 17 syn+.
  6. "Association of origin binding protein and single strand DNA-binding protein, ICP8, during herpes simplex virus type 1 DNA replication in vivo."
    Boehmer P.E., Craigie M.C., Stow N.D., Lehman I.R.
    J. Biol. Chem. 269:29329-29334(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UL9, FUNCTION.
  7. "Herpes simplex virus type 1 prereplicative sites are a heterogeneous population: only a subset are likely to be precursors to replication compartments."
    Lukonis C.J., Burkham J., Weller S.K.
    J. Virol. 71:4771-4781(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  8. "The UL12.5 gene product of herpes simplex virus type 1 exhibits nuclease and strand exchange activities but does not localize to the nucleus."
    Reuven N.B., Antoku S., Weller S.K.
    J. Virol. 78:4599-4608(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UL12, FUNCTION.
  9. "Evidence for a direct interaction between HSV-1 ICP27 and ICP8 proteins."
    Olesky M., McNamee E.E., Zhou C., Taylor T.J., Knipe D.M.
    Virology 331:94-105(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ICP27.
  10. "The crystal structure of the herpes simplex virus 1 ssDNA-binding protein suggests the structural basis for flexible, cooperative single-stranded DNA binding."
    Mapelli M., Panjikar S., Tucker P.A.
    J. Biol. Chem. 280:2990-2997(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 1-1136.

Entry informationi

Entry nameiDNBI_HHV11
AccessioniPrimary (citable) accession number: P04296
Secondary accession number(s): B9VQF7, Q09IA4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: March 20, 1987
Last modified: May 14, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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