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Protein

Tripartite terminase subunit 3

Gene

TRM3

Organism
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the molecular motor that translocates viral genomic DNA in empty capsid during DNA packaging. Forms a tripartite terminase complex together with TRM1 and TRM2 in the host cytoplasm. Once the complex reaches the host nucleus, it interacts with the capsid portal vertex. This portal forms a ring in which genomic DNA is translocated into the capsid. TRM3 carries an RNase H-like nuclease activity that plays an important role for the cleavage of concatemeric viral DNA into unit length genomes.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei357For ATPase activityUniRule annotation1
Active sitei509For nuclease activityUniRule annotation1 Publication1
Active sitei581For nuclease activityUniRule annotation1 Publication1
Active sitei707For nuclease activityUniRule annotation1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Hydrolase
Biological processViral genome packaging, Viral release from host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Tripartite terminase subunit 3UniRule annotation (EC:3.1.-.-UniRule annotation)
Alternative name(s):
Terminase large subunitUniRule annotation
Gene namesi
Name:TRM3UniRule annotation
Ordered Locus Names:UL15
OrganismiHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Taxonomic identifieri10299 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000009294 Componenti: Genome

Subcellular locationi

  • Host nucleus UniRule annotation2 Publications
  • Note: Responsible for the nuclear localization of the two others subunits TRM1 and TRM2.UniRule annotation1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001159401 – 735Tripartite terminase subunit 3Add BLAST735

Proteomic databases

PRIDEiP04295

Interactioni

Subunit structurei

Interacts with the terminase subunits TRM1 and TRM2. Interacts with portal protein.UniRule annotation3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

IntActiP04295, 3 interactors
MINTiP04295

Structurei

Secondary structure

1735
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi480 – 488Combined sources9
Helixi497 – 499Combined sources3
Beta strandi502 – 509Combined sources8
Beta strandi514 – 516Combined sources3
Beta strandi520 – 529Combined sources10
Beta strandi532 – 542Combined sources11
Helixi550 – 568Combined sources19
Beta strandi574 – 581Combined sources8
Helixi586 – 600Combined sources15
Beta strandi616 – 619Combined sources4
Helixi640 – 652Combined sources13
Beta strandi656 – 663Combined sources8
Beta strandi665 – 671Combined sources7
Helixi673 – 681Combined sources9
Helixi707 – 719Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4IOXX-ray2.46A/B/C471-735[»]
5HUWX-ray1.95A/B181-192[»]
ProteinModelPortaliP04295
SMRiP04295
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi183 – 189Nuclear localization signalUniRule annotation1 Publication7
Motifi258 – 265Walker A motifUniRule annotation8
Motifi352 – 357Walker B motifUniRule annotation6

Sequence similaritiesi

Belongs to the herpesviridae TRM3 protein family.UniRule annotation

Phylogenomic databases

OrthoDBiVOG0900002F

Family and domain databases

Gene3Di3.30.420.320, 1 hit
HAMAPiMF_04013 HSV_TRM3, 1 hit
InterProiView protein in InterPro
IPR003498 DNA_pack_C
IPR038435 DNA_pack_C_sf
IPR003499 DNA_pack_N
IPR033663 HSV_TRM3
PfamiView protein in Pfam
PF02499 DNA_pack_C, 1 hit
PF02500 DNA_pack_N, 1 hit

Sequencei

Sequence statusi: Complete.

P04295-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFGQQLASDV QQYLERLEKQ RQLKVGADEA SAGLTMGGDA LRVPFLDFAT
60 70 80 90 100
ATPKRHQTVV PGVGTLHDCC EHSPLFSAVA RRLLFNSLVP AQLKGRDFGG
110 120 130 140 150
DHTAKLEFLA PELVRAVARL RFKECAPADV VPQRNAYYSV LNTFQALHRS
160 170 180 190 200
EAFRQLVHFV RDFAQLLKTS FRASSLTETT GPPKKRAKVD VATHGRTYGT
210 220 230 240 250
LELFQKMILM HATYFLAAVL LGDHAEQVNT FLRLVFEIPL FSDAAVRHFR
260 270 280 290 300
QRATVFLVPR RHGKTWFLVP LIALSLASFR GIKIGYTAHI RKATEPVFEE
310 320 330 340 350
IDACLRGWFG SARVDHVKGE TISFSFPDGS RSTIVFASSH NTNGIRGQDF
360 370 380 390 400
NLLFVDEANF IRPDAVQTIM GFLNQANCKI IFVSSTNTGK ASTSFLYNLR
410 420 430 440 450
GAADELLNVV TYICDDHMPR VVTHTNATAC SCYILNKPVF ITMDGAVRRT
460 470 480 490 500
ADLFLADSFM QEIIGGQARE TGDDRPVLTK SAGERFLLYR PSTTTNSGLM
510 520 530 540 550
APDLYVYVDP AFTANTRASG TGVAVVGRYR DDYIIFALEH FFLRALTGSA
560 570 580 590 600
PADIARCVVH SLTQVLALHP GAFRGVRVAV EGNSSQDSAV AIATHVHTEM
610 620 630 640 650
HRLLASEGAD AGSGPELLFY HCEPPGSAVL YPFFLLNKQK TPAFEHFIKK
660 670 680 690 700
FNSGGVMASQ EIVSATVRLQ TDPVEYLLEQ LNNLTETVSP NTDVRTYSGK
710 720 730
RNGASDDLMV AVIMAIYLAA QAGPPHTFAP ITRVS
Length:735
Mass (Da):80,923
Last modified:July 1, 1989 - v2
Checksum:iA7B8D69C3E6CD965
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14112 Genomic DNA Translation: CAA32330.1
X03839 Genomic DNA Translation: CAA27456.1 Different termination.
PIRiA03781 WMBE31
F30083 WMBET5
RefSeqiYP_009137089.1, NC_001806.2

Genome annotation databases

GeneIDi2703385
KEGGivg:2703385

Similar proteinsi

Entry informationi

Entry nameiTRM3_HHV11
AccessioniPrimary (citable) accession number: P04295
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: July 1, 1989
Last modified: March 28, 2018
This is version 86 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health