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Protein

Tripartite terminase subunit 3

Gene

TRM3

Organism
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the molecular motor that translocates viral genomic DNA in empty capsid during DNA packaging. Forms a tripartite terminase complex together with TRM1 and TRM2 in the host cytoplasm. Once the complex reaches the host nucleus, it interacts with the capsid portal vertex. This portal forms a ring in which genomic DNA is translocated into the capsid. TRM3 carries an RNase H-like nuclease activity that plays an important role for the cleavage of concatemeric viral DNA into unit length genomes.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei357 – 3571For ATPase activityUniRule annotation
Active sitei509 – 5091For nuclease activityUniRule annotation1 Publication
Active sitei581 – 5811For nuclease activityUniRule annotation1 Publication
Active sitei707 – 7071For nuclease activityUniRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Viral genome packaging, Virus exit from host cell

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tripartite terminase subunit 3UniRule annotation (EC:3.1.-.-UniRule annotation)
Alternative name(s):
Terminase large subunitUniRule annotation
Gene namesi
Name:TRM3UniRule annotation
Ordered Locus Names:UL15
OrganismiHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Taxonomic identifieri10299 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000009294 Componenti: Genome

Subcellular locationi

  • Host nucleus UniRule annotation2 Publications

  • Note: Responsible for the nuclear localization of the two others subunits TRM1 and TRM2.UniRule annotation1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 735735Tripartite terminase subunit 3PRO_0000115940Add
BLAST

Interactioni

Subunit structurei

Interacts with the terminase subunits TRM1 and TRM2. Interacts with portal protein.UniRule annotation3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
UL28P102128EBI-7032965,EBI-7032948
UL33P102176EBI-7032965,EBI-7033000

Protein-protein interaction databases

IntActiP04295. 3 interactions.
MINTiMINT-6732654.

Structurei

Secondary structure

1
735
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi480 – 4889Combined sources
Helixi497 – 4993Combined sources
Beta strandi502 – 5098Combined sources
Beta strandi514 – 5163Combined sources
Beta strandi520 – 52910Combined sources
Beta strandi532 – 54211Combined sources
Helixi550 – 56819Combined sources
Beta strandi574 – 5818Combined sources
Helixi586 – 60015Combined sources
Beta strandi616 – 6194Combined sources
Helixi640 – 65213Combined sources
Beta strandi656 – 6638Combined sources
Beta strandi665 – 6717Combined sources
Helixi673 – 6819Combined sources
Helixi707 – 71913Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4IOXX-ray2.46A/B/C471-735[»]
5HUWX-ray1.95A/B181-192[»]
ProteinModelPortaliP04295.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi183 – 1897Nuclear localization signalUniRule annotation1 Publication
Motifi258 – 2658Walker A motifUniRule annotation
Motifi352 – 3576Walker B motifUniRule annotation

Sequence similaritiesi

Belongs to the herpesviridae TRM3 protein family.UniRule annotation

Family and domain databases

HAMAPiMF_04013. HSV_TRM3.
InterProiIPR003498. DNA_pack_C.
IPR003499. DNA_pack_N.
[Graphical view]
PfamiPF02499. DNA_pack_C. 1 hit.
PF02500. DNA_pack_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04295-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFGQQLASDV QQYLERLEKQ RQLKVGADEA SAGLTMGGDA LRVPFLDFAT
60 70 80 90 100
ATPKRHQTVV PGVGTLHDCC EHSPLFSAVA RRLLFNSLVP AQLKGRDFGG
110 120 130 140 150
DHTAKLEFLA PELVRAVARL RFKECAPADV VPQRNAYYSV LNTFQALHRS
160 170 180 190 200
EAFRQLVHFV RDFAQLLKTS FRASSLTETT GPPKKRAKVD VATHGRTYGT
210 220 230 240 250
LELFQKMILM HATYFLAAVL LGDHAEQVNT FLRLVFEIPL FSDAAVRHFR
260 270 280 290 300
QRATVFLVPR RHGKTWFLVP LIALSLASFR GIKIGYTAHI RKATEPVFEE
310 320 330 340 350
IDACLRGWFG SARVDHVKGE TISFSFPDGS RSTIVFASSH NTNGIRGQDF
360 370 380 390 400
NLLFVDEANF IRPDAVQTIM GFLNQANCKI IFVSSTNTGK ASTSFLYNLR
410 420 430 440 450
GAADELLNVV TYICDDHMPR VVTHTNATAC SCYILNKPVF ITMDGAVRRT
460 470 480 490 500
ADLFLADSFM QEIIGGQARE TGDDRPVLTK SAGERFLLYR PSTTTNSGLM
510 520 530 540 550
APDLYVYVDP AFTANTRASG TGVAVVGRYR DDYIIFALEH FFLRALTGSA
560 570 580 590 600
PADIARCVVH SLTQVLALHP GAFRGVRVAV EGNSSQDSAV AIATHVHTEM
610 620 630 640 650
HRLLASEGAD AGSGPELLFY HCEPPGSAVL YPFFLLNKQK TPAFEHFIKK
660 670 680 690 700
FNSGGVMASQ EIVSATVRLQ TDPVEYLLEQ LNNLTETVSP NTDVRTYSGK
710 720 730
RNGASDDLMV AVIMAIYLAA QAGPPHTFAP ITRVS
Length:735
Mass (Da):80,923
Last modified:July 1, 1989 - v2
Checksum:iA7B8D69C3E6CD965
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14112 Genomic DNA. Translation: CAA32330.1.
X03839 Genomic DNA. Translation: CAA27456.1. Different termination.
PIRiA03781. WMBE31.
F30083. WMBET5.
RefSeqiYP_009137089.1. NC_001806.2.

Genome annotation databases

GeneIDi2703385.
KEGGivg:2703385.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14112 Genomic DNA. Translation: CAA32330.1.
X03839 Genomic DNA. Translation: CAA27456.1. Different termination.
PIRiA03781. WMBE31.
F30083. WMBET5.
RefSeqiYP_009137089.1. NC_001806.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4IOXX-ray2.46A/B/C471-735[»]
5HUWX-ray1.95A/B181-192[»]
ProteinModelPortaliP04295.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP04295. 3 interactions.
MINTiMINT-6732654.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2703385.
KEGGivg:2703385.

Family and domain databases

HAMAPiMF_04013. HSV_TRM3.
InterProiIPR003498. DNA_pack_C.
IPR003499. DNA_pack_N.
[Graphical view]
PfamiPF02499. DNA_pack_C. 1 hit.
PF02500. DNA_pack_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1."
    McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.
    J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "DNA sequence of the region in the genome of herpes simplex virus type 1 containing the exonuclease gene and neighbouring genes."
    McGeoch D.J., Dolan A., Frame M.C.
    Nucleic Acids Res. 14:3435-3448(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-343.
  3. "Herpes simplex virus type 1 portal protein UL6 interacts with the putative terminase subunits UL15 and UL28."
    White C.A., Stow N.D., Patel A.H., Hughes M., Preston V.G.
    J. Virol. 77:6351-6358(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PORTAL PROTEIN.
  4. "Herpes simplex virus 1 DNA packaging proteins encoded by UL6, UL15, UL17, UL28, and UL33 are located on the external surface of the viral capsid."
    Wills E., Scholtes L., Baines J.D.
    J. Virol. 80:10894-10899(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  5. "Putative terminase subunits of herpes simplex virus 1 form a complex in the cytoplasm and interact with portal protein in the nucleus."
    Yang K., Homa F., Baines J.D.
    J. Virol. 81:6419-6433(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TRM1 AND PORTAL PROTEIN, SUBCELLULAR LOCATION, NUCLEAR LOCALIZATION SIGNAL.
  6. "The bacteriophage DNA packaging motor."
    Rao V.B., Feiss M.
    Annu. Rev. Genet. 42:647-681(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  7. "The putative leucine zipper of the UL6-encoded portal protein of herpes simplex virus 1 is necessary for interaction with pUL15 and pUL28 and their association with capsids."
    Yang K., Wills E., Baines J.D.
    J. Virol. 83:4557-4564(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UL6.
  8. "The structure of the herpes simplex virus DNA-packaging terminase pUL15 nuclease domain suggests an evolutionary lineage among eukaryotic and prokaryotic viruses."
    Selvarajan Sigamani S., Zhao H., Kamau Y.N., Baines J.D., Tang L.
    J. Virol. 87:7140-7148(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.46 ANGSTROMS) OF 471-735.

Entry informationi

Entry nameiTRM3_HHV11
AccessioniPrimary (citable) accession number: P04295
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: July 1, 1989
Last modified: June 8, 2016
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.