P04288 (GM_HHV11) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Envelope glycoprotein M
Short name=gM

Gene names

Name:	gM
ORF Names:	UL10

Organism

Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) [Reference proteome]

Taxonomic identifier

10299 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Simplexvirus › Human herpesvirus 1

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Envelope glycoprotein important for virion assembly and egress. May be involved in capsid budding through the inner nuclear membrane during egress. Also seems to be important for cell-to-cell spread and/or virus entry By similarity.
Subunit structure	Interacts with gN By similarity. The gM-gN heterodimer forms the gCII complex By similarity.
Subcellular location	Virion membrane; Multi-pass membrane protein By similarity. Host Golgi apparatus membrane; Multi-pass membrane protein Probable. Host endosome membrane; Multi-pass membrane protein By similarity. Host nucleus inner membrane; Multi-pass membrane protein. Note: gM recruitment to the nucleus inner membrane requires UL31 and UL34. During virion morphogenesis, this protein probably accumulates in the endosomes and trans-Golgi where secondary envelopment occurs. Ref.6 Ref.7 Ref.8
Sequence similarities	Belongs to the herpesviridae glycoprotein M family.

Ontologies

Keywords
Cellular component	Host Golgi apparatus Host endosome Host membrane Host nucleus Membrane Viral envelope protein Virion
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	host cell Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell host cell endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell host cell nuclear inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW viral envelope Inferred from electronic annotation. Source: UniProtKB-KW virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 473	473	Envelope glycoprotein M		PRO_0000115775
Regions
	Topological domain	1 – 32	32	Intravirion Potential
	Transmembrane	33 – 53	21	Helical; Potential
	Topological domain	54 – 90	37	Virion surface Potential
	Transmembrane	91 – 111	21	Helical; Potential
	Topological domain	112 – 137	26	Intravirion Potential
	Transmembrane	138 – 158	21	Helical; Potential
	Topological domain	159 – 163	5	Virion surface Potential
	Transmembrane	164 – 184	21	Helical; Potential
	Topological domain	185 – 216	32	Intravirion Potential
	Transmembrane	217 – 237	21	Helical; Potential
	Topological domain	238 – 250	13	Virion surface Potential
	Transmembrane	251 – 271	21	Helical; Potential
	Topological domain	272 – 280	9	Intravirion Potential
	Transmembrane	281 – 301	21	Helical; Potential
	Topological domain	302 – 318	17	Virion surface Potential
	Transmembrane	319 – 339	21	Helical; Potential
	Topological domain	340 – 473	134	Intravirion Potential
Amino acid modifications
	Glycosylation	71	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	246	1	N-linked (GlcNAc...); by host Potential
Natural variations
	Natural variant	77	1	R → C in strain: Nonneuroinvasive mutant HF10.
	Natural variant	105	1	V → A in strain: Nonneuroinvasive mutant HF10.
	Natural variant	279	1	V → M in STRAIN: 17 syn+.
	Natural variant	448	1	D → E in strain: Nonneuroinvasive mutant HF10.
	Natural variant	462	1	A → T in strain: Nonneuroinvasive mutant HF10.

Sequences

	Sequence		Length	Mass (Da)
	P04288 [UniParc]. Last modified March 20, 1987. Version 1. Checksum: E08FD792409A81E6	FASTA	473	51,393
	10 20 30 40 50 60 MGRPAPRGSP DSAPPTKGMT GARTAWWVWC VQVATFVVSA VCVTGLLVLA SVFRARFPCF 70 80 90 100 110 120 YATASSYAGV NSTAEVRGGV AVPLRLDTQS LVGTYVITAV LLLAVAVYAV VGAVTSRYDR 130 140 150 160 170 180 ALDAGRRLAA ARMAMPHATL IAGNVCSWLL QITVLLLAHR ISQLAHLVYV LHFACLVYFA 190 200 210 220 230 240 AHFCTRGVLS GTYLRQVHGL MELAPTHHRV VGPARAVLTN ALLLGVFLCT ADAAVSLNTI 250 260 270 280 290 300 AAFNFNFSAP GMLICLTVLF AILVVSLLLV VEGVLCHYVR VLVGPHLGAV AATGIVGLAC 310 320 330 340 350 360 EHYYTNGYYV VETQWPGAQT GVRVALALVA AFALGMAVLR CTRAYLYHRR HHTKFFMRMR 370 380 390 400 410 420 DTRHRAHSAL KRVRSSMRGS RDGRHRPAPG SPPGIPEYAE DPYAISYGGQ LDRYGDSDGE 430 440 450 460 470 PIYDEVADDQ TDVLYAKIQH PRHLPDDDPI YDTVGGYDPE PAEDPVYSTV RRW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence of the region in the genome of herpes simplex virus type 1 containing the exonuclease gene and neighbouring genes." McGeoch D.J., Dolan A., Frame M.C. Nucleic Acids Res. 14:3435-3448(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1." McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P. J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Determination and analysis of the DNA sequence of highly attenuated herpes simplex virus type 1 mutant HF10, a potential oncolytic virus." Ushijima Y., Luo C., Goshima F., Yamauchi Y., Kimura H., Nishiyama Y. Microbes Infect. 9:142-149(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nonneuroinvasive mutant HF10.
[4]	"Herpes simplex virus type 1 bacterial artificial chromosome." Cunningham C., Davison A.J. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 17 syn+.
[5]	"Structures of herpes simplex virus type 1 genes required for replication of virus DNA." McGeoch D.J., Dalrymple M.A., Dolan A., McNab D., Perry L.J., Taylor P., Challberg M.D. J. Virol. 62:444-453(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-98.
[6]	"Glycoprotein M of herpes simplex virus 1 is incorporated into virions during budding at the inner nuclear membrane." Baines J.D., Wills E., Jacob R.J., Pennington J., Roizman B. J. Virol. 81:800-812(2007) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: F.
[7]	"Comprehensive characterization of extracellular herpes simplex virus type 1 virions." Loret S., Guay G., Lippe R. J. Virol. 82:8605-8618(2008) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: F.
[8]	"The U(L)31 and U(L)34 gene products of herpes simplex virus 1 are required for optimal localization of viral glycoproteins D and M to the inner nuclear membranes of infected cells." Wills E., Mou F., Baines J.D. J. Virol. 83:4800-4809(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: F.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X14112 Genomic DNA. Translation: CAA32346.1. X03839 Genomic DNA. Translation: CAA27451.1. DQ889502 Genomic DNA. Translation: ABI63472.1. FJ593289 Genomic DNA. Translation: ACM62232.1. M19120 Genomic DNA. Translation: AAA45821.2.
PIR	WMBE51. A03736.
RefSeq	NP_044611.1. NC_001806.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2703379.
Phylogenomic databases
ProtClustDB	PHA3242.
Family and domain databases
InterPro	IPR000785. Herpes_glycop. [Graphical view]
Pfam	PF01528. Herpes_glycop. 1 hit. [Graphical view]
PRINTS	PR00333. HSVINTEGRLMP.
ProtoNet	Search...

Entry information

Entry name

GM_HHV11

Accession

Primary (citable) accession number: P04288
Secondary accession number(s): B9VQD7, Q09IC3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 1987
Last sequence update:	March 20, 1987
Last modified:	April 3, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program