P04295 (TRM3_HHV11) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tripartite terminase subunit UL15

Alternative name(s):
DNA-packaging protein UL15
Terminase large subunit

Gene names

Name:

UL15

Organism

Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) [Reference proteome]

Taxonomic identifier

10299 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Simplexvirus › Human herpesvirus 1

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	735 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the molecular motor that translocates genomic DNA in empty capsid during DNA packaging. Heterodimerizes with small terminase protein to be docked on capsid portal protein. The latter forms a ring in which genomic DNA in translocated into the capsid. May have or induce an endonuclease activity to cleave the genome concatemer after encapsidation.
Subunit structure	Interacts with UL28 and UL6. Ref.3 Ref.5 Ref.7
Subcellular location	Host nucleus Ref.4.
Sequence similarities	Belongs to the herpesviridae UL15 protein family.

Ontologies

Keywords
Biological process	Viral genome packaging
Cellular component	Host nucleus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA packaging Inferred from electronic annotation. Source: InterPro
Cellular_component	host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 735	735	Tripartite terminase subunit UL15		PRO_0000115940
Regions
	Motif	258 – 265	8	Walker A motif
	Motif	352 – 357	6	Walker B motif
Sites
	Active site	357	1	For ATPase activity Potential

Sequences

	Sequence		Length	Mass (Da)
	P04295 [UniParc]. Last modified July 1, 1989. Version 2. Checksum: A7B8D69C3E6CD965	FASTA	735	80,923
	10 20 30 40 50 60 MFGQQLASDV QQYLERLEKQ RQLKVGADEA SAGLTMGGDA LRVPFLDFAT ATPKRHQTVV 70 80 90 100 110 120 PGVGTLHDCC EHSPLFSAVA RRLLFNSLVP AQLKGRDFGG DHTAKLEFLA PELVRAVARL 130 140 150 160 170 180 RFKECAPADV VPQRNAYYSV LNTFQALHRS EAFRQLVHFV RDFAQLLKTS FRASSLTETT 190 200 210 220 230 240 GPPKKRAKVD VATHGRTYGT LELFQKMILM HATYFLAAVL LGDHAEQVNT FLRLVFEIPL 250 260 270 280 290 300 FSDAAVRHFR QRATVFLVPR RHGKTWFLVP LIALSLASFR GIKIGYTAHI RKATEPVFEE 310 320 330 340 350 360 IDACLRGWFG SARVDHVKGE TISFSFPDGS RSTIVFASSH NTNGIRGQDF NLLFVDEANF 370 380 390 400 410 420 IRPDAVQTIM GFLNQANCKI IFVSSTNTGK ASTSFLYNLR GAADELLNVV TYICDDHMPR 430 440 450 460 470 480 VVTHTNATAC SCYILNKPVF ITMDGAVRRT ADLFLADSFM QEIIGGQARE TGDDRPVLTK 490 500 510 520 530 540 SAGERFLLYR PSTTTNSGLM APDLYVYVDP AFTANTRASG TGVAVVGRYR DDYIIFALEH 550 560 570 580 590 600 FFLRALTGSA PADIARCVVH SLTQVLALHP GAFRGVRVAV EGNSSQDSAV AIATHVHTEM 610 620 630 640 650 660 HRLLASEGAD AGSGPELLFY HCEPPGSAVL YPFFLLNKQK TPAFEHFIKK FNSGGVMASQ 670 680 690 700 710 720 EIVSATVRLQ TDPVEYLLEQ LNNLTETVSP NTDVRTYSGK RNGASDDLMV AVIMAIYLAA 730 QAGPPHTFAP ITRVS

References

[1]	"The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1." McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P. J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"DNA sequence of the region in the genome of herpes simplex virus type 1 containing the exonuclease gene and neighbouring genes." McGeoch D.J., Dolan A., Frame M.C. Nucleic Acids Res. 14:3435-3448(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-343.
[3]	"Herpes simplex virus type 1 portal protein UL6 interacts with the putative terminase subunits UL15 and UL28." White C.A., Stow N.D., Patel A.H., Hughes M., Preston V.G. J. Virol. 77:6351-6358(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH UL6.
[4]	"Herpes simplex virus 1 DNA packaging proteins encoded by UL6, UL15, UL17, UL28, and UL33 are located on the external surface of the viral capsid." Wills E., Scholtes L., Baines J.D. J. Virol. 80:10894-10899(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[5]	"Putative terminase subunits of herpes simplex virus 1 form a complex in the cytoplasm and interact with portal protein in the nucleus." Yang K., Homa F., Baines J.D. J. Virol. 81:6419-6433(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH UL28 AND UL6.
[6]	"The bacteriophage DNA packaging motor." Rao V.B., Feiss M. Annu. Rev. Genet. 42:647-681(2008) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[7]	"The putative leucine zipper of the UL6-encoded portal protein of herpes simplex virus 1 is necessary for interaction with pUL15 and pUL28 and their association with capsids." Yang K., Wills E., Baines J.D. J. Virol. 83:4557-4564(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH UL6.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X14112 Genomic DNA. Translation: CAA32330.1. X03839 Genomic DNA. Translation: CAA27456.1. Different termination.
PIR	WMBE31. A03781. WMBET5. F30083.
RefSeq	NP_044616.1. NC_001806.1.
3D structure databases
ProteinModelPortal	P04295.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-6732654.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2703385.
Phylogenomic databases
ProtClustDB	PHA3368.
Family and domain databases
InterPro	IPR003498. DNA_pack_C. IPR003499. DNA_pack_N. [Graphical view]
Pfam	PF02499. DNA_pack_C. 1 hit. PF02500. DNA_pack_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRM3_HHV11

Accession

Primary (citable) accession number: P04295

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 1987
Last sequence update:	July 1, 1989
Last modified:	April 3, 2013
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program